IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ A new Mint1 isoform, but not the conventional Mint1, interacts with the small GTPase Rab6. A new Mint1 isoform, but not the conventional Mint1, interacts with the small GTPase Rab6. PLoS ONE 2013 imex curation Thyrock A., Ossendorf E., Stehling M., Kail M., Kurtz T., Pohlentz G., Waschbüsch D., Eggert S., Formstecher E., Müthing J., Dreisewerd K., Kins S., Goud B., Barnekow A. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease Alzheimers - Interactions investigated in the context of Alzheimers disease NEW-MINT1 isoforms:Update when UniProt AC available.(- I also had to create a new internal protein - isoform 1 - in order to enter the negative interactions.) The revised entries should become publicly available as part of release 2014_01 due to January 22nd. (They will be as already entered here.) The authors confirm that Rab6B constructs/proteins are of human origin, and also that the species of transfected APP695 in Figure 7 and Figure 9C is human. barneko@uni-muenster.de 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Thyrock A., Ossendorf E., Stehling M., Kail M., Kurtz T., Pohlentz G., Waschbüsch D., Eggert S., Formstecher E., Müthing J., Dreisewerd K., Kins S., Goud B., Barnekow A. Parkinsons - Interactions investigated in the context of Parkinsons disease This experiment relates to negative interactions. NEW-MINT1 isoforms:Update when UniProt AC available.(- I also had to create a new internal protein - isoform 1 - in order to enter the negative interactions.) The revised entries should become publicly available as part of release 2014_01 due to January 22nd. (They will be as already entered here.) The authors confirm that Rab6B constructs/proteins are of human origin, and also that the species of transfected APP695 in Figure 7 and Figure 9C is human. barneko@uni-muenster.de Accepted 2013-NOV-21 by ORCHARD PLoS ONE 2013 Only protein-protein interactions imex curation A new Mint1 isoform, but not the conventional Mint1, interacts with the small GTPase Rab6. A new Mint1 isoform, but not the conventional Mint1, interacts with the small GTPase Rab6. PLoS ONE 2013 imex curation Thyrock A., Ossendorf E., Stehling M., Kail M., Kurtz T., Pohlentz G., Waschbüsch D., Eggert S., Formstecher E., Müthing J., Dreisewerd K., Kins S., Goud B., Barnekow A. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease Alzheimers - Interactions investigated in the context of Alzheimers disease NEW-MINT1 isoforms:Update when UniProt AC available.(- I also had to create a new internal protein - isoform 1 - in order to enter the negative interactions.) The revised entries should become publicly available as part of release 2014_01 due to January 22nd. (They will be as already entered here.) The authors confirm that Rab6B constructs/proteins are of human origin, and also that the species of transfected APP695 in Figure 7 and Figure 9C is human. barneko@uni-muenster.de 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag fluorescence tag visualisation by fluorescence tag fluorescence Thyrock A., Ossendorf E., Stehling M., Kail M., Kurtz T., Pohlentz G., Waschbüsch D., Eggert S., Formstecher E., Müthing J., Dreisewerd K., Kins S., Goud B., Barnekow A. Parkinsons - Interactions investigated in the context of Parkinsons disease This experiment relates to negative interactions. NEW-MINT1 isoforms:Update when UniProt AC available.(- I also had to create a new internal protein - isoform 1 - in order to enter the negative interactions.) The revised entries should become publicly available as part of release 2014_01 due to January 22nd. (They will be as already entered here.) FACS was used for fluorescence detection. The authors confirm that Rab6B constructs/proteins are of human origin, and also that the species of transfected APP695 in Figure 7 and Figure 9C is human. barneko@uni-muenster.de Accepted 2013-NOV-21 by ORCHARD PLoS ONE 2013 Only protein-protein interactions imex curation A new Mint1 isoform, but not the conventional Mint1, interacts with the small GTPase Rab6. A new Mint1 isoform, but not the conventional Mint1, interacts with the small GTPase Rab6. PLoS ONE 2013 imex curation Thyrock A., Ossendorf E., Stehling M., Kail M., Kurtz T., Pohlentz G., Waschbüsch D., Eggert S., Formstecher E., Müthing J., Dreisewerd K., Kins S., Goud B., Barnekow A. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease Alzheimers - Interactions investigated in the context of Alzheimers disease NEW-MINT1 isoforms:Update when UniProt AC available.(- I also had to create a new internal protein - isoform 1 - in order to enter the negative interactions.) The revised entries should become publicly available as part of release 2014_01 due to January 22nd. (They will be as already entered here.) The authors confirm that Rab6B constructs/proteins are of human origin, and also that the species of transfected APP695 in Figure 7 and Figure 9C is human. barneko@uni-muenster.de 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Thyrock A., Ossendorf E., Stehling M., Kail M., Kurtz T., Pohlentz G., Waschbüsch D., Eggert S., Formstecher E., Müthing J., Dreisewerd K., Kins S., Goud B., Barnekow A. Parkinsons - Interactions investigated in the context of Parkinsons disease This experiment relates to negative interactions. A Mint1 isoform1 PTB domain containing construct (aas 438-625) also did not bind Rab6 proteins, - same as for the full length Mint1 isoform. NEW-MINT1 isoforms:Update when UniProt AC available.(- I also had to create a new internal protein - isoform 1 - in order to enter the negative interactions.) The revised entries should become publicly available as part of release 2014_01 due to January 22nd. (They will be as already entered here.) The authors confirm that Rab6B constructs/proteins are of human origin, and also that the species of transfected APP695 in Figure 7 and Figure 9C is human. barneko@uni-muenster.de Accepted 2013-NOV-21 by ORCHARD PLoS ONE 2013 Only protein-protein interactions imex curation p20340-1 Ras-related protein Rab-6A Rab-6a RAB6A RAB6 protein protein human Homo sapiens Human MSTGGDFGNPLRKFKLVFLGEQSVGKTSLITRFMYDSFDNTYQATIGIDFLSKTMYLEDRTVRLQLWDTAGQERFRSLIPSYIRDSTVAVVVYDITNVNSFQQTTKWIDDVRTERGSDVIIMLVGNKTDLADKRQVSIEEGERKAKELNVMFIETSAKAGYNVKQLFRRVAAALPGMESTQDRSREDMIDIKLEKPQEQPVSEGGCSC DIP protein P20340-1 original sequence version: 121 DIP protein P20340 original sequence version: 125 DIP protein P20340 original sequence version: 149 C9A3C2DDBF6C3E9E rab6a_human Ras-related protein Rab-6A RAB6A RAB6 protein protein human Homo sapiens Human MSTGGDFGNPLRKFKLVFLGEQSVGKTSLITRFMYDSFDNTYQATIGIDFLSKTMYLEDRTVRLQLWDTAGQERFRSLIPSYIRDSTVAVVVYDITNVNSFQQTTKWIDDVRTERGSDVIIMLVGNKTDLADKRQVSIEEGERKAKELNVMFIETSAKAGYNVKQLFRRVAAALPGMESTQDRSREDMIDIKLEKPQEQPVSEGGCSC C9A3C2DDBF6C3E9E rab6b_human Ras-related protein Rab-6B RAB6B protein protein human Homo sapiens Human MSAGGDFGNPLRKFKLVFLGEQSVGKTSLITRFMYDSFDNTYQATIGIDFLSKTMYLEDRTVRLQLWDTAGQERFRSLIPSYIRDSTVAVVVYDITNLNSFQQTSKWIDDVRTERGSDVIIMLVGNKTDLADKRQITIEEGEQRAKELSVMFIETSAKTGYNVKQLFRRVASALPGMENVQEKSKEGMIDIKLDKPQEPPASEGGCSC E81C831996E2446D q02410-1 Amyloid-beta A4 precursor protein-binding family A member 1 APBA1 MINT1 X11 Adapter protein X11alpha Neuron-specific X11 protein Neuronal Munc18-1-interacting protein 1 protein protein human Homo sapiens Human MNHLEGSAEVEVTDEAAGGEVNESVEADLEHPEVEEEQQQPPQQQHYVGRHQRGRALEDLRAQLGQEEEERGECLARSASTESGFHNHTDTAEGDVIAAARDGYDAERAQDPEDESAYAVQYRPEAEEYTEQAEAEHAEATHRRALPNHLHFHSLEHEEAMNAAYSGYVYTHRLFHRGEDEPYSEPYADYGGLQEHVYEEIGDAPELDARDGLRLYEQERDEAAAYRQEALGARLHHYDERSDGESDSPEKEAEFAPYPRMDSYEQEEDIDQIVAEVKQSMSSQSLDKAAEDMPEAEQDLERPPTPAGGRPDSPGLQAPAGQQRAVGPAGGGEAGQRYSKEKRDAISLAIKDIKEAIEEVKTRTIRSPYTPDEPKEPIWVMRQDISPTRDCDDQRPMDGDSPSPGSSSPLGAESSSTSLHPSDPVEASTNKESRKSLASFPTYVEVPGPCDPEDLIDGIIFAANYLGSTQLLSDKTPSKNVRMMQAQEAVSRIKMAQKLAKSRKKAPEGESQPMTEVDLFISTQRIKVLNADTQETMMDHPLRTISYIADIGNIVVLMARRRMPRSNSQENVEASHPSQDGKRQYKMICHVFESEDAQLIAQSIGQAFSVAYQEFLRANGINPEDLSQKEYSDLLNTQDMYNDDLIHFSKSENCKDVFIEKQKGEILGVVIVESGWGSILPTVIIANMMHGGPAEKSGKLNIGDQIMSINGTSLVGLPLSTCQSIIKGLKNQSRVKLNIVRCPPVTTVLIRRPDLRYQLGFSVQNGIICSLMRGGIAERGGVRVGHRIIEINGQSVVATPHEKIVHILSNAVGEIHMKTMPAAMYRLLTAQEQPVYI 29C3620BBC89534B rab6b-apba1-3 3 7 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait P20340-1:p.Gln72Leu mutation mutation certain certain sequence position certain certain certain sequence position certain Q L Wed May 02 15:25:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln72leu' Tue Jun 30 19:40:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln72Leu' P20340-1:p.Thr27Asn mutation mutation certain certain sequence position certain certain certain sequence position certain T N Wed May 02 17:32:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr27asn' Tue Jun 30 18:40:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr27Asn' physical association physical association true TABLE 2 and 4 rab6b-apba1-3 3 6 unspecified role unspecified role bait bait Q9NRW1:p.Gln72Leu mutation mutation certain certain sequence position certain certain certain sequence position certain Q L Wed May 02 15:19:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln72leu' Tue Jun 30 19:54:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln72Leu' Q9NRW1:p.Thr27Asn mutation mutation certain certain sequence position certain certain certain sequence position certain T N Wed May 02 15:39:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr27asn' Tue Jun 30 17:34:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr27Asn' 7 unspecified role unspecified role prey prey physical association physical association true TABLE 2 and 4 q02410_2-rab6b-1 1 6 unspecified role unspecified role prey prey Q9NRW1:p.Thr27Asn mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain T N Wed May 02 15:26:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr27asn' Tue Jun 30 18:10:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr27Asn' Q9NRW1:p.Gln72Leu mutation mutation certain certain sequence position certain certain certain sequence position certain Q L Wed May 02 18:59:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln72leu' Tue Jun 30 18:23:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln72Leu' region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 7 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ptb/pi_domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association true Fig 2A q02410_2-rab6a-1 1 5 unspecified role unspecified role prey prey region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P20340:p.Thr27Asn mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain T N Wed May 02 17:06:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr27asn' Tue Jun 30 16:30:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr27Asn' P20340:p.Gln72Leu mutation mutation certain certain sequence position certain certain certain sequence position certain Q L Wed May 02 15:01:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln72leu' Tue Jun 30 17:50:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln72Leu' human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 7 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ptb/pi_domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association true Fig 2A q02410_2-rab6b-3 2 7 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n_terminus eyfp enhanced yellow fluorescent protein tag eyfp n-term range n-terminal range n-term range n-term range n-terminal range n-term range ptb/pi_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role fluorescence donor fluorescence donor c_terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q9NRW1:p.Gln72Leu mutation mutation certain certain sequence position certain certain certain sequence position certain Q L Wed May 02 15:53:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln72leu' Tue Jun 30 16:16:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln72Leu' physical association physical association true Fig 3 q02410_2-rab6a-5 2 7 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept ptb/pi_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus eyfp enhanced yellow fluorescent protein tag eyfp n-term range n-terminal range n-term range n-term range n-terminal range n-term range 4 unspecified role unspecified role fluorescence donor fluorescence donor c_terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P20340-1:p.Gln72Leu mutation mutation certain certain sequence position certain certain certain sequence position certain Q L Wed May 02 14:59:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln72leu' Tue Jun 30 16:53:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln72Leu' physical association physical association true Fig 3