matrixdb Institut de Biologie et de Chimie des Prot\u00c3\u00a9ines, 7, passage du Vercors 69 367 Lyon cedex 07 FRANCE matrixdb@ibcp.fr http://matrixdb.ibcp.fr Transglutaminase-2 interaction with heparin: identification of a heparin binding site that regulates cell adhesion to fibronectin-transglutaminase-2 matrix. Transglutaminase-2 interaction with heparin: identification of a heparin binding site that regulates cell adhesion to fibronectin-transglutaminase-2 matrix. J. Biol. Chem. (0021-9258) 2012 imex curation Lortat-Jacob H., Burhan I., Scarpellini A., Thomas A., Imberty A., Vivès R.R., Johnson T., Gutierrez A., Verderio E.A. elisabetta.verderio-edwards@ntu.ac.uk 16-Apr-2013: Contacted by IntAct-Help. in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Lortat-Jacob H., Burhan I., Scarpellini A., Thomas A., Imberty A., Vivès R.R., Johnson T., Gutierrez A., Verderio E.A. elisabetta.verderio-edwards@ntu.ac.uk Accepted 2013-APR-05 by ORCHARD 16-Apr-2013: Contacted by IntAct-Help. J. Biol. Chem. (0021-9258) 2012 imex curation Transglutaminase-2 interaction with heparin: identification of a heparin binding site that regulates cell adhesion to fibronectin-transglutaminase-2 matrix. Transglutaminase-2 interaction with heparin: identification of a heparin binding site that regulates cell adhesion to fibronectin-transglutaminase-2 matrix. J. Biol. Chem. (0021-9258) 2012 imex curation Lortat-Jacob H., Burhan I., Scarpellini A., Thomas A., Imberty A., Vivès R.R., Johnson T., Gutierrez A., Verderio E.A. elisabetta.verderio-edwards@ntu.ac.uk 16-Apr-2013: Contacted by IntAct-Help. in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa antibody detection identification by antibody antibody detection Lortat-Jacob H., Burhan I., Scarpellini A., Thomas A., Imberty A., Vivès R.R., Johnson T., Gutierrez A., Verderio E.A. elisabetta.verderio-edwards@ntu.ac.uk Accepted 2013-APR-05 by ORCHARD 16-Apr-2013: Contacted by IntAct-Help. J. Biol. Chem. (0021-9258) 2012 imex curation Transglutaminase-2 interaction with heparin: identification of a heparin binding site that regulates cell adhesion to fibronectin-transglutaminase-2 matrix. Transglutaminase-2 interaction with heparin: identification of a heparin binding site that regulates cell adhesion to fibronectin-transglutaminase-2 matrix. J. Biol. Chem. (0021-9258) 2012 imex curation Lortat-Jacob H., Burhan I., Scarpellini A., Thomas A., Imberty A., Vivès R.R., Johnson T., Gutierrez A., Verderio E.A. elisabetta.verderio-edwards@ntu.ac.uk 16-Apr-2013: Contacted by IntAct-Help. in vitro In vitro enzymatic study enzymatic study predetermined predetermined participant predetermined Lortat-Jacob H., Burhan I., Scarpellini A., Thomas A., Imberty A., Vivès R.R., Johnson T., Gutierrez A., Verderio E.A. elisabetta.verderio-edwards@ntu.ac.uk Accepted 2013-APR-05 by ORCHARD 16-Apr-2013: Contacted by IntAct-Help. J. Biol. Chem. (0021-9258) 2012 imex curation finc_human Fibronectin FN1 FN Cold-insoluble globulin protein protein human Homo sapiens Human MLRGPGPGLLLLAVQCLGTAVPSTGASKSKRQAQQMVQPQSPVAVSQSKPGCYDNGKHYQINQQWERTYLGNALVCTCYGGSRGFNCESKPEAEETCFDKYTGNTYRVGDTYERPKDSMIWDCTCIGAGRGRISCTIANRCHEGGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEWTCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMMVDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIGDTWSKKDNRGNLLQCICTGNGRGEWKCERHTSVQTTSSGSGPFTDVRAAVYQPQPHPQPPPYGHCVTDSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTEGRQDGHLWCSTTSNYEQDQKYSFCTDHTVLVQTRGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNMKWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVMYRIGDQWDKQHDMGHMMRCTCVGNGRGEWTCIAYSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNCTCFGQGRGRWKCDPVDQCQDSETGTFYQIGDSWEKYVHGVRYQCYCYGRGIGEWHCQPLQTYPSSSGPVEVFITETPSQPNSHPIQWNAPQPSHISKYILRWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVYEGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNTVTGETTPFSPLVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEDGEQSLILSTSQTTAPDAPPDTTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELNLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVVIQQETTGTPRSDTVPSPRDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKVFAVSHGRESKPLTAQQTTKLDAPTNLQFVNETDSTVLVRWTPPRAQITGYRLTVGLTRRGQPRQYNVGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPKATGVFTTLQPGSSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEYNVSVYTVKDDKESVPISDTIIPEVPQLTDLSFVDITDSSIGLRWTPLNSSTIIGYRITVVAAGEGIPIFEDFVDSSVGYYTVTGLEPGIDYDISVITLINGGESAPTTLTQQTAVPPPTDLRFTNIGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYVVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHFSGRPREDRVPHSRNSITLTNLTPGTEYVVSIVALNGREESPLLIGQQSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISINYRTEIDKPSQMQVTDVQDNSISVKWLPSSSPVTGYRVTTTPKNGPGPTKTKTAGPDQTEMTIEGLQPTVEYVVSVYAQNPSGESQPLVQTAVTNIDRPKGLAFTDVDVDSIKIAWESPQGQVSRYRVTYSSPEDGIHELFPAPDGEEDTAELQGLRPGSEYTVSVVALHDDMESQPLIGTQSTAIPAPTDLKFTQVTPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKEINLAPDSSSVVVSGLMVATKYEVSVYALKDTLTSRPAQGVVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQTPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVVIDASTAIDAPSNLRFLATTPNSLLVSWQPPRARITGYIIKYEKPGSPPREVVPRPRPGVTEATITGLEPGTEYTIYVIALKNNQKSEPLIGRKKTDELPQLVTLPHPNLHGPEILDVPSTVQKTPFVTHPGYDTGNGIQLPGTSGQQPSVGQQMIFEEHGFRRTTPPTTATPIRHRPRPYPPNVGEEIQIGHIPREDVDYHLYPHGPGLNPNASTGQEALSQTTISWAPFQDTSEYIISCHPVGTDEEPLQFRVPGTSTSATLTGLTRGATYNVIVEALKDQQRHKVREEVVTVGNSVNEGLNQPTDDSCFDPYTVSHYAVGDEWERMSESGFKLLCQCLGFGSGHFRCDSSRWCHDNGVNYKIGEKWDRQGENGQMMSCTCLGNGKGEFKCDPHEATCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNCRRPGGEPSPEGTTGQSYNQYSQRYHQRTNTNVNCPIECFMPLDVQADREDSRE DIP protein P02751 original sequence version: 119 DIP protein P02751 original sequence version: 131 DIP protein P02751 original sequence version: 154 DIP protein P02751 original sequence version: 156 DIP protein P02751 original sequence version: 157 DIP protein P02751 original sequence version: 155 DIP protein P02751 original sequence version: 194 6C436A7A5FEE6DEB r283 R283 transglutaminase inhibitor small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) tgm2_human Protein-glutamine gamma-glutamyltransferase 2 TGM2 Tissue transglutaminase Transglutaminase C Transglutaminase-2 Transglutaminase H Erythrocyte transglutaminase Heart G alpha(h) Isopeptidase TGM2 Protein G alpha(h) Protein-glutamine deamidase TGM2 Protein-glutamine dopaminyltransferase TGM2 Protein-glutamine histaminyltransferase TGM2 Protein-glutamine noradrenalinyltransferase TGM2 Protein-glutamine serotonyltransferase TGM2 Transglutaminase II protein protein human Homo sapiens Human MAEELVLERCDLELETNGRDHHTADLCREKLVVRRGQPFWLTLHFEGRNYEASVDSLTFSVVTGPAPSQEAGTKARFPLRDAVEEGDWTATVVDQQDCTLSLQLTTPANAPIGLYRLSLEASTGYQGSSFVLGHFILLFNAWCPADAVYLDSEEERQEYVLTQQGFIYQGSAKFIKNIPWNFGQFEDGILDICLILLDVNPKFLKNAGRDCSRRSSPVYVGRVVSGMVNCNDDQGVLLGRWDNNYGDGVSPMSWIGSVDILRRWKNHGCQRVKYGQCWVFAAVACTVLRCLGIPTRVVTNYNSAHDQNSNLLIEYFRNEFGEIQGDKSEMIWNFHCWVESWMTRPDLQPGYEGWQALDPTPQEKSEGTYCCGPVPVRAIKEGDLSTKYDAPFVFAEVNADVVDWIQQDDGSVHKSINRSLIVGLKISTKSVGRDEREDITHTYKYPEGSSEEREAFTRANHLNKLAEKEETGMAMRIRVGQSMNMGSDFDVFAHITNNTAEEYVCRLLLCARTVSYNGILGPECGTKYLLNLNLEPFSEKSVPLCILYEKYRDCLTESNLIKVRALLVEPVINSYLLAERDLYLENPEIKIRILGEPKQKRKLVAEVSLQNPLPVALEGCTFTVEGAGLTEEQKTVEIPDPVEAGEEVKVRMDLLPLHMGLHKLVVNFESDKLKAVKGFRNVIIGPA DIP protein P21980 original sequence version: 125 7DA33FF335DE7B37 heparin Heparin small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) boc-don Boc-DON-Gln-Ile-Val-OMe small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) tgm2-heparin 1 7 unspecified role unspecified role bait bait pig-gastric mucosa Sus scrofa gastric mucosa gastric_mucosa gastric mucosa Transglutaminase-2 6 unspecified role unspecified role prey prey purified purified P21980:p.[Lys202Ser;Lys205Ser] mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain K S certain certain sequence position certain certain certain sequence position certain K S Wed May 02 15:05:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys202ser,lys205ser' Tue Jun 30 20:10:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys202Ser;Lys205Ser]' P21980:p.Arg262Ser mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain R S Wed May 02 15:55:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg262ser' Tue Jun 30 16:49:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg262Ser' P21980:p.Lys634Ser mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain K S Wed May 02 14:56:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys634ser' Tue Jun 30 19:08:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys634Ser' P21980:p.Arg263Ser mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain R S Wed May 02 16:00:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg263ser' Tue Jun 30 15:45:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg263Ser' P21980:p.Arg28Ser mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain R S Wed May 02 15:31:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg28ser' Tue Jun 30 20:08:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg28Ser' 6his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag predetermined featur predetermined feature predetermined featur c-term range C-terminal range c-term range c-term range C-terminal range c-term range P21980:p.Arg19Ser mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain R S Wed May 02 15:45:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg19ser' Tue Jun 30 18:49:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg19Ser' ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 3 fn1-tgm2-2 3 4 enzyme target enzyme target substrate substrate neutral component neutral component human-plasma homo sapiens blood plasma plasma blood plasma 6 enzyme enzyme neutral component neutral component P21980:p.Arg263Ser mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain R S Wed May 02 17:36:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg263ser' Tue Jun 30 17:28:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg263Ser' 6his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag predetermined featur predetermined feature predetermined featur c-term range C-terminal range c-term range c-term range C-terminal range c-term range P21980:p.Arg262Ser mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain R S Wed May 02 18:30:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg262ser' Tue Jun 30 19:36:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg262Ser' P21980:p.Lys265Ser mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain K S Wed May 02 19:25:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys265ser' Tue Jun 30 16:49:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys265Ser' P21980:p.Lys634Ser mutation increasing mutation increasing interaction mutation increasing predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain K S Wed May 02 17:38:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys634ser' Tue Jun 30 16:48:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys634Ser' P21980:p.Arg28Ser mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain R S Wed May 02 15:41:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg28ser' Tue Jun 30 17:02:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg28Ser' P21980:p.Arg580Ser mutation increasing mutation increasing interaction mutation increasing predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain R S Wed May 02 17:21:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg580ser' Tue Jun 30 17:53:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg580Ser' ecoli Escherichia coli (strain K12) enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction Table 1 heparin-tgm2 1 8 inhibitor inhibitor neutral component neutral component 7 unspecified role unspecified role bait bait biotin tag biotin tag biotin tag predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined pig-gastric mucosa Sus scrofa gastric mucosa gastric_mucosa gastric mucosa 6 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 4 tgm2-fn1 2 6 unspecified role unspecified role bait bait 6his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag predetermined featur predetermined feature predetermined featur c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role unspecified role unspecified role human-plasma homo sapiens blood plasma plasma blood plasma direct interaction direct interaction Suppl Fig 3A heparin-fn1-1 2 7 unspecified role unspecified role bait bait pig-gastric mucosa Sus scrofa gastric mucosa gastric_mucosa gastric mucosa 4 unspecified role unspecified role prey prey human-plasma homo sapiens blood plasma plasma blood plasma 6 unspecified role unspecified role prey prey 6his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag predetermined featur predetermined feature predetermined featur c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Suppl Fig 3C heparin-tgm2-2 2 6 unspecified role unspecified role unspecified role unspecified role pig-gastric mucosa Sus scrofa gastric mucosa gastric_mucosa gastric mucosa 7 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) direct interaction direct interaction Suppl Fig 3C heparin-tgm2-3 1 7 unspecified role unspecified role bait bait biotin tag biotin tag biotin tag predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined pig-gastric mucosa Sus scrofa gastric mucosa gastric_mucosa gastric mucosa 6 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) 5 inhibitor inhibitor neutral component neutral component direct interaction direct interaction Figure 4