IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The Parkinson's disease kinase LRRK2 autophosphorylates its GTPase domain at multiple sites. The Parkinson's disease kinase LRRK2 autophosphorylates its GTPase domain at multiple sites. Biochem. Biophys. Res. Commun. (0006-291X) 2009 imex curation Greggio E., Taymans J.M., Zhen E.Y., Ryder J., Vancraenenbroeck R., Beilina A., Sun P., Deng J., Jaffe H., Baekelandt V., Merchant K., Cookson M.R. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease LRRK2 is assumed to be Human in origin when this is not explicitly stated, based on previous publications by the authors. Deng et al., 2008 states -human LRRK2 ROC domain (amino acids 1,333–1,516) was cloned into a modified pET28 b vector (pSKB3) with an N-terminal 6×His tag and a tobacco etch virus protease cleavage site. cookson@mail.nih.gov 01-March-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay sequence tag sequence tag identification sequence tag Greggio E., Taymans J.M., Zhen E.Y., Ryder J., Vancraenenbroeck R., Beilina A., Sun P., Deng J., Jaffe H., Baekelandt V., Merchant K., Cookson M.R. Parkinsons - Interactions investigated in the context of Parkinsons disease LRRK2 is assumed to be Human in origin when this is not explicitly stated, based on previous publications by the authors. Deng et al., 2008 states -human LRRK2 ROC domain (amino acids 1,333–1,516) was cloned into a modified pET28 b vector (pSKB3) with an N-terminal 6×His tag and a tobacco etch virus protease cleavage site. cookson@mail.nih.gov Accepted 2013-FEB-01 by ORCHARD 01-March-2013: Contacted by IntAct-Help. Biochem. Biophys. Res. Commun. (0006-291X) 2009 Only protein-protein interactions imex curation The Parkinson's disease kinase LRRK2 autophosphorylates its GTPase domain at multiple sites. The Parkinson's disease kinase LRRK2 autophosphorylates its GTPase domain at multiple sites. Biochem. Biophys. Res. Commun. (0006-291X) 2009 imex curation Greggio E., Taymans J.M., Zhen E.Y., Ryder J., Vancraenenbroeck R., Beilina A., Sun P., Deng J., Jaffe H., Baekelandt V., Merchant K., Cookson M.R. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease LRRK2 is assumed to be Human in origin when this is not explicitly stated, based on previous publications by the authors. Deng et al., 2008 states -human LRRK2 ROC domain (amino acids 1,333–1,516) was cloned into a modified pET28 b vector (pSKB3) with an N-terminal 6×His tag and a tobacco etch virus protease cleavage site. cookson@mail.nih.gov 01-March-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Greggio E., Taymans J.M., Zhen E.Y., Ryder J., Vancraenenbroeck R., Beilina A., Sun P., Deng J., Jaffe H., Baekelandt V., Merchant K., Cookson M.R. Parkinsons - Interactions investigated in the context of Parkinsons disease LRRK2 is assumed to be Human in origin when this is not explicitly stated, based on previous publications by the authors. Deng et al., 2008 states -human LRRK2 ROC domain (amino acids 1,333–1,516) was cloned into a modified pET28 b vector (pSKB3) with an N-terminal 6×His tag and a tobacco etch virus protease cleavage site. cookson@mail.nih.gov Accepted 2013-FEB-01 by ORCHARD 01-March-2013: Contacted by IntAct-Help. Biochem. Biophys. Res. Commun. (0006-291X) 2009 Only protein-protein interactions imex curation The Parkinson's disease kinase LRRK2 autophosphorylates its GTPase domain at multiple sites. The Parkinson's disease kinase LRRK2 autophosphorylates its GTPase domain at multiple sites. Biochem. Biophys. Res. Commun. (0006-291X) 2009 imex curation Greggio E., Taymans J.M., Zhen E.Y., Ryder J., Vancraenenbroeck R., Beilina A., Sun P., Deng J., Jaffe H., Baekelandt V., Merchant K., Cookson M.R. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease LRRK2 is assumed to be Human in origin when this is not explicitly stated, based on previous publications by the authors. Deng et al., 2008 states -human LRRK2 ROC domain (amino acids 1,333–1,516) was cloned into a modified pET28 b vector (pSKB3) with an N-terminal 6×His tag and a tobacco etch virus protease cleavage site. cookson@mail.nih.gov 01-March-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay sequence tag sequence tag identification sequence tag Greggio E., Taymans J.M., Zhen E.Y., Ryder J., Vancraenenbroeck R., Beilina A., Sun P., Deng J., Jaffe H., Baekelandt V., Merchant K., Cookson M.R. Parkinsons - Interactions investigated in the context of Parkinsons disease Selective reaction monitoring (SRM) was used to identify phosphorylation sites in LRRK2 (aas 1337–1347) by synthesizing all possible phosphorylated peptides from the sequences LMIVGNTGSGK and DYHFVNATEESDALAK and analysing to determine the peptide retention time and their fragmentation pattern. Phosphopeptides released from digestion of LRRK2 were then compared with these peptide standards. LRRK2 is assumed to be Human in origin when this is not explicitly stated, based on previous publications by the authors. Deng et al., 2008 states -human LRRK2 ROC domain (amino acids 1,333–1,516) was cloned into a modified pET28 b vector (pSKB3) with an N-terminal 6×His tag and a tobacco etch virus protease cleavage site. cookson@mail.nih.gov Accepted 2013-FEB-01 by ORCHARD 01-March-2013: Contacted by IntAct-Help. Biochem. Biophys. Res. Commun. (0006-291X) 2009 Only protein-protein interactions imex curation lrrk2_human Leucine-rich repeat serine/threonine-protein kinase 2 LRRK2 PARK8 Dardarin protein protein human Homo sapiens Human MASGSCQGCEEDEETLKKLIVRLNNVQEGKQIETLVQILEDLLVFTYSERASKLFQGKNIHVPLLIVLDSYMRVASVQQVGWSLLCKLIEVCPGTMQSLMGPQDVGNDWEVLGVHQLILKMLTVHNASVNLSVIGLKTLDLLLTSGKITLLILDEESDIFMLIFDAMHSFPANDEVQKLGCKALHVLFERVSEEQLTEFVENKDYMILLSALTNFKDEEEIVLHVLHCLHSLAIPCNNVEVLMSGNVRCYNIVVEAMKAFPMSERIQEVSCCLLHRLTLGNFFNILVLNEVHEFVVKAVQQYPENAALQISALSCLALLTETIFLNQDLEEKNENQENDDEGEEDKLFWLEACYKALTWHRKNKHVQEAACWALNNLLMYQNSLHEKIGDEDGHFPAHREVMLSMLMHSSSKEVFQASANALSTLLEQNVNFRKILLSKGIHLNVLELMQKHIHSPEVAESGCKMLNHLFEGSNTSLDIMAAVVPKILTVMKRHETSLPVQLEALRAILHFIVPGMPEESREDTEFHHKLNMVKKQCFKNDIHKLVLAALNRFIGNPGIQKCGLKVISSIVHFPDALEMLSLEGAMDSVLHTLQMYPDDQEIQCLGLSLIGYLITKKNVFIGTGHLLAKILVSSLYRFKDVAEIQTKGFQTILAILKLSASFSKLLVHHSFDLVIFHQMSSNIMEQKDQQFLNLCCKCFAKVAMDDYLKNVMLERACDQNNSIMVECLLLLGADANQAKEGSSLICQVCEKESSPKLVELLLNSGSREQDVRKALTISIGKGDSQIISLLLRRLALDVANNSICLGGFCIGKVEPSWLGPLFPDKTSNLRKQTNIASTLARMVIRYQMKSAVEEGTASGSDGNFSEDVLSKFDEWTFIPDSSMDSVFAQSDDLDSEGSEGSFLVKKKSNSISVGEFYRDAVLQRCSPNLQRHSNSLGPIFDHEDLLKRKRKILSSDDSLRSSKLQSHMRHSDSISSLASEREYITSLDLSANELRDIDALSQKCCISVHLEHLEKLELHQNALTSFPQQLCETLKSLTHLDLHSNKFTSFPSYLLKMSCIANLDVSRNDIGPSVVLDPTVKCPTLKQFNLSYNQLSFVPENLTDVVEKLEQLILEGNKISGICSPLRLKELKILNLSKNHISSLSENFLEACPKVESFSARMNFLAAMPFLPPSMTILKLSQNKFSCIPEAILNLPHLRSLDMSSNDIQYLPGPAHWKSLNLRELLFSHNQISILDLSEKAYLWSRVEKLHLSHNKLKEIPPEIGCLENLTSLDVSYNLELRSFPNEMGKLSKIWDLPLDELHLNFDFKHIGCKAKDIIRFLQQRLKKAVPYNRMKLMIVGNTGSGKTTLLQQLMKTKKSDLGMQSATVGIDVKDWPIQIRDKRKRDLVLNVWDFAGREEFYSTHPHFMTQRALYLAVYDLSKGQAEVDAMKPWLFNIKARASSSPVILVGTHLDVSDEKQRKACMSKITKELLNKRGFPAIRDYHFVNATEESDALAKLRKTIINESLNFKIRDQLVVGQLIPDCYVELEKIILSERKNVPIEFPVIDRKRLLQLVRENQLQLDENELPHAVHFLNESGVLLHFQDPALQLSDLYFVEPKWLCKIMAQILTVKVEGCPKHPKGIISRRDVEKFLSKKRKFPKNYMSQYFKLLEKFQIALPIGEEYLLVPSSLSDHRPVIELPHCENSEIIIRLYEMPYFPMGFWSRLINRLLEISPYMLSGRERALRPNRMYWRQGIYLNWSPEAYCLVGSEVLDNHPESFLKITVPSCRKGCILLGQVVDHIDSLMEEWFPGLLEIDICGEGETLLKKWALYSFNDGEEHQKILLDDLMKKAEEGDLLVNPDQPRLTIPISQIAPDLILADLPRNIMLNNDELEFEQAPEFLLGDGSFGSVYRAAYEGEEVAVKIFNKHTSLRLLRQELVVLCHLHHPSLISLLAAGIRPRMLVMELASKGSLDRLLQQDKASLTRTLQHRIALHVADGLRYLHSAMIIYRDLKPHNVLLFTLYPNAAIIAKIADYGIAQYCCRMGIKTSEGTPGFRAPEVARGNVIYNQQADVYSFGLLLYDILTTGGRIVEGLKFPNEFDELEIQGKLPDPVKEYGCAPWPMVEKLIKQCLKENPQERPTSAQVFDILNSAELVCLTRRILLPKNVIVECMVATHHNSRNASIWLGCGHTDRGQLSFLDLNTEGYTSEEVADSRILCLALVHLPVEKESWIVSGTQSGTLLVINTEDGKKRHTLEKMTDSVTCLYCNSFSKQSKQKNFLLVGTADGKLAIFEDKTVKLKGAAPLKILNIGNVSTPLMCLSESTNSTERNVMWGGCGTKIFSFSNDFTIQKLIETRTSQLFSYAAFSDSNIITVVVDTALYIAKQNSPVVEVWDKKTEKLCGLIDCVHFLREVMVKENKESKHKMSYSGRVKTLCLQKNTALWIGTGGGHILLLDLSTRRLIRVIYNFCNSVRVMMTAQLGSLKNVMLVLGYNRKNTEGTQKQKEIQSCLTVWDINLPHEVQNLEKHIEVRKELAEKMRRTSVE DIP protein Q5S007 original sequence version: 36 DIP protein Q5S007 original sequence version: 68 DIP protein Q5S007 original sequence version: 64 26142A0CECBBC3F4 lrrk2-lrrk2-44 2 4 enzyme enzyme neutral component neutral component n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 4 enzyme target enzyme target substrate substrate neutral component neutral component n_terminus his L-histidine residue (2S)-2-amino-3-(1H-imidazol-4-yl)propanoic acid (2S)-2-amino-3-(1H-imidazol-5-yl)propanoic acid [tautomer] 2-azanyl-3-(1H-imidazol-4-yl)propanoic acid 2-azanyl-3-(1H-imidazol-5-yl)propanoic acid [tautomer] 4-(2-amino-2-carboxyethyl)imidazole L-histidine alpha-amino-beta-(4-imidazole)propionic acid glyoxaline-5-alanine His n-term range n-terminal range n-term range n-term range n-terminal range n-term range roc_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 8 11 phosphorylation phosphorylation reaction phosphorylation 1A GST-LRRK2 was expressed by Baculovirus in Insect cells. LRRK (2970–2527) was able to phosphorylate the ROC fragment and the ROC-COR bidomain but not the kinase domain. lrrk2-lrrk2-45 2 4 enzyme target enzyme target substrate substrate neutral component neutral component roc_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 4 enzyme enzyme neutral component neutral component n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 14 18 phosphorylation phosphorylation reaction phosphorylation 1B GST-LRRK2 was expressed by Baculovirus in Insect cells. lrrk2-lrrk2-46 2 4 enzyme enzyme neutral component neutral component n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q5S007:p.Gly2019Ser mutation increasing rate mutation increasing interaction rate mutation increasing rate certain certain sequence position certain certain certain sequence position certain G S Wed May 02 14:34:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly2019ser' Tue Jun 30 19:01:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly2019Ser' Q5S007:p.Lys1906Met mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain K M Wed May 02 18:44:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys1906met' Tue Jun 30 18:12:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys1906Met' human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 4 enzyme target enzyme target substrate substrate neutral component neutral component n_terminus his L-histidine residue (2S)-2-amino-3-(1H-imidazol-4-yl)propanoic acid (2S)-2-amino-3-(1H-imidazol-5-yl)propanoic acid [tautomer] 2-azanyl-3-(1H-imidazol-4-yl)propanoic acid 2-azanyl-3-(1H-imidazol-5-yl)propanoic acid [tautomer] 4-(2-amino-2-carboxyethyl)imidazole L-histidine alpha-amino-beta-(4-imidazole)propionic acid glyoxaline-5-alanine His n-term range n-terminal range n-term range n-term range n-terminal range n-term range roc_domain sufficient to bind sufficient binding region sufficient to bind range range range range ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation 1C The text states "we used ~50 nM 3×flag-tagged wild-type, K1906M or G2019S LRRK2 from mammalian cells and ~2 µM of his-tagged ROC domain from E. coli.(Fig. 1C)" However it would appear that TWO his tagged ROC domains expressed in E.coli are used in this publication. (amino acids 1333–1516 and 1335–1548.) lrrk2-lrrk2-47 1 4 enzyme enzyme neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 4 enzyme target enzyme target substrate substrate neutral component neutral component roc_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Table1 GST-LRRK2 was expressed by Baculovirus in Insect cells. Seven potential phosphosites were found in either LRRK2 aas 970–2527 or isolated ROC. lrrk2-1 1 4 putative self putative self putative self putative self n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation true Table1ii GST-LRRK2 was expressed by Baculovirus in Insect cells. Seven potential phosphosites were found in either LRRK2 aas 970–2527 or isolated ROC. Two peptides were identified outside of the ROC domain using LRRK2 aas 970–2527. lrrk2-1 3 4 putative self putative self putative self putative self c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection phosphorylation phosphorylation reaction phosphorylation true Fig 2, Table 2. T1343 and T1491 were unambiguously identified as phosphosites. lrrk2-lrrk2-48 2 4 enzyme target enzyme target substrate substrate neutral component neutral component roc_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 4 enzyme enzyme neutral component neutral component Q5S007:p.Ser1403Ala mutation increasing rate mutation increasing interaction rate mutation increasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain S A Wed May 02 14:24:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser1403ala' Tue Jun 30 17:01:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser1403Ala' n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q5S007:p.Thr1348Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain T A Wed May 02 16:57:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr1348ala' Tue Jun 30 16:17:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr1348Ala' Q5S007:p.Thr1404Ala mutation increasing rate mutation increasing interaction rate mutation increasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain T A Wed May 02 17:09:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr1404ala' Tue Jun 30 16:09:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr1404Ala' Q5S007:p.Thr1343Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain T A Wed May 02 17:07:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr1343ala' Tue Jun 30 19:14:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr1343Ala' Q5S007:p.Thr1491Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain T A Wed May 02 16:21:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr1491ala' Tue Jun 30 15:35:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr1491Ala' Q5S007:p.Thr1410Ala mutation increasing rate mutation increasing interaction rate mutation increasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain T A Wed May 02 19:13:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr1410ala' Tue Jun 30 16:19:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr1410Ala' Q5S007:p.Thr1452Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain T A Wed May 02 16:24:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr1452ala' Tue Jun 30 19:57:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr1452Ala' Q5S007:p.Thr1349Ala mutation increasing rate mutation increasing interaction rate mutation increasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain T A Wed May 02 15:16:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr1349ala' Tue Jun 30 17:41:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr1349Ala' Q5S007:p.Thr1503Ala mutation increasing rate mutation increasing interaction rate mutation increasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain T A Wed May 02 17:50:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr1503ala' Tue Jun 30 19:29:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr1503Ala' human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. phosphorylation phosphorylation reaction phosphorylation 3A/B lrrk2-1 2 4 putative self putative self putative self putative self c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q5S007:p.Asp2017Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain D A Wed May 02 19:28:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp2017ala' Tue Jun 30 15:39:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp2017Ala' n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q5S007:p.Gly2019Ser mutation increasing rate mutation increasing interaction rate mutation increasing rate certain certain sequence position certain certain certain sequence position certain G S Wed May 02 17:14:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly2019ser' Tue Jun 30 20:09:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly2019Ser' spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection phosphorylation phosphorylation reaction phosphorylation true Fig S1 phosphorylation of T1343 and T1491