IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ A novel desmocollin-2 mutation reveals insights into the molecular link between desmosomes and gap junctions. A novel desmocollin-2 mutation reveals insights into the molecular link between desmosomes and gap junctions. Heart Rhythm (1547-5271) 2011 imex curation Gehmlich K., Lambiase P.D., Asimaki A., Ciaccio E.J., Ehler E., Syrris P., Saffitz J.E., McKenna W.J. Cardiac - Interactions involving cardiac related proteins This experiment relates to a negative interaction. I have not included "co-locs" Supp Fig 4 C+D as Plakoglobin in this instance is a "marker" only. PLEASE remove the "cautions" at EXPERIMENT levels for the NEGATIVE interactions - I have left them in, as it makes it easier to see which Expts are negative without having to go into annotations for each..... katja.gehmlich@cardiov.ox.ac.uk 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Gehmlich K., Lambiase P.D., Asimaki A., Ciaccio E.J., Ehler E., Syrris P., Saffitz J.E., McKenna W.J. Cardiac - Interactions involving cardiac related proteins This experiment relates to a negative interaction. I have not included "co-locs" Supp Fig 4 C+D as Plakoglobin in this instance is a "marker" only. PLEASE remove the "cautions" at EXPERIMENT levels for the NEGATIVE interactions - I have left them in, as it makes it easier to see which Expts are negative without having to go into annotations for each..... katja.gehmlich@cardiov.ox.ac.uk Accepted 2013-SEP-09 by PPORRAS 30-Sept-2013: Contacted by IntAct-Help. Heart Rhythm (1547-5271) 2011 Only protein-protein interactions imex curation A novel desmocollin-2 mutation reveals insights into the molecular link between desmosomes and gap junctions. A novel desmocollin-2 mutation reveals insights into the molecular link between desmosomes and gap junctions. Heart Rhythm (1547-5271) 2011 imex curation Gehmlich K., Lambiase P.D., Asimaki A., Ciaccio E.J., Ehler E., Syrris P., Saffitz J.E., McKenna W.J. Cardiac - Interactions involving cardiac related proteins This experiment relates to a negative interaction. I have not included "co-locs" Supp Fig 4 C+D as Plakoglobin in this instance is a "marker" only. PLEASE remove the "cautions" at EXPERIMENT levels for the NEGATIVE interactions - I have left them in, as it makes it easier to see which Expts are negative without having to go into annotations for each..... katja.gehmlich@cardiov.ox.ac.uk 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 pull down pull down anti tag western anti tag western blot anti tag western Gehmlich K., Lambiase P.D., Asimaki A., Ciaccio E.J., Ehler E., Syrris P., Saffitz J.E., McKenna W.J. Cardiac - Interactions involving cardiac related proteins This experiment relates to a negative interaction. I have not included "co-locs" Supp Fig 4 C+D as Plakoglobin in this instance is a "marker" only. PLEASE remove the "cautions" at EXPERIMENT levels for the NEGATIVE interactions - I have left them in, as it makes it easier to see which Expts are negative without having to go into annotations for each..... katja.gehmlich@cardiov.ox.ac.uk Accepted 2013-SEP-09 by PPORRAS 30-Sept-2013: Contacted by IntAct-Help. Heart Rhythm (1547-5271) 2011 Only protein-protein interactions imex curation A novel desmocollin-2 mutation reveals insights into the molecular link between desmosomes and gap junctions. A novel desmocollin-2 mutation reveals insights into the molecular link between desmosomes and gap junctions. Heart Rhythm (1547-5271) 2011 imex curation Gehmlich K., Lambiase P.D., Asimaki A., Ciaccio E.J., Ehler E., Syrris P., Saffitz J.E., McKenna W.J. Cardiac - Interactions involving cardiac related proteins This experiment relates to a negative interaction. I have not included "co-locs" Supp Fig 4 C+D as Plakoglobin in this instance is a "marker" only. PLEASE remove the "cautions" at EXPERIMENT levels for the NEGATIVE interactions - I have left them in, as it makes it easier to see which Expts are negative without having to go into annotations for each..... katja.gehmlich@cardiov.ox.ac.uk 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Gehmlich K., Lambiase P.D., Asimaki A., Ciaccio E.J., Ehler E., Syrris P., Saffitz J.E., McKenna W.J. Cardiac - Interactions involving cardiac related proteins This experiment relates to a negative interaction. I have not included "co-locs" Supp Fig 4 C+D as Plakoglobin in this instance is a "marker" only. PLEASE remove the "cautions" at EXPERIMENT levels for the NEGATIVE interactions - I have left them in, as it makes it easier to see which Expts are negative without having to go into annotations for each..... katja.gehmlich@cardiov.ox.ac.uk Accepted 2013-SEP-09 by PPORRAS 30-Sept-2013: Contacted by IntAct-Help. Heart Rhythm (1547-5271) 2011 Only protein-protein interactions imex curation cxa1_human Gap junction alpha-1 protein GJAL Connexin-43 Gap junction 43 kDa heart protein GJA1 protein protein human Homo sapiens Human MGDWSALGKLLDKVQAYSTAGGKVWLSVLFIFRILLLGTAVESAWGDEQSAFRCNTQQPGCENVCYDKSFPISHVRFWVLQIIFVSVPTLLYLAHVFYVMRKEEKLNKKEEELKVAQTDGVNVDMHLKQIEIKKFKYGIEEHGKVKMRGGLLRTYIISILFKSIFEVAFLLIQWYIYGFSLSAVYTCKRDPCPHQVDCFLSRPTEKTIFIIFMLVVSLVSLALNIIELFYVFFKGVKDRVKGKSDPYHATSGALSPAKDCGSQKYAYFNGCSSPTAPLSPMSPPGYKLVTGDRNNSSCRNYNKQASEQNWANYSAEQNRMGQAGSTISNSHAQPFDFPDDNQNSKKLAAGHELQPLAIVDQRPSSRASSRASSRPRPDDLEI 7DDDAD8040284176 plak_rat Junction plakoglobin Jup protein protein rat Rattus norvegicus (Rat) Rat MEVMNLIEQPIKVTEWQQTYTYDSGIHSGVNTCVPSVSSKGLLDEDDTCGRQYTLKKTTTYTQGVPQSQGDLEYQMSTTARAKRVREAMCPGVSGEDSSLLLATQVEGQTTNLQRLAEPSQLLKSAIVHLINYQDDAELATRALPELTKLLNDEDPVVVTKAAMIVNQLSKKEASRRALMGSPQLVAAVVRTMQNTSDLDTARCTTSILHNLSHHREGLLAIFKSGGIPALVRMLSSPVESVLFYAITTLHNLLLYQEGAKMAVRLADGLQKMVPLLNKNNPKFLAITTDCLQLLAYGNQESKLIILANGGPQGLVQIMRNYSYEKLLWTTSRVLKVLSVCPSNKPAIVEAGGMQALGKHLTSNSPRLVQNCLWTLRNLSDVATKQEGLENVLKILVNQLSVDDVNVLTCATGTLSNLTCNNSKNKTLVTQNSGVEALIHAILRAGDKDDITEPAVCALRHLTSRHPEAEMAQNSVRLNYGIPAIVKLLNQPNQWPLVKATIGLIRNLALCPANHAPLQEAAVIPRLVQLLVKAHQDAQRHVAAGTQQPYTDGVRMEEIVEGCTGALHILARDPMNRMEIFRLNTIPLFVQLLYSSVENIQRVAAGVLCELAQDKEAADAIDAEGASAPLMELLHSRNEGTATYAAAVLFRISEDKNPDYRKRVSVELTNSLFKHDPAAWEAAQSMIPINEPYADDMDATYRPMYSSDVPLDPLDMHMDMDGDYPMDTYSDGLRPPYPAADHMLA BF8673E1B797954D q02487-2 Desmocollin-2 DGIII DSC2 CDHF2 DSC3 Cadherin family member 2 Desmocollin-3 Desmosomal glycoprotein II Desmosomal glycoprotein III protein protein human Homo sapiens Human MEAARPSGSWNGALCRLLLLTLAILIFASDACKNVTLHVPSKLDAEKLVGRVNLKECFTAANLIHSSDPDFQILEDGSVYTTNTILLSSEKRSFTILLSNTENQEKKKIFVFLEHQTKVLKKRHTKEKVLRRAKRRWAPIPCSMLENSLGPFPLFLQQVQSDTAQNYTIYYSIRGPGVDQEPRNLFYVERDTGNLYCTRPVDREQYESFEIIAFATTPDGYTPELPLPLIIKIEDENDNYPIFTEETYTFTIFENCRVGTTVGQVCATDKDEPDTMHTRLKYSIIGQVPPSPTLFSMHPTTGVITTTSSQLDRELIDKYQLKIKVQDMDGQYFGLQTTSTCIINIDDVNDHLPTFTRTSYVTSVEENTVDVEILRVTVEDKDLVNTANWRANYTILKGNENGNFKIVTDAKTNEGVLCVVKPLNYEEKQQMILQIGVVNEAPFSREASPRSAMSTATVTVNVEDQDEGPECNPPIQTVRMKENAEVGTTSNGYKAYDPETRSSSGIRYKKLTDPTGWVTIDENTGSIKVFRSLDREAETIKNGIYNITVLASDQGGRTCTGTLGIILQDVNDNSPFIPKKTVIICKPTMSSAEIVAVDPDEPIHGPPFDFSLESSTSEVQRMWRLKAINDTAARLSYQNDPPFGSYVVPITVRDRLGMSSVTSLDVTLCDCITENDCTHRVDPRIGGGGVQLGKWAILAILLGIALLFCILFTLVCGASGTSKQPKVIPDDLAQQNLIVSNTEAPGDDKVYSANGFTTQTVGASAQGVCGTVGSGIKNGGQETIEMVKGGHQTSESCRGAGHHHTLDSCRGGHTEVDNCRYTYSEWHSFTQPRLGEESIRGHTLIKN A53588B1D490CD8F dsc2-gja1-1 2 4 unspecified role unspecified role prey prey n_terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 6 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range cytoplasmic_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association true 6A -wt dsc2-pkp2-1 1 5 unspecified role unspecified role prey prey rat-heart Rattus norvegicus heart heart Heart [Cardiac; Cardiac tissue] 6 unspecified role unspecified role bait bait cytoplasmic_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) physical association physical association true 5B-2b DSC2b (and the mutant) bound only to PKP2 (Figure 5B). dsc2-gja1-5 3 4 unspecified role unspecified role prey prey n_terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range cytoplasmic_c_terminal_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 6 unspecified role unspecified role bait bait cytoplasmic_region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) physical association physical association true 6C i have added the mutant as a "required to bind" feature.