DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu NANOS2 interacts with the CCR4-NOT deadenylation complex and leads to suppression of specific RNAs. NANOS2 interacts with the CCR4-NOT deadenylation complex and leads to suppression of specific RNAs. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2010 imex curation Suzuki A., Igarashi K., Aisaki K., Kanno J., Saga Y. ysaga@lab.nig.ac.jp 27-Mar-2013: Contacted by IntAct-Help. Only protein-protein interactions mouse-embryonic_testis Mus musculus embryonic_testis Embryonic male gonads (testis) anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Suzuki A., Igarashi K., Aisaki K., Kanno J., Saga Y. ysaga@lab.nig.ac.jp Accepted 2013-FEB-27 by PPORRAS 27-Mar-2013: Contacted by IntAct-Help. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2010 imex curation NANOS2 interacts with the CCR4-NOT deadenylation complex and leads to suppression of specific RNAs. NANOS2 interacts with the CCR4-NOT deadenylation complex and leads to suppression of specific RNAs. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2010 imex curation Suzuki A., Igarashi K., Aisaki K., Kanno J., Saga Y. ysaga@lab.nig.ac.jp 27-Mar-2013: Contacted by IntAct-Help. Only protein-protein interactions mouse-embryo Mus musculus embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti bait coip anti bait coimmunoprecipitation anti bait coip ms participant Identification by mass spectrometry ms participant Suzuki A., Igarashi K., Aisaki K., Kanno J., Saga Y. ysaga@lab.nig.ac.jp Accepted 2013-FEB-27 by PPORRAS 27-Mar-2013: Contacted by IntAct-Help. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2010 imex curation NANOS2 interacts with the CCR4-NOT deadenylation complex and leads to suppression of specific RNAs. NANOS2 interacts with the CCR4-NOT deadenylation complex and leads to suppression of specific RNAs. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2010 imex curation Suzuki A., Igarashi K., Aisaki K., Kanno J., Saga Y. ysaga@lab.nig.ac.jp 27-Mar-2013: Contacted by IntAct-Help. Only protein-protein interactions mouse-embryonic_testis Mus musculus embryonic_testis Embryonic male gonads (testis) imaging technique imaging technique microscopy immunostaining immunostaining Suzuki A., Igarashi K., Aisaki K., Kanno J., Saga Y. ysaga@lab.nig.ac.jp Accepted 2013-FEB-27 by PPORRAS 27-Mar-2013: Contacted by IntAct-Help. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2010 imex curation cno6l_mouse CCR4-NOT transcription complex subunit 6-like Cnot6l protein protein mouse Mus musculus Mouse MRLIGMPKEKYDPPDPRRIYTIMSAEEVANGKKSHWAELEISGRVRSLSTSLWSLTHLTALHLNDNNLARIPPDIAKLHNLVYLDLSSNKLRSLPAELGNMVSLRELLLNDNYLRVLPYELGRLFQLQTLGLTGNPLSQDIMSLYQDPDGTRKLLNFMLDNLAVHPEQLPPRPWITLKERDQILPSASFTVMCYNVLCDKYATRQLYGYCPSWALNWEYRKKGIMEEIVNWDADIISLQEVETEQYFTLFLPALKDRGYDGFFSPKSRAKIMSEQERKHVDGCAIFFKTEKFTLVQKHTVEFNQVAMANSDGSEAMLNRVMTKDNIGVAVVLEVHKELFGTGMKPIHAADKQLLIVANAHMHWDPEYSDVKLIQTMMFVSEVKNILEKASSRPGSPTADPNSIPLVLCADLNSLPDSGVVEYLSNGGVADNHKDFKELRYNECLMNFSCSGKNGSSEGRITHGFQLKSAYENNLMPYTNYTFDFKGVIDYIFYSKTHMNVLGVLGPLDPQWLVENNITGCPHPHIPSDHFSLLTQLELHPPLLPLVNGVHLPNRR FDC180DB315919DB cnot3_mouse CCR4-NOT transcription complex subunit 3 Cnot3 Not3 CCR4-associated factor 3 protein protein mouse Mus musculus Mouse MADKRKLQGEIDRCLKKVSEGVEQFEDIWQKLHNAANANQKEKYEADLKKEIKKLQRLRDQIKTWVASNEIKDKRQLIENRKLIETQMERFKVVERETKTKAYSKEGLGLAQKVDPAQKEKEEVGQWLTNTIDTLNMQVDQFESEVESLSVQTRKKKGDKDKQDRIEGLKRHIEKHRYHVRMLETILRMLDNDSILVDAIRKIKDDVEYYVDSSQDPDFEENEFLYDDLDLEDIPQALVATSPPSHSHMEDEIFNQSSSTPTSTTSSSPIPPSPANCTTENSEDDKKRGRSTDSEVSQSPAKNGSKPVHSNQHPQSPAVPPTYPSGPPPTTSALSSTPGNNGASTPAAPTSALGPKASPAPSHNSGTPAPYAQAVAPPNASGPSNAQPRPPSAQPSGGSGGGSGGSSSNSNSGTGGGAGKQNGATSYSSVVADSPAEVTLSSSGGSSASSQALGPTSGPHNPAPSTSKESSTAAPSGAGNVASGSGNNSGGPSLLVPLPVNPPSSPTPSFSEAKAAGTLLNGPPQFSTTPEIKAPEPLSSLKSMAERAAISSGIEDPVPTLHLTDRDIILSSTSAPPTSSQPPLQLSEVNIPLSLGVCPLGPVSLTKEQLYQQAMEEAAWHHMPHPSDSERIRQYLPRNPCPTPPYHHQMPPPHSDTVEFYQRLSTETLFFIFYYLEGTKAQYLAAKALKKQSWRFHTKYMMWFQRHEEPKTITDEFEQGTYIYFDYEKWGQRKKEGFTFEYRYLEDRDLQ 47C2A7321EDAB13A cnot9_mouse CCR4-NOT transcription complex subunit 9 Cnot9 Cell differentiation protein RQCD1 homolog EPO-induced protein FL10 Rcd1 Rqcd1 protein protein mouse Mus musculus Mouse MHSLATAAPVPTALAQVDREKIYQWINELSSPETRENALLELSKKRESVPDLAPMLWHSFGTIAALLQEIVNIYPSINPPTLTAHQSNRVCNALALLQCVASHPETRSAFLAAHIPLFLYPFLHTVSKTRPFEYLRLTSLGVIGALVKTDEQEVINFLLTTEIIPLCLRIMESGSELSKTVATFILQKILLDDTGLAYICQTYERFSHVAMILGKMVLQLSKEPSARLLKHVVRCYLRLSDNPRAREALRQCLPDQLKDTTFAQVLKDDTTTKRWLAQLVKNLQEGQVTDPRGIPLPPQ A4CA5DBE2E88DDA3 dcp1a_mouse mRNA-decapping enzyme 1A Dcp1a Mitc1 Smif MAD homolog 4-interacting transcription coactivator 1 Smad4-interacting transcriptional co-activator Transcription factor SMIF protein protein mouse Mus musculus Mouse MALSCSTVRPRRRGSALRSKMELLSRAEQEMSLAALKQHDPYITSIADLTGQVALYTFCPKANQWEKTDIEGTLFVYRRSASPYHGFTIVNRLNMHNLVEPVNKDLEFQLHEPFLLYRNASLSIYSIWFYDKNDCHRIAKLMADVVEEETRRSQQAARDKQSPSQANGCSDQRPIDILEMLSRAKDEYERNQMGGSNISSPGLQPSTQLSNLGSTETLEETPSGSQDKSAPSGHKHLTVEELFGTSLPKEQPTAMGLESEDTDKLLGDASQKEPSSFLPFPFEQSGGAPQSENLGIHSAAHHTVQPEVSTPVLITPASIAQSGDKHPPSYTLPLSPVLSPTLPAEAPTTQVPHLPRNSTMIQAVKTTPRQKSPLLNQPVPELSHSSLVASQSPFRAPVSLANPAGTALPSVDLLQKLRLTPQHDQIQAQPLGKGTMAPSFSSAAGQLATPESFIEPSSKTAAARAAVSASLSNMVLAPTLQSMQQNQDPEVFSQPKVLPSAIPIAGSPLVPATTTAVSSVLLSPSVFQQTVPRAADLERKASSPSPLTVGTAESQRKPSIILSKSQLQDTLIHLIKNDSSFLSTLHAVYLQVLTKNKDNHNL 55BC9C32BCA86CFD xrn1_mouse 5'-3' exoribonuclease 1 Xrn1 Dhm2 Exo Sep1 Protein Dhm2 Strand-exchange protein 1 homolog protein protein mouse Mus musculus Mouse MGVPKFYRWISERYPCLSEVVKEHQIPEFDNLYLDMNGIIHQCSHPNDDDVHFRISDDKIFTDIFHYLEVLFRIIKPRKVFFMAVDGVAPRAKMNQQRGRRFRSAKEAEDKIKKAIEKGETLPTEARFDSNCITPGTEFMARLHEHLKYFVNMKISTDKSWQGVTIYFSGHETPGEGEHKIMEFIRSEKAKPDHDPNTRHCLYGLDADLIMLGLTSHEAHFSLLREEVRFGGKKTQRVCAPEETTFHLLHLSLMREYIDYEFSALKEKITFKYDIEKIIDDWILMGFLVGNDFIPHLPHLHINHDALPLLYGTYIAILPELGGYINESGHLNLPRFERYLVKLSDFDREHFSEVFVDLKWFESKVGNKYLNEAAGAAAEEAKNCKEKRKPKGQENSLSWAALDKSEGEGVASRDNFEDETEDDDLFETEFRQYKRTYYMTKMGVDVVSDEFLANQAACYVQAIQWILHYYYHGVQSWSWYYPYHYAPFLSDIRSISTLKIHFELGKPFKPFEQLLAVLPSASKNLLPTCYQHLMTSEDSPIIEYYPPDFKTDLNGKQQEWEAVVLIPFIDETRLLEAMETCNHSLKKEERKRNQHSECLMCWYDRDTEFTYSSPWPEKFPAIERCCTRYKMISLDAWRVDINKNKITRVDQKALYFCGFPTLKHIKHKFFLKKSGVQVFQQSSRGENLMLEISVNAEPDELRIENIASAVLGKAVFVNWPHLEEARVVAVSDGETKFYIEEPPGTQKVYLGKTAPPSKVIQLTDKEQSNWTKEIQGISEQYLRRKGIIINETSAVVYAQLLTGRKYQISQNGEVRLEKQWSKQILPFVYQTIVKDIRAFDSRFSNIKTLDDLFPPRTMVFMLGTPYYGCTGEVQDSGDLITEGRIRVVFSIPCEPNLDALIQNQHKYSIKYNPGYVLAGRLGVSGYLVSRFTGSIFIGRGSRRNPHGDHKANVGLNLKFNKKNEEVPGYTKKVGNEWMYSSAAEQLLAEYIERAPELFSYIAKNSQEDVFYEDDIWPGENENGAEKVQEIITWLKGHPVSTLSRSSCDLHILDAAIVEKIEEEVEKCKQRKSNKKVRVTVKPHLMYRPLEQQHGVIPDRDAEFRLFDRVVNVRESFSVPVGLRGTVIGIKGASREADVLFEVLFDEEFPGGLTIRCSPGRGYRLPTSALVNLSHGSRCETGNQKLTAIVKPQPSVSHCSAAPSGHLGGLNHSPQSPFLPTQVPTKGDDEFCNIWQSLQGAGKTQHLQPTVQEKGAVLPQEISQVTEGHKSGFTDHSVRHQQRKHDSQRKFKEEYKSPKAECQSQKLSSKQTSGGSARCSIKLLKRNESPGTSEAQKVVTSYPNAVHKPPSGIENFLASLNLSKENEAQLPHHGEPPDEADLSPQSFAMKGTRMLKEILKIDSPDTRDSKNDMKKSDNEATVSSRRDERGVSAHPKPTCHMNKPHGTNEFQNVASVDSVCWPGQMPPVSTPVTELSRICSLVGMPQPDFSFLRTTQTMTVCQVKLSNGLLVHGPQCHSESEAKERAALFALQQLGSLGVSFPLPPPIFTNYPPAVPPGAVPPVFTQPTANIMPSSSHLFGSVSWRPPVPVAGNAFHYPSYPGTMPLAGGVPGGVHSQFIPLQVTKKRVANRKNFENKEAQSSQATPLQTNKPGSSEATKMTPQESPPASSSSSQAAQPVSSHVETASQGHVGSQPRSAPSSSKRKSRKLAVNFSVSKPSE 4FE6C888E3FE2459 cnot1_mouse CCR4-NOT transcription complex subunit 1 Cnot1 Kiaa1007 CCR4-associated factor 1 protein protein mouse Mus musculus Mouse MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECASLITKPNFISTLSYAIDNPLHYQKSLKPAPHLFAQLSKVLKLSKVQEVIFGLALLNSSSPDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGVAKTMMESSLADFMQEVGYGFCASIEECRNIIMQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSEGAQAHTWNVEVLIDVLKELNPSLNFKEVTYELDHPGFQIRDSKGLHNVVYGIQRGLGMEVFPVDFIYRPWKHAEGQLSFIQHSLINPEVFCFADYPCHTVATDILKAPPEDDNREIATWKSLDLIESLLRLAEVGQYEQVKQLFSFPIKHCPDMLVLALLQINTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGEQYDQAKLSRILDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRRCPSILGGLAPEKDQPKSAQLPAETLATMLACLQACAGSVSQELSETILTMVANCSNVMNKARQPPPGVMPKGRPPSASSLDAISPVQIDPLAGMASLSIGGSAAPHTQSMQGFPPNLGSAFSTPQSPAKAFPPLSTPNQTTAFSGIGGLSSQLPGGLGTGSLTGIGTGALGLPAVNNDPFVQRKLGTSGLNQPTFQQSKMKPSDLSQVWPEANQHFSKEIDDEANSYFQRIYNHPPHPTMSVDEVLEMLQRFKDSTIKREREVFNCMLRNLFEEYRFFPQYPDKELHITACLFGGIIEKGLVTYMALGLALRYVLEALRKPFGSKMYYFGIAALDRFKNRLKDYPQYCQHLASISHFMQFPHHLQEYIEYGQQSRDPPVKMQGSITTPGSIALAQAQAQAQVPAKAPLAGQVNTMVTTSTTTTVAKTVTVTKPTGVSFKKDVPPSINTTNIDTLLVATDQTERIVEPPENIQEKIAFIFNNLSQSNMTQKVEELKETVKEEFMPWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFNKMVLNETYRNIKVLLTSDKAAANFSDRSLLKNLGHWLGMITLAKNKPILHTDLDVKSLLLEAYVKGQQELLYVVPFVAKVLESSIRSLVFRPPNPWTMAIMNVLAELHQEHDLKLNLKFEIEVLCKNLALDINELKPGNLLKDKDRLKNLDEQLSAPKKDVKQPEELPAITTTTTSTTPATSTTCTATVPPQPQYSYHDINVYSLAGLAPHITLNPTIPLFQAHPQLKQCVRQAIERAVQELVHPVVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTAGMAMITCREPLLMSISTNLKNSFASALRTASPQQREMMDQAAAQLAQDNCELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPVVLTYQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPTNDLSQPTGFLAQPMKQAWATDDVAQIYDKCITELEQHLHAIPPTLAMNPQAQALRSLLEVVVLSRNSRDAIAALGLLQKAVEGLLDATSGADADLLLRYRECHLLVLKALQDGRAYGSPWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSMENGLNYMAVAFAMQLVKILLVDERSVAHITEADLFHTIETLMRINAHSRGNAPEGLPQLMEVVRSNYEAMIDRAHGGPNFMMHSGISQASEYDDPPGLREKAEYLLREWVNLYHSAAAGRDSTKAFSAFVGQMHQQGILKTDDLITRFFRLCTEMCVEISYRAQAEQQHNPAANPTMIRAKCYHNLDAFVRLIALLVKHSGEATNTVTKINLLNKVLGIVVGVLLQDHDVRQSEFQQLPYHRIFIMLLLELNAPEHVLETINFQTLTAFCNTFHILRPTKAPGFVYAWLELISHRIFIARMLAHTPQQKGWPMYAQLLIDLFKYLAPFLRNVELTKPMQILYKGTLRVLLVLLHDFPEFLCDYHYGFCDVIPPNCIQLRNLILSAFPRNMRLPDPFTPNLKVDMLSEINIAPRILTNFTGVMPPQFKKDLDSYLKTRSPVTFLSDLRSNLQVSNEPGNRYNLQLINALVLYVGTQAIAHIHNKGSTPSMSTITHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANTEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPAFKFWNHEFVHCAPEIEKLFQSVAQCCMGQKQAQQVMEGTGAS DIP protein Q6ZQ08 original sequence version: 42 DIP protein Q6ZQ08 original sequence version: 50 AfCS Y2H protein 39306. Translated from nucleotide sequence. 7771515027BA4B60 cnot7_mouse CCR4-NOT transcription complex subunit 7 CCR4-associated factor 1 Cnot7 Caf1 protein protein mouse Mus musculus Mouse MPAATVDHSQRICEVWACNLDEEMKKIRQVIRKYNYVAMDTEFPGVVARPIGEFRSNADYQYQLLRCNVDLLKIIQLGLTFMNEQGEYPPGTSTWQFNFKFNLTEDMYAQDSIELLTTSGIQFKKHEEEGIETQYFAELLMTSGVVLCEGVKWLSFHSGYDFGYLIKILTNSNLPEEELDFFEILRLFFPVIYDVKYLMKSCKNLKGGLQEVAEQLELERIGPQHQAGSDSLLTGMAFFKMREMFFEDHIDDAKYCGHLYGLGSGSSYVQNGTGNAYEEEASKQS DIP protein Q60809 original sequence version: 71 DIP protein Q60809 original sequence version: 78 DIP protein Q60809 original sequence version: 85 DIP protein Q60809 original sequence version: 126 2AF23EC38E06EFDB nano2_mouse Nanos homolog 2 Nanos2 Nos2 protein protein mouse Mus musculus Mouse MDLPPFDMWRDYFNLSQVVMDIIQSRKQRQEGEVAEEPNSRPQEKSEQDLEGYPGCLPTICNFCKHNGESRHVYTSHQLKTPEGVVVCPILRHYVCPLCGATGDQAHTLKYCPLNSSQQSLYRRSGRNSAGRRVKR 6D58AF6DD7DD63B1 nanos2-cnot1-1 2 9 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait physical association physical association 3A nanos2-cnot1-2 1 5 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association 3B cnot3-nanos2 3 5 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component colocalization colocalization 3E cnot3-dcp1a 3 5 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component colocalization colocalization 3H cnot3-cnot7 3 10 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component colocalization colocalization S6C cnot1-cnot7-1 3 11 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component colocalization colocalization S6I cnot1-dcp1a 3 7 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component colocalization colocalization S7C nanos2-xrn1 3 11 unspecified role unspecified role neutral component neutral component n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 8 unspecified role unspecified role neutral component neutral component colocalization colocalization S8F nanos2-cnot6l-1 1 4 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association 4C cnot3-dcp1a-1 3 11 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component colocalization colocalization S8C cnot1-nanos2 3 6 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component colocalization colocalization S6F