DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Pathogenic LRRK2 negatively regulates microRNA-mediated translational repression. Pathogenic LRRK2 negatively regulates microRNA-mediated translational repression. Nature (0028-0836) 2010 imex curation Gehrke S., Imai Y., Sokol N., Lu B. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease bingwei@stanford.edu,sgehrke@stanford.edu 8-May-2012: Contacted by IntAct-Help. Only protein-protein interactions drome-dopaminergic_neurons Drosophila melanogaster, dopaminergic neurons dopaminergic_neuron Dopaminergic Neurons confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Gehrke S., Imai Y., Sokol N., Lu B. Parkinsons - Interactions investigated in the context of Parkinsons disease This has already been curated by DIP - but I thought it had not been released - but it had been. dopaminergic neurons in the PAL cluster from fly heads in 3 day-old animals. bingwei@stanford.edu,sgehrke@stanford.edu Accepted 2013-APR-24 by ORCHARD 8-May-2012: Contacted by IntAct-Help. Nature (0028-0836) 2010 imex curation Pathogenic LRRK2 negatively regulates microRNA-mediated translational repression. Pathogenic LRRK2 negatively regulates microRNA-mediated translational repression. Nature (0028-0836) 2010 imex curation Gehrke S., Imai Y., Sokol N., Lu B. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease bingwei@stanford.edu,sgehrke@stanford.edu 8-May-2012: Contacted by IntAct-Help. Only protein-protein interactions drome-head Drosophila melanogaster head anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gehrke S., Imai Y., Sokol N., Lu B. Parkinsons - Interactions investigated in the context of Parkinsons disease This has already been curated by DIP - but I thought it had not been released - but it had been. bingwei@stanford.edu,sgehrke@stanford.edu Accepted 2013-APR-24 by ORCHARD 8-May-2012: Contacted by IntAct-Help. Nature (0028-0836) 2010 imex curation Pathogenic LRRK2 negatively regulates microRNA-mediated translational repression. Pathogenic LRRK2 negatively regulates microRNA-mediated translational repression. Nature (0028-0836) 2010 imex curation Gehrke S., Imai Y., Sokol N., Lu B. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease bingwei@stanford.edu,sgehrke@stanford.edu 8-May-2012: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Gehrke S., Imai Y., Sokol N., Lu B. Parkinsons - Interactions investigated in the context of Parkinsons disease bingwei@stanford.edu,sgehrke@stanford.edu Accepted 2013-APR-24 by ORCHARD 8-May-2012: Contacted by IntAct-Help. Nature (0028-0836) 2010 imex curation Pathogenic LRRK2 negatively regulates microRNA-mediated translational repression. Pathogenic LRRK2 negatively regulates microRNA-mediated translational repression. Nature (0028-0836) 2010 imex curation Gehrke S., Imai Y., Sokol N., Lu B. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease bingwei@stanford.edu,sgehrke@stanford.edu 8-May-2012: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Gehrke S., Imai Y., Sokol N., Lu B. Parkinsons - Interactions investigated in the context of Parkinsons disease This has already been curated by DIP - but I thought it had not been released - but it had been. bingwei@stanford.edu,sgehrke@stanford.edu Accepted 2013-APR-24 by ORCHARD 8-May-2012: Contacted by IntAct-Help. Nature (0028-0836) 2010 imex curation Pathogenic LRRK2 negatively regulates microRNA-mediated translational repression. Pathogenic LRRK2 negatively regulates microRNA-mediated translational repression. Nature (0028-0836) 2010 imex curation Gehrke S., Imai Y., Sokol N., Lu B. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease bingwei@stanford.edu,sgehrke@stanford.edu 8-May-2012: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Gehrke S., Imai Y., Sokol N., Lu B. Parkinsons - Interactions investigated in the context of Parkinsons disease Anti myc antibody and anti-p-Thr37/46 were used to detect h4E-BP1. bingwei@stanford.edu,sgehrke@stanford.edu Accepted 2013-APR-24 by ORCHARD 8-May-2012: Contacted by IntAct-Help. Nature (0028-0836) 2010 imex curation Pathogenic LRRK2 negatively regulates microRNA-mediated translational repression. Pathogenic LRRK2 negatively regulates microRNA-mediated translational repression. Nature (0028-0836) 2010 imex curation Gehrke S., Imai Y., Sokol N., Lu B. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease bingwei@stanford.edu,sgehrke@stanford.edu 8-May-2012: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gehrke S., Imai Y., Sokol N., Lu B. Parkinsons - Interactions investigated in the context of Parkinsons disease No cell line is given for Figure 1D, so the same cell line has been entered as for the previous figure, as no other human cell line is stated to have been used in M+M. bingwei@stanford.edu,sgehrke@stanford.edu Accepted 2013-APR-24 by ORCHARD 8-May-2012: Contacted by IntAct-Help. Nature (0028-0836) 2010 imex curation lrrk2_human Leucine-rich repeat serine/threonine-protein kinase 2 LRRK2 PARK8 Dardarin protein protein human Homo sapiens Human MASGSCQGCEEDEETLKKLIVRLNNVQEGKQIETLVQILEDLLVFTYSERASKLFQGKNIHVPLLIVLDSYMRVASVQQVGWSLLCKLIEVCPGTMQSLMGPQDVGNDWEVLGVHQLILKMLTVHNASVNLSVIGLKTLDLLLTSGKITLLILDEESDIFMLIFDAMHSFPANDEVQKLGCKALHVLFERVSEEQLTEFVENKDYMILLSALTNFKDEEEIVLHVLHCLHSLAIPCNNVEVLMSGNVRCYNIVVEAMKAFPMSERIQEVSCCLLHRLTLGNFFNILVLNEVHEFVVKAVQQYPENAALQISALSCLALLTETIFLNQDLEEKNENQENDDEGEEDKLFWLEACYKALTWHRKNKHVQEAACWALNNLLMYQNSLHEKIGDEDGHFPAHREVMLSMLMHSSSKEVFQASANALSTLLEQNVNFRKILLSKGIHLNVLELMQKHIHSPEVAESGCKMLNHLFEGSNTSLDIMAAVVPKILTVMKRHETSLPVQLEALRAILHFIVPGMPEESREDTEFHHKLNMVKKQCFKNDIHKLVLAALNRFIGNPGIQKCGLKVISSIVHFPDALEMLSLEGAMDSVLHTLQMYPDDQEIQCLGLSLIGYLITKKNVFIGTGHLLAKILVSSLYRFKDVAEIQTKGFQTILAILKLSASFSKLLVHHSFDLVIFHQMSSNIMEQKDQQFLNLCCKCFAKVAMDDYLKNVMLERACDQNNSIMVECLLLLGADANQAKEGSSLICQVCEKESSPKLVELLLNSGSREQDVRKALTISIGKGDSQIISLLLRRLALDVANNSICLGGFCIGKVEPSWLGPLFPDKTSNLRKQTNIASTLARMVIRYQMKSAVEEGTASGSDGNFSEDVLSKFDEWTFIPDSSMDSVFAQSDDLDSEGSEGSFLVKKKSNSISVGEFYRDAVLQRCSPNLQRHSNSLGPIFDHEDLLKRKRKILSSDDSLRSSKLQSHMRHSDSISSLASEREYITSLDLSANELRDIDALSQKCCISVHLEHLEKLELHQNALTSFPQQLCETLKSLTHLDLHSNKFTSFPSYLLKMSCIANLDVSRNDIGPSVVLDPTVKCPTLKQFNLSYNQLSFVPENLTDVVEKLEQLILEGNKISGICSPLRLKELKILNLSKNHISSLSENFLEACPKVESFSARMNFLAAMPFLPPSMTILKLSQNKFSCIPEAILNLPHLRSLDMSSNDIQYLPGPAHWKSLNLRELLFSHNQISILDLSEKAYLWSRVEKLHLSHNKLKEIPPEIGCLENLTSLDVSYNLELRSFPNEMGKLSKIWDLPLDELHLNFDFKHIGCKAKDIIRFLQQRLKKAVPYNRMKLMIVGNTGSGKTTLLQQLMKTKKSDLGMQSATVGIDVKDWPIQIRDKRKRDLVLNVWDFAGREEFYSTHPHFMTQRALYLAVYDLSKGQAEVDAMKPWLFNIKARASSSPVILVGTHLDVSDEKQRKACMSKITKELLNKRGFPAIRDYHFVNATEESDALAKLRKTIINESLNFKIRDQLVVGQLIPDCYVELEKIILSERKNVPIEFPVIDRKRLLQLVRENQLQLDENELPHAVHFLNESGVLLHFQDPALQLSDLYFVEPKWLCKIMAQILTVKVEGCPKHPKGIISRRDVEKFLSKKRKFPKNYMSQYFKLLEKFQIALPIGEEYLLVPSSLSDHRPVIELPHCENSEIIIRLYEMPYFPMGFWSRLINRLLEISPYMLSGRERALRPNRMYWRQGIYLNWSPEAYCLVGSEVLDNHPESFLKITVPSCRKGCILLGQVVDHIDSLMEEWFPGLLEIDICGEGETLLKKWALYSFNDGEEHQKILLDDLMKKAEEGDLLVNPDQPRLTIPISQIAPDLILADLPRNIMLNNDELEFEQAPEFLLGDGSFGSVYRAAYEGEEVAVKIFNKHTSLRLLRQELVVLCHLHHPSLISLLAAGIRPRMLVMELASKGSLDRLLQQDKASLTRTLQHRIALHVADGLRYLHSAMIIYRDLKPHNVLLFTLYPNAAIIAKIADYGIAQYCCRMGIKTSEGTPGFRAPEVARGNVIYNQQADVYSFGLLLYDILTTGGRIVEGLKFPNEFDELEIQGKLPDPVKEYGCAPWPMVEKLIKQCLKENPQERPTSAQVFDILNSAELVCLTRRILLPKNVIVECMVATHHNSRNASIWLGCGHTDRGQLSFLDLNTEGYTSEEVADSRILCLALVHLPVEKESWIVSGTQSGTLLVINTEDGKKRHTLEKMTDSVTCLYCNSFSKQSKQKNFLLVGTADGKLAIFEDKTVKLKGAAPLKILNIGNVSTPLMCLSESTNSTERNVMWGGCGTKIFSFSNDFTIQKLIETRTSQLFSYAAFSDSNIITVVVDTALYIAKQNSPVVEVWDKKTEKLCGLIDCVHFLREVMVKENKESKHKMSYSGRVKTLCLQKNTALWIGTGGGHILLLDLSTRRLIRVIYNFCNSVRVMMTAQLGSLKNVMLVLGYNRKNTEGTQKQKEIQSCLTVWDINLPHEVQNLEKHIEVRKELAEKMRRTSVE DIP protein Q5S007 original sequence version: 36 DIP protein Q5S007 original sequence version: 68 DIP protein Q5S007 original sequence version: 64 26142A0CECBBC3F4 4ebp1_human Eukaryotic translation initiation factor 4E-binding protein 1 Phosphorylated heat- and acid-stable protein regulated by insulin 1 EIF4EBP1 protein protein human Homo sapiens Human MSGGSSCSQTPSRAIPATRRVVLGDGVQLPPGDYSTTPGGTLFSTTPGGTRIIYDRKFLMECRNSPVTKTPPRDLPTIPGVTSPSSDEPPMEASQSHLRNSPEDKRAGGEESQFEMDI DIP protein Q13541 original sequence version: 96 DIP protein Q13541 original sequence version: 103 DIP protein Q13541 original sequence version: 120 DIP protein Q13541 original sequence version: 113 1682A6BA74132966 ago2_human Protein argonaute-2 AGO2 Argonaute RISC catalytic component 2 EIF2C2 Eukaryotic translation initiation factor 2C 2 PAZ Piwi domain protein Protein slicer protein protein human Homo sapiens Human MYSGAGPALAPPAPPPPIQGYAFKPPPRPDFGTSGRTIKLQANFFEMDIPKIDIYHYELDIKPEKCPRRVNREIVEHMVQHFKTQIFGDRKPVFDGRKNLYTAMPLPIGRDKVELEVTLPGEGKDRIFKVSIKWVSCVSLQALHDALSGRLPSVPFETIQALDVVMRHLPSMRYTPVGRSFFTASEGCSNPLGGGREVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFVCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQMKRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISKLMRSASFNTDPYVREFGIMVKDEMTDVTGRVLQPPSILYGGRNKAIATPVQGVWDMRNKQFHTGIEIKVWAIACFAPQRQCTEVHLKSFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDHQALAKAVQVHQDTLRTMYFA DIP protein Q9UKV8 original sequence version: 1(def) DIP protein Q9UKV8 original sequence version: 44 DIP protein Q9UKV8 original sequence version: 60 DIP protein Q9UKV8 original sequence version: 62 DIP protein Q9UKV8 original sequence version: 89 DIP protein Q9UKV8 original sequence version: 85 DIP protein Q9UKV8 original sequence version: 87 DIP protein Q9UKV8 original sequence version: 99 DIP protein Q9UKV8 original sequence version: 117 DIP protein Q9UKV8-1 original sequence version: 117 DIP protein Q9UKV8 original sequence version: 145 DIP protein Q9UKV8 original sequence version: 108 5C8552C43FC81345 q32kd4_drome AGO1 AGO1-RC Ago-1 Ago1 Agp1 Dm Ago1 DmAgo1 Dmel\CG6671 MRE20 ago1 ago1-1 anon-WO0257455.29 argonaute1 dAGO1 dAgo1 l(2)04845 AGO-1 Ago l(2)4845 l(2)k00208 CG6671 Dmel_CG6671 l(2)k08121 dmAgo1 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSTERELAPGGPAQLHPHTLPLTFPDLQMTSTVGIIGKVYESQWTPSPTRPQSPSQAQTSFDTLTSPPAPGSSVNPTAVTSPSAQNVAAGGATVAGAAATAAQVASALGATTGSVTPAIATATPATQPDMPVFTCPRRPNLGREGRPIVLRANHFQVTMPRGYVHHYDINIQPDKCPRKVNREIIETMVHAYSKIFGVLKPVFDGRNNLYTRDPLPIGNERLELEVTLPGEGKDRIFRVTIKWQAQVSLFNLEEALEGRTRQIPYDAILALDVVMRHLPSMTYTPVGRSFFSSPEGYYHPLGGGREVWFGFHQSVRPSQWKMMLNIDVSATAFYKAQPVIDFMCEVLDIRDINEQRKPLTDSQRVKFTKEIKGLKIEITHCGQMRRKYRVCNVTRRPAQMQSFPLQLENGQTVECTVAKYFLDKYRMKLRYPHLPCLQVGQEHKHTYLPLEVCNIVAGQRCIKKLTDMQTSTMIKATARSAPDREREINNLVKRADFNNDSYVQEFGLTISNSMMEVRGRVLPPPKLQYGGRVSTGLTGQQLFPPQNKVSLASPNQGVWDMRGKQFFTGVEIRIWAIACFAPQRTVREDALRNFTQQLQKISNDAGMPIIGQPCFCKYATGPDQVEPMFRYLKITFPGLQLVVVVLPGKTPVYAEVKRVGDTVLGMATQCVQAKNVNKTSPQTLSNLCLKINVKLGGINSILVPSIRPKVFNEPVIFLGADVTHPPAGDNKKPSIAAVVGSMDAHPSRYAATVRVQQHRQEIIQELSSMVRELLIMFYKSTGGYKPHRIILYRDGVSEGQFPHVLQHELTAIREACIKLEPEYRPGITFIVVQKRHHTRLFCAEKKEQSGKSGNIPAGTTVDVGITHPTEFDFYLCSHQGIQGTSRPSHYHVLWDDNHFDSDELQCLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVEKEHDSGEGSHQSGCSEDRTPGAMARAITVHADTKKVMYFA DIP protein Q32KD4 original sequence version: 63 123C95C10ACD442E q9vdj9_drome Non-specific serine/threonine protein kinase Lrrk lrrk Dmel_CG5483 dLRRK2 dLrrk CG5483 CT17358 Dmel\CG5483 LRRK LRRK2 dLRRK Lrrk2 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MEHPKTGTETALEACDYFVDEVIEASSIRDAREEVRQIKHGELRTAVISGDERTVRVLLAALGTERQIIVNMAPSGANTLLFLACQSGYESITQRLLDAGADGRSHAVTKYSPLYAAVHSGHLGIARLMLDHFPELIQQPTVERWLPLHAACINGHIKLLELLISYSYPDYLYQTYRDEEGQWEWRLPFDANAHDVTGQTSLYIASILGNKQLVGVLLKWQLHCRRTLGDSASSVSTPITPTRKRISFGIQAIMSKLHISGESEGPDDLASQESTECQRCPINVNLLCGAARETALLAAVRGGHLDVVQSLLQHGANPNIVAKPVEDHNDPKCCEEIYGLSNVPIAEACKQRSLAMLDLLLKHGARDDNGTAIGMAITCGDEAILSRLLARRVHPDSDYKINKKGLPTPVEVNVFLPSTSNISYSAMFPNNPTIIDWHSMGSSVQLSVVRVPWMVSGVLLLNPKLQSHPRLNEVALTAITRIDFSHNVLTSIPQELFHLVSLRYLNVAQNKITDLPAPIGQTYGCPVLDELFLQDNQLTTLPAAIFHLPALSILDVSNNKLQQLPFDLWRAPKLRELNVAFNLLRDLPVPPMQTSSSLLSLDKLNLQSFEEPPSNKPRNVTQQRLTHRNLWSATLDITDNDMKWQHEQDLGDGKTAGVGSSQLSSLNIANNLFTSIPAALPCLAVNLTRLNMSYNSLRSMGHVTSYPATLKQLDLSHNEISCWPSLPRITESDPHLLCYSCVQLPEGRDDDYKTASSKGSSSSATSFRASVLKSVCRHRRHLRLEALRTLILADNLLTRIQLSTDDATTLFNESEDADWSVVGVNRSKVIFPNLSMLDMTNNCLKEIPASLHELSSLSVLNISGNVNITELPPHLGLLSRLWNLNTRGCLLQEPLRSMIESKKHKTMDIVGYLKSIYEDAQPYARMKLMVVGVAGIGKSTLLDLLRQGAGSGSSSSSHRSRASENHWAKRMGHARSTSRSHRHSSASSANISTVGVDIGTWICEKRKRAPGSHGPVVFRTWDFGGQKEYYATHQYFLSKRSLYLVLWRISDGHKGLAELLQWLGNIQARAPNSPVIIVGTHFDAVGESISPQKAEQLQQLIREKFIAIPDAEKIGLPRVIDSIEISCRTLHNIHLLANIIYDTAMQLRSPGSKEPMLLQKIPASYIALEDIVNVIACNLRAAGRDPVLDGEQYKRLVTEQMRLHNYKSFRDAAELQQATTWCHENGVLLHYDDATLRDYYFLDPQWLCDMLAHVVTVREINPFAPTGVMKLDDLQMLFRSVQVQGNGNRSYIVSLLNKFEVALTWDSRTLLIPSLLPSQEAATPNSGSTVKLSQRSRGRSLGCSVSQEVNLNNLIYEQRSAPSSSSSSASVSQGLRRILLMTYFPSGFWSRLITRILADEQIIEAIRGVYMASQDYADFDLRTSLEQDTQWNLWQTGLALYYGPILIFKIWEVPFQKTERTQPFRTDGNRFKLKQDGIWSDVNLSSSSILEVYFPLYEVNISQEVDDNERQLLAEIRPHMSQVAKLLALTVDHIDLLLEDWYPSLGTRFVHTSEGRFLITRLVLCPRCLWKLQLQQNNEPSDREVPPVGCNRPSRSSRRGAGAYFLHGVGDPGEDGALNVFSAYLNATARRERRSEDSLGAGSDADSGVGPDSAGSSRNTSVDGHPGYHLPDNSNVCYAWMIEECILSVYNQSKISCPVHLEQSMAQLAPDVIFADIPDKHTIPSECIIKGSLLGRGAFGFVFKANCKVRGARSFKPVAMKMLQPVPPGARAKESALMAFKVAVGKWDRDPLQHSCKAYCTARQELAVLLTLKHPNIVPLVGICIKPLALVLELAPLGGLDALLRHYRRSGAHMGPHTFQTLVLQAARAIEYLHRRRIIYRDLKSENVLVWELPQPHTEDSPRNLVHIKIADYGISRQTAPSGAKGFGGTEGFMAPEIIRYNGEEEYTEKVDCFSFGMFIYENISLRQPFEGHESIKECILEGSRPALTQRETQFPTCCLDLMVLCWHEQPRRRPTASQIVSILSAPECIHLLDVVAMPHSEKIVCGVFQSLVGMGDDERCGLELWLPSFGSRIDILDCSPSGSLLQCNSISCSPQPQVAPPKTPENGANSRARSAQRLPKMNMLCCCLVGEAIWMGDVSGNLHAYSTSTYAHLFSYMLDPNIKSAVISLVYMEKIARVAVGTHNGRVFLVDATQMPSNCAFAEGSFVLTEICSGFVLHAACSVVVDGIYELWCGEIAGKINVFPLNENGVSGHQALCHSEEPNLIEDVKVARMCSNESHVFSCLYPGCMVYQWDVISKRIENKLDCSKLLPCSESLQSIAIDEHVNLIKCQISALAAHNSELYIGTTWGCLIVAELHTLRPISVFRPYENEIKSIITLSKDNVPLIATIGRRYRSLISRYVDSAESSTKSSAVSTPTHGAAKSVPPADVDNHIHCLLWRAKHWT A4531E16C103C33F lrrk2-eif2c2-1 3 9 unspecified role unspecified role prey prey n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 7 unspecified role unspecified role bait bait Q5S007:p.[Lys1906Met;Asp1994Ala;Asp2017Ala] mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain D A certain certain sequence position certain certain certain sequence position certain K M certain certain sequence position certain certain certain sequence position certain D A Fri Jul 22 10:54:47 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'asp1994ala,lys1906met,asp2017ala' Wed May 02 15:09:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys1906met,asp1994ala,asp2017ala' Tue Jun 30 20:11:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys1906Met;Asp1994Ala;Asp2017Ala]' Q5S007:p.Gly2019Ser mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain G S Wed May 02 17:35:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly2019ser' Tue Jun 30 16:07:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly2019Ser' physical association physical association 1C, Supp 6A eif2c2-lrrk2-2 6 9 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association 1D ago1-lrrk 2 10 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association 1E lrrk2-ago1 4 7 unspecified role unspecified role bait bait c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 10 unspecified role unspecified role prey prey drome-head Drosophila melanogaster head physical association physical association 1F, supp 6C I cannot see that the 2019 mutant is "increasing" - but it is hard to see anything clearly (crappy western in fig 1F) - text no help. ago1-lrrk2 1 7 unspecified role unspecified role neutral component neutral component c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 10 unspecified role unspecified role neutral component neutral component colocalization colocalization supp 5A co-localisation occurs in cytoplasmic punctate structures. eif2c2-eif4ebp1-1 5 9 unspecified role unspecified role bait bait n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 8 unspecified role unspecified role prey prey n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range thr-37 opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt certain certain sequence position certain certain certain sequence position certain Possible thr-46 opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt certain certain sequence position certain certain certain sequence position certain Possible physical association physical association Supp 10B hLRRK2(G2019S) promotes the association between phospho-4E-BP and hAgo2. eif2c2-lrrk2-1 6 9 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association Supp 10C drosophila LRRK(I1915T) promotes the association of endogenous human phospho-4E-BP and human Ago2.