UniProt UniProt http://www.uniprot.org RpgrORF15 connects to the usher protein network through direct interactions with multiple whirlin isoforms. RpgrORF15 connects to the usher protein network through direct interactions with multiple whirlin isoforms. Invest. Ophthalmol. Vis. Sci. (0146-0404) 2012 imex curation Wright R.N., Hong D.H., Perkins B. bperkins@mail.bio.tamu.edu 10-Mar-2014: Contacted by IntAct-Help. C57BL/6 mouse retinas cDNA Only protein-protein interactions mouse-retina Mus musculus retina retina retinal tissue anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wright R.N., Hong D.H., Perkins B. bperkins@mail.bio.tamu.edu Accepted 2014-FEB-19 by ORCHARD C57BL/6 mouse retinas cDNA Invest. Ophthalmol. Vis. Sci. (0146-0404) 2012 Only protein-protein interactions imex curation RpgrORF15 connects to the usher protein network through direct interactions with multiple whirlin isoforms. RpgrORF15 connects to the usher protein network through direct interactions with multiple whirlin isoforms. Invest. Ophthalmol. Vis. Sci. (0146-0404) 2012 imex curation Wright R.N., Hong D.H., Perkins B. bperkins@mail.bio.tamu.edu 10-Mar-2014: Contacted by IntAct-Help. C57BL/6 mouse retinas cDNA Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Wright R.N., Hong D.H., Perkins B. bperkins@mail.bio.tamu.edu Accepted 2014-FEB-19 by ORCHARD C57BL/6 mouse retinas cDNA Invest. Ophthalmol. Vis. Sci. (0146-0404) 2012 Only protein-protein interactions imex curation RpgrORF15 connects to the usher protein network through direct interactions with multiple whirlin isoforms. RpgrORF15 connects to the usher protein network through direct interactions with multiple whirlin isoforms. Invest. Ophthalmol. Vis. Sci. (0146-0404) 2012 imex curation Wright R.N., Hong D.H., Perkins B. bperkins@mail.bio.tamu.edu 10-Mar-2014: Contacted by IntAct-Help. C57BL/6 mouse retinas cDNA Only protein-protein interactions mouse-retina Mus musculus retina retina retinal tissue fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Wright R.N., Hong D.H., Perkins B. Interaction observed in both intact retinas and isolated photoreceptors. bperkins@mail.bio.tamu.edu Accepted 2014-FEB-19 by ORCHARD C57BL/6 mouse retinas cDNA Invest. Ophthalmol. Vis. Sci. (0146-0404) 2012 Only protein-protein interactions imex curation RpgrORF15 connects to the usher protein network through direct interactions with multiple whirlin isoforms. RpgrORF15 connects to the usher protein network through direct interactions with multiple whirlin isoforms. Invest. Ophthalmol. Vis. Sci. (0146-0404) 2012 imex curation Wright R.N., Hong D.H., Perkins B. bperkins@mail.bio.tamu.edu 10-Mar-2014: Contacted by IntAct-Help. C57BL/6 mouse retinas cDNA Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Wright R.N., Hong D.H., Perkins B. bperkins@mail.bio.tamu.edu Accepted 2014-FEB-19 by ORCHARD C57BL/6 mouse retinas cDNA Invest. Ophthalmol. Vis. Sci. (0146-0404) 2012 Only protein-protein interactions imex curation whrn_mouse Whirlin Whrn Dfnb31 Kiaa1526 protein protein mouse Mus musculus Mouse MNAQLDGLSVSSSSTGSLGSAAAAAGGGGGAGLRLLSANVRQLHQALTALLSEPEREQFTHCLNAYHARRNVFDLVRTLRVLLDSPVKRRLLPMLRLVIPRSDQLLFDQYTAEGLYLPATTPYRQPAWAAPDGAGPGEVRLVSLRRAKAHEGLGFSIRGGSEHGVGIYVSLVEPGSLAEKEGLRVGDQILRVNDKSLARVTHAEAVKALKGSKKLVLSVYSAGRIPGGYVTNHIYTWVDPQGRSTSPPSSLPQPHGSTLRQREDDRRSTLHLLQSGDEKKVNLVLGDGRSLGLTIRGGAEYGLGIYITGVDPGSEAESSGLKVGDQILEVNGRSFLNILHDEAVKLLKSSRHLILTVKDVGRLPHARTTVDQTKWIASSRIGESVANSAGFPGDHTEEGTSKPGFYKGPAGSQVTLSSLGNQTRALLDDQARHLLTEQERATMMYYLAQYRGGTISVEAMVMALFELLNTHAKFSLLSEVRSIISPQDLDRFDHLVLRREIESMKARQPPGPGVGDTYSMVSYSDTGSSTGSHGTSTTVSSARERLLWLIDLMENTLDLEGTGETTQGSTNALPDVSVDDVKSPSEDLPGIKPPPPPPPLAQGHDRLLGQPRKPGREDPAPLSSAAHSGIVFSAPRNRSPPPGTAPTPGPSSAQDSPSSPIYASISHANPSSRKPLDTHLALVNQHPIGPFPRVQSPPHLKSPPAETPGAGACLPPPSPSEHPDAVGANQHFVLVEVHRPDSEPDVNEVRALPQTRTASTLSQLSDSGQTLSEDSGVDAGETEASTSGRGRQTASAKNKNGKEQPRTERTAEGANKPPGLLEPTSTLVRVRKSAATLGIAIEGGANTRQPLPRIVTIQRGGSAHNCGQLKVGHVILEVNGQTLRGKEHKEAARIIAEAFKTKERDYIDFLVTEFNVML DIP protein Q80VW5 original sequence version: 94 Necessary for elongation and maintenance of inner and outer hair cell stereocilia in the organ of Corti in the inner ear mint Defects in Whrn are the cause of the phenotype whirler (wi). Mutants are characterized by deafness due to malformation of the cochlear inner and outer hair cells and by circling behavior. Stereocilia are shorter and wider than in wild-type animals and there is a decrease in the number of actin filaments in inner and outer hair cells. The number of outer hair cell stereocilia is reduced with increased spacing between them 7D5EA44DE0645AA4 q80vw5-12 Whirlin Whrn Dfnb31 Kiaa1526 WhirlinNT1 protein protein mouse Mus musculus Mouse MNAQLDGLSVSSSSTGSLGSAAAAAGGGGGAGLRLLSANVRQLHQALTALLSEPEREQFTHCLNAYHARRNVFDLVRTLRVLLDSPVKRRLLPMLRLVIPRSDQLLFDQYTAEGLYLPATTPYRQPAWAAPDGAGPGEVRLVSLRRAKAHEGLGFSIRGGSEHGVGIYVSLVEPGSLAEKEGLRVGDQILRVNDKSLARVTHAEAVKALKGSKKLVLSVYSAGRIPGGYVTNHIYTWVDPQGRSTSPPSSLPQPHGSTLRQREDDRRSTLHLLQSGDEKKVNLVLGDGRSLGLTIRGGAEYGLGIYITGVDPGSEAESSGLK May be due to intron retention. q9r0x5-5 X-linked retinitis pigmentosa GTPase regulator ORF15 Rpgr protein protein mouse Mus musculus Mouse MAESESLVPDTGAVFTFGKTKFAENIPSKFWFKNDIPICLSCGDEHTAIVTGNNKLYMFGSNNWGQLGLGSKAAIIKPTCIKALKPEKVKLAACGRNHTLVSTDTGGVYAAGGNNEGQLGLGDTDDRDTFHQIVFFTPADTIKQLSAGANTSAALTEDGKLFMWGDNSEGQIGLEDKSNVCIPHEVTVGKPISWISCGYYHSAFVTMDGELYTFGEPENGKLGLPNELLMNHRSPQRVLGIPERVIQVACGGGHTVVLTEKVVYAFGLGQFGQLGLGTFLFETSEPKIIERIKDQKICHISCGENHTALMTELGLLYTFGDGRHGKLGLGMENFTNQFFPTLCSNFLRFAVQLIACGGCHMLVFATPRLGTIDEPKFEDVYEPYISTGSFSINDLSPRSSLNRSLSARLRRRERERPPCSASMVGTLPPLEGTSASTSAYFYPSSPPFHLSVNNYPEKSPSESMEPLDSDYFEDKMNKDTETENSSAVDSENFGETNDILNMTHMMTTSSNEKLLDFSPIQKQQNQDTFEKVMESTPCTENEDSYEYEEMSKIKEVTVYKQYLAKGIYMIRPAEILEAFSDEEVGNGLDQVEEPRVFTDGKGLQSKQVGKESDEEIVSEKKTEVMEVADVKKIRESEENSKSDSLFDDLPDKTMNSESEDNKDIAEERRSSEQNMTFDSETELVEEPDSYMECERHSEQDSAEELEQPKLVEYSSEEKDEKDEKDDDEVETENLWYDRNCTEQETENVFRATRFFPKFDLKHDHLSGIPEEQEGPEDSEGNVVVEQVVQAQKENLEFEGDRKEAKAEAPSDVITEKEVSESERESGGEREDRSEGDGDQICEKVSLETEHLQRAQGKQERKKGKDKRARCILDMKEREEDKGWEKGSEGGDKMKRDEGNQEKRKKEMEERDAGDERSEEEEGEEEEPEEGEKEEGGEEEEGTSEDQSREDEGDRQEKEGRREGKGRQEDGREGWKEGEEQEQEEEIEEGEEEEREGEEEGGEEEGEGEGEREEEGEGEEEGEGEEEGEGEEEGEGEEEGEGEEEGEGEEEGEGEEEGEGEEDGEGEEDGEGEEEGEGEEEGEREEDGEGEEDGEGEEEGEGEEEGEGEEEGEGEEEGEGEGEEEGEGEWEGEEEGEGEEEGEGEEEGEGEEEGEGEEEGEGEEEGGEDDEGEELEKKKGDITEEEEEEEEGQEGDEREREEHGSCEDDVEEDKTYDREEGEYKKAIGKVADNESQEDRKQSPKVSKINGSMKYGRHGTYSEKPITNLGKTQPSKMPMESRQLVENGLLGSERFWSDVLPLYLELK No experimental confirmation available. C2630A87241006B5 rpgr_mouse X-linked retinitis pigmentosa GTPase regulator Rpgr protein protein mouse Mus musculus Mouse MAESESLVPDTGAVFTFGKTKFAENIPSKFWFKNDIPICLSCGDEHTAIVTGNNKLYMFGSNNWGQLGLGSKAAIIKPTCIKALKPEKVKLAACGRNHTLVSTDTGGVYAAGGNNEGQLGLGDTDDRDTFHQIVFFTPADTIKQLSAGANTSAALTEDGKLFMWGDNSEGQIGLEDKSNVCIPHEVTVGKPISWISCGYYHSAFVTMDGELYTFGEPENGKLGLPNELLMNHRSPQRVLGIPERVIQVACGGGHTVVLTEKVVYAFGLGQFGQLGLGTFLFETSEPKIIERIKDQKICHISCGENHTALMTELGLLYTFGDGRHGKLGLGMENFTNQFFPTLCSNFLRFAVQLIACGGCHMLVFATPRLGTIDEPKFEDVYEPYISTGSFSINDLSPRSSLNRSLSARLRRRERERPPCSASMVGTLPPLEGTSASTSAYFYPSSPPFHLSVNNYPEKSPSESMEPLDSDYFEDKMNKDTETENSSAVDSENFGETNDILNMTHMMTTSSNEKLLDFSPIQKQQNQDTFEKVMESTPCTENEDSYEYEEMSKIKEVTVYKQYLAKGIYMIRPAEILEAFSDEEVGNGLDQVEEPRVFTDGKGLQSKQVGKESDEEIVSEKKTEVMEVADVKKIRESEENSKSDSLFDDLPDKTMNSESEDNKDIAEERRSSEQNMTFDSETELVEEPDSYMECERHSEQDSAEELEQPKLVEYSSEEKDEKDEKDDDEVETENLWYDRNCTEQETENVFRATRFFPKFDLKHDHLSGIPEEQEGPEDSEGNVVVEQVVQAQKENLEFEGDRKEAKAEAPSDVITEKEAPQLSETVKPEEGEMDEEISILNVEDTVEEERKEGEKEIVEEGSIPETEGSETIDITDEKLDEVLKEEDSASLLQRALREYNENPKGHMYDRVKSSSSEILGGNDPTSKDIKKAKKISFFNRMSLTGQKLMQNTNDPLPEIKPIGDQIALQSDKKDANQNHMGQNLQDSTTPNMEGKSKSCTIL DIP protein Q9R0X5 original sequence version: 68 021997395B74E6CA rpgr-q80vw5_12 4 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range range range physical association physical association The UniProt entry for Q9R0X5 isoform 5 (ORF15) differs in length from that given in the paper. dfnb31-rpgr 2 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind range range range range region sufficient to bind sufficient binding region sufficient to bind range range range range human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 6 unspecified role unspecified role bait bait n-terminal mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range pdz1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain pdz2 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range ecoli Escherichia coli (strain K12) 15 20 16 19 physical association physical association Fig 3B The UniProt entries for Q9R0X5 isoform 5 (ORF15) and Q80VW5 isoform 12 differ in length from those given in the paper. rpgr-dfnb31 1 5 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association Fig. 3D dfnb31-rpgr-1 3 5 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 4