IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ LRRK2 interactions with α-synuclein in Parkinson's disease brains and in cell models. LRRK2 interactions with α-synuclein in Parkinson's disease brains and in cell models. J. Mol. Med. (0946-2716) 2012 imex curation Guerreiro P.S., Huang Y., Gysbers A., Cheng D., Gai W.P., Outeiro T.F., Halliday G.M. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease The authors confirm that the a-synuclein plasmid used was Human in origin. touteiro@gmail.com, g.halliday@neura.edu.au 01-March-2013: Contacted by IntAct-Help. Only protein-protein interactions mouse-brain Mus musculus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Guerreiro P.S., Huang Y., Gysbers A., Cheng D., Gai W.P., Outeiro T.F., Halliday G.M. Parkinsons - Interactions investigated in the context of Parkinsons disease The authors confirm that the a-synuclein plasmid used was Human in origin. touteiro@gmail.com, g.halliday@neura.edu.au Accepted 2013-FEB-06 by PPORRAS 01-March-2013: Contacted by IntAct-Help. J. Mol. Med. (0946-2716) 2012 Only protein-protein interactions imex curation LRRK2 interactions with α-synuclein in Parkinson's disease brains and in cell models. LRRK2 interactions with α-synuclein in Parkinson's disease brains and in cell models. J. Mol. Med. (0946-2716) 2012 imex curation Guerreiro P.S., Huang Y., Gysbers A., Cheng D., Gai W.P., Outeiro T.F., Halliday G.M. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease The authors confirm that the a-synuclein plasmid used was Human in origin. touteiro@gmail.com, g.halliday@neura.edu.au 01-March-2013: Contacted by IntAct-Help. Only protein-protein interactions human-neuron Human neurons neuron Neuron [Neuronal cell; Neural cell confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Guerreiro P.S., Huang Y., Gysbers A., Cheng D., Gai W.P., Outeiro T.F., Halliday G.M. Parkinsons - Interactions investigated in the context of Parkinsons disease Neurons from the Substantia Nigra (midbrain). The authors confirm that the a-synuclein plasmid used was Human in origin. touteiro@gmail.com, g.halliday@neura.edu.au Accepted 2013-FEB-06 by PPORRAS 01-March-2013: Contacted by IntAct-Help. J. Mol. Med. (0946-2716) 2012 Only protein-protein interactions imex curation LRRK2 interactions with α-synuclein in Parkinson's disease brains and in cell models. LRRK2 interactions with α-synuclein in Parkinson's disease brains and in cell models. J. Mol. Med. (0946-2716) 2012 imex curation Guerreiro P.S., Huang Y., Gysbers A., Cheng D., Gai W.P., Outeiro T.F., Halliday G.M. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease The authors confirm that the a-synuclein plasmid used was Human in origin. touteiro@gmail.com, g.halliday@neura.edu.au 01-March-2013: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Guerreiro P.S., Huang Y., Gysbers A., Cheng D., Gai W.P., Outeiro T.F., Halliday G.M. Parkinsons - Interactions investigated in the context of Parkinsons disease The authors confirm that the a-synuclein plasmid used was Human in origin. touteiro@gmail.com, g.halliday@neura.edu.au Accepted 2013-FEB-06 by PPORRAS 01-March-2013: Contacted by IntAct-Help. J. Mol. Med. (0946-2716) 2012 Only protein-protein interactions imex curation LRRK2 interactions with α-synuclein in Parkinson's disease brains and in cell models. LRRK2 interactions with α-synuclein in Parkinson's disease brains and in cell models. J. Mol. Med. (0946-2716) 2012 imex curation Guerreiro P.S., Huang Y., Gysbers A., Cheng D., Gai W.P., Outeiro T.F., Halliday G.M. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease The authors confirm that the a-synuclein plasmid used was Human in origin. touteiro@gmail.com, g.halliday@neura.edu.au 01-March-2013: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Guerreiro P.S., Huang Y., Gysbers A., Cheng D., Gai W.P., Outeiro T.F., Halliday G.M. Parkinsons - Interactions investigated in the context of Parkinsons disease The authors confirm that the a-synuclein plasmid used was Human in origin. touteiro@gmail.com, g.halliday@neura.edu.au Accepted 2013-FEB-06 by PPORRAS 01-March-2013: Contacted by IntAct-Help. J. Mol. Med. (0946-2716) 2012 Only protein-protein interactions imex curation LRRK2 interactions with α-synuclein in Parkinson's disease brains and in cell models. LRRK2 interactions with α-synuclein in Parkinson's disease brains and in cell models. J. Mol. Med. (0946-2716) 2012 imex curation Guerreiro P.S., Huang Y., Gysbers A., Cheng D., Gai W.P., Outeiro T.F., Halliday G.M. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease The authors confirm that the a-synuclein plasmid used was Human in origin. touteiro@gmail.com, g.halliday@neura.edu.au 01-March-2013: Contacted by IntAct-Help. Only protein-protein interactions human-h4 Homo sapiens neurogioma cell line h4 Human neuroglioma confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Guerreiro P.S., Huang Y., Gysbers A., Cheng D., Gai W.P., Outeiro T.F., Halliday G.M. Parkinsons - Interactions investigated in the context of Parkinsons disease The authors confirm that the a-synuclein plasmid used was Human in origin. touteiro@gmail.com, g.halliday@neura.edu.au Accepted 2013-FEB-06 by PPORRAS 01-March-2013: Contacted by IntAct-Help. J. Mol. Med. (0946-2716) 2012 Only protein-protein interactions imex curation LRRK2 interactions with α-synuclein in Parkinson's disease brains and in cell models. LRRK2 interactions with α-synuclein in Parkinson's disease brains and in cell models. J. Mol. Med. (0946-2716) 2012 imex curation Guerreiro P.S., Huang Y., Gysbers A., Cheng D., Gai W.P., Outeiro T.F., Halliday G.M. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease The authors confirm that the a-synuclein plasmid used was Human in origin. touteiro@gmail.com, g.halliday@neura.edu.au 01-March-2013: Contacted by IntAct-Help. Only protein-protein interactions human-brain Homo sapiens brain brain Brain [cerebrum] confocal microscopy confocal microscopy immunohistochemistry immunohistochemistry Guerreiro P.S., Huang Y., Gysbers A., Cheng D., Gai W.P., Outeiro T.F., Halliday G.M. Parkinsons - Interactions investigated in the context of Parkinsons disease Tissue sections of the midbrain and anterior cingulate cortex from PD brain samples. Single neurons in the substantia nigra are shown. The authors confirm that the a-synuclein plasmid used was Human in origin. touteiro@gmail.com, g.halliday@neura.edu.au Accepted 2013-FEB-06 by PPORRAS 01-March-2013: Contacted by IntAct-Help. J. Mol. Med. (0946-2716) 2012 Only protein-protein interactions imex curation syua_mouse Alpha-synuclein Snca Syn Non-A beta component of AD amyloid Non-A4 component of amyloid precursor protein protein mouse Mus musculus Mouse MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVTTVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGNIAAATGFVKKDQMGKGEEGYPQEGILEDMPVDPGSEAYEMPSEEGYQDYEPEA 1FFD19D7E15E636C lrrk2_human Leucine-rich repeat serine/threonine-protein kinase 2 LRRK2 PARK8 Dardarin protein protein human Homo sapiens Human MASGSCQGCEEDEETLKKLIVRLNNVQEGKQIETLVQILEDLLVFTYSERASKLFQGKNIHVPLLIVLDSYMRVASVQQVGWSLLCKLIEVCPGTMQSLMGPQDVGNDWEVLGVHQLILKMLTVHNASVNLSVIGLKTLDLLLTSGKITLLILDEESDIFMLIFDAMHSFPANDEVQKLGCKALHVLFERVSEEQLTEFVENKDYMILLSALTNFKDEEEIVLHVLHCLHSLAIPCNNVEVLMSGNVRCYNIVVEAMKAFPMSERIQEVSCCLLHRLTLGNFFNILVLNEVHEFVVKAVQQYPENAALQISALSCLALLTETIFLNQDLEEKNENQENDDEGEEDKLFWLEACYKALTWHRKNKHVQEAACWALNNLLMYQNSLHEKIGDEDGHFPAHREVMLSMLMHSSSKEVFQASANALSTLLEQNVNFRKILLSKGIHLNVLELMQKHIHSPEVAESGCKMLNHLFEGSNTSLDIMAAVVPKILTVMKRHETSLPVQLEALRAILHFIVPGMPEESREDTEFHHKLNMVKKQCFKNDIHKLVLAALNRFIGNPGIQKCGLKVISSIVHFPDALEMLSLEGAMDSVLHTLQMYPDDQEIQCLGLSLIGYLITKKNVFIGTGHLLAKILVSSLYRFKDVAEIQTKGFQTILAILKLSASFSKLLVHHSFDLVIFHQMSSNIMEQKDQQFLNLCCKCFAKVAMDDYLKNVMLERACDQNNSIMVECLLLLGADANQAKEGSSLICQVCEKESSPKLVELLLNSGSREQDVRKALTISIGKGDSQIISLLLRRLALDVANNSICLGGFCIGKVEPSWLGPLFPDKTSNLRKQTNIASTLARMVIRYQMKSAVEEGTASGSDGNFSEDVLSKFDEWTFIPDSSMDSVFAQSDDLDSEGSEGSFLVKKKSNSISVGEFYRDAVLQRCSPNLQRHSNSLGPIFDHEDLLKRKRKILSSDDSLRSSKLQSHMRHSDSISSLASEREYITSLDLSANELRDIDALSQKCCISVHLEHLEKLELHQNALTSFPQQLCETLKSLTHLDLHSNKFTSFPSYLLKMSCIANLDVSRNDIGPSVVLDPTVKCPTLKQFNLSYNQLSFVPENLTDVVEKLEQLILEGNKISGICSPLRLKELKILNLSKNHISSLSENFLEACPKVESFSARMNFLAAMPFLPPSMTILKLSQNKFSCIPEAILNLPHLRSLDMSSNDIQYLPGPAHWKSLNLRELLFSHNQISILDLSEKAYLWSRVEKLHLSHNKLKEIPPEIGCLENLTSLDVSYNLELRSFPNEMGKLSKIWDLPLDELHLNFDFKHIGCKAKDIIRFLQQRLKKAVPYNRMKLMIVGNTGSGKTTLLQQLMKTKKSDLGMQSATVGIDVKDWPIQIRDKRKRDLVLNVWDFAGREEFYSTHPHFMTQRALYLAVYDLSKGQAEVDAMKPWLFNIKARASSSPVILVGTHLDVSDEKQRKACMSKITKELLNKRGFPAIRDYHFVNATEESDALAKLRKTIINESLNFKIRDQLVVGQLIPDCYVELEKIILSERKNVPIEFPVIDRKRLLQLVRENQLQLDENELPHAVHFLNESGVLLHFQDPALQLSDLYFVEPKWLCKIMAQILTVKVEGCPKHPKGIISRRDVEKFLSKKRKFPKNYMSQYFKLLEKFQIALPIGEEYLLVPSSLSDHRPVIELPHCENSEIIIRLYEMPYFPMGFWSRLINRLLEISPYMLSGRERALRPNRMYWRQGIYLNWSPEAYCLVGSEVLDNHPESFLKITVPSCRKGCILLGQVVDHIDSLMEEWFPGLLEIDICGEGETLLKKWALYSFNDGEEHQKILLDDLMKKAEEGDLLVNPDQPRLTIPISQIAPDLILADLPRNIMLNNDELEFEQAPEFLLGDGSFGSVYRAAYEGEEVAVKIFNKHTSLRLLRQELVVLCHLHHPSLISLLAAGIRPRMLVMELASKGSLDRLLQQDKASLTRTLQHRIALHVADGLRYLHSAMIIYRDLKPHNVLLFTLYPNAAIIAKIADYGIAQYCCRMGIKTSEGTPGFRAPEVARGNVIYNQQADVYSFGLLLYDILTTGGRIVEGLKFPNEFDELEIQGKLPDPVKEYGCAPWPMVEKLIKQCLKENPQERPTSAQVFDILNSAELVCLTRRILLPKNVIVECMVATHHNSRNASIWLGCGHTDRGQLSFLDLNTEGYTSEEVADSRILCLALVHLPVEKESWIVSGTQSGTLLVINTEDGKKRHTLEKMTDSVTCLYCNSFSKQSKQKNFLLVGTADGKLAIFEDKTVKLKGAAPLKILNIGNVSTPLMCLSESTNSTERNVMWGGCGTKIFSFSNDFTIQKLIETRTSQLFSYAAFSDSNIITVVVDTALYIAKQNSPVVEVWDKKTEKLCGLIDCVHFLREVMVKENKESKHKMSYSGRVKTLCLQKNTALWIGTGGGHILLLDLSTRRLIRVIYNFCNSVRVMMTAQLGSLKNVMLVLGYNRKNTEGTQKQKEIQSCLTVWDINLPHEVQNLEKHIEVRKELAEKMRRTSVE DIP protein Q5S007 original sequence version: 36 DIP protein Q5S007 original sequence version: 68 DIP protein Q5S007 original sequence version: 64 26142A0CECBBC3F4 lrrk2_mouse Leucine-rich repeat serine/threonine-protein kinase 2 Lrrk2 protein protein mouse Mus musculus Mouse MASGACQGCEEEEEEEALKKLIVRLNNVQEGKQIETLLQLLEDMLVFTYSDRASKLFEDKNFHVPLLIVLDSYMRVASVQQAGWSLLCKLIEVCPGTLQSLIGPQDIGNDWEVLGIHRLILKMLTVHHANVNLSIVGLKALDLLLDSGKLTLLILDEECDIFLLIFDAMHRYSANDEVQKLGCKALHVLFERVSEEQLTEFVENKDYTILLSTFGSFRRDKEIVYHVLCCLHSLAVTCSNVEVLMSGNVRCYNLVVEAMKAFPTNENIQEVSCSLFQKLTLGNFFNILVLNEVHVFVVKAVRQYPENAALQISALSCLALLTETIFLNQDLEERSETQEQSEEEDSEKLFWLEPCYKALVRHRKDKHVQEAACWALNNLLMYQNSLHEKIGDEDGQFPAHREVMLSMLMHSSSKDVFQAAAHALSTLLEQNVNFRKILLAKGVYLNVLELMQKHAHAPEVAESGCKMLSHLFEGSNPSLDTMAAVVPKILTVMKAHGTSLSVQLEALRAILHFVVPGLLEESREDSQCRPNVLRKQCFRTDIHKLVLVALNRFIGNPGIQKCGLKVISSLAHLPDATETLSLQGAVDSVLHTLQMYPDDQEIQCLGLHLMGCLMTKKNFCIGTGHLLAKILASTLQRFKDVAEVQTTGLQTTLSILELSVSFSKLLVHYSFDVVIFHQMSSSVVEQKDEQFLNLCCKCFAKVAVDDELKNTMLERACDQNNSIMVECLLLLGADANQVKGATSLIYQVCEKESSPKLVELLLNGGCREQDVRKALTVSIQKGDSQVISLLLRKLALDLANNSICLGGFGIGKIDPSWLGPLFPDKSSNLRKQTNTGSVLARKVLRYQMRNTLQEGVASGSDGKFSEDALAKFGEWTFIPDSSMDSVFGQSDDLDSEGSESSFLVKRKSNSISVGEVYRDLALQRCSPNAQRHSNSLGPVFDHEDLLRRKRKILSSDESLRSSRLPSHMRQSDSSSSLASEREHITSLDLSANELKDIDALSQKCCLSSHLEHLTKLELHQNSLTSFPQQLCETLKCLIHLDLHSNKFTSFPSFVLKMPRITNLDASRNDIGPTVVLDPAMKCPSLKQLNLSYNQLSSIPENLAQVVEKLEQLLLEGNKISGICSPLSLKELKILNLSKNHIPSLPGDFLEACSKVESFSARMNFLAAMPALPSSITSLKLSQNSFTCIPEAIFSLPHLRSLDMSHNNIECLPGPAHWKSLNLRELIFSKNQISTLDFSENPHVWSRVEKLHLSHNKLKEIPPEIGCLENLTSLDVSYNLELRSFPNEMGKLSKIWDLPLDGLHLNFDFKHVGCKAKDIIRFLQQRLKKAVPYNRMKLMIVGNTGSGKTTLLQQLMKMKKPELGMQGATVGIDVRDWSIQIRGKRRKDLVLNVWDFAGREEFYSTHPHFMTQRALYLAVYDLSKGQAEVDAMKPWLFNIKARASSSPVILVGTHLDVSDEKQRKACISKITKELLNKRGFPTIRDYHFVNATEESDALAKLRKTIINESLNFKIRDQPVVGQLIPDCYVELEKIILSERKAVPTEFPVINRKHLLQLVNEHQLQLDENELPHAVHFLNESGVLLHFQDPALQLSDLYFVEPKWLCKVMAQILTVKVDGCLKHPKGIISRRDVEKFLSKKKRFPKNYMMQYFKLLEKFQIALPIGEEYLLVPSSLSDHRPVIELPHCENSEIIIRLYEMPYFPMGFWSRLINRLLEISPFMLSGRERALRPNRMYWRQGIYLNWSPEAYCLVGSEVLDNRPESFLKITVPSCRKGCILLGRVVDHIDSLMEEWFPGLLEIDICGEGETLLKKWALYSFNDGEEHQKILLDELMKKAEEGDLLINPDQPRLTIPISQIAPDLILADLPRNIMLNNDELEFEEAPEFLLGDGSFGSVYRAAYEGEEVAVKIFNKHTSLRLLRQELVVLCHLHHPSLISLLAAGIRPRMLVMELASKGSLDRLLQQDKASLTRTLQHRIALHVADGLRYLHSAMIIYRDLKPHNVLLFTLYPNAAIIAKIADYGIAQYCCRMGIKTSEGTPGFRAPEVARGNVIYNQQADVYSFGLLLHDIWTTGSRIMEGLRFPNEFDELAIQGKLPDPVKEYGCAPWPMVEKLITKCLKENPQERPTSAQVFDILNSAELICLMRHILIPKNIIVECMVATNLNSKSATLWLGCGNTEKGQLSLFDLNTERYSYEEVADSRILCLALVHLAAEKESWVVCGTQSGALLVINVEEETKRHTLEKMTDSVTCLHCNSLAKQSKQSNFLLVGTADGNLMIFEDKAVKCKGAAPLKTLHIGDVSTPLMCLSESLNSSERHITWGGCGTKVFSFSNDFTIQKLIETKTNQLFSYAAFSDSNIIALAVDTALYIAKKNSPVVEVWDKKTEKLCELIDCVHFLKEVMVKLNKESKHQLSYSGRVKALCLQKNTALWIGTGGGHILLLDLSTRRVIRTIHNFCDSVRAMATAQLGSLKNVMLVLGYKRKSTEGIQEQKEIQSCLSIWDLNLPHEVQNLEKHIEVRTELADKMRKTSVE DIP protein Q5S006 original sequence version: 57 706E25C173E36F98 syua_human Alpha-synuclein Non-A beta component of AD amyloid Non-A4 component of amyloid precursor SNCA NACP PARK1 protein protein human Homo sapiens Human MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVATVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGSIAAATGFVKKDQLGKNEEGAPQEGILEDMPVDPDNEAYEMPSEEGYQDYEPEA DIP protein P37840 original sequence version: 124 DIP protein P37840 original sequence version: 175 DIP protein P37840 original sequence version: 178 6BB2F12128931663 snca-lrrk2 1 7 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association 1A lrrk2-snca 3 10 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 1B snca-lrrk2-1 4 10 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q5S007:p.Gly2019Ser mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain G S Wed May 02 15:24:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly2019ser' Tue Jun 30 17:27:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly2019Ser' physical association physical association 1C lrrk2-snca-2 6 10 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component colocalization colocalization 2A-C lrrk2-snca-3 6 8 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component possible_phosph-ser129 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain colocalization colocalization 2D,F-H I am dubious about adding this as a separate interaction- as I dont think the use of a phospho-specific antibody would guarantee that SYN had not been phosphorylated after, rather than before, the interaction had occurred.... lrrk2-snca-4 5 8 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component colocalization colocalization 2E lrrk2-snca-5 2 10 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component colocalization colocalization S4E-G