bhf-ucl Lipid phosphate phosphatase 3 stabilization of beta-catenin induces endothelial cell migration and formation of branching point structures. Lipid phosphate phosphatase 3 stabilization of beta-catenin induces endothelial cell migration and formation of branching point structures. Mol. Cell. Biol. (0270-7306) 2010 imex curation Humtsoe J.O., Liu M., Malik A.B., Wary K.K. Cardiac - Interactions involving cardiac related proteins kkwary@uic.edu 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. confocal microscopy confocal microscopy immunostaining immunostaining Humtsoe J.O., Liu M., Malik A.B., Wary K.K. Cardiac - Interactions involving cardiac related proteins kkwary@uic.edu Accepted 2013-NOV-07 by ORCHARD 22-Nov-2013: Contacted by IntAct-Help. Mol. Cell. Biol. (0270-7306) 2010 imex curation Lipid phosphate phosphatase 3 stabilization of beta-catenin induces endothelial cell migration and formation of branching point structures. Lipid phosphate phosphatase 3 stabilization of beta-catenin induces endothelial cell migration and formation of branching point structures. Mol. Cell. Biol. (0270-7306) 2010 imex curation Humtsoe J.O., Liu M., Malik A.B., Wary K.K. Cardiac - Interactions involving cardiac related proteins kkwary@uic.edu 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting western blot western blot Immuno blot Immuno blot Humtsoe J.O., Liu M., Malik A.B., Wary K.K. Cardiac - Interactions involving cardiac related proteins kkwary@uic.edu Accepted 2013-NOV-07 by ORCHARD 22-Nov-2013: Contacted by IntAct-Help. Mol. Cell. Biol. (0270-7306) 2010 imex curation Lipid phosphate phosphatase 3 stabilization of beta-catenin induces endothelial cell migration and formation of branching point structures. Lipid phosphate phosphatase 3 stabilization of beta-catenin induces endothelial cell migration and formation of branching point structures. Mol. Cell. Biol. (0270-7306) 2010 imex curation Humtsoe J.O., Liu M., Malik A.B., Wary K.K. Cardiac - Interactions involving cardiac related proteins kkwary@uic.edu 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Humtsoe J.O., Liu M., Malik A.B., Wary K.K. Cardiac - Interactions involving cardiac related proteins kkwary@uic.edu Accepted 2013-NOV-07 by ORCHARD 22-Nov-2013: Contacted by IntAct-Help. Mol. Cell. Biol. (0270-7306) 2010 imex curation Lipid phosphate phosphatase 3 stabilization of beta-catenin induces endothelial cell migration and formation of branching point structures. Lipid phosphate phosphatase 3 stabilization of beta-catenin induces endothelial cell migration and formation of branching point structures. Mol. Cell. Biol. (0270-7306) 2010 imex curation Humtsoe J.O., Liu M., Malik A.B., Wary K.K. Cardiac - Interactions involving cardiac related proteins kkwary@uic.edu 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions human-dermal_microvascular Human dermal microvascular endothelium cells (HDMVEC) dermal-microvascular_endothelium dermal microvascular endothelium anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Humtsoe J.O., Liu M., Malik A.B., Wary K.K. Cardiac - Interactions involving cardiac related proteins kkwary@uic.edu Accepted 2013-NOV-07 by ORCHARD 22-Nov-2013: Contacted by IntAct-Help. Mol. Cell. Biol. (0270-7306) 2010 imex curation Lipid phosphate phosphatase 3 stabilization of beta-catenin induces endothelial cell migration and formation of branching point structures. Lipid phosphate phosphatase 3 stabilization of beta-catenin induces endothelial cell migration and formation of branching point structures. Mol. Cell. Biol. (0270-7306) 2010 imex curation Humtsoe J.O., Liu M., Malik A.B., Wary K.K. Cardiac - Interactions involving cardiac related proteins kkwary@uic.edu 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions human-dermal_microvascular Human dermal microvascular endothelium cells (HDMVEC) dermal-microvascular_endothelium dermal microvascular endothelium confocal microscopy confocal microscopy immunostaining immunostaining Humtsoe J.O., Liu M., Malik A.B., Wary K.K. Cardiac - Interactions involving cardiac related proteins kkwary@uic.edu Accepted 2013-NOV-07 by ORCHARD 22-Nov-2013: Contacted by IntAct-Help. Mol. Cell. Biol. (0270-7306) 2010 imex curation Lipid phosphate phosphatase 3 stabilization of beta-catenin induces endothelial cell migration and formation of branching point structures. Lipid phosphate phosphatase 3 stabilization of beta-catenin induces endothelial cell migration and formation of branching point structures. Mol. Cell. Biol. (0270-7306) 2010 imex curation Humtsoe J.O., Liu M., Malik A.B., Wary K.K. Cardiac - Interactions involving cardiac related proteins kkwary@uic.edu 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Humtsoe J.O., Liu M., Malik A.B., Wary K.K. Cardiac - Interactions involving cardiac related proteins kkwary@uic.edu Accepted 2013-NOV-07 by ORCHARD 22-Nov-2013: Contacted by IntAct-Help. Mol. Cell. Biol. (0270-7306) 2010 imex curation Lipid phosphate phosphatase 3 stabilization of beta-catenin induces endothelial cell migration and formation of branching point structures. Lipid phosphate phosphatase 3 stabilization of beta-catenin induces endothelial cell migration and formation of branching point structures. Mol. Cell. Biol. (0270-7306) 2010 imex curation Humtsoe J.O., Liu M., Malik A.B., Wary K.K. Cardiac - Interactions involving cardiac related proteins kkwary@uic.edu 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions human-dermal_microvascular Human dermal microvascular endothelium cells (HDMVEC) dermal-microvascular_endothelium dermal microvascular endothelium anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Humtsoe J.O., Liu M., Malik A.B., Wary K.K. Cardiac - Interactions involving cardiac related proteins kkwary@uic.edu Accepted 2013-NOV-07 by ORCHARD 22-Nov-2013: Contacted by IntAct-Help. Mol. Cell. Biol. (0270-7306) 2010 imex curation lef1_human Lymphoid enhancer-binding factor 1 LEF1 T cell-specific transcription factor 1-alpha protein protein human Homo sapiens Human MPQLSGGGGGGGGDPELCATDEMIPFKDEGDPQKEKIFAEISHPEEEGDLADIKSSLVNESEIIPASNGHEVARQAQTSQEPYHDKAREHPDDGKHPDGGLYNKGPSYSSYSGYIMMPNMNNDPYMSNGSLSPPIPRTSNKVPVVQPSHAVHPLTPLITYSDEHFSPGSHPSHIPSDVNSKQGMSRHPPAPDIPTFYPLSPGGVGQITPPLGWQGQPVYPITGGFRQPYPSSLSVDTSMSRFSHHMIPGPPGPHTTGIPHPAIVTPQVKQEHPHTDSDLMHVKPQHEQRKEQEPKRPHIKKPLNAFMLYMKEMRANVVAECTLKESAAINQILGRRWHALSREEQAKYYELARKERQLHMQLYPGWSARDNYGKKKKRKREKLQESASGTGPRMTAAYI DIP protein Q9UJU2 original sequence version: 136 DIP protein Q9UJU2 original sequence version: 73 D480D440698EEFE3 ctnb1_human Catenin beta-1 Beta-catenin OK/SW-cl.35 PRO2286 CTNNB1 CTNNB protein protein human Homo sapiens Human MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLDEGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDEDQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLSHHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQKMVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTTSRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGMEGLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDREDITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNLALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGALHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEGATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETADLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGLPDLGHAQDLMDGLPPGDSNQLAWFDTDL DIP protein P35222 original sequence version: 187 DIP protein P35222 original sequence version: 188 DIP protein P35222 original sequence version: 180 DIP protein P35222-1 original sequence version: 181 DIP protein P35222 original sequence version: 181 DIP protein P35222 original sequence version: 107 DIP protein P35222-1 original sequence version: 107 DIP protein P35222 original sequence version: 103 DIP protein P35222-1 original sequence version: 103 DIP protein P35222 original sequence version: 175 DIP protein P35222 original sequence version: 136 DIP protein P35222 original sequence version: 135 DIP protein P35222 original sequence version: 131 DIP protein P35222-1 original sequence version: 133 DIP protein P35222 original sequence version: 133 DIP protein P35222 original sequence version: 148 DIP protein P35222-1 original sequence version: 139 DIP protein P35222 original sequence version: 139 DIP protein P35222 original sequence version: 146 DIP protein P35222 original sequence version: 196 DIP protein P35222 original sequence version: 192 DIP protein P35222 original sequence version: 185 DIP protein P35222 original sequence version: 151 DIP protein P35222-1 original sequence version: 151 DIP protein P35222-1 original sequence version: 171 DIP protein P35222-1 original sequence version: 148 DIP protein P35222 original sequence version: 177 DIP protein P35222 original sequence version: 178 DIP protein P35222-1 original sequence version: 178 CB78F165A3EEF86E cadh5_human Cadherin-5 CDH5 Vascular endothelial cadherin 7B4 antigen protein protein human Homo sapiens Human MQRLMMLLATSGACLGLLAVAAVAAAGANPAQRDTHSLLPTHRRQKRDWIWNQMHIDEEKNTSLPHHVGKIKSSVSRKNAKYLLKGEYVGKVFRVDAETGDVFAIERLDRENISEYHLTAVIVDKDTGENLETPSSFTIKVHDVNDNWPVFTHRLFNASVPESSAVGTSVISVTAVDADDPTVGDHASVMYQILKGKEYFAIDNSGRIITITKSLDREKQARYEIVVEARDAQGLRGDSGTATVLVTLQDINDNFPFFTQTKYTFVVPEDTRVGTSVGSLFVEDPDEPQNRMTKYSILRGDYQDAFTIETNPAHNEGIIKPMKPLDYEYIQQYSFIVEATDPTIDLRYMSPPAGNRAQVIINITDVDEPPIFQQPFYHFQLKENQKKPLIGTVLAMDPDAARHSIGYSIRRTSDKGQFFRVTKKGDIYNEKELDREVYPWYNLTVEAKELDSTGTPTGKESIVQVHIEVLDENDNAPEFAKPYQPKVCENAVHGQLVLQISAIDKDITPRNVKFKFILNTENNFTLTDNHDNTANITVKYGQFDREHTKVHFLPVVISDNGMPSRTGTSTLTVAVCKCNEQGEFTFCEDMAAQVGVSIQAVVAILLCILTITVITLLIFLRRRLRKQARAHGKSVPEIHEQLVTYDEEGGGEMDTTSYDVSVLNSVRRGGAKPPRPALDARPSLYAQVQKPPRHAPGAHGGPGEMAAMIEVKKDEADHDGDGPPYDTLHIYGYEGSESIAESLSSLGTDSSDSDVDYDFLNDWGPRFKMLAELYGSDPREELLY DIP protein P33151 original sequence version: 97 6DBEBFC4DA6899D1 pten_human Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN Phosphatase and tensin homolog Mutated in multiple advanced cancers 1 PTEN MMAC1 TEP1 protein protein human Homo sapiens Human MTAIIKEIVSRNKRRYQEDGFDLDLTYIYPNIIAMGFPAERLEGVYRNNIDDVVRFLDSKHKNHYKIYNLCAERHYDTAKFNCRVAQYPFEDHNPPQLELIKPFCEDLDQWLSEDDNHVAAIHCKAGKGRTGVMICAYLLHRGKFLKAQEALDFYGEVRTRDKKGVTIPSQRRYVYYYSYLLKNHLDYRPVALLFHKMMFETIPMFSGGTCNPQFVVCQLKVKIYSSNSGPTRREDKFMYFEFPQPLPVCGDIKVEFFHKQNKMLKKDKMFHFWVNTFFIPGPEETSEKVENGSLCDQEIDSICSIERADNDKEYLVLTLTKNDLDKANKDKANRYFSPNFKVKLYFTKTVEEPSNPEASSSTSVTPDVSDNEPDHYRYSDTTDSDPENEPFDEDQHTQITKV 75F97C3DD6802BA9 plpp3_human Phospholipid phosphatase 3 PLPP3 LPP3 Lipid phosphate phosphohydrolase 3 PAP2-beta PPAP2B Phosphatidate phosphohydrolase type 2b Phosphatidic acid phosphatase 2b Vascular endothelial growth factor and type I collagen-inducible protein protein protein human Homo sapiens Human MQNYKYDKAIVPESKNGGSPALNNNPRRSGSKRVLLICLDLFCLFMAGLPFLIIETSTIKPYHRGFYCNDESIKYPLKTGETINDAVLCAVGIVIAILAIITGEFYRIYYLKKSRSTIQNPYVAALYKQVGCFLFGCAISQSFTDIAKVSIGRLRPHFLSVCNPDFSQINCSEGYIQNYRCRGDDSKVQEARKSFFSGHASFSMYTMLYLVLYLQARFTWRGARLLRPLLQFTLIMMAFYTGLSRVSDHKHHPSDVLAGFAQGALVACCIVFFVSDLFKTKTTLSLPAPAIRKEILSPVDIIDRNNHHNMM CB3F60189044DA31 ctnd1_human Catenin delta-1 p120 catenin Cadherin-associated Src substrate p120(cas) CTNND1 KIAA0384 protein protein human Homo sapiens Human MDDSEVESTASILASVKEQEAQFEKLTRALEEERRHVSAQLERVRVSPQDANPLMANGTLTRRHQNGRFVGDADLERQKFSDLKLNGPQDHSHLLYSTIPRMQEPGQIVETYTEEDPEGAMSVVSVETSDDGTTRRTETTVKKVVKTVTTRTVQPVAMGPDGLPVDASSVSNNYIQTLGRDFRKNGNGGPGPYVGQAGTATLPRNFHYPPDGYSRHYEDGYPGGSDNYGSLSRVTRIEERYRPSMEGYRAPSRQDVYGPQPQVRVGGSSVDLHRFHPEPYGLEDDQRSMGYDDLDYGMMSDYGTARRTGTPSDPRRRLRSYEDMIGEEVPSDQYYWAPLAQHERGSLASLDSLRKGGPPPPNWRQPELPEVIAMLGFRLDAVKSNAAAYLQHLCYRNDKVKTDVRKLKGIPVLVGLLDHPKKEVHLGACGALKNISFGRDQDNKIAIKNCDGVPALVRLLRKARDMDLTEVITGTLWNLSSHDSIKMEIVDHALHALTDEVIIPHSGWEREPNEDCKPRHIEWESVLTNTAGCLRNVSSERSEARRKLRECDGLVDALIFIVQAEIGQKDSDSKLVENCVCLLRNLSYQVHREIPQAERYQEAAPNVANNTGPHAASCFGAKKGKDEWFSRGKKPIEDPANDTVDFPKRTSPARGYELLFQPEVVRIYISLLKESKTPAILEASAGAIQNLCAGRWTYGRYIRSALRQEKALSAIADLLTNEHERVVKAASGALRNLAVDARNKELIGKHAIPNLVKNLPGGQQNSSWNFSEDTVISILNTINEVIAENLEAAKKLRETQGIEKLVLINKSGNRSEKEVRAAALVLQTIWGYKELRKPLEKEGWKKSDFQVNLNNASRSQSSHSYDDSTLPLIDRNQKSDKKPDREEIQMSNMGSNTKSLDNNYSTPNERGDHNRTLDRSGDLGDMEPLKGTTPLMQDEGQESLEEELDVLVLDDEGGQVSYPSMQKI DIP protein O60716 original sequence version: 140 D5C37489A891F292 ctnnb1-lef-1 7 8 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association Fig. 1D, E. mouse LPP3: Q99JY8 human LPP3: O14495 ppap2b-ctnnd1-6 7 13 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait physical association physical association Fig. 7A, B, E, F, G The interaction was observed only in confluent cells. ctnnd1-ppap2b 7 13 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey physical association physical association Fig. 7D, E, F, H The interaction was observed only in confluent cells cdh5-ctnnb1-5 7 10 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association Fig. 7E, F ctnnd1-ppap2b-1 5 13 immunostaining immunostaining immunostaining immunostaining unspecified role unspecified role neutral component neutral component fixed cell fixed cell 12 immunostaining immunostaining immunostaining immunostaining unspecified role unspecified role neutral component neutral component fixed cell fixed cell colocalization colocalization Fig. 7J The colocalization was observed only in confluent cells. The colocalization was observed at the plasma membrane. ppap2b-ctnnd1-2 6 13 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 8B ctnnd1-ppap2b-3 1 12 immunostaining immunostaining anti-tag immunocytochemistry anti-tag immunocytochemistry immunostaining immunostaining unspecified role unspecified role neutral component neutral component fixed cell fixed cell region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13 immunostaining immunostaining immunostaining immunostaining unspecified role unspecified role neutral component neutral component fixed cell fixed cell colocalization colocalization Fig. 8H-J The cells in culture were confluent. The colocalization was observed at the plasma membrane. ppap2b-ctnnd1-3 2 12 unspecified role unspecified role bait bait c-cytoplasmic sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 13 unspecified role unspecified role prey prey human-dermal_microvascular Human dermal microvascular endothelium cells (HDMVEC) dermal-microvascular_endothelium dermal microvascular endothelium physical association physical association Fig. 9D ppap2b-ctnnd1-4 3 13 unspecified role unspecified role prey prey human-dermal_microvascular Human dermal microvascular endothelium cells (HDMVEC) dermal-microvascular_endothelium dermal microvascular endothelium 12 unspecified role unspecified role bait bait c-cytoplasmic sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) physical association physical association Fig. 9J ppap2b-ctnnd1-5 4 12 unspecified role unspecified role bait bait c-cytoplasmic required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 13 unspecified role unspecified role prey prey physical association physical association Fig. 10B pten-ctnnb1 7 9 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P60484:p.Cys124Ser mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain C S Wed May 02 15:02:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys124ser' Tue Jun 30 18:20:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys124Ser' physical association physical association Fig. 1G ppap2b-ctnnd1-7 7 12 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13 unspecified role unspecified role prey prey physical association physical association Fig. 8B