HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html PKC beta regulates BCR-mediated IKK activation by facilitating the interaction between TAK1 and CARMA1. PKC beta regulates BCR-mediated IKK activation by facilitating the interaction between TAK1 and CARMA1. J. Exp. Med. (0022-1007) 2005 imex curation Shinohara H., Yasuda T., Aiba Y., Sanjo H., Hamadate M., Watarai H., Sakurai H., Kurosaki T. Mouse primary B cell from spleen of C57BL/6J kurosaki@rcai.riken.jp 8-May-2012: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro protein kinase assay protein kinase assay western blot western blot Immuno blot Immuno blot Shinohara H., Yasuda T., Aiba Y., Sanjo H., Hamadate M., Watarai H., Sakurai H., Kurosaki T. Mouse primary B cell from spleen of C57BL/6J kurosaki@rcai.riken.jp Accepted 2013-APR-30 by ORCHARD 8-May-2012: Contacted by IntAct-Help. J. Exp. Med. (0022-1007) 2005 imex curation PKC beta regulates BCR-mediated IKK activation by facilitating the interaction between TAK1 and CARMA1. PKC beta regulates BCR-mediated IKK activation by facilitating the interaction between TAK1 and CARMA1. J. Exp. Med. (0022-1007) 2005 imex curation Shinohara H., Yasuda T., Aiba Y., Sanjo H., Hamadate M., Watarai H., Sakurai H., Kurosaki T. Mouse primary B cell from spleen of C57BL/6J kurosaki@rcai.riken.jp 8-May-2012: Contacted by IntAct-Help. Only protein-protein interactions imex curation chick-dt40 Gallus gallus bursa lymphoma dt40 chicken bursa lymphoma http://www.lgcpromochem-atcc.com/common/catalog/numSearch/numResults.cfm chicken bursa lymphoma anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Shinohara H., Yasuda T., Aiba Y., Sanjo H., Hamadate M., Watarai H., Sakurai H., Kurosaki T. Mouse primary B cell from spleen of C57BL/6J kurosaki@rcai.riken.jp Accepted 2013-APR-30 by ORCHARD 8-May-2012: Contacted by IntAct-Help. J. Exp. Med. (0022-1007) 2005 imex curation PKC beta regulates BCR-mediated IKK activation by facilitating the interaction between TAK1 and CARMA1. PKC beta regulates BCR-mediated IKK activation by facilitating the interaction between TAK1 and CARMA1. J. Exp. Med. (0022-1007) 2005 imex curation Shinohara H., Yasuda T., Aiba Y., Sanjo H., Hamadate M., Watarai H., Sakurai H., Kurosaki T. Mouse primary B cell from spleen of C57BL/6J kurosaki@rcai.riken.jp 8-May-2012: Contacted by IntAct-Help. Only protein-protein interactions imex curation chick-dt40 Gallus gallus bursa lymphoma dt40 chicken bursa lymphoma http://www.lgcpromochem-atcc.com/common/catalog/numSearch/numResults.cfm chicken bursa lymphoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Shinohara H., Yasuda T., Aiba Y., Sanjo H., Hamadate M., Watarai H., Sakurai H., Kurosaki T. Mouse primary B cell from spleen of C57BL/6J kurosaki@rcai.riken.jp Accepted 2013-APR-30 by ORCHARD 8-May-2012: Contacted by IntAct-Help. J. Exp. Med. (0022-1007) 2005 imex curation PKC beta regulates BCR-mediated IKK activation by facilitating the interaction between TAK1 and CARMA1. PKC beta regulates BCR-mediated IKK activation by facilitating the interaction between TAK1 and CARMA1. J. Exp. Med. (0022-1007) 2005 imex curation Shinohara H., Yasuda T., Aiba Y., Sanjo H., Hamadate M., Watarai H., Sakurai H., Kurosaki T. Mouse primary B cell from spleen of C57BL/6J kurosaki@rcai.riken.jp 8-May-2012: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-b-cell Mus musculus B-cell b-cell B-cell [B lymphocyte; B-lymphocyte; B cell; B-cells] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Shinohara H., Yasuda T., Aiba Y., Sanjo H., Hamadate M., Watarai H., Sakurai H., Kurosaki T. Mouse C57BL/6J spleen B cells Mouse primary B cell from spleen of C57BL/6J kurosaki@rcai.riken.jp Accepted 2013-APR-30 by ORCHARD 8-May-2012: Contacted by IntAct-Help. J. Exp. Med. (0022-1007) 2005 imex curation e1c736_chick MALT1 protein protein chick Gallus gallus (Chicken) Chicken AVSRFLQLLEKGVYAIFYYAGHGYEHLGRNYMVPVDAPQLYAPENCISVQRILQKMQQQQTALNLILLDTCRKWYNPECALSQVQPLEPWGNTVYGYATSEDAEAYELQNGEFSSGIFMKYLKKHILQEKKVTHMLEDVLEDLGRDPVVTGKQVMEIKHTLKEARSLTDPICPTGAATERWGCSREPPSETVTFPCGVQAELRFHRLFSNVLSVCAKLQDLPAHLTDAQL The sequence has been withdrawn from uniprot. DA9D2A68CFCCA780 m3k7_mouse Mitogen-activated protein kinase kinase kinase 7 Map3k7 Tak1 Transforming growth factor-beta-activated kinase 1 protein protein mouse Mus musculus Mouse MSTASAASSSSSSSASEMIEAPSQVLNFEEIDYKEIEVEEVVGRGAFGVVCKAKWRAKDVAIKQIESESERKAFIVELRQLSRVNHPNIVKLYGACLNPVCLVMEYAEGGSLYNVLHGAEPLPYYTAAHAMSWCLQCSQGVAYLHSMQPKALIHRDLKPPNLLLVAGGTVLKICDFGTACDIQTHMTNNKGSAAWMAPEVFEGSNYSEKCDVFSWGIILWEVITRRKPFDEIGGPAFRIMWAVHNGTRPPLIKNLPKPIESLMTRCWSKDPSQRPSMEEIVKIMTHLMRYFPGADEPLQYPCQYSDEGQSNSATSTGSFMDIASTNTSNKSDTNMEQVPATNDTIKRLESKLLKNQAKQQSESGRLSLGASRGSSVESLPPTSEGKRMSADMSEIEARIVATAGNGQPRRRSIQDLTVTGTEPGQVSSRSSSPSVRMITTSGPTSEKPARSHPWTPDDSTDTNGSDNSIPMAYLTLDHQLQPLAPCPNSKESMAVFEQHCKMAQEYMKVQTEIALLLQRKQELVAELDQDEKDQQNTSRLVQEHKKLLDENKSLSTYYQQCKKQLEVIRSQQQKRQGTS DIP protein Q62073 original sequence version: 121 DIP protein Q62073 original sequence version: 108 DIP protein Q62073 original sequence version: 151 97C8F6F3C8E283EE car11_mouse Caspase recruitment domain-containing protein 11 Card11 CARD-containing MAGUK protein 1 Carma1 protein protein mouse Mus musculus Mouse MPGGGPAMDDYMETLKDEEEALWDNVECNRHMLSRYINPAKLTPYLRQCKVIDEQDEDEVLNAPMLPSKINRAGRLLDILHTKGQRGYVVFLESLEFYYPELYKLVTGKEPTRRFSTIVVEEGHEGLTHFLMNEVIKLQQQVKAKDLQRCELLAKSRQLEDEKKQLSLIRVELLTFQERYYKMKEERDSYNDELVKVKDDNYNLAMRYAQLSEEKNMAVMRSRDLQLEIDQLKHRLNKMEEECKLERNQSLKLKNDIENRPRKEQVLELERENEMLKTKIQELQSIIQAGKRSLPDSDKAILDILEHDRKEALEDRQELVNKIYNLQEEVRQAEELRDKYLEEKEDLELKCSTLGKDCEMYKHRMNTVMLQLEEVERERDQAFHSRDEAQTQYSQCLIEKDKYRKQIRELEEKNDEMRIEMVRREACIVNLESKLRRLSKDNGSLDQSLPRHLPATIISQNLGDTSPRTNGQEADDSSTSEESPEDSKYFLPYHPPRRRMNLKGIQLQRAKSPISMKQASEFQALMRTVKGHEEDFTDGSPSSSRSLPVTSSFSKMQPHRSRSSIMSITAEPPGNDSIVRRCKEDAPHRSTVEEDNDSCGFDALDLDDENHERYSFGPPSIHSSSSSHQSEGLDAYDLEQVNLMLRKFSLERPFRPSVTSGGHVRGTGPLVQHTTLNGDGLITQLTLLGGNARGSFIHSVKPGSLAERAGLREGHQLLLLEGCIRGERQSVPLDACTKEEARWTIQRCSGLITLHYKVNHEGYRKLLKEMEDGLITSGDSFYIRLNLNISSQLDACSMSLKCDDVVHVLDTMYQDRHEWLCARVDPFTDQDLDTGTIPSYSRAQQLLLVKLQRLVHRGNREEADSAHHTLRSLRNTLQPEEMLSTSDPRVSPRLSRASFFFGQLLQFVSRSENKYKRMNSNERVRIISGSPLGSLSRSSLDATKLLTEKHEELDPENELSRNLTLIPYSLVRAFHCERRRPVLFTPTMLAKTLVQKLLNSGGAMEFTICKSDIVTRDEFLRKQKTETIIYSREKNPNTFECIVPANIEAVAAKNKHCLLEAGIGCVRDLIKCKVYPIVLLIRVSEKNIKRFRKLLPRPETEEEFLRVCRLKEKELEALPCLYATVEAEMWSSVEELLRVLKDKIVEEQRKTIWVDEDQL DIP protein Q8CIS0 original sequence version: 70 2ED53905B99DD94E ikkb_human Inhibitor of nuclear factor kappa-B kinase subunit beta IKBKB IKKB I-kappa-B kinase 2 Nuclear factor NF-kappa-B inhibitor kinase beta Serine/threonine protein kinase IKBKB protein protein human Homo sapiens Human MSWSPSLTTQTCGAWEMKERLGTGGFGNVIRWHNQETGEQIAIKQCRQELSPRNRERWCLEIQIMRRLTHPNVVAARDVPEGMQNLAPNDLPLLAMEYCQGGDLRKYLNQFENCCGLREGAILTLLSDIASALRYLHENRIIHRDLKPENIVLQQGEQRLIHKIIDLGYAKELDQGSLCTSFVGTLQYLAPELLEQQKYTVTVDYWSFGTLAFECITGFRPFLPNWQPVQWHSKVRQKSEVDIVVSEDLNGTVKFSSSLPYPNNLNSVLAERLEKWLQLMLMWHPRQRGTDPTYGPNGCFKALDDILNLKLVHILNMVTGTIHTYPVTEDESLQSLKARIQQDTGIPEEDQELLQEAGLALIPDKPATQCISDGKLNEGHTLDMDLVFLFDNSKITYETQISPRPQPESVSCILQEPKRNLAFFQLRKVWGQVWHSIQTLKEDCNRLQQGQRAAMMNLLRNNSCLSKMKNSMASMSQQLKAKLDFFKTSIQIDLEKYSEQTEFGITSDKLLLAWREMEQAVELCGRENEVKLLVERMMALQTDIVDLQRSPMGRKQGGTLDDLEEQARELYRRLREKPRDQRTEGDSQEMVRLLLQAIQSFEKKVRVIYTQLSKTVVCKQKALELLPKVEEVVSLMNEDEKTVVRLQEKRQKELWNLLKIACSKVRGPVSGSPDSMNASRLSQPGQLMSQPSTASNSLPEPAKKSEELVAEAHNLCTLLENAIQDTVREQDQSFTALDWSWLQTEEEEHSCLEQAS DIP protein O14920 original sequence version: 89 DIP protein O14920 original sequence version: 100 DIP protein O14920 original sequence version: 121 DIP protein O14920 original sequence version: 126 DIP protein O14920 original sequence version: 136 DIP protein O14920 original sequence version: 128 DIP protein O14920-1 original sequence version: 151 DIP protein O14920 original sequence version: 142 DIP protein O14920 original sequence version: 159 DIP protein O14920 original sequence version: 93 DIP protein O14920 original sequence version: 124 DIP protein O14920-1 original sequence version: 178 DIP protein O14920 original sequence version: 85 F9CADF671AE9E14E f1nkg4_chick BCL10 protein protein chick Gallus gallus (Chicken) Chicken SSAVGRPLTEDEMADVKKEALERMRPYLCDKIIAERHFDYLRSKKILTREDTEEISSRSSSRKKTGKLLDYLAENPKGLDALVESIRRERTQNFLLQKITDIVLKVKNEKLEALKGLSCSTCMTSLYGGTNNLSRSYSDESNFFDKTKDKESTQMHNPEEDYSTAAFMSAVSLHSMNLPVAEMGNAESAVFSVTLPGPGDPGAPPLPPELQSEQQEPCTSSSDNCFLPLRSRSLHPQ The sequence has been withdrawn from uniprot. 6E9796811BCDD0FE q5f3w0_chick CARD11 RCJMB04_5k17 protein protein chick Gallus gallus (Chicken) Chicken MSTQGGEPEMDDCLETLKDEEEALWENVECNRHMLSRYINPAKLTPYLRQCKVIDEQDEDEVLNSLMLPSKINRAGRLLDILHTKGQRGYVVFLESLEFYYPELYKLVTGKEPTRRFSTIVVEEGHEGLTHFLMNEIIKLQQQVKTKDMQRCDLLAKSRQLEDERKQLKLNKIELMTFQERYNKMKEERNNYNDELVKVKDENYNLAMRYAQLSDEKSMAVMRSRDLQLEIDQLKHRLNKVEEECKLERNQSLKLKNDIENRPKKEQMLELERENEMMKTKIQELQSIIQADKRSLPDSDKAILDILEHDRKEALEDRHELVNKIFNLQEEIRHVEDLRDKYLEEKEDLELKCSTLGKDCEMYKHRMNTVMIQLEEVEKERDQAFRSRDEAQTQYSHCLIEKDKYRKQIRELEERNDELRIEMVRKEACIVNLECKLRRLSKDNNCHDQSLPRNLPITIISQTFGNSSPKANGQEADDSSTSEDSPEDNKFFLPDQARLKRRVNLKGIQINPRAKSPVSMNRTSEFQAVRAQDEDGANASNGRTDTSSSNSVSISNSISSCEVSKIQTLRNRNDSIMSTTPEPPGNDSIVRRCKEDAPPCSMVEEDNDSFGFDALELDDDSHDRHSHGAPSVHSSSSSHQSEGLDAYDLEHVNSIFRKFSLERPFRPSVTSVGRIRSSCHTIQRITLNGDTLNSEITLIGGNDKGSFVSSVVSGSLAEKAGLREGHQLLLLEGCIKGENQSVPLDTCTKEEVHWTIQRCHGPVTLQYKSNHEGYRKLLSELEDGLIASGDSFHIRLNLNISSQLDCCSLSVKCDEIVHILDTMYQGTCGGCDWLCARVDPFTDKDLERGTIPSYSRAQQLLLVKLQRLMHRGSKDETESSHNTLRALRNTLQPEEPAPQSDPKTSPRLSRASFLLGQILQFVSRSENKYKRMNSNERVRIVTGWPSGLARTASEAKKPFPDKLEDLDSKSEINKRLIPYSLVRPIHCERRRPVLFTPTMLAKSLVQKLLNSGGALEFDICKPDIVTKEEFLRRQRMETIIFSREKNLSTYECIVPANIEAVSAKNKHCLLEAGISCTKDLIKAKIYPIVLFIRVSEKNIKRFRKLLPKPEAEDEFLRMCRLKEKELEELPCLYASVEADAWSSIEDLIRTIKDKIGEEQRKTIWIDEDQL A964C1E9DD169D27 map3k7-2 1 TAK1 6 putative self putative self putative self putative self mouse-b-cell Mus musculus B-cell b-cell B-cell [B lymphocyte; B-lymphocyte; B cell; B-cells] phosphorylation phosphorylation reaction phosphorylation true Figure 2A tak1-carma1-2 4 CARMA1 7 unspecified role unspecified role bait bait TAK1 6 unspecified role unspecified role prey prey physical association physical association Figure 4B tak1-carma1-3 2 CARMA1 10 unspecified role unspecified role prey prey TAK1 6 unspecified role unspecified role bait bait required to bind required to bind necessary binding region required to bind western blot western blot Immuno blot Immuno blot certain certain sequence position certain certain certain sequence position certain tak1_flagtag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged western blot western blot Immuno blot Immuno blot undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Mouse TAK1 dB mutant-COOH terminal deleted (aa 531-579) physical association physical association Figure 4A, 5B carma1-malt1-1 3 BCL10 9 unspecified role unspecified role prey prey CARMA1 10 unspecified role unspecified role bait bait MALT1 5 unspecified role unspecified role prey prey association association Figure 6C tak1-ikkb-2 1 TAK1 6 enzyme enzyme unspecified role unspecified role nucl transfection nucleic acid transfection nucl transfection tak1_flagtag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged western blot western blot Immuno blot Immuno blot undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. GST-IKKB 8 enzyme target enzyme target substrate substrate unspecified role unspecified role nucl transfection nucleic acid transfection nucl transfection ikkb_gsttag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag western blot western blot Immuno blot Immuno blot undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O14920:p.[Ser177Ala;Thr180Ala] mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain T A Wed May 02 19:32:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser177ala,thr180ala' Tue Jun 30 17:57:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser177Ala;Thr180Ala]' human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. phosphorylation phosphorylation reaction phosphorylation Figure 5D GST-IKKB which is S177 and S181 mutated to A177 and A181 shows no phosphorylation.