IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ TEX14 interacts with CEP55 to block cell abscission. TEX14 interacts with CEP55 to block cell abscission. Mol. Cell. Biol. (0270-7306) 2010 imex curation Iwamori T., Iwamori N., Ma L., Edson M.A., Greenbaum M.P., Matzuk M.M. mmatzuk@bcm.tmc.edu 17-Jun-2013: Contacted by IntAct-Help. Only protein-protein interactions mouse-testis Mus musculus testis testis fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Iwamori T., Iwamori N., Ma L., Edson M.A., Greenbaum M.P., Matzuk M.M. mmatzuk@bcm.tmc.edu Accepted 2013-MAY-31 by ORCHARD 17-Jun-2013: Contacted by IntAct-Help. Mol. Cell. Biol. (0270-7306) 2010 Only protein-protein interactions imex curation TEX14 interacts with CEP55 to block cell abscission. TEX14 interacts with CEP55 to block cell abscission. Mol. Cell. Biol. (0270-7306) 2010 imex curation Iwamori T., Iwamori N., Ma L., Edson M.A., Greenbaum M.P., Matzuk M.M. mmatzuk@bcm.tmc.edu 17-Jun-2013: Contacted by IntAct-Help. Only protein-protein interactions mouse-testis Mus musculus testis testis cosedimentation cosedimentation immunostaining immunostaining Iwamori T., Iwamori N., Ma L., Edson M.A., Greenbaum M.P., Matzuk M.M. mmatzuk@bcm.tmc.edu Accepted 2013-MAY-31 by ORCHARD 17-Jun-2013: Contacted by IntAct-Help. Mol. Cell. Biol. (0270-7306) 2010 Only protein-protein interactions imex curation TEX14 interacts with CEP55 to block cell abscission. TEX14 interacts with CEP55 to block cell abscission. Mol. Cell. Biol. (0270-7306) 2010 imex curation Iwamori T., Iwamori N., Ma L., Edson M.A., Greenbaum M.P., Matzuk M.M. mmatzuk@bcm.tmc.edu 17-Jun-2013: Contacted by IntAct-Help. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Iwamori T., Iwamori N., Ma L., Edson M.A., Greenbaum M.P., Matzuk M.M. mmatzuk@bcm.tmc.edu Accepted 2013-MAY-31 by ORCHARD 17-Jun-2013: Contacted by IntAct-Help. Mol. Cell. Biol. (0270-7306) 2010 Only protein-protein interactions imex curation TEX14 interacts with CEP55 to block cell abscission. TEX14 interacts with CEP55 to block cell abscission. Mol. Cell. Biol. (0270-7306) 2010 imex curation Iwamori T., Iwamori N., Ma L., Edson M.A., Greenbaum M.P., Matzuk M.M. mmatzuk@bcm.tmc.edu 17-Jun-2013: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. gal4 vp16 complement gal4 vp16 complementation karyoplasmic interaction ion strategy KISS mammalian two hybrid gal4 vp16 complement predetermined predetermined participant predetermined Iwamori T., Iwamori N., Ma L., Edson M.A., Greenbaum M.P., Matzuk M.M. mmatzuk@bcm.tmc.edu Accepted 2013-MAY-31 by ORCHARD 17-Jun-2013: Contacted by IntAct-Help. Mol. Cell. Biol. (0270-7306) 2010 Only protein-protein interactions imex curation TEX14 interacts with CEP55 to block cell abscission. TEX14 interacts with CEP55 to block cell abscission. Mol. Cell. Biol. (0270-7306) 2010 imex curation Iwamori T., Iwamori N., Ma L., Edson M.A., Greenbaum M.P., Matzuk M.M. mmatzuk@bcm.tmc.edu 17-Jun-2013: Contacted by IntAct-Help. Only protein-protein interactions mouse-testis Mus musculus testis testis cosedimentation cosedimentation western blot western blot Immuno blot Immuno blot Iwamori T., Iwamori N., Ma L., Edson M.A., Greenbaum M.P., Matzuk M.M. mmatzuk@bcm.tmc.edu Accepted 2013-MAY-31 by ORCHARD 17-Jun-2013: Contacted by IntAct-Help. Mol. Cell. Biol. (0270-7306) 2010 Only protein-protein interactions imex curation TEX14 interacts with CEP55 to block cell abscission. TEX14 interacts with CEP55 to block cell abscission. Mol. Cell. Biol. (0270-7306) 2010 imex curation Iwamori T., Iwamori N., Ma L., Edson M.A., Greenbaum M.P., Matzuk M.M. mmatzuk@bcm.tmc.edu 17-Jun-2013: Contacted by IntAct-Help. Only protein-protein interactions mouse-ovary Mouse ovary ovary Ovary fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Iwamori T., Iwamori N., Ma L., Edson M.A., Greenbaum M.P., Matzuk M.M. mmatzuk@bcm.tmc.edu Accepted 2013-MAY-31 by ORCHARD 17-Jun-2013: Contacted by IntAct-Help. Mol. Cell. Biol. (0270-7306) 2010 Only protein-protein interactions imex curation TEX14 interacts with CEP55 to block cell abscission. TEX14 interacts with CEP55 to block cell abscission. Mol. Cell. Biol. (0270-7306) 2010 imex curation Iwamori T., Iwamori N., Ma L., Edson M.A., Greenbaum M.P., Matzuk M.M. mmatzuk@bcm.tmc.edu 17-Jun-2013: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Iwamori T., Iwamori N., Ma L., Edson M.A., Greenbaum M.P., Matzuk M.M. mmatzuk@bcm.tmc.edu Accepted 2013-MAY-31 by ORCHARD 17-Jun-2013: Contacted by IntAct-Help. Mol. Cell. Biol. (0270-7306) 2010 Only protein-protein interactions imex curation TEX14 interacts with CEP55 to block cell abscission. TEX14 interacts with CEP55 to block cell abscission. Mol. Cell. Biol. (0270-7306) 2010 imex curation Iwamori T., Iwamori N., Ma L., Edson M.A., Greenbaum M.P., Matzuk M.M. mmatzuk@bcm.tmc.edu 17-Jun-2013: Contacted by IntAct-Help. Only protein-protein interactions mouse-testis Mus musculus testis testis cosedimentation cosedimentation ms participant Identification by mass spectrometry ms participant Iwamori T., Iwamori N., Ma L., Edson M.A., Greenbaum M.P., Matzuk M.M. mmatzuk@bcm.tmc.edu Accepted 2013-MAY-31 by ORCHARD 17-Jun-2013: Contacted by IntAct-Help. Mol. Cell. Biol. (0270-7306) 2010 Only protein-protein interactions imex curation cep55_mouse Centrosomal protein of 55 kDa Cep55 protein protein mouse Mus musculus Mouse MSSRSPKDLIKSKWGSRPSSSKSDTALEKFKGEIAAFKTSLDEITSGKGKMAEKGRSRLLEKIQVLEAEREKNVYYLLEKDKEIQRLKDHLRSRYSSSSLFEQLEEKTKECEKKQQLLESLSKETDVLKNQLSATTKRLSELESKASTLHLSQSMPANCFNSSMNSIHEKEMQLKDALEKNQQWLVYDQQREAYVKGLLAKIFELEKRTETAAASLTQQMKKIESEGYLQVEKQKYDHLLENAKKDLEVERQAVTQLRLELDEFRRKYEEARKEVEDLNQLLSSQRKADIQHLEEDKQKTERIQKLREESSIFKGKLEEERKRSEELLSQVRILYDSLLKHQEEQARVALLEQQMQACTLDFENEKLDRQNMQHQLYVILKELRKAKSQITQLESLKQLHGFTITEQPFPLQREPESRVKATSPKSPSAALNDSLVECPKCSVQYPATEHRDLLVHVEYCMK 5D2308BFCEADA08B tex14_mouse Inactive serine/threonine-protein kinase TEX14 Tex14 Testis-expressed sequence 14 Testis-expressed sequence 14 protein protein protein mouse Mus musculus Mouse MSRGAPFPVPCPVLLGTFTDDSLEAQLHEYAKQGNCVKLKKILKKGVCVDAVNTQGQSALFVAALLGHVKLVDVLVDYGSDPNHRCFDGSTPVHAAAFSGNQWILSKLLTAGGDLRLHDEKGRNPQAWALTAGKDRSTQMVEFMQRCTSHMKAIIQGFSYDLLKKIDSPQRLIGSPPWFGSLIQGSPNSSPNRQLKPGIISAQNIYSFGFGKFYLTSGMQLTYPGSLPVIGEKEVVQADDEPTFSFFSGPYMVMTNLVWNRSRVTVKELNLPTRPHCSRLRLADLLIAEQEHSSNLRHPNLLQLMAVCLSRDLEKIRLVYERITVGTLFSVLHERRSQFPVLHMEVIVHLLLQVADALIYLHSRGFIHRSLSSYAVHIVSAGEARLTNLEYLTESQDSGAHRNVTRMPLPTQLYNWAAPEVVLQKAATVKSDIYSFSVIIQEILTDSIPWNGLDGSLVKETIALGNYLEADVRLPEPYYDIVKSGIHAKQKNRTMNLQDIRYILKNDLKEFIGAQKTQPTESPRGQSYEPHPDVNICLGLTSEYQKDPPDLDIKELKEMGSQPHSPTDHSFLTVKPTLAPQTLDSSLSAQKPDNANVPSPPAACLAEEVRSPTASQDSLCSFEINEIYSGCLTLGTDKEEECLGTAASPEGDRPNQGDELPSLEEELDKMERELHCFCEEDKSISEVDTDLLFEDDDWQSDSLGSLNLPEPTREAKGKTSSWSKTDEYVSKCVLNLKISQVMMQQSAEWLRKLEQEVEELEWAQKELDSQCSSLRDASLKFANAKFQPAVGPPSLAYLPPVMQLPGLKQPENGGTWLTLARSPGNEREFQEGHFSKKPEKLSACGWKPFTQVSEESRGDCSELNNQLPTLRGPGKQSTGEQLPSTQEARESLEKNTNQNSRSMASVSSEIYATKSRNNEDNGEAHLKWRLAVKEMAEKAVSGQLLLPPWNPQSSAPFESKVENESTPLPRPPIRGPESTEWQHILEYQRENDEPKGNTKFGKMDNSDCDKNKHSRWTGLQRFTGIRYPFFRNHEQPEQNEASQASCDTSVGTEKFYSTSSPIGDDFERFQDSFAQRQGYVEENFQIREIFEKNAEILTKPQFQAIQCAEDKQDETLGETPKELKEKNTSLTDIQDLSSITYDQDGYFKETSYKTPKLKHAPTSASTPLSPESISSAASHYEDCLENTTFHVKRGSTFCWNGQEAMRTLSAKFTTVRERAKSLESLLASSKSLPAKLTDSKRLCMLSETGSSNVSAAFVTSTHATKRKSLPRELAEATSQQHLDELPPPAQELLDEIEQLKQQQVSSLASHENTARDLSVTNKDKKHLEEQETNSSKDSSFLSSREIQDLEDTERAHSSLDEDLERFLQSPEENTALLDPTKGSTREKKNKDQDVVEQKRKKKESIKPERRESDSSLGTLEEDELKPCFWKRLGWSEPSRIIVLDQSDLSD 0628605EBE0FF0A4 kif23_mouse Kinesin-like protein KIF23 Kif23 protein protein mouse Mus musculus Mouse MKSAKAKTVRKPVIKKGSQTNLKDPVGVYCRVRPLSFPDQECCVEVINSTTLQLHTPEGYRLNRNGDYKETQYSFKRVFGTHTTQKELFDVVANPLVDDLIHGKNGLLFTYGVTGSGKTYTMTGSPGSGGLLPRCLNMIFNSIGSFQAKRYVFKSNDRNSMEIQCEVDALLERQKREALPIPKTPSSKRQADPEFADMINVQEFCKAEEVDEDSVYGVFVSYIEIYNNYIYDLLEEVQFDPIKPKLPQSKTLREDKNHNMYVAGCTEVEVKSTEEAFEVFWRGQKKRRIANTHLNRESSRSHSVFSIKLVQAPLDADGDNVLQEKEQITISQLSLVDLAGSERTNRTKAEGNRLREAGNINQSLMTLRTCMEVLRENQTYGTNKMVPYRDSKLTHLFKNYFDGEGKVRMIVCVNPKAEDYEESLQVMRFAEVTQEVEVARPVDKVICGLTPGRRYRNLPRGGPVGDEPLVPEVILQSFPPLPPCKLLDINDEETLPKLADTLEKRHHLRQLMTEDLNKKCLAFKALLKEFDNSLSNKENYVQEKLNEREKVISGQKLEIERLEKKNKTLEYKIEILEKTTTIYEEDKRNLQQELESQNQKLQRQFSDKRRLEARLQGMVTETSMKWQKECERRVAATQLEMQNKLWVKDEKLKQLKAIVTEPKPEKPERPSRERDREKIIPRSVSPSPLPLSSNNIAQISNGQQLMSQPQLHRRSNSCSSISVASCISEWEQKLSPFSTPVNVTSLARHRQQEPGQSKTCIVSDRRRGMCWTEGREMVPTFSSEIGVEEDHCRRNTPIPVRHRRSRSAGSRWVDHKPASNVQTETVMQPHVPHAITVSVANEKALAKCEKYMLTHQELASDGEIQTKVIKGDVYKTRGGGQSVQFTDIETLKQELPTGSRKRRSSTLAPAQPDGTESEWTDVETRCSVAVEMRAGSQLGPGYQHHAQPKRKKP DE6E050D59A18513 ts101_mouse Tumor susceptibility gene 101 protein Tsg101 ESCRT-I complex subunit TSG101 protein protein mouse Mus musculus Mouse MAVSESQLKKMMSKYKYRDLTVRQTVNVIAMYKDLKPVLDSYVFNDGSSRELVNLTGTIPVRYRGNIYNIPICLWLLDTYPYNPPICFVKPTSSMTIKTGKHVDANGKIYLPYLHDWKHPRSELLELIQIMIVIFGEEPPVFSRPTVSASYPPYTATGPPNTSYMPGMPSGISAYPSGYPPNPSGYPGCPYPPAGPYPATTSSQYPSQPPVTTVGPSRDGTISEDTIRASLISAVSDKLRWRMKEEMDGAQAELNALKRTEEDLKKGHQKLEEMVTRLDQEVAEVDKNIELLKKKDEELSSALEKMENQSENNDIDEVIIPTAPLYKQILNLYAEENAIEDTIFYLGEALRRGVIDLDVFLKHVRLLSRKQFQLRALMQKARKTAGLSDLY 79558EC535093492 ts101_human Tumor susceptibility gene 101 protein TSG101 ESCRT-I complex subunit TSG101 protein protein human Homo sapiens Human MAVSESQLKKMVSKYKYRDLTVRETVNVITLYKDLKPVLDSYVFNDGSSRELMNLTGTIPVPYRGNTYNIPICLWLLDTYPYNPPICFVKPTSSMTIKTGKHVDANGKIYLPYLHEWKHPQSDLLGLIQVMIVVFGDEPPVFSRPISASYPPYQATGPPNTSYMPGMPGGISPYPSGYPPNPSGYPGCPYPPGGPYPATTSSQYPSQPPVTTVGPSRDGTISEDTIRASLISAVSDKLRWRMKEEMDRAQAELNALKRTEEDLKKGHQKLEEMVTRLDQEVAEVDKNIELLKKKDEELSSALEKMENQSENNDIDEVIIPTAPLYKQILNLYAEENAIEDTIFYLGEALRRGVIDLDVFLKHVRLLSRKQFQLRALMQKARKTAGLSDLY ADD6912FC22DF162 pdc6i_human Programmed cell death 6-interacting protein PDCD6IP AIP1 KIAA1375 ALIX ALG-2-interacting protein 1 Hp95 ALG-2-interacting protein X protein protein human Homo sapiens Human MATFISVQLKKTSEVDLAKPLVKFIQQTYPSGGEEQAQYCRAAEELSKLRRAAVGRPLDKHEGALETLLRYYDQICSIEPKFPFSENQICLTFTWKDAFDKGSLFGGSVKLALASLGYEKSCVLFNCAALASQIAAEQNLDNDEGLKIAAKHYQFASGAFLHIKETVLSALSREPTVDISPDTVGTLSLIMLAQAQEVFFLKATRDKMKDAIIAKLANQAADYFGDAFKQCQYKDTLPKEVFPVLAAKHCIMQANAEYHQSILAKQQKKFGEEIARLQHAAELIKTVASRYDEYVNVKDFSDKINRALAAAKKDNDFIYHDRVPDLKDLDPIGKATLVKSTPVNVPISQKFTDLFEKMVPVSVQQSLAAYNQRKADLVNRSIAQMREATTLANGVLASLNLPAAIEDVSGDTVPQSILTKSRSVIEQGGIQTVDQLIKELPELLQRNREILDESLRLLDEEEATDNDLRAKFKERWQRTPSNELYKPLRAEGTNFRTVLDKAVQADGQVKECYQSHRDTIVLLCKPEPELNAAIPSANPAKTMQGSEVVNVLKSLLSNLDEVKKEREGLENDLKSVNFDMTSKFLTALAQDGVINEEALSVTELDRVYGGLTTKVQESLKKQEGLLKNIQVSHQEFSKMKQSNNEANLREEVLKNLATAYDNFVELVANLKEGTKFYNELTEILVRFQNKCSDIVFARKTERDELLKDLQQSIAREPSAPSIPTPAYQSSPAGGHAPTPPTPAPRTMPPTKPQPPARPPPPVLPANRAPSATAPSPVGAGTAAPAPSQTPGSAPPPQAQGPPYPTYPGYPGYCQMPMPMGYNPYAYGQYNMPYPPVYHQSPGQAPYPGPQQPSYPFPQPPQQSYYPQQ DIP protein Q8WUM4 original sequence version: 46 DIP protein Q8WUM4 original sequence version: 67 DIP protein Q8WUM4 original sequence version: 60 DIP protein Q8WUM4 original sequence version: 64 DIP protein Q8WUM4 original sequence version: 66 DIP protein Q8WUM4 original sequence version: 89 DIP protein Q8WUM4 original sequence version: 100 DIP protein Q8WUM4-1 original sequence version: 145 DIP protein Q8WUM4-1 original sequence version: 137 DIP protein Q8WUM4-1 original sequence version: 138 DIP protein Q8WUM4 original sequence version: 138 DIP protein Q8WUM4 original sequence version: 106 573588D1F612EC93 pdc6i_mouse Programmed cell death 6-interacting protein ALG-2-interacting protein X ALG-2-interacting protein 1 E2F1-inducible protein Eig2 Pdcd6ip Aip1 Alix protein protein mouse Mus musculus Mouse MASFIWVQLKKTSEVDLAKPLVKFIQQTYPSGGEEQAQYCRAAEELSKLRRSALGRPLDKHEGALETLLRYYDQICSIEPKFPFSENQICLTFTWKDAFDKGSLFGGSVKLALASLGYEKSCVLFNCAALASQIAAEQNLDNDEGLKTAAKQYQFASGAFLHIKDTVLSALSREPTVDISPDTVGTLSLIMLAQAQEVFFLKATRDKMKDAIIAKLANQAADYFGDAFKQCQYKDTLPKEVFPTLAAKQCIMQANAEYHQSILAKQQKKFGEEIARLQHAAELIKNVASRYDEYVNVKDFSDKINRALTAAKKDNDFIYHDRVPDLKDLDPIGKATLVKPTPVNVPVSQKFTDLFEKMVPVSVQQSLAVFSQRKADLVNRSIAQMREATTLANGVLASLNLPAAIEDVSGDTVPQSILTKSTSVVEQGGIQTVDQLIKELPELLQRNREILEESLRLLDEEEATDNDLRAKFKDRWQRTPSNDLYKPLRAEGAKFRAVLDKAVQADGQVKERYQSHRDTIALLCKPEPELNAAIPSANPAKTMQGSEVVSVLKSLLSNLDEIKKERESLENDLKSVNFDMTSKFLTALAQDGVINEEALSVTELDRIYGGLTSKVQESLKKQEGLLKNIQVSHQEFSKMKQSNNEANLREEVLKNLATAYDNFVELVANLKEGTKFYNELTEILVRFQNKCSDIVFARKTERDELLKDLQQSIAREPSAPSIPPPAYQSSPAAGHAAAPPTPAPRTMPPAKPQPPARPPPPVLPANRVPPASAAAAPAGVGTASAAPPQTPGSAPPPQAQGPPYPTYPGYPGYCQMPMPMGYNPYAYGQYNMPYPPVYHQSPGQAPYPGPQQPTYPFPQPPQQSYYPQQ BFDC87ACA183C5D4 cep55_human Centrosomal protein of 55 kDa Up-regulated in colon cancer 6 CEP55 URCC6 C10orf3 protein protein human Homo sapiens Human MSSRSTKDLIKSKWGSKPSNSKSETTLEKLKGEIAHLKTSVDEITSGKGKLTDKERHRLLEKIRVLEAEKEKNAYQLTEKDKEIQRLRDQLKARYSTTTLLEQLEETTREGERREQVLKALSEEKDVLKQQLSAATSRIAELESKTNTLRLSQTVAPNCFNSSINNIHEMEIQLKDALEKNQQWLVYDQQREVYVKGLLAKIFELEKKTETAAHSLPQQTKKPESEGYLQEEKQKCYNDLLASAKKDLEVERQTITQLSFELSEFRRKYEETQKEVHNLNQLLYSQRRADVQHLEDDRHKTEKIQKLREENDIARGKLEEEKKRSEELLSQVQFLYTSLLKQQEEQTRVALLEQQMQACTLDFENEKLDRQHVQHQLHVILKELRKARNQITQLESLKQLHEFAITEPLVTFQGETENREKVAASPKSPTAALNESLVECPKCNIQYPATEHRDLLVHVEYCSK DIP protein Q53EZ4 original sequence version: 39 A652F86EBCE46A51 tex14_human Inactive serine/threonine-protein kinase TEX14 TEX14 SGK307 Protein kinase-like protein SgK307 Sugen kinase 307 Testis-expressed sequence 14 Testis-expressed sequence 14 protein protein protein human Homo sapiens Human MSRAVRLPVPCPVQLGTLRNDSLEAQLHEYVKQGNYVKVKKILKKGIYVDAVNSLGQTALFVAALLGLRKFVDVLVDYGSDPNHRCFDGSTPVHAAAFSGNQWILSKLLDAGGDLRLHDERGQNPKTWALTAGKERSTQIVEFMQRCASHMQAIIQGFSYDLLKKIDSPQRLVYSPSWCGGLVQGNPNGSPNRLLKAGVISAQNIYSFGFGKAMPWFQFYLTGATQMAYLGSLPVIGEKEVIQADDEPTFSFFSGPYMVMTNLVWNGSRVTVKELNLPTHPHCSRLRLADLLIAEQEHSSKLRHPYLLQLMAVCLSQDLEKTRLVYERITIGTLFSVLHERRSQFPVLHMEVIVHLLLQISDALRYLHFQGFIHRSLSSYAVHIISPGEARLTNLEYMLESEDRGVQRDLTRVPLPTQLYNWAAPEVILQKAATVKSDIYSFSMIMQEILTDDIPWKGLDGSVVKKAVVSGNYLEADVRLPKPYYDIVKSGIHVKQKDRTMNLQDIRYILKNDLKDFTGAQRTQPTESPRVQRYGLHPDVNVYLGLTSEHPRETPDMEIIELKEMGSQPHSPRVHSLFTEGTLDPQAPDPCLMARETQNQDAPCPAPFMAEEASSPSTGQPSLCSFEINEIYSGCLILEDDIEEPPGAASSLEADGPNQVDELKSMEEELDKMEREACCFGSEDESSSKAETEYSFDDWDWQNGSLSSLSLPESTREAKSNLNNMSTTEEYLISKCVLDLKIMQTIMHENDDRLRNIEQILDEVEMKQKEQEERMSLWATSREFTNAYKLPLAVGPPSLNYIPPVLQLSGGQKPDTSGNYPTLPRFPRMLPTLCDPGKQNTDEQFQCTQGAKDSLETSRIQNTSSQGRPRESTAQAKATQFNSALFTLSSHRQGPSASPSCHWDSTRMSVEPVSSEIYNAESRNKDDGKVHLKWKMEVKEMAKKAATGQLTVPPWHPQSSLTLESEAENEPDALLQPPIRSPENTDWQRVIEYHRENDEPRGNGKFDKTGNNDCDSDQHGRQPRLGSFTSIRHPSPRQKEQPEHSEAFQASSDTLVAVEKSYSHQSMQSTCSPESSEDITDEFLTPDGEYFYSSTAQENLALETSSPIEEDFEGIQGAFAQPQVSGEEKFQMRKILGKNAEILPRSQFQPVRSTEDEQEETSKESPKELKEKDISLTDIQDLSSISYEPDSSFKEASCKTPKINHAPTSVSTPLSPGSVSSAASQYKDCLESITFQVKTEFASCWNSQEFIQTLSDDFISVRERAKKLDSLLTSSETPPSRLTGLKRLSSFIGAGSPSLVKACDSSPPHATQRRSLPKVEAFSQHHIDELPPPSQELLDDIELLKQQQGSSTVLHENTASDGGGTANDQRHLEEQETDSKKEDSSMLLSKETEDLGEDTERAHSTLDEDLERWLQPPEESVELQDLPKGSERETNIKDQKVGEEKRKREDSITPERRKSEGVLGTSEEDELKSCFWKRLGWSESSRIIVLDQSDLSD D7DC446A1A18FC4D rgap1_mouse Rac GTPase-activating protein 1 Mgcracgap Male germ cell RacGap Racgap1 protein protein mouse Mus musculus Mouse MDTTMVNLWTLFEQLVRRMEIINEGNESIEFIQVVKDFEDFRKKYQRTNQELEKFKDLLLKAETGRSALDVKLKHARNQVDVEIKRRQRAEAECAKLEQQIQLIRDILMCDTSGSIQLSEEQKSALAFLNRGQASSGHAGNNRLSTIDESGSILSDISFDKTDESLDWDSSLVKNFKMKKREKRRSNSRQFIDGPPGPVKKTCSIGSTVDQANESIVAKTTVTVPSDGGPIEAVSTIETLPSWTRSRGKSGPLQPVNSDSALNSRPLEPRTDTDNLGTPQNTGGMRLHDFVSKTVIKPESCVPCGKRIKFGKLSLKCRDCRLVSHPECRDRCPLPCIPPLVGTPVKIGEGMLADFVSQASPMIPAIVVSCVNEIEQRGLTEAGLYRISGCDRTVKELKEKFLKVKTVPLLSKVDDIHVICSLLKDFLRNLKEPLLTFWLSKAFMEAAEITDEDNSTAAMYQAVSELPQANRDTLAFLMIHLQRVSQSPDTKMDIANLAKVFGPTIVAHTVPNPDPVTMFQDIKRQLKVVERLLSLPLEYWNQFMMVDQENIDSQRGNGNSTPRTPDVKVSLLGPVTTPEFQLVKTPLSSSLSQRLYNLSKSTPRFGNKSKSATNLGQQGKFFPAPYLK DIP protein Q9WVM1 original sequence version: 63 8D1B9DEC3CE057BE tex14-cep55 7 9 unspecified role unspecified role prey prey n-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 10 unspecified role unspecified role bait bait n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 4a cep55-tex14 7 9 unspecified role unspecified role bait bait n-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 10 unspecified role unspecified role prey prey n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 4a tex14-tex14 3 10 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey physical association physical association Fig. 4b, Fig. 4c, Table 1 tex14-cep55-1 3 9 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait physical association physical association Fig. 4b, Table 1 cep55-tex14-1 3 9 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 37 39 physical association physical association Fig. 4c, Fig. 5b, Fig. 5c, Table 1 cep55-tex14-2 3 17 unspecified role unspecified role prey prey 16 unspecified role unspecified role bait bait physical association physical association Fig. 4c, Table 1 cep55-pdcd6ip 4 ALIX 14 unspecified role unspecified role prey prey c-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 16 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 45 47 physical association physical association Fig. 7c, Table 2 This interaction is suppressed by overexpression of the Tex14 fragment that interacts with Cep55. cep55-tsg101 4 13 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 16 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 50 52 physical association physical association Fig. 7c, Table 2 cep55-tex14-3 4 17 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 16 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 55 57 physical association physical association Fig. 7c, Fig. 7d, Table 2 tex14-cep55-2 4 17 unspecified role unspecified role bait bait gly795-pro796-pro797-tyr801ala required to bind necessary binding region required to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 16 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 61 63 physical association physical association Fig. 7e, Table 2 cep55-pdcd6ip-1 4 9 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ALIX 15 unspecified role unspecified role prey prey c-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 68 66 physical association physical association Fig. 8a, Fig. 9, Table 2 This interaction is suppressed by overexpression of the Tex14 fragment that interacts with Cep55. cep55-tsg101-1 4 9 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 12 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 71 73 physical association physical association Fig. 8b, Fig. 9, Table 2 This interaction is suppressed by overexpression of the Tex14 fragment that interacts with Cep55. cep55-tex14-4 4 10 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Q7M6U3:p.Pro793Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain P A Wed May 02 16:44:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro793ala' Tue Jun 30 17:24:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro793Ala' Q7M6U3:p.Pro792Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain P A Wed May 02 18:19:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro792ala' Tue Jun 30 19:33:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro792Ala' gly791-pro792-pro793-tyr797ala required to bind necessary binding region required to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Q7M6U3:p.Tyr797Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y A Wed May 02 15:38:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr797ala' Tue Jun 30 19:48:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr797Ala' 9 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 82 76 physical association physical association Fig. 7f, Fig. 8c, Fig. 9, Table 2 tex14-cep55-3 4 10 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain gly791ala-pro792ala-pro793ala-tyr797ala required to bind necessary binding region required to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 85 88 physical association physical association Table 2 tex14-kif23 3 MKLP1 11 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait physical association physical association Fig. 4b, Table 1 cep55-tex14-5 1 9 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 2, Fig. 10 cep55-tex14-6 5 10 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 1b cep55-kif23 8 10 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component MgcRacGap 18 unspecified role unspecified role neutral component neutral component MKLP1 11 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 1c cep55-tex14-7 2 10 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 1a cep55-tex14-8 6 10 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 3