IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Structural basis of ligand recognition by PABC, a highly specific peptide-binding domain found in poly(A)-binding protein and a HECT ubiquitin ligase. Structural basis of ligand recognition by PABC, a highly specific peptide-binding domain found in poly(A)-binding protein and a HECT ubiquitin ligase. EMBO J. (0261-4189) 2004 imex curation Kozlov G., De Crescenzo G., Lim N.S., Siddiqui N., Fantus D., Kahvejian A., Trempe J.F., Elias D., Ekiel I., Sonenberg N., O'Connor-McCourt M., Gehring K. kalle.gehring@mcgill.ca 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Kozlov G., De Crescenzo G., Lim N.S., Siddiqui N., Fantus D., Kahvejian A., Trempe J.F., Elias D., Ekiel I., Sonenberg N., O'Connor-McCourt M., Gehring K. kalle.gehring@mcgill.ca Accepted 2013-NOV-05 by ORCHARD 22-Nov-2013: Contacted by IntAct-Help. EMBO J. (0261-4189) 2004 Only protein-protein interactions imex curation Structural basis of ligand recognition by PABC, a highly specific peptide-binding domain found in poly(A)-binding protein and a HECT ubiquitin ligase. Structural basis of ligand recognition by PABC, a highly specific peptide-binding domain found in poly(A)-binding protein and a HECT ubiquitin ligase. EMBO J. (0261-4189) 2004 imex curation Kozlov G., De Crescenzo G., Lim N.S., Siddiqui N., Fantus D., Kahvejian A., Trempe J.F., Elias D., Ekiel I., Sonenberg N., O'Connor-McCourt M., Gehring K. kalle.gehring@mcgill.ca 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Kozlov G., De Crescenzo G., Lim N.S., Siddiqui N., Fantus D., Kahvejian A., Trempe J.F., Elias D., Ekiel I., Sonenberg N., O'Connor-McCourt M., Gehring K. kalle.gehring@mcgill.ca Accepted 2013-NOV-05 by ORCHARD 22-Nov-2013: Contacted by IntAct-Help. EMBO J. (0261-4189) 2004 Only protein-protein interactions imex curation Structural basis of ligand recognition by PABC, a highly specific peptide-binding domain found in poly(A)-binding protein and a HECT ubiquitin ligase. Structural basis of ligand recognition by PABC, a highly specific peptide-binding domain found in poly(A)-binding protein and a HECT ubiquitin ligase. EMBO J. (0261-4189) 2004 imex curation Kozlov G., De Crescenzo G., Lim N.S., Siddiqui N., Fantus D., Kahvejian A., Trempe J.F., Elias D., Ekiel I., Sonenberg N., O'Connor-McCourt M., Gehring K. kalle.gehring@mcgill.ca 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Kozlov G., De Crescenzo G., Lim N.S., Siddiqui N., Fantus D., Kahvejian A., Trempe J.F., Elias D., Ekiel I., Sonenberg N., O'Connor-McCourt M., Gehring K. kalle.gehring@mcgill.ca Accepted 2013-NOV-05 by ORCHARD 22-Nov-2013: Contacted by IntAct-Help. EMBO J. (0261-4189) 2004 Only protein-protein interactions imex curation Structural basis of ligand recognition by PABC, a highly specific peptide-binding domain found in poly(A)-binding protein and a HECT ubiquitin ligase. Structural basis of ligand recognition by PABC, a highly specific peptide-binding domain found in poly(A)-binding protein and a HECT ubiquitin ligase. EMBO J. (0261-4189) 2004 imex curation Kozlov G., De Crescenzo G., Lim N.S., Siddiqui N., Fantus D., Kahvejian A., Trempe J.F., Elias D., Ekiel I., Sonenberg N., O'Connor-McCourt M., Gehring K. kalle.gehring@mcgill.ca 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Kozlov G., De Crescenzo G., Lim N.S., Siddiqui N., Fantus D., Kahvejian A., Trempe J.F., Elias D., Ekiel I., Sonenberg N., O'Connor-McCourt M., Gehring K. kalle.gehring@mcgill.ca Accepted 2013-NOV-05 by ORCHARD 22-Nov-2013: Contacted by IntAct-Help. EMBO J. (0261-4189) 2004 Only protein-protein interactions imex curation paip1_human Polyadenylate-binding protein-interacting protein 1 PAIP1 protein protein human Homo sapiens Human MSDGFDRAPGAGRGRSRGLGRGGGGPEGGGFPNGAGPAERARHQPPQPKAPGFLQPPPLRQPRTTPPPGAQCEVPASPQRPSRPGALPEQTRPLRAPPSSQDKIPQQNSESAMAKPQVVVAPVLMSKLSVNAPEFYPSGYSSSYTESYEDGCEDYPTLSEYVQDFLNHLTEQPGSFETEIEQFAETLNGCVTTDDALQELVELIYQQATSIPNFSYMGARLCNYLSHHLTISPQSGNFRQLLLQRCRTEYEVKDQAAKGDEVTRKRFHAFVLFLGELYLNLEIKGTNGQVTRADILQVGLRELLNALFSNPMDDNLICAVKLLKLTGSVLEDAWKEKGKMDMEEIIQRIENVVLDANCSRDVKQMLLKLVELRSSNWGRVHATSTYREATPENDPNYFMNEPTFYTSDGVPFTAADPDYQEKYQELLEREDFFPDYEENGTDLSGAGDPYLDDIDDEMDPEIEEAYEKFCLESERKRKQ A4820607190A3A43 erf3b_human Eukaryotic peptide chain release factor GTP-binding subunit ERF3B GSPT2 ERF3B G1 to S phase transition protein 2 homolog protein protein human Homo sapiens Human MDSGSSSSDSAPDCWDQVDMESPGSAPSGDGVSSAVAEAQREPLSSAFSRKLNVNAKPFVPNVHAAEFVPSFLRGPTQPPTLPAGSGSNDETCTGAGYPQGKRMGRGAPVEPSREEPLVSLEGSNSAVTMELSEPVVENGEVEMALEESWEHSKEVSEAEPGGGSSGDSGPPEESGQEMMEEKEEIRKSKSVIVPSGAPKKEHVNVVFIGHVDAGKSTIGGQIMFLTGMVDKRTLEKYEREAKEKNRETWYLSWALDTNQEERDKGKTVEVGRAYFETERKHFTILDAPGHKSFVPNMIGGASQADLAVLVISARKGEFETGFEKGGQTREHAMLAKTAGVKHLIVLINKMDDPTVNWSIERYEECKEKLVPFLKKVGFSPKKDIHFMPCSGLTGANIKEQSDFCPWYTGLPFIPYLDNLPNFNRSIDGPIRLPIVDKYKDMGTVVLGKLESGSIFKGQQLVMMPNKHNVEVLGILSDDTETDFVAPGENLKIRLKGIEEEEILPGFILCDPSNLCHSGRTFDVQIVIIEHKSIICPGYNAVLHIHTCIEEVEITALISLVDKKSGEKSKTRPRFVKQDQVCIARLRTAGTICLETFKDFPQMGRFTLRDEGKTIAIGKVLKLVPEKD paip2_human Polyadenylate-binding protein-interacting protein 2 PAIP2 PAIP2A HSPC218 protein protein human Homo sapiens Human MKDPSRSSTSPSIINEDVIINGHSHEDDNPFAEYMWMENEEEFNRQIEEELWEEEFIERCFQEMLEEEEEHEWFIPARDLPQTMDQIQDQFNDLVISDGSSLEDLVVKSNLNPNAKEFVPGVKYGNI CAC27C537E5C6E22 pabp1_human Polyadenylate-binding protein 1 PABPC1 PAB1 PABP1 PABPC2 PABP protein protein human Homo sapiens Human MNPSAPSYPMASLYVGDLHPDVTEAMLYEKFSPAGPILSIRVCRDMITRRSLGYAYVNFQQPADAERALDTMNFDVIKGKPVRIMWSQRDPSLRKSGVGNIFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDENGSKGYGFVHFETQEAAERAIEKMNGMLLNDRKVFVGRFKSRKEREAELGARAKEFTNVYIKNFGEDMDDERLKDLFGKFGPALSVKVMTDESGKSKGFGFVSFERHEDAQKAVDEMNGKELNGKQIYVGRAQKKVERQTELKRKFEQMKQDRITRYQGVNLYVKNLDDGIDDERLRKEFSPFGTITSAKVMMEGGRSKGFGFVCFSSPEEATKAVTEMNGRIVATKPLYVALAQRKEERQAHLTNQYMQRMASVRAVPNPVINPYQPAPPSGYFMAAIPQTQNRAAYYPPSQIAQLRPSPRWTAQGARPHPFQNMPGAIRPAAPRPPFSTMRPASSQVPRVMSTQRVANTSTQTMGPRPAAAAAAATPAVRTVPQYKYAAGVRNPQQHLNAQPQVTMQQPAVHVQGQEPLTASMLASAPPQEQKQMLGERLFPLIQAMHPTLAGKITGMLLEIDNSELLHMLESPESLRSKVDEAVAVLQAHQAKEAAQKAVNSATGVPTV DIP protein P11940 original sequence version: 187 DIP protein P11940 original sequence version: 181 DIP protein P11940-1 original sequence version: 136 DIP protein P11940 original sequence version: 148 DIP protein P11940 original sequence version: 172 2EB1B02A346132EE pabpc1-paip2 3 7 unspecified role unspecified role neutral component neutral component lig_pam2_1 motif sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecobd Escherichia coli (strain B / BL21-DE3) 8 unspecified role unspecified role neutral component neutral component pabp_hyd domain sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecobd Escherichia coli (strain B / BL21-DE3) 14 11 direct interaction direct interaction Fig. 1b, Fig. 1c, Fig. 2a, Fig. 2c, Fig. 3b, Fig. 3c pabpc1-paip1-4 3 8 unspecified role unspecified role neutral component neutral component pabp_hyd domain sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecobd Escherichia coli (strain B / BL21-DE3) 5 unspecified role unspecified role neutral component neutral component region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined lig_pam2_1 motif sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecobd Escherichia coli (strain B / BL21-DE3) 17 20 direct interaction direct interaction Fig 1d, Fig. 2b, Fig. 2d, Fig. 3d pabpc1-paip2-1 1 7 unspecified role unspecified role neutral component neutral component lig_pam2_1 motif sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecobd Escherichia coli (strain B / BL21-DE3) 8 unspecified role unspecified role neutral component neutral component pabp_hyd domain sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecobd Escherichia coli (strain B / BL21-DE3) 23 25 direct interaction direct interaction Fig. 5 Temp = 25C Kd values (molar) were measured at different temperatures: 10C: 7.9 x 10E-8; 15C: 1.4 x 10E-7; 20C: 2.1 x 10E-7; 25C: 3.5 x 10E-7; 30C: 5.6 x 10E-7. pabpc1-paip1-5 1 8 unspecified role unspecified role neutral component neutral component pabp_hyd domain sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecobd Escherichia coli (strain B / BL21-DE3) 5 unspecified role unspecified role neutral component neutral component lig_pam2_1 motif sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecobd Escherichia coli (strain B / BL21-DE3) 28 30 direct interaction direct interaction Fig. 5b, Fig. 5c Temp = 25C Kd values (molar) were measured at different temperatures: 10C: 8.2 x 10E-7; 15C: 9.4 x 10E-7; 20C: 1.3 x 10E-6; 25C: 1.5 x 10E-6. gspt2-pabpc1 1 6 unspecified role unspecified role neutral component neutral component lig_pam2_1 motif sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecobd Escherichia coli (strain B / BL21-DE3) 8 unspecified role unspecified role neutral component neutral component pabp_hyd domain sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecobd Escherichia coli (strain B / BL21-DE3) 35 33 direct interaction direct interaction Fig. 5b, Fig. 5c Temp = 25C Kd values (molar) were measured at different temperatures: 10C: 3.9 x 10E-7; 15C: 7.3 x 10E-7; 20C: 9.2 x 10E-7; 25C: 1.6 x 10E-6; 30C: 2.4 x 10E-6. pabpc1-paip1-6 2 8 unspecified role unspecified role bait bait pabp_hyd domain sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecobd Escherichia coli (strain B / BL21-DE3) 5 unspecified role unspecified role prey prey lig_pam2_1 motif sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecobd Escherichia coli (strain B / BL21-DE3) 38 40 direct interaction direct interaction Fig. 6 Temp = 25C pabpc1-gspt2 2 6 unspecified role unspecified role prey prey lig_pam2_1 motif sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecobd Escherichia coli (strain B / BL21-DE3) 8 unspecified role unspecified role bait bait pabp_hyd domain sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecobd Escherichia coli (strain B / BL21-DE3) 45 43 direct interaction direct interaction Fig. 6c Temp = 25C Kd (molar) for shorter eRF3 peptides: residues 47-65: 3.9 x 10E-6; residues 56-74: 3.1 x 10E-6. pabpc1-paip2-2 2 8 unspecified role unspecified role bait bait pabp_hyd domain sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecobd Escherichia coli (strain B / BL21-DE3) 7 unspecified role unspecified role prey prey Q9BPZ3:p.Leu111Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L A Wed May 02 15:22:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu111ala' Tue Jun 30 19:18:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu111Ala' Q9BPZ3:p.Pro113Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain P A Wed May 02 17:12:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro113ala' Tue Jun 30 18:21:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro113Ala' Q9BPZ3:p.Ala115Leu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain A L Wed May 02 15:00:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala115leu' Tue Jun 30 17:24:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala115Leu' Q9BPZ3:p.Lys116Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K A Wed May 02 16:58:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys116ala' Tue Jun 30 20:03:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys116Ala' Q9BPZ3:p.Glu117Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E A Wed May 02 16:01:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu117ala' Tue Jun 30 16:33:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu117Ala' Q9BPZ3:p.Phe118Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F A Wed May 02 18:53:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe118ala' Tue Jun 30 18:54:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe118Ala' Q9BPZ3:p.Pro120Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain P A Wed May 02 18:50:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro120ala' Tue Jun 30 20:16:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro120Ala' lig_pam2_1 motif sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecobd Escherichia coli (strain B / BL21-DE3) 48 57 direct interaction direct interaction Fig. 6c Temp = 25C Kd values (molar) were measured for peptides of different length: residues 106-127: 1.6 x 10E-7; residues 105-120: 3.0 x 10E-6; residues 105-118: 2.6 x 10E-4; residues 109-125: 1.9 x 10E-7. Kd values (molar) were measured for different mutants of the 109-125 peptide: leu111ala: 2.3 x 10E-4; pro113ala: 7.4 x 10E-7; ala115leu: 1.4 x 10E-5; lys116ala: 4.8 x 10E-7; glu117ala: 3.5 x 10E-7; phe118ala: >10E-3; pro120ala: 3.9 x 10E-6. pabpc1-paip1-7 4 5 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecobd Escherichia coli (strain B / BL21-DE3) 8 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined pabp_hyd domain sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain P11940:p.Phe567Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F A Wed May 02 20:15:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe567ala' Tue Jun 30 16:40:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe567Ala' P11940:p.Lys580Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K A Wed May 02 16:06:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys580ala' Tue Jun 30 17:35:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys580Ala' ecobd Escherichia coli (strain B / BL21-DE3) direct interaction direct interaction Fig. 4a pabpc1-paip2-3 4 7 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecobd Escherichia coli (strain B / BL21-DE3) 8 unspecified role unspecified role bait bait pabp_hyd domain sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P11940:p.Phe567Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F A Wed May 02 20:29:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe567ala' Tue Jun 30 15:42:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe567Ala' P11940:p.Lys580Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K A Wed May 02 16:36:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys580ala' Tue Jun 30 16:19:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys580Ala' ecobd Escherichia coli (strain B / BL21-DE3) direct interaction direct interaction Fig. 4b pabpc1-gspt2-1 4 6 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecobd Escherichia coli (strain B / BL21-DE3) 8 unspecified role unspecified role bait bait pabp_hyd domain sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P11940:p.Phe567Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F A Wed May 02 15:35:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe567ala' Tue Jun 30 16:09:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe567Ala' ecobd Escherichia coli (strain B / BL21-DE3) direct interaction direct interaction Fig. 4c