MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it IRAK-M mediates Toll-like receptor/IL-1R-induced NFκB activation and cytokine production. IRAK-M mediates Toll-like receptor/IL-1R-induced NFκB activation and cytokine production. EMBO J. (0261-4189) 2013 imex curation Zhou H., Yu M., Fukuda K., Im J., Yao P., Cui W., Bulek K., Zepp J., Wan Y., Kim T.W., Yin W., Ma V., Thomas J., Gu J., Wang J.A., DiCorleto P.E., Fox P.L., Qin J., Li X. lix@ccf.org 10-Mar-2014: Contacted by IntAct-Help. Only protein-protein interactions mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zhou H., Yu M., Fukuda K., Im J., Yao P., Cui W., Bulek K., Zepp J., Wan Y., Kim T.W., Yin W., Ma V., Thomas J., Gu J., Wang J.A., DiCorleto P.E., Fox P.L., Qin J., Li X. lix@ccf.org Accepted 2014-MAR-03 by MARTA EMBO J. (0261-4189) 2013 Only protein-protein interactions imex curation IRAK-M mediates Toll-like receptor/IL-1R-induced NFκB activation and cytokine production. IRAK-M mediates Toll-like receptor/IL-1R-induced NFκB activation and cytokine production. EMBO J. (0261-4189) 2013 imex curation Zhou H., Yu M., Fukuda K., Im J., Yao P., Cui W., Bulek K., Zepp J., Wan Y., Kim T.W., Yin W., Ma V., Thomas J., Gu J., Wang J.A., DiCorleto P.E., Fox P.L., Qin J., Li X. lix@ccf.org 10-Mar-2014: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zhou H., Yu M., Fukuda K., Im J., Yao P., Cui W., Bulek K., Zepp J., Wan Y., Kim T.W., Yin W., Ma V., Thomas J., Gu J., Wang J.A., DiCorleto P.E., Fox P.L., Qin J., Li X. lix@ccf.org Accepted 2014-MAR-03 by MARTA EMBO J. (0261-4189) 2013 Only protein-protein interactions imex curation IRAK-M mediates Toll-like receptor/IL-1R-induced NFκB activation and cytokine production. IRAK-M mediates Toll-like receptor/IL-1R-induced NFκB activation and cytokine production. EMBO J. (0261-4189) 2013 imex curation Zhou H., Yu M., Fukuda K., Im J., Yao P., Cui W., Bulek K., Zepp J., Wan Y., Kim T.W., Yin W., Ma V., Thomas J., Gu J., Wang J.A., DiCorleto P.E., Fox P.L., Qin J., Li X. lix@ccf.org 10-Mar-2014: Contacted by IntAct-Help. Only protein-protein interactions mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Zhou H., Yu M., Fukuda K., Im J., Yao P., Cui W., Bulek K., Zepp J., Wan Y., Kim T.W., Yin W., Ma V., Thomas J., Gu J., Wang J.A., DiCorleto P.E., Fox P.L., Qin J., Li X. lix@ccf.org Accepted 2014-MAR-03 by MARTA EMBO J. (0261-4189) 2013 Only protein-protein interactions imex curation IRAK-M mediates Toll-like receptor/IL-1R-induced NFκB activation and cytokine production. IRAK-M mediates Toll-like receptor/IL-1R-induced NFκB activation and cytokine production. EMBO J. (0261-4189) 2013 imex curation Zhou H., Yu M., Fukuda K., Im J., Yao P., Cui W., Bulek K., Zepp J., Wan Y., Kim T.W., Yin W., Ma V., Thomas J., Gu J., Wang J.A., DiCorleto P.E., Fox P.L., Qin J., Li X. lix@ccf.org 10-Mar-2014: Contacted by IntAct-Help. Only protein-protein interactions mouse-bmdm Mus musculus bmdm bone marrow-derived macrophage anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Zhou H., Yu M., Fukuda K., Im J., Yao P., Cui W., Bulek K., Zepp J., Wan Y., Kim T.W., Yin W., Ma V., Thomas J., Gu J., Wang J.A., DiCorleto P.E., Fox P.L., Qin J., Li X. lix@ccf.org Accepted 2014-MAR-03 by MARTA EMBO J. (0261-4189) 2013 Only protein-protein interactions imex curation IRAK-M mediates Toll-like receptor/IL-1R-induced NFκB activation and cytokine production. IRAK-M mediates Toll-like receptor/IL-1R-induced NFκB activation and cytokine production. EMBO J. (0261-4189) 2013 imex curation Zhou H., Yu M., Fukuda K., Im J., Yao P., Cui W., Bulek K., Zepp J., Wan Y., Kim T.W., Yin W., Ma V., Thomas J., Gu J., Wang J.A., DiCorleto P.E., Fox P.L., Qin J., Li X. lix@ccf.org 10-Mar-2014: Contacted by IntAct-Help. Only protein-protein interactions mouse-bmdm Mus musculus bmdm bone marrow-derived macrophage anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zhou H., Yu M., Fukuda K., Im J., Yao P., Cui W., Bulek K., Zepp J., Wan Y., Kim T.W., Yin W., Ma V., Thomas J., Gu J., Wang J.A., DiCorleto P.E., Fox P.L., Qin J., Li X. lix@ccf.org Accepted 2014-MAR-03 by MARTA EMBO J. (0261-4189) 2013 Only protein-protein interactions imex curation m3k3_human Mitogen-activated protein kinase kinase kinase 3 MAP3K3 MAPKKK3 MEKK3 MAPK/ERK kinase kinase 3 protein protein human Homo sapiens Human MDEQEALNSIMNDLVALQMNRRHRMPGYETMKNKDTGHSNRQSDVRIKFEHNGERRIIAFSRPVKYEDVEHKVTTVFGQPLDLHYMNNELSILLKNQDDLDKAIDILDRSSSMKSLRILLLSQDRNHNSSSPHSGVSRQVRIKASQSAGDINTIYQPPEPRSRHLSVSSQNPGRSSPPPGYVPERQQHIARQGSYTSINSEGEFIPETSEQCMLDPLSSAENSLSGSCQSLDRSADSPSFRKSRMSRAQSFPDNRQEYSDRETQLYDKGVKGGTYPRRYHVSVHHKDYSDGRRTFPRIRRHQGNLFTLVPSSRSLSTNGENMGLAVQYLDPRGRLRSADSENALSVQERNVPTKSPSAPINWRRGKLLGQGAFGRVYLCYDVDTGRELASKQVQFDPDSPETSKEVSALECEIQLLKNLQHERIVQYYGCLRDRAEKTLTIFMEYMPGGSVKDQLKAYGALTESVTRKYTRQILEGMSYLHSNMIVHRDIKGANILRDSAGNVKLGDFGASKRLQTICMSGTGMRSVTGTPYWMSPEVISGEGYGRKADVWSLGCTVVEMLTEKPPWAEYEAMAAIFKIATQPTNPQLPSHISEHGRDFLRRIFVEARQRPSAEELLTHHFAQLMY DIP protein Q99759 original sequence version: 164 DIP protein Q99759-1 original sequence version: 173 28129168A57571DD irak4_human Interleukin-1 receptor-associated kinase 4 Renal carcinoma antigen NY-REN-64 IRAK4 protein protein human Homo sapiens Human MNKPITPSTYVRCLNVGLIRKLSDFIDPQEGWKKLAVAIKKPSGDDRYNQFHIRRFEALLQTGKSPTSELLFDWGTTNCTVGDLVDLLIQNEFFAPASLLLPDAVPKTANTLPSKEAITVQQKQMPFCDKDRTLMTPVQNLEQSYMPPDSSSPENKSLEVSDTRFHSFSFYELKNVTNNFDERPISVGGNKMGEGGFGVVYKGYVNNTTVAVKKLAAMVDITTEELKQQFDQEIKVMAKCQHENLVELLGFSSDGDDLCLVYVYMPNGSLLDRLSCLDGTPPLSWHMRCKIAQGAANGINFLHENHHIHRDIKSANILLDEAFTAKISDFGLARASEKFAQTVMTSRIVGTTAYMAPEALRGEITPKSDIYSFGVVLLEIITGLPAVDEHREPQLLLDIKEEIEDEEKTIEDYIDKKMNDADSTSVEAMYSVASQCLHEKKNKRPDIKKVQQLLQEMTAS DIP protein Q9NWZ3 original sequence version: 93 DIP protein Q9NWZ3 original sequence version: 92 DIP protein Q9NWZ3 original sequence version: 144 6C8156ADF25FF81E irak4_mouse Interleukin-1 receptor-associated kinase 4 Irak4 protein protein mouse Mus musculus Mouse MNKPLTPSTYIRNLNVGILRKLSDFIDPQEGWKKLAVAIKKPSGDDRYNQFHIRRFEALLQTGKSPTCELLFDWGTTNCTVGDLVDLLVQIELFAPATLLLPDAVPQTVKSLPPREAATVAQTHGPCQEKDRTSVMPMPKLEHSCEPPDSSSPDNRSVESSDTRFHSFSFHELKSITNNFDEQPASAGGNRMGEGGFGVVYKGCVNNTIVAVKKLGAMVEISTEELKQQFDQEIKVMATCQHENLVELLGFSSDSDNLCLVYAYMPNGSLLDRLSCLDGTPPLSWHTRCKVAQGTANGIRFLHENHHIHRDIKSANILLDKDFTAKISDFGLARASARLAQTVMTSRIVGTTAYMAPEALRGEITPKSDIYSFGVVLLELITGLAAVDENREPQLLLDIKEEIEDEEKTIEDYTDEKMSDADPASVEAMYSAASQCLHEKKNRRPDIAKVQQLLQEMSA DIP protein Q8R4K2 original sequence version: 107 DIP protein Q8R4K2 original sequence version: 98 DIP protein Q8R4K2 original sequence version: 77 FC11AD06983B7AEB irak2_mouse Interleukin-1 receptor-associated kinase-like 2 Irak2 protein protein mouse Mus musculus Mouse MACYIYQLPSWVLDDLCRNIDTLSEWDWMQFASYVITDLTQLRKIKSMERVQGVSITRELLWWWSMRQATVQQLVDLLCHLELYRAAQIVLSWKPVPESTSPLPAFPEAVKPGAVATSRRNLKDEQEKVRPVKPRSLLDTGPIMAGAQRQRPCEMDAPCSLKTDAPDSPQSKYCSTSTSAPKQERLLGLPGDRLFWSEADVVQATEDFDQSHRISEGTFADIYQGQRNGVAFAFKKLREVAGSSPGSMDRFLQAEMQLCLRCCHANVLPLLGFCTGRQFHSLIYPYMANGSLHDRLWAQGNSDMLPWPQRASICSGLLLAVEHLHSLDIIHSNVKSANVLLDQHLNPKLAHPVAHPHPDNKKTKYTVMRTHLFQASAAYLPEHFIRVGQLTKQVDIFSCGIVLAEVLTGIPAMDKDRSPVYLKDLLLSEIPNSTSSVCSRKTSMGKAVVKEICQRHVEKRAGLLPEACEEAWATAVSVCLRRRNASVEEARVSLAGVEEQLRGQLSLPWSRVSEATGSSSNTPEETDDVDNSSLSVPSLVMMASCPGAASSPLFTGHGAAQPSTSGRQEADSSSEACTGPQTPQNATETSWKIEINEAKRRLMENIVLYKEERLDSSELFGP DIP protein Q8CFA1 original sequence version: 74 AD4692A0B6BFE733 myd88_mouse Myeloid differentiation primary response protein MyD88 Myd88 protein protein mouse Mus musculus Mouse MSAGDPRVGSGSLDSFMFSIPLVALNVGVRRRLSLFLNPRTPVAADWTLLAEEMGFEYLEIRELETRPDPTRSLLDAWQGRSGASVGRLLELLALLDREDILKELKSRIEEDCQKYLGKQQNQESEKPLQVARVESSVPQTKELGGITTLDDPLGQTPELFDAFICYCPNDIEFVQEMIRQLEQTDYRLKLCVSDRDVLPGTCVWSIASELIEKRCRRMVVVVSDDYLQSKECDFQTKFALSLSPGVQQKRLIPIKYKAMKKDFPSILRFITICDYTNPCTKSWFWTRLAKALSLP DIP protein P22366 original sequence version: 131 DIP protein P22366 original sequence version: 141 DIP protein P22366-1 original sequence version: 143 DIP protein P22366-1 original sequence version: 144 DIP protein P22366 original sequence version: 122 393A75C3723FFE69 irak3_mouse Interleukin-1 receptor-associated kinase 3 IL-1 receptor-associated kinase M Irak3 Inactive IL-1 receptor-associated kinase 3 protein protein mouse Mus musculus Mouse MAGRCGARGALSPQLLLFDLPPALLGELCGILDSCDGPLGWRGLAERLSNSWLDVRHIEKYVNQGKSGTRELLWSWAQKNKTIGDLLEVLQDMGHQRAIHLIINYGVSWTPSVQTHHELPFPSFPPEVKHACRENDPGPLEPANVTVDNVLVPEHNEKGTLQKTPISFQSILEGTKHFHKDFLIGEGEIFEVYRVDIRNQAYAVKLFKQEKKMQLKKHWKRFLSELEVLLLFRHPHILELAAYFTETEKLCLVYPYMSNGTLFDRLQCTNGTTPLSWHVRISVLIGIAKAIQYLHNTQPCAVICGNVSSANILLDDQLQPKLTDFAAAHFRPNLEQQSSTINMTGGGRKHLWYMPEEYIRQGRLSVKTDVYSFGIVIMEVLTGCKVVLDDPKHVQLRDLLMELMEKRGLDSCLSFLDRKIPPCPRNFSAKLFSLAGRCVATKAKLRPTMDEVLSSLESTQPSLYFAEDPPTSLKSFRCPSPLFLDNVPSIPVEDDENQNNHSVPPKEVLGTDRVTQKTPFECSQSEVTFLGLDRNRGNRGSEADCNVPSSSHEECWSPELVAPSQDLSPTVISLGSSWEVPGHSYGSKPMEKRCSSGLFCSEHEQSKKQ A63E010C9F29D856 traf6_mouse TNF receptor-associated factor 6 RING-type E3 ubiquitin transferase TRAF6 Traf6 E3 ubiquitin-protein ligase TRAF6 protein protein mouse Mus musculus Mouse MSLLNCENSCGSSQSSSDCCAAMAASCSAAVKDDSVSGSASTGNLSSSFMEEIQGYDVEFDPPLESKYECPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVDNEILLENQLFPDNFAKREILSLTVKCPNKGCLQKMELRHLEDHQVHCEFALVNCPQCQRPFQKCQVNTHIIEDCPRRQVSCVNCAVSMAYEEKEIHDQSCPLANIICEYCGTILIREQMPNHYDLDCPTAPIPCTFSVFGCHEKMQRNHLARHLQENTQLHMRLLAQAVHNVNLALRPCDAASPSRGCRPEDPNYEETIKQLESRLVRQDHQIRELTAKMETQSMYVGELKRTIRTLEDKVAEMEAQQCNGIYIWKIGNFGMHLKSQEEERPVVIHSPGFYTGRPGYKLCMRLHLQLPTAQRCANYISLFVHTMQGEYDSHLPWPFQGTIRLTILDQSEALIRQNHEEVMDAKPELLAFQRPTIPRNPKGFGYVTFMHLEALRQGTFIKDDTLLVRCEVSTRFDMGGLRKEGFQPRSTDAGV DIP protein P70196 original sequence version: 139 DIP protein P70196 original sequence version: 128 DIP protein P70196 original sequence version: 144 DIP protein P70196 original sequence version: 83 DIP protein P70196 original sequence version: 98 DIP protein P70196 original sequence version: 104 DIP protein P70196 original sequence version: 112 DIP protein P70196 original sequence version: 163 F8389250425E4E2D irak3-map3k3 3 6 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 10 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level IRAK-M 11 anti tag western anti tag western blot anti tag western western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level association association f2a irak3-irak4 1 8 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 11 anti tag western anti tag western blot anti tag western western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f2a irak3-map3k3-1 4 12 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 6 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 10 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level IRAK-M 11 anti tag western anti tag western blot anti tag western western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association f2b irak4-irak3 5 8 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 11 anti tag western anti tag western blot anti tag western western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f2b irak4-irak3-1 5 8 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level IRAK-M 11 anti tag western anti tag western blot anti tag western western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell Q8K4B2:p.Glu71Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing western blot western blot Immuno blot Immuno blot certain certain sequence position certain certain certain sequence position certain E A Wed May 02 15:41:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu71ala' Tue Jun 30 17:51:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu71Ala' tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f2h irak4-irak3-2 5 8 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level IRAK-M 11 anti tag western anti tag western blot anti tag western western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q8K4B2:p.Gln78Gly mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing western blot western blot Immuno blot Immuno blot certain certain sequence position certain certain certain sequence position certain Q G Wed May 02 19:46:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln78gly' Tue Jun 30 18:29:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln78Gly' physical association physical association f2h irak4-irak3-3 5 8 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level IRAK-M 11 anti tag western anti tag western blot anti tag western western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell Q8K4B2:p.Trp74Ala mutation disrupting mutation disrupting interaction mutation disrupting western blot western blot Immuno blot Immuno blot certain certain sequence position certain certain certain sequence position certain W A Wed May 02 15:38:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp74ala' Tue Jun 30 18:00:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp74Ala' tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f2h irak4-irak3-4 5 8 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level IRAK-M 11 anti tag western anti tag western blot anti tag western western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell Q8K4B2:p.[Glu71Ala;Gln78Gly] mutation disrupting mutation disrupting interaction mutation disrupting western blot western blot Immuno blot Immuno blot certain certain sequence position certain certain certain sequence position certain E A certain certain sequence position certain certain certain sequence position certain Q G Wed May 02 16:50:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu71ala,gln78gly' Tue Jun 30 16:04:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu71Ala;Gln78Gly]' tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f2h irak4-irak3-5 2 7 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level IRAK-M 11 anti tag western anti tag western blot anti tag western western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q8K4B2:p.[Glu71Ala;Gln78Gly] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing western blot western blot Immuno blot Immuno blot certain certain sequence position certain certain certain sequence position certain Q G certain certain sequence position certain certain certain sequence position certain E A Fri Jul 22 10:52:21 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'gln78gly,glu71ala' Wed May 02 20:24:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu71ala,gln78gly' Tue Jun 30 20:20:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu71Ala;Gln78Gly]' physical association physical association f2d irak4-irak3-6 2 7 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level IRAK-M 11 anti tag western anti tag western blot anti tag western western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q8K4B2:p.Glu71Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing western blot western blot Immuno blot Immuno blot certain certain sequence position certain certain certain sequence position certain E A Wed May 02 16:18:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu71ala' Tue Jun 30 18:55:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu71Ala' physical association physical association f2d irak4-irak3-7 2 IRAK-M 11 anti tag western anti tag western blot anti tag western western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell Q8K4B2:p.Gln78Gly mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing western blot western blot Immuno blot Immuno blot certain certain sequence position certain certain certain sequence position certain Q G Wed May 02 15:03:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln78gly' Tue Jun 30 18:10:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln78Gly' tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association f2d irak4-irak3-8 2 7 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level IRAK-M 11 anti tag western anti tag western blot anti tag western western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell Q8K4B2:p.Trp74Ala mutation disrupting mutation disrupting interaction mutation disrupting western blot western blot Immuno blot Immuno blot certain certain sequence position certain certain certain sequence position certain W A Wed May 02 17:24:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp74ala' Tue Jun 30 15:56:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp74Ala' tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f2d irak3-irak4-1 3 IRAK2 9 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 11 anti tag western anti tag western blot anti tag western western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f7e irak4-irak3-9 1 8 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell Q8R4K2:p.Phe25Asp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain F D Wed May 02 20:16:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe25asp' Tue Jun 30 20:03:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe25Asp' 11 anti tag western anti tag western blot anti tag western western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association sf2