UniProt UniProt http://www.uniprot.org The non-canonical Hop protein from Caenorhabditis elegans exerts essential functions and forms binary complexes with either Hsc70 or Hsp90. The non-canonical Hop protein from Caenorhabditis elegans exerts essential functions and forms binary complexes with either Hsc70 or Hsp90. J. Mol. Biol. (0022-2836) 2009 imex curation Gaiser A.M., Brandt F., Richter K. klaus.richter@ch.tum.de 18-Feb-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro atpase assay atpase assay predetermined predetermined participant predetermined Gaiser A.M., Brandt F., Richter K. klaus.richter@ch.tum.de Accepted 2014-JAN-30 by ORCHARD J. Mol. Biol. (0022-2836) 2009 imex curation The non-canonical Hop protein from Caenorhabditis elegans exerts essential functions and forms binary complexes with either Hsc70 or Hsp90. The non-canonical Hop protein from Caenorhabditis elegans exerts essential functions and forms binary complexes with either Hsc70 or Hsp90. J. Mol. Biol. (0022-2836) 2009 imex curation Gaiser A.M., Brandt F., Richter K. klaus.richter@ch.tum.de 18-Feb-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Gaiser A.M., Brandt F., Richter K. klaus.richter@ch.tum.de Accepted 2014-JAN-30 by ORCHARD J. Mol. Biol. (0022-2836) 2009 imex curation The non-canonical Hop protein from Caenorhabditis elegans exerts essential functions and forms binary complexes with either Hsc70 or Hsp90. The non-canonical Hop protein from Caenorhabditis elegans exerts essential functions and forms binary complexes with either Hsc70 or Hsp90. J. Mol. Biol. (0022-2836) 2009 imex curation Gaiser A.M., Brandt F., Richter K. klaus.richter@ch.tum.de 18-Feb-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Gaiser A.M., Brandt F., Richter K. klaus.richter@ch.tum.de Accepted 2014-JAN-30 by ORCHARD J. Mol. Biol. (0022-2836) 2009 imex curation hsp90_caeel Heat shock protein 90 daf-21 Abnormal dauer formation protein 21 C47E8.5 protein protein caeel Caenorhabditis elegans MSENAETFAFQAEIAQLMSLIINTFYSNKEIYLRELISNASDALDKIRYQALTEPSELDTGKELFIKITPNKEEKTLTIMDTGIGMTKADLVNNLGTIAKSGTKAFMEALQAGADISMIGQFGVGFYSAFLVADKVVVTSKNNDDDSYQWESSAGGSFVVRPFNDPEVTRGTKIVMHIKEDQIDFLEERKIKEIVKKHSQFIGYPIKLVVEKEREKEVEDEEAVEAKDEEKKEGEVENVADDADKKKTKKIKEKYFEDEELNKTKPIWTRNPDDISNEEYAEFYKSLSNDWEDHLAVKHFSVEGQLEFRALLFVPQRAPFDLFENKKSKNSIKLYVRRVFIMENCEELMPEYLNFIKGVVDSEDLPLNISREMLQQSKILKVIRKNLVKKCMELIDEVAEDKDNFKKFYEQFGKNLKLGIHEDSTNRKKLSDFLRYSTSAGDEPTSLKEYVSRMKENQTQIYYITGESKDVVAASAFVERVKSRGFEVLYMCDPIDEYCVQQLKEYDGKKLVSVTKEGLELPETEEEKKKFEEDKVAYENLCKVIKDILEKKVEKVGVSNRLVSSPCCIVTSEYGWSANMERIMKAQALRDSSTMGYMAAKKHLEINPDHAIMKTLRDRVEVDKNDKTVKDLVVLLFETALLASGFSLEEPQSHASRIYRMIKLGLDIGDDEIEDSAVPSSCTAEAKIEGAEEDASRMEEVD 2B0E975A24074811 atp ATP Adenosine 5'-triphosphate Adenosine triphosphate ADENOSINE-5'-TRIPHOSPHATE Adephos Adetol Adynol Atipi ATP Atriphos Cardenosine Fosfobion Glucobasin H4atp Myotriphos Triadenyl Triphosphaden adenosine 5'-(tetrahydrogen triphosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) hsp1_caeel Heat shock protein hsp-1 hsp-1 hsp70a F26D10.3 Heat shock 70 kDa protein A protein protein caeel Caenorhabditis elegans MSKHNAVGIDLGTTYSCVGVFMHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPHNTVFDAKRLIGRKFDDPAVQSDMKHWPFKVISAEGAKPKVQVEYKGENKIFTPEEISSMVLLKMKETAEAFLGTTVKDAVVTVPAYFNDSQRQATKDAGAIAGLNVLRIINEPTAAAIAYGLDKKGHGERNVLIFDLGGGTFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVNHFCAEFKRKHKKDLASNPRALRRLRTACERAKRTLSSSSQASIEIDSLFEGIDFYTNITRARFEELCADLFRSTMDPVEKSLRDAKMDKSQVHDIVLVGGSTRIPKVQKLLSDLFSGKELNKSINPDEAVAYGAAVQAAILSGDKSEAVQDLLLLDVAPLSLGIETAGGVMTALIKRNTTIPTKTAQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRGVPQIEVTFDIDANGILNVSATDKSTGKQNKITITNDKGRLSKDDIERMVNEAEKYKADDEAQKDRIGAKNGLESYAFNLKQTIEDEKLKDKISPEDKKKIEDKCDEILKWLDSNQTAEKEEFEHQQKDLEGLANPIISKLYQSAGGAPPGAAPGGAAGGAGGPTIEEVD 5D7B46B812E7A1DA stip1_caeel Stress-induced-phosphoprotein 1 sti-1 Hsc70/Hsp90-organizing protein R09E12.3 protein protein caeel Caenorhabditis elegans MTDAAIAEKDLGNAAYKQKDFEKAHVHYDKAIELDPSNITFYNNKAAVYFEEKKFAECVQFCEKAVEVGRETRADYKLIAKAMSRAGNAFQKQNDLSLAVQWFHRSLSEFRDPELVKKVKELEKQLKAAERLAYINPELAQEEKNKGNEYFKKGDYPTAMRHYNEAVKRDPENAILYSNRAACLTKLMEFQRALDDCDTCIRLDSKFIKGYIRKAACLVAMREWSKAQRAYEDALQVDPSNEEAREGVRNCLRSNDEDPEKAKERSLADPEVQEILRDPGMRMILEQMSNDPGAVREHLKNPEIFQKLMKLRDAGVIQMR 9D21907275A09780 cehop-hap90 3 CeHop 7 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag anti tag western anti tag western blot anti tag western undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) hsp90 4 unspecified role unspecified role prey prey cell lysate cell lysate caeel Caenorhabditis elegans physical association physical association Fig 3a daf_21-sti_1 2 hsp90 4 unspecified role unspecified role bait bait cell lysate cell lysate region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) CeHop 7 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig 3b, 3C, Table 3 Both the N-terminal (1-131) and C-terminal (191-32) domains of sti-1 were capable of weakly binding sti_1 sti1-hsc70 2 7 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 4a, Table 3 Both the N-terminal (1-131) and C-terminal (191-32) domains of sti-1 were capable of weakly binding hsc-70 atp-hsp_1 1 5 enzyme target enzyme target substrate substrate unspecified role unspecified role ecoli Escherichia coli (strain K12) 6 enzyme enzyme unspecified role unspecified role ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 4B hsp_1-daf_21 2 6 unspecified role unspecified role bait bait CeHop 7 unspecified role unspecified role prey prey caeel Caenorhabditis elegans hsp90 4 competitor competitor competitor prey prey ecoli Escherichia coli (strain K12) physical association physical association Fig. 5 atp-daf_21 1 4 enzyme enzyme unspecified role unspecified role ecoli Escherichia coli (strain K12) 5 enzyme target enzyme target substrate substrate unspecified role unspecified role ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 3D atp-daf_21-1 1 4 enzyme enzyme unspecified role unspecified role ecoli Escherichia coli (strain K12) 5 enzyme target enzyme target substrate substrate unspecified role unspecified role ecoli Escherichia coli (strain K12) 7 inhibitor inhibitor unspecified role unspecified role ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 3D