IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ GTPase Activity Regulates Kinase Activity and Cellular Phenotypes of Parkinson's Disease-Associated LRRK2. GTPase Activity Regulates Kinase Activity and Cellular Phenotypes of Parkinson's Disease-Associated LRRK2. Hum. Mol. Genet. (0964-6906) 2012 imex curation Biosa A., Trancikova A., Civiero L., Glauser L., Bubacco L., Greggio E., Moore D.J. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease contacted supplier -BD Biosciences about ID for "Hsp90" - immunogen - it common to several Hsp90 variants:--HS90A_HUMAN, HS90B_HUMAN, H90B3_HUMAN, and HS904_HUMAN all at >82% identity. GTP Binding Assay GTPase assay results not included as there has been no further purification of LRRK2 beyond just an initial IP. The IP in Figure 6D for Hsp90 cannot be included due to lack of specificity of the Hsp90 antibody used. darren.moore@epfl.ch 01-March-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom anti tag western anti tag western blot anti tag western Biosa A., Trancikova A., Civiero L., Glauser L., Bubacco L., Greggio E., Moore D.J. Parkinsons - Interactions investigated in the context of Parkinsons disease contacted supplier -BD Biosciences about ID for "Hsp90" - immunogen - it common to several Hsp90 variants:--HS90A_HUMAN, HS90B_HUMAN, H90B3_HUMAN, and HS904_HUMAN all at >82% identity. GTP Binding Assay GTPase assay results not included as there has been no further purification of LRRK2 beyond just an initial IP. The IP in Figure 6D for Hsp90 cannot be included due to lack of specificity of the Hsp90 antibody used. darren.moore@epfl.ch Accepted 2013-JAN-30 by ORCHARD 01-March-2013: Contacted by IntAct-Help. Hum. Mol. Genet. (0964-6906) 2012 Only protein-protein interactions imex curation GTPase Activity Regulates Kinase Activity and Cellular Phenotypes of Parkinson's Disease-Associated LRRK2. GTPase Activity Regulates Kinase Activity and Cellular Phenotypes of Parkinson's Disease-Associated LRRK2. Hum. Mol. Genet. (0964-6906) 2012 imex curation Biosa A., Trancikova A., Civiero L., Glauser L., Bubacco L., Greggio E., Moore D.J. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease contacted supplier -BD Biosciences about ID for "Hsp90" - immunogen - it common to several Hsp90 variants:--HS90A_HUMAN, HS90B_HUMAN, H90B3_HUMAN, and HS904_HUMAN all at >82% identity. GTP Binding Assay GTPase assay results not included as there has been no further purification of LRRK2 beyond just an initial IP. The IP in Figure 6D for Hsp90 cannot be included due to lack of specificity of the Hsp90 antibody used. darren.moore@epfl.ch 01-March-2013: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column anti tag western anti tag western blot anti tag western Biosa A., Trancikova A., Civiero L., Glauser L., Bubacco L., Greggio E., Moore D.J. Parkinsons - Interactions investigated in the context of Parkinsons disease contacted supplier -BD Biosciences about ID for "Hsp90" - immunogen - it common to several Hsp90 variants:--HS90A_HUMAN, HS90B_HUMAN, H90B3_HUMAN, and HS904_HUMAN all at >82% identity. GTP Binding Assay GTPase assay results not included as there has been no further purification of LRRK2 beyond just an initial IP. The IP in Figure 6D for Hsp90 cannot be included due to lack of specificity of the Hsp90 antibody used. darren.moore@epfl.ch Accepted 2013-JAN-30 by ORCHARD 01-March-2013: Contacted by IntAct-Help. Hum. Mol. Genet. (0964-6906) 2012 Only protein-protein interactions imex curation GTPase Activity Regulates Kinase Activity and Cellular Phenotypes of Parkinson's Disease-Associated LRRK2. GTPase Activity Regulates Kinase Activity and Cellular Phenotypes of Parkinson's Disease-Associated LRRK2. Hum. Mol. Genet. (0964-6906) 2012 imex curation Biosa A., Trancikova A., Civiero L., Glauser L., Bubacco L., Greggio E., Moore D.J. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease contacted supplier -BD Biosciences about ID for "Hsp90" - immunogen - it common to several Hsp90 variants:--HS90A_HUMAN, HS90B_HUMAN, H90B3_HUMAN, and HS904_HUMAN all at >82% identity. GTP Binding Assay GTPase assay results not included as there has been no further purification of LRRK2 beyond just an initial IP. The IP in Figure 6D for Hsp90 cannot be included due to lack of specificity of the Hsp90 antibody used. darren.moore@epfl.ch 01-March-2013: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Biosa A., Trancikova A., Civiero L., Glauser L., Bubacco L., Greggio E., Moore D.J. Parkinsons - Interactions investigated in the context of Parkinsons disease contacted supplier -BD Biosciences about ID for "Hsp90" - immunogen - it common to several Hsp90 variants:--HS90A_HUMAN, HS90B_HUMAN, H90B3_HUMAN, and HS904_HUMAN all at >82% identity. GTP Binding Assay GTPase assay results not included as there has been no further purification of LRRK2 beyond just an initial IP. The IP in Figure 6D for Hsp90 cannot be included due to lack of specificity of the Hsp90 antibody used. darren.moore@epfl.ch Accepted 2013-JAN-30 by ORCHARD 01-March-2013: Contacted by IntAct-Help. Hum. Mol. Genet. (0964-6906) 2012 Only protein-protein interactions imex curation GTPase Activity Regulates Kinase Activity and Cellular Phenotypes of Parkinson's Disease-Associated LRRK2. GTPase Activity Regulates Kinase Activity and Cellular Phenotypes of Parkinson's Disease-Associated LRRK2. Hum. Mol. Genet. (0964-6906) 2012 imex curation Biosa A., Trancikova A., Civiero L., Glauser L., Bubacco L., Greggio E., Moore D.J. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease contacted supplier -BD Biosciences about ID for "Hsp90" - immunogen - it common to several Hsp90 variants:--HS90A_HUMAN, HS90B_HUMAN, H90B3_HUMAN, and HS904_HUMAN all at >82% identity. GTP Binding Assay GTPase assay results not included as there has been no further purification of LRRK2 beyond just an initial IP. The IP in Figure 6D for Hsp90 cannot be included due to lack of specificity of the Hsp90 antibody used. darren.moore@epfl.ch 01-March-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Biosa A., Trancikova A., Civiero L., Glauser L., Bubacco L., Greggio E., Moore D.J. Parkinsons - Interactions investigated in the context of Parkinsons disease contacted supplier -BD Biosciences about ID for "Hsp90" - immunogen - it common to several Hsp90 variants:--HS90A_HUMAN, HS90B_HUMAN, H90B3_HUMAN, and HS904_HUMAN all at >82% identity. GTP Binding Assay GTPase assay results not included as there has been no further purification of LRRK2 beyond just an initial IP. The IP in Figure 6D for Hsp90 cannot be included due to lack of specificity of the Hsp90 antibody used. darren.moore@epfl.ch Accepted 2013-JAN-30 by ORCHARD 01-March-2013: Contacted by IntAct-Help. Hum. Mol. Genet. (0964-6906) 2012 Only protein-protein interactions imex curation GTPase Activity Regulates Kinase Activity and Cellular Phenotypes of Parkinson's Disease-Associated LRRK2. GTPase Activity Regulates Kinase Activity and Cellular Phenotypes of Parkinson's Disease-Associated LRRK2. Hum. Mol. Genet. (0964-6906) 2012 imex curation Biosa A., Trancikova A., Civiero L., Glauser L., Bubacco L., Greggio E., Moore D.J. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease contacted supplier -BD Biosciences about ID for "Hsp90" - immunogen - it common to several Hsp90 variants:--HS90A_HUMAN, HS90B_HUMAN, H90B3_HUMAN, and HS904_HUMAN all at >82% identity. GTP Binding Assay GTPase assay results not included as there has been no further purification of LRRK2 beyond just an initial IP. The IP in Figure 6D for Hsp90 cannot be included due to lack of specificity of the Hsp90 antibody used. darren.moore@epfl.ch 01-March-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Biosa A., Trancikova A., Civiero L., Glauser L., Bubacco L., Greggio E., Moore D.J. Parkinsons - Interactions investigated in the context of Parkinsons disease contacted supplier -BD Biosciences about ID for "Hsp90" - immunogen - it common to several Hsp90 variants:--HS90A_HUMAN, HS90B_HUMAN, H90B3_HUMAN, and HS904_HUMAN all at >82% identity. GTP Binding Assay GTPase assay results not included as there has been no further purification of LRRK2 beyond just an initial IP. The IP in Figure 6D for Hsp90 cannot be included due to lack of specificity of the Hsp90 antibody used. darren.moore@epfl.ch Accepted 2013-JAN-30 by ORCHARD 01-March-2013: Contacted by IntAct-Help. Hum. Mol. Genet. (0964-6906) 2012 Only protein-protein interactions imex curation gtp guanosine triphosphate - GTP 5'-GTP GTP Guanosine 5'-triphosphate guanosine 5'-triphosphoric acid guanosine triphosphate GUANOSINE-5'-TRIPHOSPHATE H4gtp guanosine 5'-(tetrahydrogen triphosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2 InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2 lrrk2_human Leucine-rich repeat serine/threonine-protein kinase 2 LRRK2 PARK8 Dardarin protein protein human Homo sapiens Human MASGSCQGCEEDEETLKKLIVRLNNVQEGKQIETLVQILEDLLVFTYSERASKLFQGKNIHVPLLIVLDSYMRVASVQQVGWSLLCKLIEVCPGTMQSLMGPQDVGNDWEVLGVHQLILKMLTVHNASVNLSVIGLKTLDLLLTSGKITLLILDEESDIFMLIFDAMHSFPANDEVQKLGCKALHVLFERVSEEQLTEFVENKDYMILLSALTNFKDEEEIVLHVLHCLHSLAIPCNNVEVLMSGNVRCYNIVVEAMKAFPMSERIQEVSCCLLHRLTLGNFFNILVLNEVHEFVVKAVQQYPENAALQISALSCLALLTETIFLNQDLEEKNENQENDDEGEEDKLFWLEACYKALTWHRKNKHVQEAACWALNNLLMYQNSLHEKIGDEDGHFPAHREVMLSMLMHSSSKEVFQASANALSTLLEQNVNFRKILLSKGIHLNVLELMQKHIHSPEVAESGCKMLNHLFEGSNTSLDIMAAVVPKILTVMKRHETSLPVQLEALRAILHFIVPGMPEESREDTEFHHKLNMVKKQCFKNDIHKLVLAALNRFIGNPGIQKCGLKVISSIVHFPDALEMLSLEGAMDSVLHTLQMYPDDQEIQCLGLSLIGYLITKKNVFIGTGHLLAKILVSSLYRFKDVAEIQTKGFQTILAILKLSASFSKLLVHHSFDLVIFHQMSSNIMEQKDQQFLNLCCKCFAKVAMDDYLKNVMLERACDQNNSIMVECLLLLGADANQAKEGSSLICQVCEKESSPKLVELLLNSGSREQDVRKALTISIGKGDSQIISLLLRRLALDVANNSICLGGFCIGKVEPSWLGPLFPDKTSNLRKQTNIASTLARMVIRYQMKSAVEEGTASGSDGNFSEDVLSKFDEWTFIPDSSMDSVFAQSDDLDSEGSEGSFLVKKKSNSISVGEFYRDAVLQRCSPNLQRHSNSLGPIFDHEDLLKRKRKILSSDDSLRSSKLQSHMRHSDSISSLASEREYITSLDLSANELRDIDALSQKCCISVHLEHLEKLELHQNALTSFPQQLCETLKSLTHLDLHSNKFTSFPSYLLKMSCIANLDVSRNDIGPSVVLDPTVKCPTLKQFNLSYNQLSFVPENLTDVVEKLEQLILEGNKISGICSPLRLKELKILNLSKNHISSLSENFLEACPKVESFSARMNFLAAMPFLPPSMTILKLSQNKFSCIPEAILNLPHLRSLDMSSNDIQYLPGPAHWKSLNLRELLFSHNQISILDLSEKAYLWSRVEKLHLSHNKLKEIPPEIGCLENLTSLDVSYNLELRSFPNEMGKLSKIWDLPLDELHLNFDFKHIGCKAKDIIRFLQQRLKKAVPYNRMKLMIVGNTGSGKTTLLQQLMKTKKSDLGMQSATVGIDVKDWPIQIRDKRKRDLVLNVWDFAGREEFYSTHPHFMTQRALYLAVYDLSKGQAEVDAMKPWLFNIKARASSSPVILVGTHLDVSDEKQRKACMSKITKELLNKRGFPAIRDYHFVNATEESDALAKLRKTIINESLNFKIRDQLVVGQLIPDCYVELEKIILSERKNVPIEFPVIDRKRLLQLVRENQLQLDENELPHAVHFLNESGVLLHFQDPALQLSDLYFVEPKWLCKIMAQILTVKVEGCPKHPKGIISRRDVEKFLSKKRKFPKNYMSQYFKLLEKFQIALPIGEEYLLVPSSLSDHRPVIELPHCENSEIIIRLYEMPYFPMGFWSRLINRLLEISPYMLSGRERALRPNRMYWRQGIYLNWSPEAYCLVGSEVLDNHPESFLKITVPSCRKGCILLGQVVDHIDSLMEEWFPGLLEIDICGEGETLLKKWALYSFNDGEEHQKILLDDLMKKAEEGDLLVNPDQPRLTIPISQIAPDLILADLPRNIMLNNDELEFEQAPEFLLGDGSFGSVYRAAYEGEEVAVKIFNKHTSLRLLRQELVVLCHLHHPSLISLLAAGIRPRMLVMELASKGSLDRLLQQDKASLTRTLQHRIALHVADGLRYLHSAMIIYRDLKPHNVLLFTLYPNAAIIAKIADYGIAQYCCRMGIKTSEGTPGFRAPEVARGNVIYNQQADVYSFGLLLYDILTTGGRIVEGLKFPNEFDELEIQGKLPDPVKEYGCAPWPMVEKLIKQCLKENPQERPTSAQVFDILNSAELVCLTRRILLPKNVIVECMVATHHNSRNASIWLGCGHTDRGQLSFLDLNTEGYTSEEVADSRILCLALVHLPVEKESWIVSGTQSGTLLVINTEDGKKRHTLEKMTDSVTCLYCNSFSKQSKQKNFLLVGTADGKLAIFEDKTVKLKGAAPLKILNIGNVSTPLMCLSESTNSTERNVMWGGCGTKIFSFSNDFTIQKLIETRTSQLFSYAAFSDSNIITVVVDTALYIAKQNSPVVEVWDKKTEKLCGLIDCVHFLREVMVKENKESKHKMSYSGRVKTLCLQKNTALWIGTGGGHILLLDLSTRRLIRVIYNFCNSVRVMMTAQLGSLKNVMLVLGYNRKNTEGTQKQKEIQSCLTVWDINLPHEVQNLEKHIEVRKELAEKMRRTSVE DIP protein Q5S007 original sequence version: 36 DIP protein Q5S007 original sequence version: 68 DIP protein Q5S007 original sequence version: 64 26142A0CECBBC3F4 moes_human Moesin Membrane-organizing extension spike protein MSN protein protein human Homo sapiens Human MPKTISVRVTTMDAELEFAIQPNTTGKQLFDQVVKTIGLREVWFFGLQYQDTKGFSTWLKLNKKVTAQDVRKESPLLFKFRAKFYPEDVSEELIQDITQRLFFLQVKEGILNDDIYCPPETAVLLASYAVQSKYGDFNKEVHKSGYLAGDKLLPQRVLEQHKLNKDQWEERIQVWHEEHRGMLREDAVLEYLKIAQDLEMYGVNYFSIKNKKGSELWLGVDALGLNIYEQNDRLTPKIGFPWSEIRNISFNDKKFVIKPIDKKAPDFVFYAPRLRINKRILALCMGNHELYMRRRKPDTIEVQQMKAQAREEKHQKQMERAMLENEKKKREMAEKEKEKIEREKEELMERLKQIEEQTKKAQQELEEQTRRALELEQERKRAQSEAEKLAKERQEAEEAKEALLQASRDQKKTQEQLALEMAELTARISQLEMARQKKESEAVEWQQKAQMVQEDLEKTRAELKTAMSTPHVAEPAENEQDEQDENGAEASADLRADAMAKDRSEEERTTEAEKNERVQKHLKALTSELANARDESKKTANDMIHAENMRLGRDKYKTLRQIRQGNTKQRIDEFESM DIP protein P26038 original sequence version: 168 865A6C5CB14AE586 gtp-lrrk2-5 1 6 unspecified role unspecified role bait bait chemical synthesis Chemical synthesis (Chemical synthesis) 7 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q5S007:p.Lys1347Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain K A Wed May 02 18:48:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys1347ala' Tue Jun 30 19:00:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys1347Ala' Q5S007:p.Thr1348Asn mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain T N Wed May 02 17:42:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr1348asn' Tue Jun 30 18:45:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr1348Asn' Q5S007:p.[Thr1343Val;Arg1398Leu;Asp1994Ala] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain R L certain certain sequence position certain certain certain sequence position certain D A certain certain sequence position certain certain certain sequence position certain T V Fri Jul 22 10:52:19 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'arg1398leu,asp1994ala,thr1343val' Wed May 02 17:08:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr1343val,arg1398leu,asp1994ala' Tue Jun 30 16:48:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Thr1343Val;Arg1398Leu;Asp1994Ala]' Q5S007:p.[Thr1348Asn;Gly2019Ser] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain G S certain certain sequence position certain certain certain sequence position certain T N Fri Jul 22 10:50:03 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'gly2019ser,thr1348asn' Wed May 02 17:09:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr1348asn,gly2019ser' Tue Jun 30 17:53:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Thr1348Asn;Gly2019Ser]' Q5S007:p.[Arg1398Leu;Asp1994Ala] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain D A certain certain sequence position certain certain certain sequence position certain R L Fri Jul 22 11:02:16 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'arg1398leu,asp1994ala,thr1343val' Wed May 02 15:19:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg1398leu,asp1994ala' Tue Jun 30 19:22:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg1398Leu;Asp1994Ala]' human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. physical association physical association Table 1, figure 3A lrrk2-1 5 7 putative self putative self putative self putative self Q5S007:p.[Asp1994Ala;Gly2019Ser] mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain G S certain certain sequence position certain certain certain sequence position certain D A Fri Jul 22 10:56:47 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'gly2019ser,asp1994ala' Wed May 02 18:42:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp1994ala,gly2019ser' Tue Jun 30 19:10:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asp1994Ala;Gly2019Ser]' Q5S007:p.Lys1347Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain K A Wed May 02 19:13:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys1347ala' Tue Jun 30 19:50:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys1347Ala' region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q5S007:p.[Thr1348Asn;Gly2019Ser] mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain G S certain certain sequence position certain certain certain sequence position certain T N Fri Jul 22 10:56:13 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'gly2019ser,thr1348asn' Wed May 02 19:39:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr1348asn,gly2019ser' Tue Jun 30 20:03:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Thr1348Asn;Gly2019Ser]' Q5S007:p.Asp1994Asn mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain D N Wed May 02 17:00:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp1994asn' Tue Jun 30 18:18:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp1994Asn' Q5S007:p.Thr1348Asn mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain T N Wed May 02 14:32:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr1348asn' Tue Jun 30 18:18:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr1348Asn' Q5S007:p.Gly2019Ser mutation increasing rate mutation increasing interaction rate mutation increasing rate certain certain sequence position certain certain certain sequence position certain G S Wed May 02 18:15:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly2019ser' Tue Jun 30 17:58:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly2019Ser' Q5S007:p.Arg1398Leu mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain R L Wed May 02 18:17:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg1398leu' Tue Jun 30 18:15:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg1398Leu' Q5S007:p.[Thr1343Val;Arg1398Leu;Asp1994Ala] mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain T V certain certain sequence position certain certain certain sequence position certain R L certain certain sequence position certain certain certain sequence position certain D A Wed May 02 15:48:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr1343val,arg1398leu,asp1994ala' Tue Jun 30 16:23:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Thr1343Val;Arg1398Leu;Asp1994Ala]' Q5S007:p.Asp1994Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain D A Wed May 02 16:44:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp1994ala' Tue Jun 30 19:12:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp1994Ala' human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. phosphorylation phosphorylation reaction phosphorylation true Fig 4A/B lrrk2-msn-5 4 7 enzyme enzyme neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q5S007:p.Arg1398Leu mutation increasing rate mutation increasing interaction rate mutation increasing rate certain certain sequence position certain certain certain sequence position certain R L Wed May 02 18:32:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg1398leu' Tue Jun 30 19:13:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg1398Leu' Q5S007:p.[Thr1343Val;Arg1398Leu] mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain R L certain certain sequence position certain certain certain sequence position certain T V Fri Jul 22 11:14:04 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'arg1398leu,thr1343val' Wed May 02 17:49:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr1343val,arg1398leu' Tue Jun 30 20:20:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Thr1343Val;Arg1398Leu]' Q5S007:p.Gly2019Ser mutation increasing rate mutation increasing interaction rate mutation increasing rate certain certain sequence position certain certain certain sequence position certain G S Wed May 02 17:02:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly2019ser' Tue Jun 30 19:42:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly2019Ser' Q5S007:p.Thr1348Asn mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain T N Wed May 02 17:49:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr1348asn' Tue Jun 30 15:37:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr1348Asn' human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 8 enzyme target enzyme target substrate substrate neutral component neutral component region_lrrktide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) phosphorylation phosphorylation reaction phosphorylation 5 I have not amalgamated this interaction with Fig4C for clarity also as I have no idea the effect of the additional masses of mutations on this interaction. -not shown. GTP and GppCp (methyleneguanosine 5′-triphosphate) added to cell lysates immediately prior to to incubation with anti-FLAG-agarose.. GDP added immediately prior to to incubation with anti-FLAG-agarose. lrrk2-msn-5 4 7 enzyme enzyme neutral component neutral component Q5S007:p.Asp1994Asn mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain D N Wed May 02 15:42:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp1994asn' Tue Jun 30 15:58:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp1994Asn' Q5S007:p.[Thr1343Val;Arg1398Leu] mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain R L certain certain sequence position certain certain certain sequence position certain T V Fri Jul 22 10:53:27 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'arg1398leu,thr1343val' Wed May 02 16:07:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr1343val,arg1398leu' Tue Jun 30 20:22:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Thr1343Val;Arg1398Leu]' region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q5S007:p.[Thr1343Val;Arg1398Leu;Asp1994Ala] mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain T V certain certain sequence position certain certain certain sequence position certain R L certain certain sequence position certain certain certain sequence position certain D A Wed May 02 17:17:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr1343val,arg1398leu,asp1994ala' Tue Jun 30 15:43:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Thr1343Val;Arg1398Leu;Asp1994Ala]' Q5S007:p.[Asp1994Ala;Gly2019Ser] mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain G S certain certain sequence position certain certain certain sequence position certain D A Fri Jul 22 11:15:37 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'gly2019ser,asp1994ala' Wed May 02 14:43:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp1994ala,gly2019ser' Tue Jun 30 18:37:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asp1994Ala;Gly2019Ser]' Q5S007:p.Thr1348Asn mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain T N Wed May 02 15:01:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr1348asn' Tue Jun 30 20:13:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr1348Asn' Q5S007:p.[Arg1398Leu;Asp1994Ala] mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain D A certain certain sequence position certain certain certain sequence position certain R L Fri Jul 22 11:01:41 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'asp1994ala,arg1398leu' Wed May 02 16:02:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg1398leu,asp1994ala' Tue Jun 30 16:28:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg1398Leu;Asp1994Ala]' Q5S007:p.Lys1906Met mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain K M Wed May 02 15:11:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys1906met' Tue Jun 30 16:32:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys1906Met' Q5S007:p.Gly2019Ser mutation increasing rate mutation increasing interaction rate mutation increasing rate certain certain sequence position certain certain certain sequence position certain G S Wed May 02 15:58:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly2019ser' Tue Jun 30 16:41:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly2019Ser' Q5S007:p.Lys1347Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain K A Wed May 02 17:23:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys1347ala' Tue Jun 30 19:21:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys1347Ala' Q5S007:p.[Thr1348Asn;Gly2019Ser] mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain G S certain certain sequence position certain certain certain sequence position certain T N Fri Jul 22 11:00:34 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'gly2019ser,thr1348asn' Wed May 02 15:23:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr1348asn,gly2019ser' Tue Jun 30 17:27:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Thr1348Asn;Gly2019Ser]' human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 8 enzyme target enzyme target substrate substrate neutral component neutral component region_lrrktide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) phosphorylation phosphorylation reaction phosphorylation Fig 4C lrrk2-lrrk2-41 2 7 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 6 A/B The R1398L, R1398L/T1343V and R1398Q/T1343G mutants do not obviously influence the hydrodynamic volume of LRRK2 under native conditions relative to WT protein. lrrk2-lrrk2-43 3 7 unspecified role unspecified role prey prey Q5S007:p.Asp1994Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain D A Wed May 02 14:55:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp1994ala' Tue Jun 30 18:33:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp1994Ala' Q5S007:p.Thr1348Asn mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain T N Wed May 02 17:38:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr1348asn' Tue Jun 30 17:18:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr1348Asn' region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q5S007:p.Lys1347Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain K A Wed May 02 19:17:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys1347ala' Tue Jun 30 17:55:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys1347Ala' Q5S007:p.[Asp1994Ala;Gly2019Ser] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain G S certain certain sequence position certain certain certain sequence position certain D A Fri Jul 22 10:54:42 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'gly2019ser,asp1994ala' Wed May 02 17:11:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp1994ala,gly2019ser' Tue Jun 30 18:54:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asp1994Ala;Gly2019Ser]' 7 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 6C