mbinfo MBInfo http://www.mechanobio.info/ Molecular mapping of tyrosine-phosphorylated proteins in focal adhesions using fluorescence resonance energy transfer. Molecular mapping of tyrosine-phosphorylated proteins in focal adhesions using fluorescence resonance energy transfer. J. Cell. Sci. (0021-9533) 2006 imex curation Ballestrem C., Erez N., Kirchner J., Kam Z., Bershadsky A., Geiger B. Alternative method for quantitating the FRET measurements were carried out (Fig 2A), where dequenching of the donor fluorophore following photobleaching of the acceptor was performed. Experiments involving FAK (Fig 3A, 3B, 3C, 4F, 7, 8) could not be curated since the source of FAK could not be identified or traced. benny.geiger@weizmann.ac.il 01-March-2013: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Ballestrem C., Erez N., Kirchner J., Kam Z., Bershadsky A., Geiger B. Alternative method for quantitating the FRET measurements were carried out (Fig 2A), where dequenching of the donor fluorophore following photobleaching of the acceptor was performed. Experiments involving FAK (Fig 3A, 3B, 3C, 4F, 7, 8) could not be curated since the source of FAK could not be identified or traced. benny.geiger@weizmann.ac.il Accepted 2013-FEB-06 by ORCHARD 01-March-2013: Contacted by IntAct-Help. J. Cell. Sci. (0021-9533) 2006 Only protein-protein interactions imex curation Molecular mapping of tyrosine-phosphorylated proteins in focal adhesions using fluorescence resonance energy transfer. Molecular mapping of tyrosine-phosphorylated proteins in focal adhesions using fluorescence resonance energy transfer. J. Cell. Sci. (0021-9533) 2006 imex curation Ballestrem C., Erez N., Kirchner J., Kam Z., Bershadsky A., Geiger B. Alternative method for quantitating the FRET measurements were carried out (Fig 2A), where dequenching of the donor fluorophore following photobleaching of the acceptor was performed. Experiments involving FAK (Fig 3A, 3B, 3C, 4F, 7, 8) could not be curated since the source of FAK could not be identified or traced. benny.geiger@weizmann.ac.il 01-March-2013: Contacted by IntAct-Help. Only protein-protein interactions mouse-nih_3t3 Mus musculus Swiss NIH 3t3 embryo fibroblast cells nih_3t3 Mouse Swiss NIH embryo contact inhibited fibroblast cells fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag fluorescence tag visualisation by fluorescence tag fluorescence Ballestrem C., Erez N., Kirchner J., Kam Z., Bershadsky A., Geiger B. Alternative method for quantitating the FRET measurements were carried out (Fig 2A), where dequenching of the donor fluorophore following photobleaching of the acceptor was performed. Experiments involving FAK (Fig 3A, 3B, 3C, 4F, 7, 8) could not be curated since the source of FAK could not be identified or traced. benny.geiger@weizmann.ac.il Accepted 2013-FEB-06 by ORCHARD 01-March-2013: Contacted by IntAct-Help. J. Cell. Sci. (0021-9533) 2006 Only protein-protein interactions imex curation Molecular mapping of tyrosine-phosphorylated proteins in focal adhesions using fluorescence resonance energy transfer. Molecular mapping of tyrosine-phosphorylated proteins in focal adhesions using fluorescence resonance energy transfer. J. Cell. Sci. (0021-9533) 2006 imex curation Ballestrem C., Erez N., Kirchner J., Kam Z., Bershadsky A., Geiger B. Alternative method for quantitating the FRET measurements were carried out (Fig 2A), where dequenching of the donor fluorophore following photobleaching of the acceptor was performed. Experiments involving FAK (Fig 3A, 3B, 3C, 4F, 7, 8) could not be curated since the source of FAK could not be identified or traced. benny.geiger@weizmann.ac.il 01-March-2013: Contacted by IntAct-Help. Only protein-protein interactions mouse-nih_3t3 Mus musculus Swiss NIH 3t3 embryo fibroblast cells nih_3t3 Mouse Swiss NIH embryo contact inhibited fibroblast cells fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Ballestrem C., Erez N., Kirchner J., Kam Z., Bershadsky A., Geiger B. Alternative method for quantitating the FRET measurements were carried out (Fig 2A), where dequenching of the donor fluorophore following photobleaching of the acceptor was performed. Experiments involving FAK (Fig 3A, 3B, 3C, 4F, 7, 8) could not be curated since the source of FAK could not be identified or traced. Experiments involving FAK (Fig 3A, 3B, 3C, 4F, 7, 8) could not be curated since the source of FAK could not be identified or traced. benny.geiger@weizmann.ac.il Accepted 2013-FEB-06 by ORCHARD 01-March-2013: Contacted by IntAct-Help. J. Cell. Sci. (0021-9533) 2006 Only protein-protein interactions imex curation Molecular mapping of tyrosine-phosphorylated proteins in focal adhesions using fluorescence resonance energy transfer. Molecular mapping of tyrosine-phosphorylated proteins in focal adhesions using fluorescence resonance energy transfer. J. Cell. Sci. (0021-9533) 2006 imex curation Ballestrem C., Erez N., Kirchner J., Kam Z., Bershadsky A., Geiger B. Alternative method for quantitating the FRET measurements were carried out (Fig 2A), where dequenching of the donor fluorophore following photobleaching of the acceptor was performed. Experiments involving FAK (Fig 3A, 3B, 3C, 4F, 7, 8) could not be curated since the source of FAK could not be identified or traced. benny.geiger@weizmann.ac.il 01-March-2013: Contacted by IntAct-Help. Only protein-protein interactions mouse-nih_3t3 Mus musculus Swiss NIH 3t3 embryo fibroblast cells nih_3t3 Mouse Swiss NIH embryo contact inhibited fibroblast cells anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Ballestrem C., Erez N., Kirchner J., Kam Z., Bershadsky A., Geiger B. Alternative method for quantitating the FRET measurements were carried out (Fig 2A), where dequenching of the donor fluorophore following photobleaching of the acceptor was performed. Experiments involving FAK (Fig 3A, 3B, 3C, 4F, 7, 8) could not be curated since the source of FAK could not be identified or traced. benny.geiger@weizmann.ac.il Accepted 2013-FEB-06 by ORCHARD 01-March-2013: Contacted by IntAct-Help. J. Cell. Sci. (0021-9533) 2006 Only protein-protein interactions imex curation bcar1_rat Breast cancer anti-estrogen resistance protein 1 CRK-associated substrate p130cas Bcar1 Cas Crkas protein protein rat Rattus norvegicus (Rat) Rat MKYLVSVGAGPARRAGGLEDVSWGPRVSRRPQSYRAARHVNESLPRSAFRVPAAHGASVTPSAALGSGLPETQPEAVCRGTEKPRFAEGCKPAASRDKNVLAKALYDNVAESPDELSFRKGDIMTVLERDTQGLDGWWLCSLHGRQGIVPGNRLKILVGMYDKKPAAPGPGPPATPPQPQPSLPQGVHTPVPPASQYSPMLPTAYQPQPDNVYLVPTPSKTQQGLYQAPGPNPQFQSPPAKQTSTFSKQTPHHSFPSPATDLYQVPPGPGSPAQDIYQVPPSAGTGHDIYQVPPSLDTRSWEGTKPPAKVVVPTRVGQGYVYEASQAEQDEYDTPRHLLAPGSQDIYDVPPVRGLLPNQYGQEVYDTPPMAVKGPNGRDPLLDVYDVPPSVEKGLPPSNHHSVYDVPPSVSKDVPDGPLLREETYDVPPAFAKPKPFDPTRHPLILAAPPPDSPPAEDVYDVPPPAPDLYDVPPGLRRPGPGTLYDVPRERVLPPEVADGSVIDDGVYAVPPPAEREAPTDGKRLSASSTGSTRSSQSASSLEVVVPGREPLELEVAVETLARLQQGVSTTVAHLLDLVGSASGPGGWRSTSEPQEPPVQDLKAAVAAVHGAVHELLEFARSAVSSATHTSDRTLHAKLSRQLQKMEDVYQTLVVHGQVLDSGRGGPGFTLDDLDRLVACSRAVPEDAKQLASFLHGNASLLFRRTKAPGPGPEGSSSLHLNPTDKASSIQSRPLPSPPKFTSQDSPDGQYENSEGGWMEDYDYVHLQGKEEFEKTQKELLEKGNIVRQGKGQLELQQLKQFERLEQEVSRPIDHDLANWTPAQPLVPGRTGGLGPSDRQLLLFYLEQCEANLTTLTDAVDAFFTAVATNQPPKIFVAHSKFVILSAHKLVFIGDTLSRQAKAADVRSKVTHYSNLLCDLLRGIVATTKAAALQYPSPSAAQDMVDRVKELGHSTQQFRRVLGQLAAA E861641BFD68D377 paxi_human Paxillin PXN protein protein human Homo sapiens Human MDDLDALLADLESTTSHISKRPVFLSEETPYSYPTGNHTYQEIAVPPPVPPPPSSEALNGTILDPLDQWQPSSSRFIHQQPQSSSPVYGSSAKTSSVSNPQDSVGSPCSRVGEEEHVYSFPNKQKSAEPSPTVMSTSLGSNLSELDRLLLELNAVQHNPPGFPADEANSSPPLPGALSPLYGVPETNSPLGGKAGPLTKEKPKRNGGRGLEDVRPSVESLLDELESSVPSPVPAITVNQGEMSSPQRVTSTQQQTRISASSATRELDELMASLSDFKIQGLEQRADGERCWAAGWPRDGGRSSPGGQDEGGFMAQGKTGSSSPPGGPPKPGSQLDSMLGSLQSDLNKLGVATVAKGVCGACKKPIAGQVVTAMGKTWHPEHFVCTHCQEEIGSRNFFERDGQPYCEKDYHNLFSPRCYYCNGPILDKVVTALDRTWHPEHFFCAQCGAFFGPEGFHEKDGKAYCRKDYFDMFAPKCGGCARAILENYISALNTLWHPECFVCRECFTPFVNGSFFEHDGQPYCEVHYHERRGSLCSGCQKPITGRCITAMAKKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCQNCFLKLFC DIP protein P49023 original sequence version: 115 DIP protein P49023 original sequence version: 126 DIP protein P49023 original sequence version: 169 DIP protein P49023 original sequence version: 140 DIP protein P49023 original sequence version: 175 ABF6C0BE5939623F bcar1_mouse Breast cancer anti-estrogen resistance protein 1 Bcar1 Cas Crkas p130cas CRK-associated substrate protein protein mouse Mus musculus Mouse MTVPNVLAKALYDNVAESPDELSFRKGDIMTVLERDTQGLDGWWLCSLHGRQGIVPGNRLKILVGMYDKKPVGPGPGPPATPPQPQPSLPQGVHAPVPPASQYSPMLPTAYQPQSDNVYLVPTPSKTQQGLYQAPGPNPQFQSPPAKQTSTFSKQTPHHSFPSPATDLYQVPPGPGSPAQDIYQVPPSAGIGHDIYQVPPSLDTRGWEGTKPPAKVVVPTRVGQGYVYEAAQTEQDEYDTPRHLLAPGPQDIYDVPPVRGLLPNQYGQEVYDTPPMAVKGPNGRDPLLDVYDVPPSVEKGLLSSSHHSVYDVPPSVSKDVPDGPLLREETYDVPPAFAKPKPFDPTRHPLILAAPPPDSPAAEDVYDVPPPAPDLYDVPPGLRRPGPGTLYDVPRERVLPPEVADGSVVDDGVYAVPPPAEREAPTDGKRLSASSTGSTRSSQSASSLEVVVPGREPLELEVAVESLARLQQGVSTTVAHLLDLVGSASGPGGWRGTSEPQEPPAQDLKAAVAAVHGAVHELLEFARGAVSNATHTSDRTLHAKLSRQLQKMEDVYQTLVVHGQVLDSGRGSPGFTPEDLDRLVACSRAVPEDAKQLASFLHGNASLLFRRTKAPGPGPEGSSSLHPNPTDKASSIQSRPLPSPPKFTSQDSPDGQYENSEGGWMEDYDYVHLQGKEEFEKTQKELLERGNIMRQGKGQLELQQLKQFERLEQEVSRPIDHDLANWTPAQPLVPGRTGGLGPSDRQLLLFYLEQCEANLTTLTDAVDAFFTAVATNQPPKIFVAHSKFVILSAHKLVFIGDTLSRQAKAADVRSQVTHYSNLLCDLLRGIVATTKAAALQYPSPSAAQDMVDRVKELGHSTQQFRRVLGQLAAA 81E56BC7AD87B095 paxi_mouse Paxillin Pxn protein protein mouse Mus musculus Mouse MDDLDALLADLESTTSHISKRPVFLSEEPPYSYPTGNHTYQEIAVPPPVPPPPSSEALNGTVLDPLDQWQPSGSRYAHQQPPSPLPVYSSSAKNSSASNTQDGVGSLCSRAGEEEHVYSFPNKQKSAEPSPTVMSSSLGSNLSELDRLLLELNAVQHSPPGFPADEAESSPPLPGALSPLYGIPENNTPLGGKAGPLVKEKPKRNGGRGLEDVRPSVESLLDELESSVPSPVPAITVNQGEMSSPQRVTSSQQQTRISASSATRELDELMASLSDFKMQGLEQRVDGERPWAAGWPPSSRQSSPEGQDEGGFMAQGKTGSSSPPGGLSKPGSQLDSMLGSLQSDLNKLGVATVAKGVCGACKKPIAGQVVTAMGKTWHPEHFVCTHCQEEIGSRNFFERDGQPYCEKDYHSLFSPRCYYCNGPILDKVVTALDRTWHPEHFFCAQCGAFFGPEGFHEKDGKAYCRKDYFDMFAPKCGGCARAILENYISALNTLWHPECFVCRECFTPFVNGSFFEHDGQPYCEVHYHERRGSLCSGCQKPITGRCITAMAKKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCQSCFVKLFC B41A1892E6F60544 src_mouse Neuronal proto-oncogene tyrosine-protein kinase Src Src pp60c-src Proto-oncogene c-Src protein protein mouse Mus musculus Mouse MGSNKSKPKDASQRRRSLEPSENVHGAGGAFPASQTPSKPASADGHRGPSAAFVPPAAEPKLFGGFNSSDTVTSPQRAGPLAGGVTTFVALYDYESRTETDLSFKKGERLQIVNNTEGDWWLAHSLSTGQTGYIPSNYVAPSDSIQAEEWYFGKITRRESERLLLNAENPRGTFLVRESETTKGAYCLSVSDFDNAKGLNVKHYKIRKLDSGGFYITSRTQFNSLQQLVAYYSKHADGLCHRLTTVCPTSKPQTQGLAKDAWEIPRESLRLEVKLGQGCFGEVWMGTWNGTTRVAIKTLKPGTMSPEAFLQEAQVMKKLRHEKLVQLYAVVSEEPIYIVTEYMNKGSLLDFLKGETGKYLRLPQLVDMSAQIASGMAYVERMNYVHRDLRAANILVGENLVCKVADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELTTKGRVPYPGMVNREVLDQVERGYRMPCPPECPESLHDLMCQCWRKEPEERPTFEYLQAFLEDYFTSTEPQYQPGENL DIP protein P05480 original sequence version: 163 DIP protein P05480 original sequence version: 99 DIP protein P05480 original sequence version: 128 DIP protein P05480 original sequence version: 136 DIP protein P05480 original sequence version: 176 22CB5C6BE7A061C0 pxn-src-1 2 6 unspecified role unspecified role fluorescence donor fluorescence donor region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept sh2 domain sufficient to bind sufficient binding region sufficient to bind range range range range Two tandem repeats of the SH2 domain of pp60c-src fused to YFP was used as a PY reporter. The sequence range [156-253] is not directly referenced by the authors and is inferred from uniprot. region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined proximity proximity close-contact colocalization neighbourhood interaction Fig 1A, 1B, 1D, 2A, 3B, 5A, 6A, 6B, 6C, 8B. The FRET values betwwen CFP-Paxillin and YFP-Src-SH2 remained high in Y27632 (Rock inhibitor)-treated cells (Fig 6A) and in the presence of activated Rac (Figs 6B, 6C) and Rho (Fig 6C). src-pxn 4 8 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined sh2 domain sufficient to bind sufficient binding region sufficient to bind range range range range Tandem repeats of the pp60c-src SH2 domain fused to YFP was used as PY reporter. The sequence range [156-253] was not directly referenced by the author and was inferred from uniprot. physical association physical association Fig 1C (Right bottom panel). bcar1-src-3 2 5 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role fluorescence donor fluorescence donor region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined sh2 domain sufficient to bind sufficient binding region sufficient to bind range range range range Tandem repeats of the pp60c-src fused to the CFP tag was used as a PY reporter. The sequence range [156-253] was not directly referenced by the author and was inferred from uniprot. proximity proximity close-contact colocalization neighbourhood interaction Fig 3A, 3B. bcar1-src-2 1 9 unspecified role unspecified role prey prey region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined sh2 domain sufficient to bind sufficient binding region sufficient to bind range range range range Tandem repeats of pp60c-src SH2 domain fused to CFP, was used as PY reporter. The sequence range [156-253] was not directly referenced by the author and was inferred from uniprot. 5 unspecified role unspecified role bait bait region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 3C (left). bcar1-pxn 3 8 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig 4I. pxn-src-4 2 6 unspecified role unspecified role neutral component neutral component region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role neutral component neutral component sh2 domain sufficient to bind sufficient binding region sufficient to bind range range range range Two tandem repeats of the SH2 domain of pp60c-src fused to YFP was used as a PY reporter. The sequence range [156-253] is not directly referenced by the authors and is inferred from uniprot. region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined proximity proximity close-contact colocalization neighbourhood interaction Fig 1A, 1B.