MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Evolutionary and mechanistic insights into substrate and product accommodation of CTP:phosphocholine cytidylyltransferase from Plasmodium falciparum. Evolutionary and mechanistic insights into substrate and product accommodation of CTP:phosphocholine cytidylyltransferase from Plasmodium falciparum. FEBS J. (1742-464X) 2013 Nagy GN., Marton L., Krámos B., Oláh J., Révész A., Vékey K., Delsuc F., Hunyadi-Gulyás E., Medzihradszky KF., Lavigne M., Vial H., Cerdan R., Vértessy BG. suggested by lperfetto gnagy@enzim.hu in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Nagy GN., Marton L., Krámos B., Oláh J., Révész A., Vékey K., Delsuc F., Hunyadi-Gulyás E., Medzihradszky KF., Lavigne M., Vial H., Cerdan R., Vértessy BG. suggested by lperfetto gnagy@enzim.hu FEBS J. (1742-464X) 2013 Evolutionary and mechanistic insights into substrate and product accommodation of CTP:phosphocholine cytidylyltransferase from Plasmodium falciparum. Evolutionary and mechanistic insights into substrate and product accommodation of CTP:phosphocholine cytidylyltransferase from Plasmodium falciparum. FEBS J. (1742-464X) 2013 Nagy GN., Marton L., Krámos B., Oláh J., Révész A., Vékey K., Delsuc F., Hunyadi-Gulyás E., Medzihradszky KF., Lavigne M., Vial H., Cerdan R., Vértessy BG. suggested by lperfetto gnagy@enzim.hu in vitro In vitro ms of complexes mass spectrometry studies of complexes ms of complexes experimental particp experimental participant identification experimental particp Nagy GN., Marton L., Krámos B., Oláh J., Révész A., Vékey K., Delsuc F., Hunyadi-Gulyás E., Medzihradszky KF., Lavigne M., Vial H., Cerdan R., Vértessy BG. suggested by lperfetto gnagy@enzim.hu FEBS J. (1742-464X) 2013 Evolutionary and mechanistic insights into substrate and product accommodation of CTP:phosphocholine cytidylyltransferase from Plasmodium falciparum. Evolutionary and mechanistic insights into substrate and product accommodation of CTP:phosphocholine cytidylyltransferase from Plasmodium falciparum. FEBS J. (1742-464X) 2013 Nagy GN., Marton L., Krámos B., Oláh J., Révész A., Vékey K., Delsuc F., Hunyadi-Gulyás E., Medzihradszky KF., Lavigne M., Vial H., Cerdan R., Vértessy BG. suggested by lperfetto gnagy@enzim.hu in vitro In vitro comigration in gel comigration in gel electrophoresis comigration in gel predetermined predetermined participant predetermined Nagy GN., Marton L., Krámos B., Oláh J., Révész A., Vékey K., Delsuc F., Hunyadi-Gulyás E., Medzihradszky KF., Lavigne M., Vial H., Cerdan R., Vértessy BG. suggested by lperfetto gnagy@enzim.hu FEBS J. (1742-464X) 2013 q8iee9_plaf7 PF3D7_1316600 protein protein plaf7 Plasmodium falciparum (isolate 3D7) MIIKVNSVQTQKSQDGNKVHNSQDDMDNEEKSIRIYADGVYDLLHLGHMKQLEQAKHVDKNVTLIVGVTGDNETRKFKGQIVQTLEERTETLKHIRWVDEIISPCPWVITPEFMEEHKIDYVAHDDIPYANNQKKKKKKKSKGKSFSFDEENEDIYAWLKRAGKFKATQRTEGVSTTDLIVRILKNYEDYIERSLQRGIHPNELNIGVTKAQSIKMKKNLIRWGEKVTDELTKVTLTDKPLGTDFDQGIDIIRDKVHDLFKLWRYHSKKLLKDFAKSFDPMFIIIRKRYRKDNLSAMYLSDSNYFSSSMKDELKKKKSLSSTFNNIDEYYYSADEEDTRDNSFYRVINKIANHTYYSEKENYETCFELEHSLRDKKKSLIFKDKNMKLSPDDLLHFTNPMNSKYDHNYYYYNNTYDKLSNVTFNLNGIKKKSYDNESSYIKTAYHDIIDEDKNLLNKEKSFNKDEKKKKKSILETDQYKNCEDQQFDNKYNHIIIGKKKSDSTYDNSLDKESSNEKKLSIDKGQYSNMDSSNYFHDCKTMLSEHNESIESSNNDINGKQKEHIKKGNSENQDVDPDTNPDAVPDDDDDDDNSNDESEYESSQMDSEKNKGSIKNSKNVVIYADGVYDMLHLGHMKQLEQAKKLFENTTLIVGVTSDNETKLFKGQVVQTLEERTETLKHIRWVDEIISPCPWVVTPEFLEKYKIDYVAHDDIPYANNQKKKKKKKSKGKSFSFDEENEDIYAWLKRAGKFKATQRTEGVSTTDLIVRILKNYEDYIERSLQRGIHPNELNIGVTKAQSIKMKKNLIRWGEKVTDELTKVTLTDKPLGTDFDQGVENLQVKFKELFKIWKNASNKLITDFTRKLEATSYLTSIQNIIDYEIENDDYASSNFDDETSS C3CCA9D4A1025DDB ctp-ctp-1 1 PfCCT MΔK 4 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined PfCCT MΔK 4 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction sf3 mint Gel filtration of PfCCT MdeltaK under native conditions resulted in a single peak, at an elution volume corresponding to a molecular mass of approximately 268 kDa (Fig. S3) ctp-ctp-2 3 PfCCT MΔK 4 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain PfCCT MΔK 4 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction f4a mint Denaturing SDS PAGE analysis revealed that this peak contains one polypeptide component for which the apparent molecular mass based on the SDS PAGE analysis is estimated to be 37 kDa (Fig. 4A), again considerably higher that the calculated molecular mass of 30.9 kDa. SDS-PAGE and gel filtration data therefore indicate anomalous migration of the protein in gel electrophoresis ctp-1 2 PfCCT MΔK 4 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain Stoichiometry: 2.0 direct interaction direct interaction f4b mint The electrospray mass spectrum of PfCCT MJK under native conditions (Fig. 4B) reveals two abundant ion series in the mass range m/z 1900-4000. In the upper mass range (m/z 3000-4000), peaks corresponding to a dimer structure can be observed.