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  <entry>
    <source releaseDate="2022-02-03">
      <names>
        <shortLabel>IntAct</shortLabel>
        <fullName>European Bioinformatics Institute</fullName>
        <alias type="synonym" typeAc="MI:1041">IntAct</alias>
        <alias type="synonym" typeAc="MI:1041">intact</alias>
      </names>
      <bibref>
        <xref>
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        </xref>
      </bibref>
      <xref>
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      </xref>
      <attributeList>
        <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/</attribute>
        <attribute name="search-url" nameAc="MI:0615">http://www.ebi.ac.uk/intact/query/${ac}</attribute>
        <attribute name="id-validation-regexp" nameAc="MI:0628">EBI-[0-9]+|IA:[0-9]+</attribute>
        <attribute name="definition">INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions.</attribute>
        <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/intact</attribute>
        <attribute name="postaladdress">European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom</attribute>
        <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/intact/</attribute>
      </attributeList>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>Dynamic regulation of cystic fibrosis transmembrane conductance regulator by competitive interactions of molecular adaptors.</fullName>
        </names>
        <bibref>
          <xref>
            <primaryRef db="pubmed" dbAc="MI:0446" id="17244609" refType="primary-reference" refTypeAc="MI:0358"/>
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          </xref>
          <attributeList>
            <attribute name="publication title" nameAc="MI:1091">Dynamic regulation of cystic fibrosis transmembrane conductance regulator by competitive interactions of molecular adaptors.</attribute>
            <attribute name="journal" nameAc="MI:0885">J. Biol. Chem. (0021-9258)</attribute>
            <attribute name="publication year" nameAc="MI:0886">2007</attribute>
            <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
            <attribute name="author-list" nameAc="MI:0636">Lee J.H., Richter W., Namkung W., Kim K.H., Kim E., Conti M., Lee M.G.</attribute>
            <attribute name="contact-email" nameAc="MI:0634">mlee@yumc.yonsei.ac.kr</attribute>
            <attribute name="author-announcement">13-Jan-2014: Contacted by IntAct-Help.</attribute>
            <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
          </attributeList>
        </bibref>
        <xref>
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          <secondaryRef db="imex" dbAc="MI:0670" id="IM-21824" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <hostOrganismList>
          <hostOrganism ncbiTaxId="9534">
            <names>
              <shortLabel>chlae-cos_7</shortLabel>
              <fullName>Cercopithecus aethiops simian cells transformed with SV40</fullName>
              <alias type="synonym" typeAc="MI:1041">Grivet</alias>
            </names>
            <cellType>
              <names>
                <shortLabel>cos_7</shortLabel>
                <fullName>cercopithecus aethiops, by transformation with SV40</fullName>
              </names>
              <xref>
                <primaryRef db="cabri" dbAc="MI:0246" id="ACC 60" refType="identity" refTypeAc="MI:0356"/>
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            </cellType>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>anti bait coip</shortLabel>
            <fullName>anti bait coimmunoprecipitation</fullName>
            <alias type="synonym" typeAc="MI:1041">anti bait coip</alias>
          </names>
          <xref>
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        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>western blot</shortLabel>
            <fullName>western blot</fullName>
            <alias type="go synonym" typeAc="MI:0303">Immuno blot</alias>
            <alias type="synonym" typeAc="MI:1041">Immuno blot</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0113" refType="identity" refTypeAc="MI:0356"/>
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          </xref>
        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Lee J.H., Richter W., Namkung W., Kim K.H., Kim E., Conti M., Lee M.G.</attribute>
          <attribute name="negative">This experiment relates to a negative interaction.</attribute>
          <attribute name="contact-email" nameAc="MI:0634">mlee@yumc.yonsei.ac.kr</attribute>
          <attribute name="accepted">Accepted 2014-JAN-06 by ORCHARD</attribute>
          <attribute name="journal" nameAc="MI:0885">J. Biol. Chem. (0021-9258)</attribute>
          <attribute name="publication year" nameAc="MI:0886">2007</attribute>
          <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
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          <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
        </attributeList>
      </experimentDescription>
    </experimentList>
    <interactorList>
      <interactor id="2">
        <names>
          <shortLabel>p14270-3</shortLabel>
          <fullName>cAMP-specific 3',5'-cyclic phosphodiesterase 4D</fullName>
          <alias type="isoform synonym" typeAc="MI:0304">PDE3.2</alias>
          <alias type="gene name" typeAc="MI:0301">Pde4d</alias>
          <alias type="gene name synonym" typeAc="MI:0302">DPDE3</alias>
        </names>
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            <shortLabel>protein</shortLabel>
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        </interactorType>
        <organism ncbiTaxId="10116">
          <names>
            <shortLabel>rat</shortLabel>
            <fullName>Rattus norvegicus (Rat)</fullName>
            <alias type="synonym" typeAc="MI:1041">Rat</alias>
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        </organism>
        <sequence>MASNKFKRMLNRELTHLSEMSRSGNQVSEYISNTFLDKQHEVEIPSPTQKEKEKKKRPMSQISGVKKLMHSSSLTNSCIPRFGVKTEQEDVLAKELEDVNKWGLHVFRIAELSGNRPLTVIMHTIFQERDLLKTFKIPVDTLITYLMTLEDHYHADVAYHNNIHAADVVQSTHVLLSTPALEAVFTDLEILAAIFASAIHDVDHPGVSNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDIFQNLTKKQRQSLRKMAIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLQNMVHCADLSNPTKPLQLYRQWTDRIMEEFFRQGDRERERGMEISPMCDKHNASVEKSQVGFIDYIVHPLWETWADLVHPDAQDILDTLEDNREWYQSTIPQSPSPAPDDQEDGRQGQTEKFQFELTLEEDGESDTEKDSGSQVEEDTSCSDSKTLCTQDSESTEIPLDEQVEEEAVAEEESQPQTGVADDCCPDT</sequence>
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          <attribute name="crc64">7DCA5A564384FC34</attribute>
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      </interactor>
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          <shortLabel>p14270-7</shortLabel>
          <fullName>cAMP-specific 3',5'-cyclic phosphodiesterase 4D</fullName>
          <alias type="isoform synonym" typeAc="MI:0304">PDE4D6</alias>
          <alias type="gene name" typeAc="MI:0301">Pde4d</alias>
          <alias type="gene name synonym" typeAc="MI:0302">DPDE3</alias>
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            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
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          <names>
            <shortLabel>rat</shortLabel>
            <fullName>Rattus norvegicus (Rat)</fullName>
            <alias type="synonym" typeAc="MI:1041">Rat</alias>
          </names>
        </organism>
        <sequence>MPEANYLLSVSWGYIKFKRMLNRELTHLSEMSRSGNQVSEYISNTFLDKQHEVEIPSPTQKEKEKKKRPMSQISGVKKLMHSSSLTNSCIPRFGVKTEQEDVLAKELEDVNKWGLHVFRIAELSGNRPLTVIMHTIFQERDLLKTFKIPVDTLITYLMTLEDHYHADVAYHNNIHAADVVQSTHVLLSTPALEAVFTDLEILAAIFASAIHDVDHPGVSNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDIFQNLTKKQRQSLRKMAIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLQNMVHCADLSNPTKPLQLYRQWTDRIMEEFFRQGDRERERGMEISPMCDKHNASVEKSQVGFIDYIVHPLWETWADLVHPDAQDILDTLEDNREWYQSTIPQSPSPAPDDQEDGRQGQTEKFQFELTLEEDGESDTEKDSGSQVEEDTSCSDSKTLCTQDSESTEIPLDEQVEEEAVAEEESQPQTGVADDCCPDT</sequence>
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          <attribute name="crc64">251BF853D7CE6870</attribute>
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      </interactor>
      <interactor id="4">
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          <shortLabel>shan2_rat</shortLabel>
          <fullName>SH3 and multiple ankyrin repeat domains protein 2</fullName>
          <alias type="gene name synonym" typeAc="MI:0302">Cortactin-binding protein 1</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Proline-rich synapse-associated protein 1</alias>
          <alias type="gene name synonym" typeAc="MI:0302">GKAP/SAPAP-interacting protein</alias>
          <alias type="gene name synonym" typeAc="MI:0302">SPANK-3</alias>
          <alias type="gene name" typeAc="MI:0301">Shank2</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Cortbp1</alias>
        </names>
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        </interactorType>
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            <shortLabel>rat</shortLabel>
            <fullName>Rattus norvegicus (Rat)</fullName>
            <alias type="synonym" typeAc="MI:1041">Rat</alias>
          </names>
        </organism>
        <sequence>MKSLLNAFTKKEVPFREAPAYSNRRRRPPNTLAAPRVLLRSNSDNNLNAGAPEWAVCSAATSHRSLSPQLLQQTPSKPDGATKSLGSYAPGPRSRSPSLNRLGGAGEDGKRPQPPHWHVGSPFTPGANKDSLSTFEYPGPRRKLYSAVPGRLFVAIKPYQPQVDGEIPLHRGDRVKVLSIGEGGFWEGSARGHIGWFPAECVEEVQCKPRDSQAETRADRSKKLFRHYTVGSYDSFDAASDCIIEDKTVVLQKKDNEGFGFVLRGAKADTPIEEFTPTPAFPALQYLESVDEGGVAWQAGLRTGDFLIEVNNENVVKVGHRQVVNMIRQGGNHLVLKVVTVTRNLDPDDTARKKAPPPPKRAPTTALTLRSKSMTAELEELGLSLVDKASVRKKKDKPEEIVPASKPSRTAENVAIESRVATIKQRPTSRCFPAASDVNSVYERQGIAVMTPTVPGSPKGPFLGLPRGTMRRQKSIDSRIFLSGITEEERQFLAPPMLKFTRSLSMPDTSEDIPPPPQSVPPSPPPPSPTTYNCPRSPTPRVYGTIKPAFNQNPVAKVPPATRSDTVATMMREKGMFYRRELDRFSLDSEDVYSRSPAPQAAFRTKRGQMPENPYSEVGKIASKAVYVPAKPARRKGMLVKQSNVEDSPEKTCSIPIPTIIVKEPSTSSSGKSSQGSSMEIDPQATEPGQLRPDDSLTVSSPFAAAIAGAVRDREKRLEARRNSPAFLSTDLGDEDVGLGPPAPRMQPSKFPEEGGFGDEDETEQPLLPTPGAAPRELENHFLGGGEAGAQGEAGGPLSSTSKAKGPESGPAAALKSSSPASPENYVHPLTGRLLDPSSPLALALSARDRAMQESQQGHKGEAPKADLNKPLYIDTKMRPSVESGFPPVTRQNTRGPLRRQETENKYETDLSKDRRADDKKNMLINIVDTAQQKSAGLLMVHTVDIPVAGPPLEEEEDREDGDTKPDHSPSTVPEGVPKTEGALQISAAPEPAAAPGRTIVAAGSVEEAVILPFRIPPPPLASVDLDEDFLFTEPLPPPLEFANSFDIPDDRAASVPALADLVKQKKSDTPQPPSLNSSQPANSTDSKKPAGISNCLPSSFLPPPESFDAVTDSGIEEVDSRSSSDHHLETTSTISTVSSISTLSSEGGESMDTCTVYADGQAFVVDKPPVPPKPKMKPIVHKSNALYQDTLPEEDTDGFVIPPPAPPPPPGSAQAGVAKVIQPRTSKLWGDVTEVKSPILSGPKANVISELNSILQQMNRGKSVKPGEGLELPVGAKSANLAPRSPEVMSTVSGTRSTTVTFTVRPGTSQPITLQSRPPDYESRTSGPRRAPSPVVSPTELSKEILPTPPSAAAASPSPTLSDVFSLPSQSPAGDLFGLNPAGRSRSPSPSILQQPISNKPFTTKPVHLWTKPDVADWLESLNLGEHKETFMDNEIDGSHLPNLQKEDLIDLGVTRVGHRMNIERALKQLLDR</sequence>
        <attributeList>
          <attribute name="crc64">F503D44D0D9AB7C1</attribute>
        </attributeList>
      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="5">
        <names>
          <shortLabel>pde4d-shank2-4</shortLabel>
        </names>
        <xref>
          <primaryRef db="intact" dbAc="MI:0469" id="EBI-8790600" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-8790574" refType="see-also" refTypeAc="MI:0361"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-8790625" refType="see-also" refTypeAc="MI:0361"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-8790629" refType="see-also" refTypeAc="MI:0361"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-8790654" refType="see-also" refTypeAc="MI:0361"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-9032523" refType="see-also" refTypeAc="MI:0361"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-8790671" refType="see-also" refTypeAc="MI:0361"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-9032559" refType="see-also" refTypeAc="MI:0361"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="6">
            <interactorRef>2</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>bait</shortLabel>
                  <fullName>bait</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0496" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-49" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
          </participant>
          <participant id="7">
            <interactorRef>4</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>prey</shortLabel>
                  <fullName>prey</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0498" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-58" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>physical association</shortLabel>
            <fullName>physical association</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0915" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1813147" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
        <negative>true</negative>
        <attributeList>
          <attribute name="figure legend" nameAc="MI:0599">Fig. 4d</attribute>
        </attributeList>
      </interaction>
      <interaction id="8">
        <names>
          <shortLabel>pde4d-shank2-5</shortLabel>
        </names>
        <xref>
          <primaryRef db="intact" dbAc="MI:0469" id="EBI-8790605" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-9032559" refType="see-also" refTypeAc="MI:0361"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-8790654" refType="see-also" refTypeAc="MI:0361"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-8790574" refType="see-also" refTypeAc="MI:0361"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-9032523" refType="see-also" refTypeAc="MI:0361"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-8790671" refType="see-also" refTypeAc="MI:0361"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-8790629" refType="see-also" refTypeAc="MI:0361"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-8790625" refType="see-also" refTypeAc="MI:0361"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="9">
            <interactorRef>3</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>bait</shortLabel>
                  <fullName>bait</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0496" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-49" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
          </participant>
          <participant id="10">
            <interactorRef>4</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>prey</shortLabel>
                  <fullName>prey</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0498" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-58" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>physical association</shortLabel>
            <fullName>physical association</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0915" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1813147" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
        <negative>true</negative>
        <attributeList>
          <attribute name="figure legend" nameAc="MI:0599">Fig. 4d</attribute>
        </attributeList>
      </interaction>
    </interactionList>
  </entry>
</entrySet>