IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Proteasome regulation by ADP-ribosylation. Proteasome regulation by ADP-ribosylation. Cell (0092-8674) 2013 imex curation Cho-Park P.F., Steller H. steller@rockefeller.edu 14-Oct-2013: Contacted by IntAct-Help. Only protein-protein interactions drome-embryo drosophilla melanogaster embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Cho-Park P.F., Steller H. steller@rockefeller.edu Accepted 2013-OCT-02 by PPORRAS 14-Oct-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation Proteasome regulation by ADP-ribosylation. Proteasome regulation by ADP-ribosylation. Cell (0092-8674) 2013 imex curation Cho-Park P.F., Steller H. steller@rockefeller.edu 14-Oct-2013: Contacted by IntAct-Help. Only protein-protein interactions drome-embryo drosophilla melanogaster embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. far western blotting far western blotting Affinity blotting Affinity blotting weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Cho-Park P.F., Steller H. Expt 1 - data not shown but Margaret and I agreed it was OK as a screen - two of the interactors are described later in the text. I have not included the in vitro ADP ribosylation (Fig. 2B) because he gel does not seem to tell the same story as the text - you only seem to get ribosylation in the track with no enzyme. No pre-purification of riibosomes in Fig. 5 and S3 so have not included density sedimentation data. steller@rockefeller.edu Accepted 2013-OCT-02 by PPORRAS 14-Oct-2013: Contacted by IntAct-Help. Drosophila lambda cDNA Cell (0092-8674) 2013 Only protein-protein interactions imex curation Proteasome regulation by ADP-ribosylation. Proteasome regulation by ADP-ribosylation. Cell (0092-8674) 2013 imex curation Cho-Park P.F., Steller H. steller@rockefeller.edu 14-Oct-2013: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Cho-Park P.F., Steller H. steller@rockefeller.edu Accepted 2013-OCT-02 by PPORRAS 14-Oct-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation psmd9_drome 26S proteasome non-ATPase regulatory subunit 9 26S proteasome regulatory subunit p27 CG9588 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MAAGTTTKERLERLINAKKQLEAQINRNGQILAANDNVGMSGPLVDAEGFPRNDIDVYQVRLARQTIICLQNDHKELMNQIQTLLNQYHSEIATTDPELVNRASALDLDSDRSPGGANITDLAPARAIVVVNLVSPDSPAERAGLCAGDAILRFGSINSGNFKGDLAQIGELVRNMQSQNVQLKVKRGEQQLDLILVPKTWSGRGLLGCNIVLPPEAMDH C618E8D04262293A psa1_drome Proteasome subunit alpha type-1 Prosalpha6 PROS-35 PROS-Dm35 Pros35 Proteasome 35 kDa subunit CG4904 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MFRNQYDSDVTVWSPQGRLHQVEYAMEAVKLGTATVGLKNKDYAVLVALCKPTSELSDTQRKIIPIDDHLGISIAGLTADARVLSRYLRSECLNYKHSYDTTYPVSRLITNLGNKMQTTTQRYDRRPYGVGLLVAGYDERGPHIYQVTPSATFFNCKANSIGSRSQSARTYLEKNLNKFLDSSKDEIIRHGIRAILGTLPTDEQGKDAGQYDITVAIVGKDQPFTILSNKDSAKHVAIAKENDNDTPRNDDDDDRPSPPEEPAAGPRDPEVLVATEQRP 1478BDC2C61EF3F1 q9xzc3_drome Rpt5 Tbp-1 CG10370 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MVLDMAQTLEDKSIWEDGEESLGEKVMRMSTDEIVSRTRLMDNEIKIMKSEVIRITHEIQAQNEKIKDNTEKIKVNKTLPYLVSNVIELLDVDPQEEEDDGSVTVLDNQRKGKCAVIKTSTRQAYFLPVIGLVDAEKLKPGDLAGVNKDFYLILETLPAEYDARVKAMEVDERPTEQFSDIGGLDKQIQELIEAVVLPMTHKEKFKNLGIHPPKGVILYGPPGTGKTLLARACAAQTKSTFLKLAGPQLVQMFIGDGAKLVRDAFALAKEKAPAIIFIDELDAIGTKRFDSEKAGDREVQRTMLELLNQLNGFSSTADIKVIAATNRVDILPALLRSGRLDRKIEFPHPNEEARARIMQIHSRKMNVSNDVNFEELSRSTDDFNGAQCKAVCVEAGMIALRRSANSVTHEDFMDAIMEVQAKKKANLNYYA BBF763A0FC6B28F2 psmf1_drome Proteasome inhibitor PI31 subunit PI31 CG8979 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MESGSTAKTGDFFYGWDLLYKTVKADVSKKSDLLIALVHFLLTKHYNFRCVGVGDDKTLPEEEGSELLPDSWNDDDTKYSLRYVHDKMLYLLLGHITEGSLLINLLDINTKKVSNICVEPETLVPEVKGGITTIMPSASEIVERYRRELLDPVFTGNSREVTTQTTNSPRPIGSDPDPLRIGEPRRGGSFIPSAFEPRPFGFPDVGRGDLDPLGRGGHGNLFSFPSRPNMGPGPVPRFDPFNPLNPNRPGQGGINPDHMRPPDWNPDYYM F7972E7634E80555 psa4_drome Proteasome subunit alpha type-4 Prosalpha3 PROS-29 PROS-Dm29 Pros29 Proteasome 29 kDa subunit CG9327 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MARRYDSRTTIFSPEGRLYQVEYAMEAISHAGTCLGILAEDGILLAAECRSTNKLLDSAIPSEKIYRLNDNMVCSVAGITSDANVLTSELRLIAQRYQFSYGEVIPCEQLVSHLCDIKQAYTQYGGKRPFGVSLLYMGWDNKYGYQLYQSDPSGNYGGWKATCIGNNFGAAISMLKQELADKENVKLTLADAKDLAIKVLSMTLDTTKLTPEKVEMATLQRVDNKTVYSVLEKPDVEKLIEKYTKVQAEAEAAKKEKQAKQPTK E5209BC634008B8B psmd5_drome 26S proteasome non-ATPase regulatory subunit 5 26S proteasome subunit S5B CG12096 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MEMEDNWWVTKLKALESKPQRLDALTAMNSAIAKEAALPRQIIERLLTTDQLYDCANPAADSHVPKDQAVDVTLELLCHCLDQLAMDTADEQLSSLLRRGLTHSNPALRAQVLASLFKKLLRQLTVGQVLTLPNNELIFLILDELKQPDTQSTSLAINILSIVLPQRISNADVQAKLVQLLKQNEIVRCRAYELAVVLAKKSATLLSDVTFILDAALSELDNDDVLLQASVMELLVPLAEQNHGLSYMERRRVLDIISYRVQRVEEHPLDALLVPSIMKFFGKISVYQPLKIIGGYPHMLACLFMQLQSEDESILPTAMDTLANLATTPQGKILLNMHFSGAMEKSFKKYGSHTKKLSAHIKKRLLNSLDVIYDFKTPPATEIINIGKNWYECFAGGAHANIIMDLINTPFPDLQMAALSFLKTICKYNWGIVALKNTGGAVEFLLSRQKDLHRDIKYMKWQIMEILSASAEFSPTETIRFTAYVNEGPYHVQADLDVATEPQGNA FDD4CA3103FDAD33 psa3_drome Proteasome subunit alpha type-3 Prosalpha7 CG1519 20S proteasome subunit alpha-7 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSTIGTGYDLSASQFSPDGRVFQIDYASKAVEKSGTVIGIRGKDAVVLAVEKIITSKLYEPDAGGRIFTIEKNIGMAVAGLVADGNFVADIARQEAANYRQQFEQAIPLKHLCHRVAGYVHAYTLYSAVRPFGLSIILASWDEVEGPQLYKIEPSGSSFGYFACASGKAKQLAKTEMEKLKMDMRTDELVESAGEIIYKVHDELKDKDFRFEMGLVGRVTGGLHLINPSELTEKARKAGDAANKDEDSDNETH 7B19D8DA350E2B7E psa6_drome Proteasome subunit alpha type-6 Prosalpha1 Prosalpha6 20S proteasome subunit alpha-1 CG18495 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSRGSSAGFDRHITIFSPEGRLYQVEYAFKAIAQENITTVALKSGDCAVVATQKKVTEKNIVPETVTHLFRITKDIGCAMTGRIADSRSQVQKARYEAANFRYKYGYEMPVDVLCRRIADINQVYTQNAEMRPLGCSMVLIAYDNEIGPSVYKTDPAGYFSGFKACSVGAKTLEANSYLEKKYKPNLSEEKAIQLAISCLSSVLAIDFKPNGIEIGVVSKSDPTFRILDEREIEEHLTKIAEKD D217B272CA339D05 tnks_drome Poly [ADP-ribose] polymerase tankyrase Tnks tankyrase CG4719 Poly [ADP-ribose] polymerase Protein poly-ADP-ribosyltransferase tankyrase protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MANSSRSRAILSVNLDAVMANDPLRELFEACKTGEIAKVKKLITPQTVNARDTAGRKSTPLHFAAGYGRREVVEFLLNSGASIQACDEGGLHPLHNCCSFGHAEVVRLLLKAGASPNTTDNWNYTPLHEAASKGKVDVCLALLQHGANHTIRNSEQKTPLELADEATRPVLTGEYRKDELLEAARSGAEDRLLALLTPLNVNCHASDGRRSTPLHLAAGYNRIGIVEILLANGADVHAKDKGGLVPLHNACSYGHFDVTKLLIQAGANVNANDLWAFTPLHEAASKSRVEVCSLLLSRGADPTLLNCHSKSAIDAAPTRELRERIAFEYKGHCLLDACRKCDVSRAKKLVCAEIVNFVHPYTGDTPLHLAVVSPDGKRKQLMELLTRKGSLLNEKNKAFLTPLHLAAELLHYDAMEVLLKQGAKVNALDSLGQTPLHRCARDEQAVRLLLSYAADTNIVSLEGLTAAQLASDSVLKLLKNPPDSETHLLEAAKAGDLDTVRRIVLNNPISVNCRDLDGRHSTPLHFAAGFNRVPVVQFLLEHGAEVYAADKGGLVPLHNACSYGHYEVTELLVKHGANVNVSDLWKFTPLHEAAAKGKYDICKLLLKHGADPMKKNRDGATPADLVKESDHDVAELLRGPSALLDAAKKGNLARVQRLVTPESINCRDAQGRNSTPLHLAAGYNNFECAEYLLENGADVNAQDKGGLIPLHNASSYGHLDIAALLIKHKTVVNATDKWGFTPLHEAAQKGRTQLCSLLLAHGADAYMKNQEGQTPIELATADDVKCLLQDAMATSLSQQALSASTQSLTSSSPAPDATAAAAPGTSSSSSSAILSPTTETVLLPTGASMILSVPVPLPLSSSTRISPAQGAEANGAEGSSSDDLLPDADTITNVSGFLSSQQLHHLIELFEREQITLDILAEMGHDDLKQVGVSAYGFRHKILKGIAQLRSTTGIGNNVNLCTLLVDLLPDDKEFVAVEEEMQATIREHRDNGQAGGYFTRYNIIRVQKVQNRKLWERYAHRRQEIAEENFLQSNERMLFHGSPFINAIVQRGFDERHAYIGGMFGAGIYFAEHSSKSNQYVYGIGGGIGCPSHKDKSCYVCPRQLLLCRVALGKSFLQYSAMKMAHAPPGHHSVVGRPSAGGLHFAEYVVYRGEQSYPEYLITYQIVKPDDSSSGTEDTR EB6F0F94E6A3D504 psmf1-tankyrase 2 7 unspecified role unspecified role bait bait hmk tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role prey prey physical association physical association psmf1-tankyrase-1 1 7 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey physical association physical association Fig. 1B tankyrase-psmf1 3 7 unspecified role unspecified role prey prey Q9V637:p.Leu210Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain L A Wed May 02 15:54:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu210ala' Tue Jun 30 20:22:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu210Ala' region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q9V637:p.[Arg49Ala;Gly54Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain G A Wed May 02 15:44:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg49ala,gly54ala' Tue Jun 30 20:10:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg49Ala;Gly54Ala]' Q9V637:p.Phe241Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain F A Wed May 02 17:35:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe241ala' Tue Jun 30 16:13:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe241Ala' hbyx domain required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 12 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 1C-G pros35-psmf1 3 5 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 4A prosalpha7-psmf1 3 10 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 4A pros29-psmf1 3 7 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 4A prosalpha1-psmf1 3 11 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 4A pros29-psmf1-1 3 8 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 4B Unmodified His-PI31 strongly interacted with alpha-4, whereas binding of the ADP-ribosylated version was significantly diminished psmf1-q9vfs8 1 4 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association Fig. 6A psmf1-q9vfs8-1 2 4 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait hmk tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association psmf1-cg12096_ra 2 7 unspecified role unspecified role bait bait hmk tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey physical association physical association q9vfs8-psmf1 3 4 unspecified role unspecified role bait bait Q9VFS8:p.Arg187Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain R A Wed May 02 15:33:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg187ala' Tue Jun 30 18:23:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg187Ala' Q9VFS8:p.Glu9Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain E A Wed May 02 18:54:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu9ala' Tue Jun 30 19:51:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu9Ala' region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined hbyx domain required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain Q9V637:p.Phe241Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain F A Wed May 02 17:57:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe241ala' Tue Jun 30 16:04:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe241Ala' Q9V637:p.Leu210Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain L A Wed May 02 16:50:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu210ala' Tue Jun 30 16:16:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu210Ala' physical association physical association Fig. 6C, 6E cg12096_ra-psmf1 3 9 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q9VYG1:p.Glu44Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain E A Wed May 02 15:59:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu44ala' Tue Jun 30 19:03:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu44Ala' Q9VYG1:p.Arg263Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain R A Wed May 02 19:39:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg263ala' Tue Jun 30 17:50:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg263Ala' 7 unspecified role unspecified role prey prey hbyx domain required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain Q9V637:p.Leu210Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain L A Wed May 02 15:44:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu210ala' Tue Jun 30 18:47:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu210Ala' Q9V637:p.Phe241Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain F A Wed May 02 20:02:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe241ala' Tue Jun 30 19:53:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe241Ala' region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp Fig. 5B, 5C q9vfs8-rpt5 3 4 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 7A, 7B ADP-ribosylation considerably increased the ability of PI31 to recruit the assembly chaperones when compared to its unmodified version, sequestering them away from the Rpt base subunits in a dose-dependent manner cg12096_ra-rpt5 3 9 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp. Fig. 6A ADP-ribosylation considerably increased the ability of PI31 to recruit the assembly chaperones when compared to its unmodified version, sequestering them away from the Rpt base subunits in a dose-dependent manner cg12096_ra-rpt5-1 3 9 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp. Fig. 6B