IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Mutations in LRRK2 increase phosphorylation of peroxiredoxin 3 exacerbating oxidative stress-induced neuronal death. Mutations in LRRK2 increase phosphorylation of peroxiredoxin 3 exacerbating oxidative stress-induced neuronal death. Hum. Mutat. (1059-7794) 2011 imex curation Angeles D.C., Gan B.H., Onstead L., Zhao Y., Lim K.L., Dachsel J., Melrose H., Farrer M., Wszolek Z.K., Dickson D.W., Tan E.K. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease I cannot make out most detail on the image - after magnifying page to X400 (power point) - so have taken info entered from text only. (this also means I cannot work out the "expressed-ins" -probably E.coli....) I think it says "sx" next to LRRK2 - short form - so have entered this as a "sufficient to bind" feature. LRRK2 constructs received from matthew j farrer have been entered as Human in origin, -as used in his previous work. The construct pSG5-PRDX3 (contains PRDX3) is murine in origin - as shown by previous publications by Chi V. Dang ( see pmid:12011429). PRDX3 cDNA was shown to be Human in origin by Blatting, and PRDX3 as used in Kinase assays was Human as shown by mutation mapping. gnrtek@sgh.com.sg 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions human-brain-cortex Homo sapiens brain cortex brain_cortex Brain cortex [Cerebral cortex; Cerebrum cortex; Cortex; Pallium] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Angeles D.C., Gan B.H., Onstead L., Zhao Y., Lim K.L., Dachsel J., Melrose H., Farrer M., Wszolek Z.K., Dickson D.W., Tan E.K. Parkinsons - Interactions investigated in the context of Parkinsons disease I cannot make out most detail on the image - after magnifying page to X400 (power point) - so have taken info entered from text only. (this also means I cannot work out the "expressed-ins" -probably E.coli....) I think it says "sx" next to LRRK2 - short form - so have entered this as a "sufficient to bind" feature. LRRK2 constructs received from matthew j farrer have been entered as Human in origin, -as used in his previous work. The construct pSG5-PRDX3 (contains PRDX3) is murine in origin - as shown by previous publications by Chi V. Dang ( see pmid:12011429). PRDX3 cDNA was shown to be Human in origin by Blatting, and PRDX3 as used in Kinase assays was Human as shown by mutation mapping. gnrtek@sgh.com.sg Accepted 2013-SEP-03 by ORCHARD 30-Sept-2013: Contacted by IntAct-Help. Hum. Mutat. (1059-7794) 2011 Only protein-protein interactions imex curation Mutations in LRRK2 increase phosphorylation of peroxiredoxin 3 exacerbating oxidative stress-induced neuronal death. Mutations in LRRK2 increase phosphorylation of peroxiredoxin 3 exacerbating oxidative stress-induced neuronal death. Hum. Mutat. (1059-7794) 2011 imex curation Angeles D.C., Gan B.H., Onstead L., Zhao Y., Lim K.L., Dachsel J., Melrose H., Farrer M., Wszolek Z.K., Dickson D.W., Tan E.K. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease I cannot make out most detail on the image - after magnifying page to X400 (power point) - so have taken info entered from text only. (this also means I cannot work out the "expressed-ins" -probably E.coli....) I think it says "sx" next to LRRK2 - short form - so have entered this as a "sufficient to bind" feature. LRRK2 constructs received from matthew j farrer have been entered as Human in origin, -as used in his previous work. The construct pSG5-PRDX3 (contains PRDX3) is murine in origin - as shown by previous publications by Chi V. Dang ( see pmid:12011429). PRDX3 cDNA was shown to be Human in origin by Blatting, and PRDX3 as used in Kinase assays was Human as shown by mutation mapping. gnrtek@sgh.com.sg 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Angeles D.C., Gan B.H., Onstead L., Zhao Y., Lim K.L., Dachsel J., Melrose H., Farrer M., Wszolek Z.K., Dickson D.W., Tan E.K. Parkinsons - Interactions investigated in the context of Parkinsons disease I cannot make out most detail on the image - after magnifying page to X400 (power point) - so have taken info entered from text only. (this also means I cannot work out the "expressed-ins" -probably E.coli....) I think it says "sx" next to LRRK2 - short form - so have entered this as a "sufficient to bind" feature. LRRK2 constructs received from matthew j farrer have been entered as Human in origin, -as used in his previous work. The construct pSG5-PRDX3 (contains PRDX3) is murine in origin - as shown by previous publications by Chi V. Dang ( see pmid:12011429). PRDX3 cDNA was shown to be Human in origin by Blatting, and PRDX3 as used in Kinase assays was Human as shown by mutation mapping. gnrtek@sgh.com.sg Accepted 2013-SEP-03 by ORCHARD 30-Sept-2013: Contacted by IntAct-Help. Hum. Mutat. (1059-7794) 2011 Only protein-protein interactions imex curation Mutations in LRRK2 increase phosphorylation of peroxiredoxin 3 exacerbating oxidative stress-induced neuronal death. Mutations in LRRK2 increase phosphorylation of peroxiredoxin 3 exacerbating oxidative stress-induced neuronal death. Hum. Mutat. (1059-7794) 2011 imex curation Angeles D.C., Gan B.H., Onstead L., Zhao Y., Lim K.L., Dachsel J., Melrose H., Farrer M., Wszolek Z.K., Dickson D.W., Tan E.K. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease I cannot make out most detail on the image - after magnifying page to X400 (power point) - so have taken info entered from text only. (this also means I cannot work out the "expressed-ins" -probably E.coli....) I think it says "sx" next to LRRK2 - short form - so have entered this as a "sufficient to bind" feature. LRRK2 constructs received from matthew j farrer have been entered as Human in origin, -as used in his previous work. The construct pSG5-PRDX3 (contains PRDX3) is murine in origin - as shown by previous publications by Chi V. Dang ( see pmid:12011429). PRDX3 cDNA was shown to be Human in origin by Blatting, and PRDX3 as used in Kinase assays was Human as shown by mutation mapping. gnrtek@sgh.com.sg 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Angeles D.C., Gan B.H., Onstead L., Zhao Y., Lim K.L., Dachsel J., Melrose H., Farrer M., Wszolek Z.K., Dickson D.W., Tan E.K. Parkinsons - Interactions investigated in the context of Parkinsons disease I cannot make out most detail on the image - after magnifying page to X400 (power point) - so have taken info entered from text only. (this also means I cannot work out the "expressed-ins" -probably E.coli....) I think it says "sx" next to LRRK2 - short form - so have entered this as a "sufficient to bind" feature. LRRK2 constructs received from matthew j farrer have been entered as Human in origin, -as used in his previous work. The construct pSG5-PRDX3 (contains PRDX3) is murine in origin - as shown by previous publications by Chi V. Dang ( see pmid:12011429). PRDX3 cDNA was shown to be Human in origin by Blatting, and PRDX3 as used in Kinase assays was Human as shown by mutation mapping. gnrtek@sgh.com.sg Accepted 2013-SEP-03 by ORCHARD 30-Sept-2013: Contacted by IntAct-Help. human brain cDNA library Hum. Mutat. (1059-7794) 2011 Only protein-protein interactions imex curation Mutations in LRRK2 increase phosphorylation of peroxiredoxin 3 exacerbating oxidative stress-induced neuronal death. Mutations in LRRK2 increase phosphorylation of peroxiredoxin 3 exacerbating oxidative stress-induced neuronal death. Hum. Mutat. (1059-7794) 2011 imex curation Angeles D.C., Gan B.H., Onstead L., Zhao Y., Lim K.L., Dachsel J., Melrose H., Farrer M., Wszolek Z.K., Dickson D.W., Tan E.K. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease I cannot make out most detail on the image - after magnifying page to X400 (power point) - so have taken info entered from text only. (this also means I cannot work out the "expressed-ins" -probably E.coli....) I think it says "sx" next to LRRK2 - short form - so have entered this as a "sufficient to bind" feature. LRRK2 constructs received from matthew j farrer have been entered as Human in origin, -as used in his previous work. The construct pSG5-PRDX3 (contains PRDX3) is murine in origin - as shown by previous publications by Chi V. Dang ( see pmid:12011429). PRDX3 cDNA was shown to be Human in origin by Blatting, and PRDX3 as used in Kinase assays was Human as shown by mutation mapping. gnrtek@sgh.com.sg 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions human-sh_sy5y Homo sapiens SH-SY5Y cell line sh_sy5y Human neuroblastoma cells anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Angeles D.C., Gan B.H., Onstead L., Zhao Y., Lim K.L., Dachsel J., Melrose H., Farrer M., Wszolek Z.K., Dickson D.W., Tan E.K. Parkinsons - Interactions investigated in the context of Parkinsons disease I cannot make out most detail on the image - after magnifying page to X400 (power point) - so have taken info entered from text only. (this also means I cannot work out the "expressed-ins" -probably E.coli....) I think it says "sx" next to LRRK2 - short form - so have entered this as a "sufficient to bind" feature. LRRK2 constructs received from matthew j farrer have been entered as Human in origin, -as used in his previous work. The construct pSG5-PRDX3 (contains PRDX3) is murine in origin - as shown by previous publications by Chi V. Dang ( see pmid:12011429). PRDX3 cDNA was shown to be Human in origin by Blatting, and PRDX3 as used in Kinase assays was Human as shown by mutation mapping. gnrtek@sgh.com.sg Accepted 2013-SEP-03 by ORCHARD 30-Sept-2013: Contacted by IntAct-Help. human brain cDNA library Hum. Mutat. (1059-7794) 2011 Only protein-protein interactions imex curation Mutations in LRRK2 increase phosphorylation of peroxiredoxin 3 exacerbating oxidative stress-induced neuronal death. Mutations in LRRK2 increase phosphorylation of peroxiredoxin 3 exacerbating oxidative stress-induced neuronal death. Hum. Mutat. (1059-7794) 2011 imex curation Angeles D.C., Gan B.H., Onstead L., Zhao Y., Lim K.L., Dachsel J., Melrose H., Farrer M., Wszolek Z.K., Dickson D.W., Tan E.K. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease I cannot make out most detail on the image - after magnifying page to X400 (power point) - so have taken info entered from text only. (this also means I cannot work out the "expressed-ins" -probably E.coli....) I think it says "sx" next to LRRK2 - short form - so have entered this as a "sufficient to bind" feature. LRRK2 constructs received from matthew j farrer have been entered as Human in origin, -as used in his previous work. The construct pSG5-PRDX3 (contains PRDX3) is murine in origin - as shown by previous publications by Chi V. Dang ( see pmid:12011429). PRDX3 cDNA was shown to be Human in origin by Blatting, and PRDX3 as used in Kinase assays was Human as shown by mutation mapping. gnrtek@sgh.com.sg 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip tag visualisation tag visualisation Angeles D.C., Gan B.H., Onstead L., Zhao Y., Lim K.L., Dachsel J., Melrose H., Farrer M., Wszolek Z.K., Dickson D.W., Tan E.K. Parkinsons - Interactions investigated in the context of Parkinsons disease I cannot make out most detail on the image - after magnifying page to X400 (power point) - so have taken info entered from text only. (this also means I cannot work out the "expressed-ins" -probably E.coli....) I think it says "sx" next to LRRK2 - short form - so have entered this as a "sufficient to bind" feature. LRRK2 constructs received from matthew j farrer have been entered as Human in origin, -as used in his previous work. The construct pSG5-PRDX3 (contains PRDX3) is murine in origin - as shown by previous publications by Chi V. Dang ( see pmid:12011429). PRDX3 cDNA was shown to be Human in origin by Blatting, and PRDX3 as used in Kinase assays was Human as shown by mutation mapping. gnrtek@sgh.com.sg Accepted 2013-SEP-03 by ORCHARD 30-Sept-2013: Contacted by IntAct-Help. human brain cDNA library Hum. Mutat. (1059-7794) 2011 Only protein-protein interactions imex curation Mutations in LRRK2 increase phosphorylation of peroxiredoxin 3 exacerbating oxidative stress-induced neuronal death. Mutations in LRRK2 increase phosphorylation of peroxiredoxin 3 exacerbating oxidative stress-induced neuronal death. Hum. Mutat. (1059-7794) 2011 imex curation Angeles D.C., Gan B.H., Onstead L., Zhao Y., Lim K.L., Dachsel J., Melrose H., Farrer M., Wszolek Z.K., Dickson D.W., Tan E.K. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease I cannot make out most detail on the image - after magnifying page to X400 (power point) - so have taken info entered from text only. (this also means I cannot work out the "expressed-ins" -probably E.coli....) I think it says "sx" next to LRRK2 - short form - so have entered this as a "sufficient to bind" feature. LRRK2 constructs received from matthew j farrer have been entered as Human in origin, -as used in his previous work. The construct pSG5-PRDX3 (contains PRDX3) is murine in origin - as shown by previous publications by Chi V. Dang ( see pmid:12011429). PRDX3 cDNA was shown to be Human in origin by Blatting, and PRDX3 as used in Kinase assays was Human as shown by mutation mapping. gnrtek@sgh.com.sg 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions human-sh_sy5y Homo sapiens SH-SY5Y cell line sh_sy5y Human neuroblastoma cells confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Angeles D.C., Gan B.H., Onstead L., Zhao Y., Lim K.L., Dachsel J., Melrose H., Farrer M., Wszolek Z.K., Dickson D.W., Tan E.K. Parkinsons - Interactions investigated in the context of Parkinsons disease I cannot make out most detail on the image - after magnifying page to X400 (power point) - so have taken info entered from text only. (this also means I cannot work out the "expressed-ins" -probably E.coli....) I think it says "sx" next to LRRK2 - short form - so have entered this as a "sufficient to bind" feature. LRRK2 constructs received from matthew j farrer have been entered as Human in origin, -as used in his previous work. The construct pSG5-PRDX3 (contains PRDX3) is murine in origin - as shown by previous publications by Chi V. Dang ( see pmid:12011429). PRDX3 cDNA was shown to be Human in origin by Blatting, and PRDX3 as used in Kinase assays was Human as shown by mutation mapping. gnrtek@sgh.com.sg Accepted 2013-SEP-03 by ORCHARD 30-Sept-2013: Contacted by IntAct-Help. Hum. Mutat. (1059-7794) 2011 Only protein-protein interactions imex curation Mutations in LRRK2 increase phosphorylation of peroxiredoxin 3 exacerbating oxidative stress-induced neuronal death. Mutations in LRRK2 increase phosphorylation of peroxiredoxin 3 exacerbating oxidative stress-induced neuronal death. Hum. Mutat. (1059-7794) 2011 imex curation Angeles D.C., Gan B.H., Onstead L., Zhao Y., Lim K.L., Dachsel J., Melrose H., Farrer M., Wszolek Z.K., Dickson D.W., Tan E.K. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease I cannot make out most detail on the image - after magnifying page to X400 (power point) - so have taken info entered from text only. (this also means I cannot work out the "expressed-ins" -probably E.coli....) I think it says "sx" next to LRRK2 - short form - so have entered this as a "sufficient to bind" feature. LRRK2 constructs received from matthew j farrer have been entered as Human in origin, -as used in his previous work. The construct pSG5-PRDX3 (contains PRDX3) is murine in origin - as shown by previous publications by Chi V. Dang ( see pmid:12011429). PRDX3 cDNA was shown to be Human in origin by Blatting, and PRDX3 as used in Kinase assays was Human as shown by mutation mapping. gnrtek@sgh.com.sg 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro oxidoreduct assay oxidoreductase assay oxidoreduct assay predetermined predetermined participant predetermined Angeles D.C., Gan B.H., Onstead L., Zhao Y., Lim K.L., Dachsel J., Melrose H., Farrer M., Wszolek Z.K., Dickson D.W., Tan E.K. Parkinsons - Interactions investigated in the context of Parkinsons disease I cannot make out most detail on the image - after magnifying page to X400 (power point) - so have taken info entered from text only. (this also means I cannot work out the "expressed-ins" -probably E.coli....) I think it says "sx" next to LRRK2 - short form - so have entered this as a "sufficient to bind" feature. LRRK2 constructs received from matthew j farrer have been entered as Human in origin, -as used in his previous work. The construct pSG5-PRDX3 (contains PRDX3) is murine in origin - as shown by previous publications by Chi V. Dang ( see pmid:12011429). PRDX3 cDNA was shown to be Human in origin by Blatting, and PRDX3 as used in Kinase assays was Human as shown by mutation mapping. gnrtek@sgh.com.sg Accepted 2013-SEP-03 by ORCHARD 30-Sept-2013: Contacted by IntAct-Help. Hum. Mutat. (1059-7794) 2011 Only protein-protein interactions imex curation Mutations in LRRK2 increase phosphorylation of peroxiredoxin 3 exacerbating oxidative stress-induced neuronal death. Mutations in LRRK2 increase phosphorylation of peroxiredoxin 3 exacerbating oxidative stress-induced neuronal death. Hum. Mutat. (1059-7794) 2011 imex curation Angeles D.C., Gan B.H., Onstead L., Zhao Y., Lim K.L., Dachsel J., Melrose H., Farrer M., Wszolek Z.K., Dickson D.W., Tan E.K. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease I cannot make out most detail on the image - after magnifying page to X400 (power point) - so have taken info entered from text only. (this also means I cannot work out the "expressed-ins" -probably E.coli....) I think it says "sx" next to LRRK2 - short form - so have entered this as a "sufficient to bind" feature. LRRK2 constructs received from matthew j farrer have been entered as Human in origin, -as used in his previous work. The construct pSG5-PRDX3 (contains PRDX3) is murine in origin - as shown by previous publications by Chi V. Dang ( see pmid:12011429). PRDX3 cDNA was shown to be Human in origin by Blatting, and PRDX3 as used in Kinase assays was Human as shown by mutation mapping. gnrtek@sgh.com.sg 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions human-chp212 Human CHP212 Neuroblastoma cell line chp212 Human neuroblastoma cell line https://www.lgcstandards-atcc.org/products/all/CRL-2273 confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Angeles D.C., Gan B.H., Onstead L., Zhao Y., Lim K.L., Dachsel J., Melrose H., Farrer M., Wszolek Z.K., Dickson D.W., Tan E.K. Parkinsons - Interactions investigated in the context of Parkinsons disease I cannot make out most detail on the image - after magnifying page to X400 (power point) - so have taken info entered from text only. (this also means I cannot work out the "expressed-ins" -probably E.coli....) I think it says "sx" next to LRRK2 - short form - so have entered this as a "sufficient to bind" feature. LRRK2 constructs received from matthew j farrer have been entered as Human in origin, -as used in his previous work. The construct pSG5-PRDX3 (contains PRDX3) is murine in origin - as shown by previous publications by Chi V. Dang ( see pmid:12011429). PRDX3 cDNA was shown to be Human in origin by Blatting, and PRDX3 as used in Kinase assays was Human as shown by mutation mapping. gnrtek@sgh.com.sg Accepted 2013-SEP-03 by ORCHARD 30-Sept-2013: Contacted by IntAct-Help. Hum. Mutat. (1059-7794) 2011 Only protein-protein interactions imex curation prdx3_human Thioredoxin-dependent peroxide reductase, mitochondrial Peroxiredoxin-3 Peroxiredoxin III Antioxidant protein 1 Protein MER5 homolog HBC189 PRDX3 AOP1 Thioredoxin-dependent peroxiredoxin 3 protein protein human Homo sapiens Human MAAAVGRLLRASVARHVSAIPWGISATAALRPAACGRTSLTNLLCSGSSQAKLFSTSSSCHAPAVTQHAPYFKGTAVVNGEFKDLSLDDFKGKYLVLFFYPLDFTFVCPTEIVAFSDKANEFHDVNCEVVAVSVDSHFSHLAWINTPRKNGGLGHMNIALLSDLTKQISRDYGVLLEGSGLALRGLFIIDPNGVIKHLSVNDLPVGRSVEETLRLVKAFQYVETHGEVCPANWTPDSPTIKPSPAASKEYFQKVNQ DIP protein P30048 original sequence version: 182 8BEB7F5E55BFE9BE hydrogen peroxide hydrogen peroxide [OH(OH)] dihydrogen dioxide H2O2 HOOH Hydrogen peroxide HYDROGEN PEROXIDE Oxydol bis(hydridooxygen)(O--O) dihydrogen peroxide dihydrogen(peroxide) dioxidane hydrogen peroxide small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) MHAJPDPJQMAIIY-UHFFFAOYSA-N MHAJPDPJQMAIIY-UHFFFAOYSA-N prdx3_mouse Thioredoxin-dependent peroxide reductase, mitochondrial Prdx3 Aop1 Mer5 Perioredoxin-3 PRX III Antioxidant protein 1 Protein MER5 Thioredoxin-dependent peroxiredoxin 3 protein protein mouse Mus musculus Mouse MAAAAGRLLWSSVARHASAISRSISASTVLRPVASRRTCLTDILWSASAQGKSAFSTSSSFHTPAVTQHAPYFKGTAVVNGEFKELSLDDFKGKYLVLFFYPLDFTFVCPTEIVAFSDKANEFHDVNCEVVAVSVDSHFSHLAWINTPRKNGGLGHMNITLLSDITKQISRDYGVLLESAGIALRGLFIIDPNGVVKHLSVNDLPVGRSVEETLRLVKAFQFVETHGEVCPANWTPESPTIKPSPTASKEYFEKVHQ 66513F2C5F1D56C0 lrrk2_human Leucine-rich repeat serine/threonine-protein kinase 2 LRRK2 PARK8 Dardarin protein protein human Homo sapiens Human MASGSCQGCEEDEETLKKLIVRLNNVQEGKQIETLVQILEDLLVFTYSERASKLFQGKNIHVPLLIVLDSYMRVASVQQVGWSLLCKLIEVCPGTMQSLMGPQDVGNDWEVLGVHQLILKMLTVHNASVNLSVIGLKTLDLLLTSGKITLLILDEESDIFMLIFDAMHSFPANDEVQKLGCKALHVLFERVSEEQLTEFVENKDYMILLSALTNFKDEEEIVLHVLHCLHSLAIPCNNVEVLMSGNVRCYNIVVEAMKAFPMSERIQEVSCCLLHRLTLGNFFNILVLNEVHEFVVKAVQQYPENAALQISALSCLALLTETIFLNQDLEEKNENQENDDEGEEDKLFWLEACYKALTWHRKNKHVQEAACWALNNLLMYQNSLHEKIGDEDGHFPAHREVMLSMLMHSSSKEVFQASANALSTLLEQNVNFRKILLSKGIHLNVLELMQKHIHSPEVAESGCKMLNHLFEGSNTSLDIMAAVVPKILTVMKRHETSLPVQLEALRAILHFIVPGMPEESREDTEFHHKLNMVKKQCFKNDIHKLVLAALNRFIGNPGIQKCGLKVISSIVHFPDALEMLSLEGAMDSVLHTLQMYPDDQEIQCLGLSLIGYLITKKNVFIGTGHLLAKILVSSLYRFKDVAEIQTKGFQTILAILKLSASFSKLLVHHSFDLVIFHQMSSNIMEQKDQQFLNLCCKCFAKVAMDDYLKNVMLERACDQNNSIMVECLLLLGADANQAKEGSSLICQVCEKESSPKLVELLLNSGSREQDVRKALTISIGKGDSQIISLLLRRLALDVANNSICLGGFCIGKVEPSWLGPLFPDKTSNLRKQTNIASTLARMVIRYQMKSAVEEGTASGSDGNFSEDVLSKFDEWTFIPDSSMDSVFAQSDDLDSEGSEGSFLVKKKSNSISVGEFYRDAVLQRCSPNLQRHSNSLGPIFDHEDLLKRKRKILSSDDSLRSSKLQSHMRHSDSISSLASEREYITSLDLSANELRDIDALSQKCCISVHLEHLEKLELHQNALTSFPQQLCETLKSLTHLDLHSNKFTSFPSYLLKMSCIANLDVSRNDIGPSVVLDPTVKCPTLKQFNLSYNQLSFVPENLTDVVEKLEQLILEGNKISGICSPLRLKELKILNLSKNHISSLSENFLEACPKVESFSARMNFLAAMPFLPPSMTILKLSQNKFSCIPEAILNLPHLRSLDMSSNDIQYLPGPAHWKSLNLRELLFSHNQISILDLSEKAYLWSRVEKLHLSHNKLKEIPPEIGCLENLTSLDVSYNLELRSFPNEMGKLSKIWDLPLDELHLNFDFKHIGCKAKDIIRFLQQRLKKAVPYNRMKLMIVGNTGSGKTTLLQQLMKTKKSDLGMQSATVGIDVKDWPIQIRDKRKRDLVLNVWDFAGREEFYSTHPHFMTQRALYLAVYDLSKGQAEVDAMKPWLFNIKARASSSPVILVGTHLDVSDEKQRKACMSKITKELLNKRGFPAIRDYHFVNATEESDALAKLRKTIINESLNFKIRDQLVVGQLIPDCYVELEKIILSERKNVPIEFPVIDRKRLLQLVRENQLQLDENELPHAVHFLNESGVLLHFQDPALQLSDLYFVEPKWLCKIMAQILTVKVEGCPKHPKGIISRRDVEKFLSKKRKFPKNYMSQYFKLLEKFQIALPIGEEYLLVPSSLSDHRPVIELPHCENSEIIIRLYEMPYFPMGFWSRLINRLLEISPYMLSGRERALRPNRMYWRQGIYLNWSPEAYCLVGSEVLDNHPESFLKITVPSCRKGCILLGQVVDHIDSLMEEWFPGLLEIDICGEGETLLKKWALYSFNDGEEHQKILLDDLMKKAEEGDLLVNPDQPRLTIPISQIAPDLILADLPRNIMLNNDELEFEQAPEFLLGDGSFGSVYRAAYEGEEVAVKIFNKHTSLRLLRQELVVLCHLHHPSLISLLAAGIRPRMLVMELASKGSLDRLLQQDKASLTRTLQHRIALHVADGLRYLHSAMIIYRDLKPHNVLLFTLYPNAAIIAKIADYGIAQYCCRMGIKTSEGTPGFRAPEVARGNVIYNQQADVYSFGLLLYDILTTGGRIVEGLKFPNEFDELEIQGKLPDPVKEYGCAPWPMVEKLIKQCLKENPQERPTSAQVFDILNSAELVCLTRRILLPKNVIVECMVATHHNSRNASIWLGCGHTDRGQLSFLDLNTEGYTSEEVADSRILCLALVHLPVEKESWIVSGTQSGTLLVINTEDGKKRHTLEKMTDSVTCLYCNSFSKQSKQKNFLLVGTADGKLAIFEDKTVKLKGAAPLKILNIGNVSTPLMCLSESTNSTERNVMWGGCGTKIFSFSNDFTIQKLIETRTSQLFSYAAFSDSNIITVVVDTALYIAKQNSPVVEVWDKKTEKLCGLIDCVHFLREVMVKENKESKHKMSYSGRVKTLCLQKNTALWIGTGGGHILLLDLSTRRLIRVIYNFCNSVRVMMTAQLGSLKNVMLVLGYNRKNTEGTQKQKEIQSCLTVWDINLPHEVQNLEKHIEVRKELAEKMRRTSVE DIP protein Q5S007 original sequence version: 36 DIP protein Q5S007 original sequence version: 68 DIP protein Q5S007 original sequence version: 64 26142A0CECBBC3F4 lrrk2-prdx3 3 9 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait kinase_domain sufficient to bind sufficient binding region sufficient to bind range range range range physical association physical association no figure lrrk2-prdx3-1 5 9 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n6-biotinyl-lysine biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 12 unspecified role unspecified role bait bait kinase_domain sufficient to bind sufficient binding region sufficient to bind range range range range region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n6-biotinyl-lysine biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association 1A prdx3-lrrk2 5 12 unspecified role unspecified role prey prey n6-biotinyl-lysine biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined kinase_domain sufficient to bind sufficient binding region sufficient to bind range range range range in vitro In vitro 9 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n6-biotinyl-lysine biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association 1B lrrk2-prdx3-2 4 9 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 1C lrrk2-prdx3-3 1 9 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait physical association physical association 1D prdx3-lrrk2-1 1 9 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey physical association physical association 1D lrrk2-prdx3-5 2 9 enzyme target enzyme target substrate substrate unspecified role unspecified role P30048:p.Thr146Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain T A Wed May 02 15:16:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr146ala' Tue Jun 30 16:37:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr146Ala' 12 enzyme enzyme unspecified role unspecified role Q5S007:p.Gly2019Ser mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain G S Wed May 02 16:04:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly2019ser' Tue Jun 30 18:18:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly2019Ser' region sufficient to bind sufficient binding region sufficient to bind range range range range gfp_or_myc tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-sh_sy5y Homo sapiens SH-SY5Y cell line sh_sy5y Human neuroblastoma cells physical association physical association 2B/C, supp 2 lrrk2-prdx3-7 2 9 enzyme target enzyme target substrate substrate unspecified role unspecified role 12 enzyme enzyme unspecified role unspecified role gfp_or_myc tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q5S007:p.Gly2019Ser mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain G S Wed May 02 15:40:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly2019ser' Tue Jun 30 17:48:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly2019Ser' region sufficient to bind sufficient binding region sufficient to bind range range range range human-sh_sy5y Homo sapiens SH-SY5Y cell line sh_sy5y Human neuroblastoma cells 9 competitor competitor competitor unspecified role unspecified role spt_146 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association 2D, supp 2 hydrogen_perox-prdx3 7 10 enzyme target enzyme target substrate substrate unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) 9 enzyme enzyme unspecified role unspecified role purified purified human-sh_sy5y Homo sapiens SH-SY5Y cell line sh_sy5y Human neuroblastoma cells redox reaction oxidoreductase activity electron transfer reaction redox reaction 4A peroxidase activity was higher in PRDX3 fraction than the phosphorylated PRDX3 fraction. lrrk2-prdx3-8 4 12 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range range range Q5S007:p.[Gly2019Val;Ile2020Phe] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain G V certain certain sequence position certain certain certain sequence position certain I F Wed May 02 18:52:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly2019val,ile2020phe' Tue Jun 30 17:58:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Gly2019Val;Ile2020Phe]' region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey physical association physical association Supp 1C sxWT insert = 4125 bp of nucleotides starting at a unique restriction site (nt 3634, GenBank accession AY792511.1) - nt 3634 is about amino acid 1211 - as entered. PRDX3AS is an antisense construct not a mutated construct... lrrk2-prdx3-9 6 9 unspecified role unspecified role neutral component neutral component 12 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind range range range range colocalization colocalization Supp 1D lrrk2-1 2 12 putative self putative self putative self putative self Q5S007:p.Gly2019Ser mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain G S Wed May 02 15:22:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly2019ser' Tue Jun 30 20:07:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly2019Ser' region sufficient to bind sufficient binding region sufficient to bind range range range range region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-sh_sy5y Homo sapiens SH-SY5Y cell line sh_sy5y Human neuroblastoma cells physical association physical association true 2Bi lrrk2-prdx3-10 8 12 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind range range range range 11 unspecified role unspecified role neutral component neutral component colocalization colocalization Supp 1D