IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ DiaA dynamics are coupled with changes in initial origin complexes leading to helicase loading. DiaA dynamics are coupled with changes in initial origin complexes leading to helicase loading. J. Biol. Chem. (0021-9258) 2009 imex curation Keyamura K., Abe Y., Higashi M., Ueda T., Katayama T. katayama@phar.kyuhsu-u.ac.jp 27-Mar-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Keyamura K., Abe Y., Higashi M., Ueda T., Katayama T. katayama@phar.kyuhsu-u.ac.jp Accepted 2013-MAR-06 by ORCHARD 27-Mar-2013: Contacted by IntAct-Help. J. Biol. Chem. (0021-9258) 2009 Only protein-protein interactions imex curation DiaA dynamics are coupled with changes in initial origin complexes leading to helicase loading. DiaA dynamics are coupled with changes in initial origin complexes leading to helicase loading. J. Biol. Chem. (0021-9258) 2009 imex curation Keyamura K., Abe Y., Higashi M., Ueda T., Katayama T. katayama@phar.kyuhsu-u.ac.jp 27-Mar-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip weight by comassie molecular weight estimation by coomasie staining weight by comassie Keyamura K., Abe Y., Higashi M., Ueda T., Katayama T. katayama@phar.kyuhsu-u.ac.jp Accepted 2013-MAR-06 by ORCHARD 27-Mar-2013: Contacted by IntAct-Help. J. Biol. Chem. (0021-9258) 2009 Only protein-protein interactions imex curation DiaA dynamics are coupled with changes in initial origin complexes leading to helicase loading. DiaA dynamics are coupled with changes in initial origin complexes leading to helicase loading. J. Biol. Chem. (0021-9258) 2009 imex curation Keyamura K., Abe Y., Higashi M., Ueda T., Katayama T. katayama@phar.kyuhsu-u.ac.jp 27-Mar-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro filter trap assay filter trap assay Filter retention assay Filter retention assay predetermined predetermined participant predetermined Keyamura K., Abe Y., Higashi M., Ueda T., Katayama T. katayama@phar.kyuhsu-u.ac.jp Accepted 2013-MAR-06 by ORCHARD 27-Mar-2013: Contacted by IntAct-Help. J. Biol. Chem. (0021-9258) 2009 Only protein-protein interactions imex curation diaa_ecoli DnaA initiator-associating protein DiaA diaA b3149 JW3118 yraO protein protein ecoli Escherichia coli (strain K12) MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKILCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQVRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVEIRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD F9ADFE9C0DF365A8 atp ATP Adenosine 5'-triphosphate Adenosine triphosphate ADENOSINE-5'-TRIPHOSPHATE Adephos Adetol Adynol Atipi ATP Atriphos Cardenosine Fosfobion Glucobasin H4atp Myotriphos Triadenyl Triphosphaden adenosine 5'-(tetrahydrogen triphosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) dnaa_ecoli Chromosomal replication initiator protein DnaA dnaA b3702 JW3679 protein protein ecoli Escherichia coli (strain K12) MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPNRFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVAQTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADNPGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNAIEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEINGVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVRELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVADLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREESHDIKEDFSNLIRTLSS DIP protein P03004 original sequence version: 109 DIP protein P03004 original sequence version: null 607C8366A8CDCCED adp adenosine 5'-(trihydrogen diphosphate) (ADP) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) diaa-dnaa-1 1 6 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role neutral component neutral component P66817:p.Pro72Ala mutation mutation certain certain sequence position certain certain certain sequence position certain P A Wed May 02 16:34:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro72ala' Tue Jun 30 15:58:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro72Ala' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 1a-d diaa-dnaa-2 2 6 unspecified role unspecified role prey prey P03004:p.Phe46Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain F A Wed May 02 14:50:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe46ala' Tue Jun 30 19:48:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe46Ala' P03004:p.Glu21Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain E A Wed May 02 19:47:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu21ala' Tue Jun 30 18:24:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu21Ala' ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role bait bait n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 2a-b dnaa-atp 3 6 unspecified role unspecified role neutral component neutral component P03004:p.Phe46Ala mutation mutation certain certain sequence position certain certain certain sequence position certain F A Wed May 02 18:29:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe46ala' Tue Jun 30 19:56:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe46Ala' ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Fig. 2e dnaa-adp 3 7 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role neutral component neutral component P03004:p.Phe46Ala mutation mutation certain certain sequence position certain certain certain sequence position certain F A Wed May 02 20:11:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe46ala' Tue Jun 30 18:27:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe46Ala' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 2e