mbinfo MBInfo http://www.mechanobio.info/ FAK promotes recruitment of talin to nascent adhesions to control cell motility. FAK promotes recruitment of talin to nascent adhesions to control cell motility. J. Cell Biol. (0021-9525) 2012 imex curation Lawson C., Lim S.T., Uryu S., Chen X.L., Calderwood D.A., Schlaepfer D.D. Participant detection: The colocalization detected by fluorescence patterns were then quantitated on a pixel-by-pixel basis and the Pearson's correlation coefficient was calculated. Some of the coimmunoprecipitations (Figs 1C, 2B, 3A, 5D) and colocalization (Figs 2C, 2D) experiments could not be curated since the specific talin isoform involved in these interactions was not specified and could not be identified. Coimmunoprecipitations (Fig 4A) and colocalizations (Fig 4B, 4C, S2A, S3A, S3B, S3C, S3D) involving GFP-FAK could not be curated since the source of GFP-FAK could not be identified. Direct binding assay (Fig 3E, S2C, S2D) could not be curated since the source of GST-talin could not be identified. dschlaepfer@ucsd.edu 8-May-2012: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. pull down pull down anti tag western anti tag western blot anti tag western Lawson C., Lim S.T., Uryu S., Chen X.L., Calderwood D.A., Schlaepfer D.D. Participant detection: The colocalization detected by fluorescence patterns were then quantitated on a pixel-by-pixel basis and the Pearson's correlation coefficient was calculated. Some of the coimmunoprecipitations (Figs 1C, 2B, 3A, 5D) and colocalization (Figs 2C, 2D) experiments could not be curated since the specific talin isoform involved in these interactions was not specified and could not be identified. Coimmunoprecipitations (Fig 4A) and colocalizations (Fig 4B, 4C, S2A, S3A, S3B, S3C, S3D) involving GFP-FAK could not be curated since the source of GFP-FAK could not be identified. Direct binding assay (Fig 3E, S2C, S2D) could not be curated since the source of GST-talin could not be identified. dschlaepfer@ucsd.edu Accepted 2013-APR-26 by ORCHARD 8-May-2012: Contacted by IntAct-Help. J. Cell Biol. (0021-9525) 2012 Only protein-protein interactions imex curation FAK promotes recruitment of talin to nascent adhesions to control cell motility. FAK promotes recruitment of talin to nascent adhesions to control cell motility. J. Cell Biol. (0021-9525) 2012 imex curation Lawson C., Lim S.T., Uryu S., Chen X.L., Calderwood D.A., Schlaepfer D.D. Participant detection: The colocalization detected by fluorescence patterns were then quantitated on a pixel-by-pixel basis and the Pearson's correlation coefficient was calculated. Some of the coimmunoprecipitations (Figs 1C, 2B, 3A, 5D) and colocalization (Figs 2C, 2D) experiments could not be curated since the specific talin isoform involved in these interactions was not specified and could not be identified. Coimmunoprecipitations (Fig 4A) and colocalizations (Fig 4B, 4C, S2A, S3A, S3B, S3C, S3D) involving GFP-FAK could not be curated since the source of GFP-FAK could not be identified. Direct binding assay (Fig 3E, S2C, S2D) could not be curated since the source of GST-talin could not be identified. dschlaepfer@ucsd.edu 8-May-2012: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. pull down pull down western blot western blot Immuno blot Immuno blot Lawson C., Lim S.T., Uryu S., Chen X.L., Calderwood D.A., Schlaepfer D.D. Participant detection: The colocalization detected by fluorescence patterns were then quantitated on a pixel-by-pixel basis and the Pearson's correlation coefficient was calculated. Some of the coimmunoprecipitations (Figs 1C, 2B, 3A, 5D) and colocalization (Figs 2C, 2D) experiments could not be curated since the specific talin isoform involved in these interactions was not specified and could not be identified. Coimmunoprecipitations (Fig 4A) and colocalizations (Fig 4B, 4C, S2A, S3A, S3B, S3C, S3D) involving GFP-FAK could not be curated since the source of GFP-FAK could not be identified. Direct binding assay (Fig 3E, S2C, S2D) could not be curated since the source of GST-talin could not be identified. dschlaepfer@ucsd.edu Accepted 2013-APR-26 by ORCHARD 8-May-2012: Contacted by IntAct-Help. J. Cell Biol. (0021-9525) 2012 Only protein-protein interactions imex curation FAK promotes recruitment of talin to nascent adhesions to control cell motility. FAK promotes recruitment of talin to nascent adhesions to control cell motility. J. Cell Biol. (0021-9525) 2012 imex curation Lawson C., Lim S.T., Uryu S., Chen X.L., Calderwood D.A., Schlaepfer D.D. Participant detection: The colocalization detected by fluorescence patterns were then quantitated on a pixel-by-pixel basis and the Pearson's correlation coefficient was calculated. Some of the coimmunoprecipitations (Figs 1C, 2B, 3A, 5D) and colocalization (Figs 2C, 2D) experiments could not be curated since the specific talin isoform involved in these interactions was not specified and could not be identified. Coimmunoprecipitations (Fig 4A) and colocalizations (Fig 4B, 4C, S2A, S3A, S3B, S3C, S3D) involving GFP-FAK could not be curated since the source of GFP-FAK could not be identified. Direct binding assay (Fig 3E, S2C, S2D) could not be curated since the source of GST-talin could not be identified. dschlaepfer@ucsd.edu 8-May-2012: Contacted by IntAct-Help. Only protein-protein interactions human-uvec Human Umbilical Vein Endothelial Cells (HUVEC) huvec umbilical_vein_endothelium Umbilical vein endothelial cell [Umbilical vein endothelium] fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Lawson C., Lim S.T., Uryu S., Chen X.L., Calderwood D.A., Schlaepfer D.D. Participant detection: The colocalization detected by fluorescence patterns were then quantitated on a pixel-by-pixel basis and the Pearson's correlation coefficient was calculated. Some of the coimmunoprecipitations (Figs 1C, 2B, 3A, 5D) and colocalization (Figs 2C, 2D) experiments could not be curated since the specific talin isoform involved in these interactions was not specified and could not be identified. Coimmunoprecipitations (Fig 4A) and colocalizations (Fig 4B, 4C, S2A, S3A, S3B, S3C, S3D) involving GFP-FAK could not be curated since the source of GFP-FAK could not be identified. Direct binding assay (Fig 3E, S2C, S2D) could not be curated since the source of GST-talin could not be identified. dschlaepfer@ucsd.edu Accepted 2013-APR-26 by ORCHARD 8-May-2012: Contacted by IntAct-Help. J. Cell Biol. (0021-9525) 2012 Only protein-protein interactions imex curation tln1_chick Talin-1 TLN1 TLN protein protein chick Gallus gallus (Chicken) Chicken MVALSLKISIGNVVKTMQFEPSTMVYDACRMIRERVPEAQMGQPNDFGLFLSDEDPKKGIWLEAGKALDYYMLRNGDTMEYKKKQRPLKIRMLDGTVKTVMVDDSKTVTDMLTTICARIGITNYDEYSLVREIMEEKKEEVTGTLKKDKTLLRDEKKMEKLKQKLHTDDELNWLDHGRTLREQGIDDNETLLLRRKFFYSDQNVDSRDPVQLNLLYVQARDDILNGSHPVSFDKACEFAGYQCQIQFGPHNEQKHKPGFLELKDFLPKEYIKQKGERKIFMAHKNCGNMSEIEAKVRYVKLARSLKTYGVSFFLVKEKMKGKNKLVPRLLGITKECVMRVDEKTKEVIQEWSLTNIKRWAASPKSFTLDFGDYQDGYYSVQTTEGEQIAQLIAGYIDIILKKKKSKDHFGLEGDEESTMLEDSVSPKKSTVLQQQFNRVGKAELGSVALPAIMRTGAGGPENFQVGTMPQAQMQITSGQMHRGHMPPLTSAQQALTGTINSSMQAVNAAQATLDDFETLPPLGQDAASKAWRKNKMDESKHEIHSQADAITAGTASVVNLTAGDPADTDYTAVGCAVTTISSNLTEMSKGVKLLAALMEDEGGNGRQLLQAAKNLASAVSDLLKTAQPASAEPRQNLLQAAGLVGQTSGELLQQIGESDTDPRFQDMLMQLAKAVASAAAALVLKAKNVAQKTEDSALQTQVIAAATQCALSTSQLVACTKVVAPTISSPVCQEQLIEAGKLVAKSAEGCVEASKAATNDDQLLKQVGVAATAVTQALNDLLQHIKQHATGGQPIGRYDQATDTILNVTENIFSSMGDAGEMVRQARILAQATSDLVNAIKADAEGETDLENSRKLLSAAKILADATAKMVEAAKGAAAHPDSEEQQQRLREAAEGLRMATNAAAQNAIKKKLVHKLEHAAKQAAASATQTIAAAQHAAASNKNPAAQQQLVQSCKVVADQIPMLVQGVRGSQSQPDSPSAQLALIAASQNFLQPGGKMVAAAKATVPTITDQASAMQLSQCAKNLAAALAELRTAAQKAQEACGPLEIDSALGLVQSLERDLKEAKAAARDGKLKPLPGETMEKCAQDLGNSTKAVTSAIAHLLGEVAQGNENYTGIAAREVAQALRSLSQAARGVAANSSDPQAQNAMLECASDVMDKANNLIEEARKAVAKPGDPDSQQRLVQVAKAVSQALNRCVNCLPGQRDVDAAIRMVGEASKRLLSDSFPPSNKTFQEAQSQLNRAAAGLNQSANELVQASRGTPQDLAKSSGKFGQDFNEFLQAGVEMASLSPTKEDQAQVVSNLKSISMSSSKLLLAAKALSADPTSPNLKSQLAAAARAVTDSINQLITMCTQQAPGQKECDNALRELETVKELLENPTQTVNDMSYFSCLDSVMENSKVLGESMAGISQNAKNSKLPEFGESISAASKALCGLTEAAAQAAYLVGVSDPNSQAGQQGLVDPTQFARANQAIQMACQNLVDPACTQSQVLSAATIVAKHTSALCNTCRLASSRTANPVAKRQFVQPAKEVANSTANLVKTIKALDGAFNEENRERCRAATAPLIEAVDNLTAFASNPEFATVPAQISPEGRRAMEPIVTSAKTMLESSAGLIQTARSLAVNPKDPPQWSVLAGHSRTVSDSIKKLITNMRDKAPGQRECDEAIDVLNRCMREVDQASLAAISQQLAPREGISQEALHNQMITAVQEINNLIEPVASAARAEASQLGHKVSQMAQYFEPLILAAIGAASKTPNHQQQMNLLDQTKTLAESALQMLYTAKEAGGNPKQAAHTQEALEEAVQMMKEAVEDLTTTLNEAASAAGVVGGMVDSITQAINQLDEGPMGEPEGTFVDYQTTMVKTAKAIAVTVQEMVTKSTTNPDELGILANQLTNDYGQLAQQAKPAALTAENEEIGSHIKRRVQELGHGCAALVTKAGALQCSPSDAYTKKELIESARKVSEKVSHVLAALQAGNRGTQACITAASAVSGIIADLDTTIMFATAGTLNRENSETFADHREGILKTAKALVEDTKVLVQNATASQEKLAQAAQSSVSTITRLAEVVKLGAASLGSEDPETQVVLINAVKDVAKALGDLIGATKAAAGKAGDDPAVYQLKNSAKVMVTNVTSLLKTVKAVEDEATKGTRALEATIEHIRQELAVFSSPVPPAQVSTPEDFIRMTKGITMATAKAVAAGNSCRQEDVIATANLSRRAIADMLRACKEAAYHPEVSADVRQRALRFGKECADGYLELLEHVLVILQKPTHELKQQLAGYSKRVASSVTELIQAAEAMKGTEWVDPEDPTVIAENELLGAAAAIEAAAKKLEQLKPRAKPKQADESLDFEEQILEAAKSIAAATSALVKAASAAQRELVAQGKVGVIPANAVDDGQWSQGLISAARMVAAATNNLCEAANAAVQGHASEEKLISSAKQVAASTAQLLVACKVKADHDSEAMKRLQAAGNAVKRASDNLVKAAQKAAAFQDHDETVVVKEKMVGGIAQIIAAQEEMLRKERELEEARKKLAMIRQQQYKFLPTELRDEEQN DIP protein P54939 original sequence version: 68 5A94C290C624699E paxi_human Paxillin PXN protein protein human Homo sapiens Human MDDLDALLADLESTTSHISKRPVFLSEETPYSYPTGNHTYQEIAVPPPVPPPPSSEALNGTILDPLDQWQPSSSRFIHQQPQSSSPVYGSSAKTSSVSNPQDSVGSPCSRVGEEEHVYSFPNKQKSAEPSPTVMSTSLGSNLSELDRLLLELNAVQHNPPGFPADEANSSPPLPGALSPLYGVPETNSPLGGKAGPLTKEKPKRNGGRGLEDVRPSVESLLDELESSVPSPVPAITVNQGEMSSPQRVTSTQQQTRISASSATRELDELMASLSDFKIQGLEQRADGERCWAAGWPRDGGRSSPGGQDEGGFMAQGKTGSSSPPGGPPKPGSQLDSMLGSLQSDLNKLGVATVAKGVCGACKKPIAGQVVTAMGKTWHPEHFVCTHCQEEIGSRNFFERDGQPYCEKDYHNLFSPRCYYCNGPILDKVVTALDRTWHPEHFFCAQCGAFFGPEGFHEKDGKAYCRKDYFDMFAPKCGGCARAILENYISALNTLWHPECFVCRECFTPFVNGSFFEHDGQPYCEVHYHERRGSLCSGCQKPITGRCITAMAKKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCQNCFLKLFC DIP protein P49023 original sequence version: 115 DIP protein P49023 original sequence version: 126 DIP protein P49023 original sequence version: 169 DIP protein P49023 original sequence version: 140 DIP protein P49023 original sequence version: 175 ABF6C0BE5939623F fak1_human Focal adhesion kinase 1 PTK2 FAK FAK1 pp125FAK Protein-tyrosine kinase 2 Focal adhesion kinase-related nonkinase Protein phosphatase 1 regulatory subunit 71 p125FAK protein protein human Homo sapiens Human MAAAYLDPNLNHTPNSSTKTHLGTGMERSPGAMERVLKVFHYFESNSEPTTWASIIRHGDATDVRGIIQKIVDSHKVKHVACYGFRLSHLRSEEVHWLHVDMGVSSVREKYELAHPPEEWKYELRIRYLPKGFLNQFTEDKPTLNFFYQQVKSDYMLEIADQVDQEIALKLGCLEIRRSYWEMRGNALEKKSNYEVLEKDVGLKRFFPKSLLDSVKAKTLRKLIQQTFRQFANLNREESILKFFEILSPVYRFDKECFKCALGSSWIISVELAIGPEEGISYLTDKGCNPTHLADFTQVQTIQYSNSEDKDRKGMLQLKIAGAPEPLTVTAPSLTIAENMADLIDGYCRLVNGTSQSFIIRPQKEGERALPSIPKLANSEKQGMRTHAVSVSETDDYAEIIDEEDTYTMPSTRDYEIQRERIELGRCIGEGQFGDVHQGIYMSPENPALAVAIKTCKNCTSDSVREKFLQEALTMRQFDHPHIVKLIGVITENPVWIIMELCTLGELRSFLQVRKYSLDLASLILYAYQLSTALAYLESKRFVHRDIAARNVLVSSNDCVKLGDFGLSRYMEDSTYYKASKGKLPIKWMAPESINFRRFTSASDVWMFGVCMWEILMHGVKPFQGVKNNDVIGRIENGERLPMPPNCPPTLYSLMTKCWAYDPSRRPRFTELKAQLSTILEEEKAQQEERMRMESRRQATVSWDSGGSDEAPPKPSRPGYPSPRSSEGFYPSPQHMVQTNHYQVSGYPGSHGITAMAGSIYPGQASLLDQTDSWNHRPQEIAMWQPNVEDSTVLDLRGIGQVLPTHLMEERLIRQQQEMEEDQRWLEKEERFLKPDVRLSRGSIDREDGSLQGPIGNQHIYQPVGKPDPAAPPKKPPRPGAPGHLGSLASLSSPADSYNEGVKLQPQEISPPPTANLDRSNDKVYENVTGLVKAVIEMSSKIQPAPPEEYVPMVKEVGLALRTLLATVDETIPLLPASTHREIEMAQKLLNSDLGELINKMKLAQQYVMTSLQQEYKKQMLTAAHALAVDAKNLLDVIDQARLKMLGQTRPH D8A4C15138AB0243 ptk2-pxn-7 3 6 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig 1F, 1G. FAK colocalization with paxillin was unaffected in HUVEC cells knocked down for talin. ptk2-pxn-9 2 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c-terminal region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role prey prey physical association physical association Fig 3A (middle panel). ptk2-tln1-1 1 4 unspecified role unspecified role prey prey ferm f2-f3 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain The sequence range [196-400] includes the F2 and F3 regions of the FERM domain. P54939:p.Trp359Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain W A Wed May 02 19:31:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp359ala' Tue Jun 30 16:01:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp359Ala' region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P54939:p.Ala360Glu mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain A E Wed May 02 17:12:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala360glu' Tue Jun 30 16:40:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala360Glu' 6 unspecified role unspecified role bait bait Q05397:p.Glu1015Ala mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A Wed May 02 16:41:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu1015ala' Tue Jun 30 16:23:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu1015Ala' c-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 23 17 physical association physical association Fig 3B, 3C, S2F.