bhf-ucl Myristoylated Naked2 antagonizes Wnt-beta-catenin activity by degrading Dishevelled-1 at the plasma membrane. Myristoylated Naked2 antagonizes Wnt-beta-catenin activity by degrading Dishevelled-1 at the plasma membrane. J. Biol. Chem. (0021-9258) 2010 imex curation Hu T., Li C., Cao Z., Van Raay T.J., Smith J.G., Willert K., Solnica-Krezel L., Coffey R.J. Cardiac - Interactions involving cardiac related proteins The author has not provided requested information regarding the species of Dvl1 in HA-Dvl1 and ubiquitin in His-Ubiquitin and HA-Ubiquitin. Therefore respective PPIs have not been annotated. robert.coffey@vanderbilt.edu 18-Feb-2014: Contacted by IntAct-Help. Only protein-protein interactions human-caco2 Human Caucasian colon adenocarcinoma caco2 Human Caucasian colon adenocarcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hu T., Li C., Cao Z., Van Raay T.J., Smith J.G., Willert K., Solnica-Krezel L., Coffey R.J. Cardiac - Interactions involving cardiac related proteins The human colorectal cancer cell line, Caco-2 used stably over expresses TGFα to protect NKD2 from proteasomal degradation. The author has not provided requested information regarding the species of Dvl1 in HA-Dvl1 and ubiquitin in His-Ubiquitin and HA-Ubiquitin. Therefore respective PPIs have not been annotated. robert.coffey@vanderbilt.edu. robert.coffey@vanderbilt.edu Accepted 2014-FEB-12 by MGT J. Biol. Chem. (0021-9258) 2010 Only protein-protein interactions imex curation nkd2_human Protein naked cuticle homolog 2 NKD2 protein protein human Homo sapiens Human MGKLQSKHAAAARKRRESPEGDSFVASAYASGRKGAEEAERRARDKQELPNGDPKEGPFREDQCPLQVALPAEKAEGREHPGQLLSADDGERAANREGPRGPGGQRLNIDALQCDVSVEEDDRQEWTFTLYDFDNCGKVTREDMSSLMHTIYEVVDASVNHSSGSSKTLRVKLTVSPEPSSKRKEGPPAGQDREPTRCRMEGELAEEPRVADRRLSAHVRRPSTDPQPCSERGPYCVDENTERRNHYLDLAGIENYTSRFGPGSPPVQAKQEPQGRASHLQARSRSQEPDTHAVHHRRSQVLVEHVVPASEPAARALDTQPRPKGPEKQFLKSPKGSGKPPGVPASSKSGKAFSYYLPAVLPPQAPQDGHHLPQPPPPPYGHKRYRQKGREGHSPLKAPHAQPATVEHEVVRDLPPTPAGEGYAVPVIQRHEHHHHHEHHHHHHHHHFHPS DIP protein Q969F2 original sequence version: 65 F27E708F9FAD1F2A dvl1_human Segment polarity protein dishevelled homolog DVL-1 DVL1 DSH homolog 1 protein protein human Homo sapiens Human MAETKIIYHMDEEETPYLVKLPVAPERVTLADFKNVLSNRPVHAYKFFFKSMDQDFGVVKEEIFDDNAKLPCFNGRVVSWLVLAEGAHSDAGSQGTDSHTDLPPPLERTGGIGDSRPPSFHPNVASSRDGMDNETGTESMVSHRRERARRRNREEAARTNGHPRGDRRRDVGLPPDSASTALSSELESSSFVDSDEDGSTSRLSSSTEQSTSSRLIRKHKRRRRKQRLRQADRASSFSSITDSTMSLNIVTVTLNMERHHFLGISIVGQSNDRGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNDVNFENMSNDDAVRVLREIVSQTGPISLTVAKCWDPTPRSYFTVPRADPVRPIDPAAWLSHTAALTGALPRYGTSPCSSAVTRTSSSSLTSSVPGAPQLEEAPLTVKSDMSAVVRVMQLPDSGLEIRDRMWLKITIANAVIGADVVDWLYTHVEGFKERREARKYASSLLKHGFLRHTVNKITFSEQCYYVFGDLCSNLATLNLNSGSSGTSDQDTLAPLPHPAAPWPLGQGYPYQYPGPPPCFPPAYQDPGFSYGSGSTGSQQSEGSKSSGSTRSSRRAPGREKERRAAGAGGSGSESDHTAPSGVGSSWRERPAGQLSRGSSPRSQASATAPGLPPPHPTTKAYTVVGGPPGGPPVRELAAVPPELTGSRQSFQKAMGNPCEFFVDIM DIP protein O14640 original sequence version: 114 B009BDBCC57BD562 nkd2-dvl1-2 1 2 unspecified role unspecified role bait bait 3 unspecified role unspecified role prey prey 'Only a slower migrating form of DVL-1 interacts with NKD2 and this form represents a minor proportion of total DVL-1'. physical association physical association Fig. 5B, 5C MG132: a specific, potent, reversible, and cell-permeable proteasome inhibitor (Ki = 4 nM) . Reduces the degradation of ubiquitin-conjugated proteins.