IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Protein complexes are under evolutionary selection to assemble via ordered pathways Protein complexes are under evolutionary selection to assemble via ordered pathways Cell (0092-8674) 2013 imex curation Marsh J.A., Hernández H., Hall Z., Ahnert S.E., Perica T., Robinson C.V., Teichmann S.A. 2013-02-13: J. Marsh, European Bioinformatics Institute, Hinxton, UK Data on :\data\curation-material\2013\marsh-2013 jmarsh@ebi.ac.uk, sarah.teichmann@ebi.ac.uk 16-Apr-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro ms of complexes mass spectrometry studies of complexes ms of complexes predetermined predetermined participant predetermined Marsh J.A., Hernández H., Hall Z., Ahnert S.E., Perica T., Robinson C.V., Teichmann S.A. 2013-02-13: J. Marsh, European Bioinformatics Institute, Hinxton, UK Data on :\data\curation-material\2013\marsh-2013 jmarsh@ebi.ac.uk, sarah.teichmann@ebi.ac.uk Accepted 2013-FEB-20 by PPORRAS 16-Apr-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation ure2_kleae Urease subunit beta ureB Urea amidohydrolase subunit beta protein protein entae Enterobacter aerogenes Aerobacter aerogenes MIPGEYHVKPGQIALNTGRATCRVVVENHGDRPIQVGSHYHFAEVNPALKFDRQQAAGYRLNIPAGTAVRFEPGQKREVELVAFAGHRAVFGFRGEVMGPLEVNDE A232BFCD74864EA6 trpb_salty Tryptophan synthase beta chain trpB STM1726 protein protein salty Salmonella typhimurium MTTLLNPYFGEFGGMYVPQILMPALNQLEEAFVSAQKDPEFQAQFADLLKNYAGRPTALTKCQNITAGTRTTLYLKREDLLHGGAHKTNQVLGQALLAKRMGKSEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVHSGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILDKEGRLPDAVIACVGGGSNAIGMFADFINDTSVGLIGVEPGGHGIETGEHGAPLKHGRVGIYFGMKAPMMQTADGQIEESYSISAGLDFPSVGPQHAYLNSIGRADYVSITDDEALEAFKTLCRHEGIIPALESSHALAHALKMMREQPEKEQLLVVNLSGRGDKDIFTVHDILKARGEI DIP protein P0A2K1 original sequence version: 72 DIP protein P0A2K1 original sequence version: 62 73D210F5C6F2FEBD ure3_kleae Urease subunit gamma ureA Urea amidohydrolase subunit gamma protein protein entae Enterobacter aerogenes Aerobacter aerogenes MELTPREKDKLLLFTAALVAERRLARGLKLNYPESVALISAFIMEGARDGKSVASLMEEGRHVLTREQVMEGVPEMIPDIQVEATFPDGSKLVTVHNPII C5B9E7FAF615C04D trpa_salty Tryptophan synthase alpha chain trpA STM1727 protein protein salty Salmonella typhimurium MERYENLFAQLNDRREGAFVPFVTLGDPGIEQSLKIIDTLIDAGADALELGVPFSDPLADGPTIQNANLRAFAAGVTPAQCFEMLALIREKHPTIPIGLLMYANLVFNNGIDAFYARCEQVGVDSVLVADVPVEESAPFRQAALRHNIAPIFICPPNADDDLLRQVASYGRGYTYLLSRSGVTGAENRGALPLHHLIEKLKEYHAAPALQGFGISSPEQVSAAVRAGAAGAISGSAIVKIIEKNLASPKQMLAELRSFVSAMKAASRA DIP protein P00929 original sequence version: 136 DIP protein P00929 original sequence version: 128 F409BF1A931581B5 carb_ecoli Carbamoyl-phosphate synthase large chain carB pyrA b0033 Carbamoyl-phosphate synthetase ammonia chain JW0031 protein protein ecoli Escherichia coli (strain K12) MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRVILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALELERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAADVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEMEVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIGVETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDELMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGADRIWYIADAFRAGLSVDGVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNADFLRQLKRKGFADARLAKLAGVREAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLGGGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLEDVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAVERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQTAVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPAYTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKATGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRSTGEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELDATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIRRSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK DIP protein P00968 original sequence version: 118 DIP protein P00968 original sequence version: 143 DIP protein P00968 original sequence version: null A09D019CBDFF8D2B ure1_kleae Urease subunit alpha ureC Urea amidohydrolase subunit alpha protein protein entae Enterobacter aerogenes Aerobacter aerogenes MSNISRQAYADMFGPTVGDKVRLADTELWIEVEDDLTTYGEEVKFGGGKVIRDGMGQGQMLAADCVDLVLTNALIVDHWGIVKADIGVKDGRIFAIGKAGNPDIQPNVTIPIGAATEVIAAEGKIVTAGGIDTHIHWICPQQAEEALVSGVTTMVGGGTGPAAGTHATTCTPGPWYISRMLQAADSLPVNIGLLGKGNVSQPDALREQVAAGVIGLKIHEDWGATPAAIDCALTVADEMDIQVALHSDTLNESGFVEDTLAAIGGRTIHTFHTEGAGGGHAPDIITACAHPNILPSSTNPTLPYTLNTIDEHLDMLMVCHHLDPDIAEDVAFAESRIRRETIAAEDVLHDLGAFSLTSSDSQAMGRVGEVILRTWQVAHRMKVQRGALAEETGDNDNFRVKRYIAKYTINPALTHGIAHEVGSIEVGKLADLVVWSPAFFGVKPATVIKGGMIAIAPMGDINASIPTPQPVHYRPMFGALGSARHHCRLTFLSQAAAANGVAERLNLRSAIAVVKGCRTVQKADMVHNSLQPNITVDAQTYEVRVDGELITSEPADVLPMAQRYFLF 2ECF3C4268A2E2C7 d3uj81_helm1 Urease ureA2 HMU13020 protein protein helm1 Helicobacter mustelae (strain ATCC 43772 / LMG 18044 / NCTC 12198 / 12198) Campylobacter mustelae MKLTPKEQEKFLLYYAGEVARKRKEEGLKLNQPEAIAYISAHIMDEARRGKKTVAQLMEECVHFLKKDEVMPGVGNMVPDLGVEANFPDGTKLVTVNWPIEPDDFKAGEIKFASDKDIELNAGKEITELKVTNKGPKSLHVGSHFHFFEANRALEFDREKAYGKRLDIPSGNTLRIGAGETKTVHLIPIGGSKKIIGMNGLLNGIADDLHKQKALEKAKHHGFIK DIP protein D3UJ81 original sequence version: 15 92954DECE6D459FD acca_ecoli Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha accA b0185 JW0180 protein protein ecoli Escherichia coli (strain K12) MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLREKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIVGGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITFIDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVNMLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAHRNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA DIP protein P0ABD5 original sequence version: 47 DIP protein P0ABD5 original sequence version: 71 A810DE891CEA2B4F d3uj80_helm1 Urease subunit beta ureB Urea amidohydrolase subunit beta ureB2 HMU13010 protein protein helm1 Helicobacter mustelae (strain ATCC 43772 / LMG 18044 / NCTC 12198 / 12198) Campylobacter mustelae MKMKRQEYVNTYGPTTGDKVRLGDTDLWAEVEHDYTVYGEELKFGAGKTIREGMGQSNSPDENTLDLVITNALIIDYTGIYKADIGIKNGKIHGIGKAGNKDMQDGVTPHMVVGVGTEALAGEGMIITAGGIDSHTHFLSPQQFPTALANGVTTMFGGGTGPVDGTNATTITPGVWNLHRMLRAAEEYGMNVGLLGKGNSSSRAQLVEQVKAGAIGFKLHEDWGTTPSAIDHCLSVADEYDVQVCIHTDTVNEAGYVDDTLRAMNGRAIHAYHIEGAGGGHSPDVITMAGEVNILPSSTTPTIPYTINTVAEHLDMLMTCHHLDKRIREDLQFSQSRIRPGSIAAEDTLHDMGVIAMTSSDSQAMGRAGEVIPRTWQTADKNKKEFGRLTEEKGDNDNFRIKRYISKYTINPAITHGVSEYIGSVEEGKIADLVVWNPAFFGVKPKIIIKGGMVVFSEMGDSNASVPTPQPVYYREMFGHHGKAKFDTSITFVSKVAYENGIKEKLGLERKVLPVKNCRNVTKKDFKFNNTTAKITVNPETFEVFVNGKLCTSKPATEVALASRYTFF DIP protein D3UJ80 original sequence version: 21 74E06B993A43824A cara_ecoli Carbamoyl-phosphate synthase small chain carA pyrA b0032 Carbamoyl-phosphate synthetase glutamine chain JW0030 protein protein ecoli Escherichia coli (strain K12) MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYLKRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAKEVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTIVPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLALASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFDGTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK DIP protein P0A6F1 original sequence version: 58 DIP protein P0A6F1 original sequence version: 82 60BC26366417443F accd_ecoli Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta accD dedB usg b2316 JW2313 protein protein ecoli Escherichia coli (strain K12) MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLEVCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKETGEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFSASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNIAEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAKLMNLPAPNPEAPREGVVVPPVPDQEPEA DIP protein P0A9Q5 original sequence version: 42 DIP protein P0A9Q5 original sequence version: 66 401FEC94D728F3CB cara-carb-7 1 11 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli 6 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli direct interaction direct interaction Supplementary Table 2 cara-carb-8 1 6 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli 11 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli direct interaction direct interaction Supplementary Table 2 cara-carb-9 1 6 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli 11 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli direct interaction direct interaction Supplementary Table 2 cara-carb-10 1 11 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli 6 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli direct interaction direct interaction Supplementary Table 2 trpa-trpb-22 1 5 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli 3 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli direct interaction direct interaction Supplementary Table 2 trpa-trpb-23 1 5 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli 3 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli direct interaction direct interaction Supplementary Table 2 trpb-trpb 1 3 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli direct interaction direct interaction Supplementary Table 2 acca-accd-5 1 12 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 9 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction acca-acca 1 9 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 12 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 9 unspecified role unspecified role neutral component neutral component n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli physical association physical association Supplementary Table 2 acca-accd 1 12 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 9 unspecified role unspecified role neutral component neutral component n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction Supplementary Table 2 urea-ureb-7 1 7 unspecified role unspecified role neutral component neutral component entae Enterobacter aerogenes Aerobacter aerogenes 2 unspecified role unspecified role neutral component neutral component entae Enterobacter aerogenes Aerobacter aerogenes 4 unspecified role unspecified role neutral component neutral component entae Enterobacter aerogenes Aerobacter aerogenes physical association physical association Supplementary Table 2 urea-ureb-8 1 2 unspecified role unspecified role neutral component neutral component entae Enterobacter aerogenes Aerobacter aerogenes 4 unspecified role unspecified role neutral component neutral component entae Enterobacter aerogenes Aerobacter aerogenes 7 unspecified role unspecified role neutral component neutral component entae Enterobacter aerogenes Aerobacter aerogenes physical association physical association Supplementary Table 2 urea-ureb-9 1 7 unspecified role unspecified role neutral component neutral component entae Enterobacter aerogenes Aerobacter aerogenes 4 unspecified role unspecified role neutral component neutral component entae Enterobacter aerogenes Aerobacter aerogenes 2 unspecified role unspecified role neutral component neutral component entae Enterobacter aerogenes Aerobacter aerogenes physical association physical association Supplementary Table 2 urea-ureb-10 1 4 unspecified role unspecified role neutral component neutral component entae Enterobacter aerogenes Aerobacter aerogenes 7 unspecified role unspecified role neutral component neutral component entae Enterobacter aerogenes Aerobacter aerogenes direct interaction direct interaction Supplementary Table 2 urea-ureb-11 1 2 unspecified role unspecified role neutral component neutral component entae Enterobacter aerogenes Aerobacter aerogenes 7 unspecified role unspecified role neutral component neutral component entae Enterobacter aerogenes Aerobacter aerogenes 4 unspecified role unspecified role neutral component neutral component entae Enterobacter aerogenes Aerobacter aerogenes physical association physical association Supplementary Table 2 urea-1 1 7 unspecified role unspecified role neutral component neutral component entae Enterobacter aerogenes Aerobacter aerogenes physical association physical association Supplementary Table 2 urec 1 4 unspecified role unspecified role neutral component neutral component entae Enterobacter aerogenes Aerobacter aerogenes direct interaction direct interaction Supplementary Table 2 urea2-ureb2 1 10 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli 8 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli direct interaction direct interaction Supp. Table 2 urea2-ureb2-1 1 8 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli 10 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli direct interaction direct interaction Supp. Table 2 urea2-ureb2-2 1 8 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli 10 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli direct interaction direct interaction Supp. Table 2 urea2-ureb2-3 1 10 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli 8 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli direct interaction direct interaction Supp. Table 2 urea2-ureb2-4 1 8 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli 10 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli direct interaction direct interaction Supp. Table 2 urea2-ureb2-5 1 8 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli 10 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli direct interaction direct interaction Supp. Table 2 urea2-ureb2-6 1 8 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli 10 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli direct interaction direct interaction Supp. Table 2 urea2-ureb2-7 1 8 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli 10 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli direct interaction direct interaction Supp. Table 2 urea2-ureb2-8 1 10 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli direct interaction direct interaction Supp. Table 2 urea2-ureb2-9 1 8 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli direct interaction direct interaction Supp. Table 2 urea2-10 1 8 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli direct interaction direct interaction Supp. Table 2 urea2-ureb2-10 1 10 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli 8 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli direct interaction direct interaction Supp. Table 2 acca-accd-6 1 12 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 9 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction Supp. Table 2 acca-accd-7 1 12 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 9 unspecified role unspecified role neutral component neutral component n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction Supplementary Table 2