UniProt UniProt http://www.uniprot.org SOD1 mutations disrupt redox-sensitive Rac regulation of NADPH oxidase in a familial ALS model. SOD1 mutations disrupt redox-sensitive Rac regulation of NADPH oxidase in a familial ALS model. J. Clin. Invest. (0021-9738) 2008 imex curation Harraz M.M., Marden J.J., Zhou W., Zhang Y., Williams A., Sharov V.S., Nelson K., Luo M., Paulson H., Schöneich C., Engelhardt J.F. john-engelhardt@uiowa.edu 17-Jun-2013: Contacted by IntAct-Help. Only protein-protein interactions mouse-brain Mus musculus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Harraz M.M., Marden J.J., Zhou W., Zhang Y., Williams A., Sharov V.S., Nelson K., Luo M., Paulson H., Schöneich C., Engelhardt J.F. john-engelhardt@uiowa.edu Accepted 2013-MAY-17 by ORCHARD 17-Jun-2013: Contacted by IntAct-Help. J. Clin. Invest. (0021-9738) 2008 Only protein-protein interactions imex curation SOD1 mutations disrupt redox-sensitive Rac regulation of NADPH oxidase in a familial ALS model. SOD1 mutations disrupt redox-sensitive Rac regulation of NADPH oxidase in a familial ALS model. J. Clin. Invest. (0021-9738) 2008 imex curation Harraz M.M., Marden J.J., Zhou W., Zhang Y., Williams A., Sharov V.S., Nelson K., Luo M., Paulson H., Schöneich C., Engelhardt J.F. john-engelhardt@uiowa.edu 17-Jun-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Harraz M.M., Marden J.J., Zhou W., Zhang Y., Williams A., Sharov V.S., Nelson K., Luo M., Paulson H., Schöneich C., Engelhardt J.F. john-engelhardt@uiowa.edu Accepted 2013-MAY-17 by ORCHARD 17-Jun-2013: Contacted by IntAct-Help. J. Clin. Invest. (0021-9738) 2008 Only protein-protein interactions imex curation SOD1 mutations disrupt redox-sensitive Rac regulation of NADPH oxidase in a familial ALS model. SOD1 mutations disrupt redox-sensitive Rac regulation of NADPH oxidase in a familial ALS model. J. Clin. Invest. (0021-9738) 2008 imex curation Harraz M.M., Marden J.J., Zhou W., Zhang Y., Williams A., Sharov V.S., Nelson K., Luo M., Paulson H., Schöneich C., Engelhardt J.F. john-engelhardt@uiowa.edu 17-Jun-2013: Contacted by IntAct-Help. Only protein-protein interactions mouse-kidney Mus musculus kidney kidney [Renal] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Harraz M.M., Marden J.J., Zhou W., Zhang Y., Williams A., Sharov V.S., Nelson K., Luo M., Paulson H., Schöneich C., Engelhardt J.F. john-engelhardt@uiowa.edu Accepted 2013-MAY-17 by ORCHARD 17-Jun-2013: Contacted by IntAct-Help. J. Clin. Invest. (0021-9738) 2008 Only protein-protein interactions imex curation SOD1 mutations disrupt redox-sensitive Rac regulation of NADPH oxidase in a familial ALS model. SOD1 mutations disrupt redox-sensitive Rac regulation of NADPH oxidase in a familial ALS model. J. Clin. Invest. (0021-9738) 2008 imex curation Harraz M.M., Marden J.J., Zhou W., Zhang Y., Williams A., Sharov V.S., Nelson K., Luo M., Paulson H., Schöneich C., Engelhardt J.F. john-engelhardt@uiowa.edu 17-Jun-2013: Contacted by IntAct-Help. Only protein-protein interactions mouse-heart Mus musculus heart [Cardiac; Cardiac tissue] heart Heart [Cardiac; Cardiac tissue] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Harraz M.M., Marden J.J., Zhou W., Zhang Y., Williams A., Sharov V.S., Nelson K., Luo M., Paulson H., Schöneich C., Engelhardt J.F. john-engelhardt@uiowa.edu Accepted 2013-MAY-17 by ORCHARD 17-Jun-2013: Contacted by IntAct-Help. J. Clin. Invest. (0021-9738) 2008 Only protein-protein interactions imex curation SOD1 mutations disrupt redox-sensitive Rac regulation of NADPH oxidase in a familial ALS model. SOD1 mutations disrupt redox-sensitive Rac regulation of NADPH oxidase in a familial ALS model. J. Clin. Invest. (0021-9738) 2008 imex curation Harraz M.M., Marden J.J., Zhou W., Zhang Y., Williams A., Sharov V.S., Nelson K., Luo M., Paulson H., Schöneich C., Engelhardt J.F. john-engelhardt@uiowa.edu 17-Jun-2013: Contacted by IntAct-Help. Only protein-protein interactions mouse-liver Mus musculus liver liver anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Harraz M.M., Marden J.J., Zhou W., Zhang Y., Williams A., Sharov V.S., Nelson K., Luo M., Paulson H., Schöneich C., Engelhardt J.F. john-engelhardt@uiowa.edu Accepted 2013-MAY-17 by ORCHARD 17-Jun-2013: Contacted by IntAct-Help. J. Clin. Invest. (0021-9738) 2008 Only protein-protein interactions imex curation SOD1 mutations disrupt redox-sensitive Rac regulation of NADPH oxidase in a familial ALS model. SOD1 mutations disrupt redox-sensitive Rac regulation of NADPH oxidase in a familial ALS model. J. Clin. Invest. (0021-9738) 2008 imex curation Harraz M.M., Marden J.J., Zhou W., Zhang Y., Williams A., Sharov V.S., Nelson K., Luo M., Paulson H., Schöneich C., Engelhardt J.F. john-engelhardt@uiowa.edu 17-Jun-2013: Contacted by IntAct-Help. Only protein-protein interactions mouse-fibroblast Mus musculus fibroblast Fibroblast cosedimentation cosedimentation western blot western blot Immuno blot Immuno blot Harraz M.M., Marden J.J., Zhou W., Zhang Y., Williams A., Sharov V.S., Nelson K., Luo M., Paulson H., Schöneich C., Engelhardt J.F. john-engelhardt@uiowa.edu Accepted 2013-MAY-17 by ORCHARD 17-Jun-2013: Contacted by IntAct-Help. J. Clin. Invest. (0021-9738) 2008 Only protein-protein interactions imex curation sodc_bovin Superoxide dismutase [Cu-Zn] SOD1 protein protein bovin Bos taurus (Bovine) Bovine MATKAVCVLKGDGPVQGTIHFEAKGDTVVVTGSITGLTEGDHGFHVHQFGDNTQGCTSAGPHFNPLSKKHGGPKDEERHVGDLGNVTADKNGVAIVDIVDPLISLSGEYSIIGRTMVVHEKPDDLGRGGNEESTKTGNAGSRLACGVIGIAK A467EE17E4C31CCD sodc_human Superoxide dismutase [Cu-Zn] Superoxide dismutase 1 SOD1 protein protein human Homo sapiens Human MATKAVCVLKGDGPVQGIINFEQKESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGPHFNPLSRKHGGPKDEERHVGDLGNVTADKDGVADVSIEDSVISLSGDHCIIGRTLVVHEKADDLGKGGNEESTKTGNAGSRLACGVIGIAQ DIP protein P00441 original sequence version: 194 DIP protein P00441 original sequence version: 189 DIP protein P00441 original sequence version: 186 DIP protein P00441 original sequence version: 147 DIP protein P00441 original sequence version: 148 DIP protein P00441 original sequence version: 145 DIP protein P00441 original sequence version: 169 25CA38DA8D564483 rac1_bovin Ras-related C3 botulinum toxin substrate 1 RAC1 p21-Rac1 protein protein bovin Bos taurus (Bovine) Bovine MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL ACEDF83A45E5EA67 rac1_human Ras-related C3 botulinum toxin substrate 1 RAC1 MIG5 p21-Rac1 Ras-like protein TC25 Cell migration-inducing gene 5 protein TC25 protein protein human Homo sapiens Human MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL ACEDF83A45E5EA67 eea1_mouse Early endosome antigen 1 Eea1 protein protein mouse Mus musculus Mouse MFRRILQRTPGRVGSQGSDLDSSATPINTVDVNNESSSEGFICPQCMKSLGSADELFKHYQAVHDAGNDSGHGGEAGLALTRDDITLLRQEVQDLQASLKEEKWYSEELKKELEKYQGLQQQEAKSDGLVTDSSAELQALEQQLEEAQTENFNIKQMKDLFEQKAAQLATEIADIKSKYDEEKSLRAAAEQKVTHLTEDLNKQTTVIQDLKTELLQRPGIEDVAVLKKELVQVQTLMDNMTLERERESEKLKDECKKLQSEHAHLEATINQLRSELAKGPQEVAVYVQEIQKLKGSINELTQKNQNLTEKLQKKDLDYTHLEEKHNEESASRKTLQASLHQRDLDCQQLQARLTASESSLQRAQGELSEKAEAAQKLREELREVESTRQHLKVEVKQLQQQREEKEQHGLQLQGEVSQLHCKLLETERQLGEAHGRLKEQRQLSSEKLMEKEQQVADLQLKLSRLEEQLKEKVTNSTELQHQLEKSKQQHQEQQALQQSATAKLREAQNDLEQVLRQIGDKDQKIQNLEALLQKGKESVSLLEKEREDLYAKIQAGEGETAVLNQLQEKNHALQQQLTQLTEKLKNQSESHKQAEENLHDQVQEQKAHLRAAQDRVLSLETSVSELSSQLNESKEKVSQLDIQIKAKTELLLSAEAAKAAQRADLQNHLDTAQHALQDKQQELNKVSVQLDQLTAKFQEKQEHCIQLESHLKDHKEKHLSLEQKVEDLEGHIKKLEADALEVKASKEQALQSLQQQRQLSTDLELRNAELSRELQEQEEVVSCTKLDLQNKSEILENIKQTLTKKEEENVVLKQEFEKLSQDSKTQHKELGDRMQAAVTELTAVKAQKDALLAELSTTKEKLSKVSDSLKNSKSEFEKENQKGKAAVLDLEKACKELKHQLQVQAESALKEQEDLKKSLEKEKETSQQLKIELNSVKGEVSQAQNTLKQKEKDEQQLQGTINQLKQSAEQKKKQIEALQGEVKNAVSQKTVLENKLQQQSSQAAQELAAEKGKLSALQSNYEKCQADLKQLQSDLYGKESELLATRQDLKSVEEKLTLAQEDLISNRNQIGNQNKSIQELQAAKASLEQDSAKKEALLKEQSKALEDAQREKSVKEKELVAEKSKLAEMEEIKCRQEKEITKLNEELKSHKQESIKEITNLKDAKQLLIQQKLELQGRVDSLKAALEQEKESQQLMREQVKKEEEKRKEEFSEKEAKLHSEIKEKEAGMKKHEENEAKLTMQVTTLNENLGTVKKEWQSSQRRVSELEKQTDDLRGEIAVLEATVQNNQDERRALLERCLKGEGEIEKLQTKALELQRKLDNTTAAVQELGRENQSLQIKHTQALNRKWAEDNEVQNCMSCGKCFSVTVRRHHCRQCGNIFCAECSTKNALTPSSKKPVRVCDACFNDLQG 2365A51EF92019FD cy24b_mouse Cytochrome b-245 heavy chain Cybb Cgd CGD91-phox Cytochrome b(558) subunit beta Heme-binding membrane glycoprotein gp91phox Neutrophil cytochrome b 91 kDa polypeptide gp91-1 gp91-phox p22 phagocyte B-cytochrome protein protein mouse Mus musculus Mouse MGNWAVNEGLSIFVILVWLGLNVFLFINYYKVYDDGPKYNYTRKLLGSALALARAPAACLNFNCMLILLPVCRNLLSFLRGSSACCSTRIRRQLDRNLTFHKMVAWMIALHTAIHTIAHLFNVEWCVNARVGISDRYSIALSDIGDNENEEYLNFAREKIKNPEGGLYVAVTRLAGITGIVITLCLILIITSSTKTIRRSYFEVFWYTHHLFVIFFIGLAIHGAERIVRGQTAESLEEHNLDICADKIEEWGKIKECPVPKFAGNPPMTWKWIVGPMFLYLCERLVRFWRSQQKVVITKVVTHPFKTIELQMKKKGFKMEVGQYIFVKCPKVSKLEWHPFTLTSAPEEDFFSIHIRIVGDWTEGLFNACGCDKQEFQDAWKLPKIAVDGPFGTASEDVFSYEVVMLVGAGIGVTPFASILKSVWYKYCDNATSLKLKKIYFYWLCRDTHAFEWFADLLQLLETQMQERNNANFLSYNIYLTGWDESQANHFAVHHDEEKDVITGLKQKTLYGRPNWDNEFKTIASEHPNTTIGVFLCGPEALAETLSKQSISNSESGPRGVHFIFNKENF DIP protein Q61093 original sequence version: 110 8816274E3D253DFA sodc_mouse Superoxide dismutase [Cu-Zn] Sod1 protein protein mouse Mus musculus Mouse MAMKAVCVLKGDGPVQGTIHFEQKASGEPVVLSGQITGLTEGQHGFHVHQYGDNTQGCTSAGPHFNPHSKKHGGPADEERHVGDLGNVTAGKDGVANVSIEDRVISLSGEHSIIGRTMVVHEKQDDLGKGGNEESTKTGNAGSRLACGVIGIAQ DIP protein P08228 original sequence version: 124 CAE548C66043BAC4 rac1_mouse Ras-related C3 botulinum toxin substrate 1 Rac1 p21-Rac1 protein protein mouse Mus musculus Mouse MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL DIP protein P63001 original sequence version: 125 ACEDF83A45E5EA67 rac1-sod1 4 13 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait physical association physical association 2A rac1-sod1-1 3 13 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait physical association physical association 2A rac1-sod1-2 5 14 unspecified role unspecified role bait bait 13 unspecified role unspecified role prey prey physical association physical association 2A rac1-sod1-3 1 14 unspecified role unspecified role bait bait 13 unspecified role unspecified role prey prey physical association physical association 2A rac1-sod1-4 2 9 unspecified role unspecified role bait bait purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined GTP-bound form 7 unspecified role unspecified role prey prey purified purified direct interaction direct interaction 2B, C, D and E, 3E rac1-sod1-5 2 9 unspecified role unspecified role bait bait purified purified region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined GTP-bound form 7 unspecified role unspecified role prey prey purified purified direct interaction direct interaction 2F cybb-eea1 6 11 unspecified role unspecified role neutral component neutral component 14 unspecified role unspecified role neutral component neutral component 13 unspecified role unspecified role neutral component neutral component 12 unspecified role unspecified role neutral component neutral component colocalization colocalization 3F rac1-sod1-8 2 8 unspecified role unspecified role prey prey purified purified P00441:p.Leu9Gln mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain L Q Wed May 02 16:30:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu9gln' Tue Jun 30 16:51:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu9Gln' P00441:p.Gly11Val mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain G V Wed May 02 17:00:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly11val' Tue Jun 30 17:45:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly11Val' 10 unspecified role unspecified role bait bait purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Prereduced; GTP-bound form. direct interaction direct interaction 3J