mbinfo MBInfo http://www.mechanobio.info/ Complex formation with focal adhesion kinase: A mechanism to regulate activity and subcellular localization of Src kinases. Complex formation with focal adhesion kinase: A mechanism to regulate activity and subcellular localization of Src kinases. Mol. Biol. Cell (1059-1524) 1999 imex curation Schaller M.D., Hildebrand J.D., Parsons J.T. Fyn Co-IPs from Fig 6B could not be curated since the source of Fyn could not be identified. crispy4@med.unc.edu 09-Sep-2013: Contacted by IntAct-Help. Only protein-protein interactions chick-embryo Gallus gallus chick embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti bait coip anti bait coimmunoprecipitation anti bait coip autoradiography autoradiography Schaller M.D., Hildebrand J.D., Parsons J.T. Interaction detection: The Src band blotted on to the nitrocellulose was excised, incubated with CNBr and the cleavage products visualized by autoradiography. Fyn Co-IPs from Fig 6B could not be curated since the source of Fyn could not be identified. crispy4@med.unc.edu Accepted 2013-AUG-15 by PPORRAS 09-Sep-2013: Contacted by IntAct-Help. Mol. Biol. Cell (1059-1524) 1999 Only protein-protein interactions imex curation Complex formation with focal adhesion kinase: A mechanism to regulate activity and subcellular localization of Src kinases. Complex formation with focal adhesion kinase: A mechanism to regulate activity and subcellular localization of Src kinases. Mol. Biol. Cell (1059-1524) 1999 imex curation Schaller M.D., Hildebrand J.D., Parsons J.T. Fyn Co-IPs from Fig 6B could not be curated since the source of Fyn could not be identified. crispy4@med.unc.edu 09-Sep-2013: Contacted by IntAct-Help. Only protein-protein interactions chick-embryo Gallus gallus chick embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Schaller M.D., Hildebrand J.D., Parsons J.T. Fyn Co-IPs from Fig 6B could not be curated since the source of Fyn could not be identified. Fyn Co-IPs from Fig 6B could not be curated since the source of Fyn could not be identified. crispy4@med.unc.edu Accepted 2013-AUG-15 by PPORRAS 09-Sep-2013: Contacted by IntAct-Help. Mol. Biol. Cell (1059-1524) 1999 Only protein-protein interactions imex curation fak1_chick Focal adhesion kinase 1 PTK2 FAK FAK1 pp125FAK Protein-tyrosine kinase 2 Focal adhesion kinase-related nonkinase p125FAK protein protein chick Gallus gallus (Chicken) Chicken MAAAYLDPNLNHTPSSSAKTHLGTGMERSPGAMERVLKVFHYFENSSEPTTWASIIRHGDATDVRGIIQKIVDCHKVKNVACYGLRLSHLQSEEVHWLHLDMGVSNVREKFELAHPPEEWKYELRIRYLPKGFLNQFTEDKPTLNFFYQQVKNDYMLEIADQVDQEIALKLGCLEIRRSYGEMRGNALEKKSNYEVLEKDVGLRRFFPKSLLDSVKAKTLRKLIQQTFRQFANLNREESILKFFEILSPVYRFDKECFKCALGSSWIISVELAIGPEEGISYLTDKGANPTHLADFNQVQTIQYSNSEDKDRKGMLQLKIAGAPEPLTVTAPSLTIAENMADLIDGYCRLVNGATQSFIIRPQKEGERALPSIPKLANNEKQGVRSHTVSVSETDDYAEIIDEEDTYTMPSTRDYEIQRERIELGRCIGEGQFGDVHQGIYMSPENPAMAVAIKTCKNCTSDSVREKFLQEALTMRQFDHPHIVKLIGVITENPVWIIMELCTLGELRSFLQVRKFSLDLASLILYAYQLSTALAYLESKRFVHRDIAARNVLVSATDCVKLGDFGLSRYMEDSTYYKASKGKLPIKWMAPESINFRRFTSASDVWMFGVCMWEILMHGVKPFQGVKNNDVIGRIENGERLPMPPNCPPTLYSLMTKCWAYDPSRRPRFTELKAQLSTILEEEKLQQEERMRMESRRQVTVSWDSGGSDEAPPKPSRPGYPSPRSSEGFYPSPQHMVQPNHYQVSGYSGSHGIPAMAGSIYPGQASLLDQTDSWNHRPQEVSAWQPNMEDSGTLDVRGMGQVLPTHLMEERLIRQQQEMEEDQRWLEKEERFLVMKPDVRLSRGSIEREDGGLQGPAGNQHIYQPVGKPDHAAPPKKPPRPGAPHLGSLASLNSPVDSYNEGVKIKPQEISPPPTANLDRSNDKVYENVTGLVKAVIEMSSKIQPAPPEEYVPMVKEVGLALRTLLATVDESLPVLPASTHREIEMAQKLLNSDLAELINKMKLAQQYVMTSLQQEYKKQMLTAAHALAVDAKNLLDVIDQARLKMISQSRPH 8051154219B953B9 src_chick Proto-oncogene tyrosine-protein kinase Src SRC pp60c-src Proto-oncogene c-Src protein protein chick Gallus gallus (Chicken) Chicken MGSSKSKPKDPSQRRRSLEPPDSTHHGGFPASQTPNKTAAPDTHRTPSRSFGTVATEPKLFGGFNTSDTVTSPQRAGALAGGVTTFVALYDYESRTETDLSFKKGERLQIVNNTEGDWWLAHSLTTGQTGYIPSNYVAPSDSIQAEEWYFGKITRRESERLLLNPENPRGTFLVRESETTKGAYCLSVSDFDNAKGLNVKHYKIRKLDSGGFYITSRTQFSSLQQLVAYYSKHADGLCHRLTNVCPTSKPQTQGLAKDAWEIPRESLRLEVKLGQGCFGEVWMGTWNGTTRVAIKTLKPGTMSPEAFLQEAQVMKKLRHEKLVQLYAVVSEEPIYIVTEYMSKGSLLDFLKGEMGKYLRLPQLVDMAAQIASGMAYVERMNYVHRDLRAANILVGENLVCKVADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELTTKGRVPYPGMVNREVLDQVERGYRMPCPPECPESLHDLMCQCWRKDPEERPTFEYLQAFLEDYFTSTEPQYQPGENL DIP protein P00523 original sequence version: 105 ABDB036F7D63C30A src-ptk2 2 3 unspecified role unspecified role prey prey Q00944:p.[Tyr576Phe;Tyr577Phe] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y F certain certain sequence position certain certain certain sequence position certain Y F Fri Jul 22 11:12:59 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr577phe,tyr576phe' Wed May 02 16:29:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr576phe,tyr577phe' Tue Jun 30 16:30:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr576Phe;Tyr577Phe]' Q00944:p.Tyr397Phe mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 18:16:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr397phe' Tue Jun 30 19:22:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr397Phe' Q00944:p.[Tyr397Phe;Lys454Arg] mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y F certain certain sequence position certain certain certain sequence position certain K R Wed May 02 19:02:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr397phe,lys454arg' Tue Jun 30 19:30:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr397Phe;Lys454Arg]' Q00944:p.Lys454Arg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K R Wed May 02 17:13:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys454arg' Tue Jun 30 16:15:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys454Arg' 4 unspecified role unspecified role bait bait physical association physical association Fig 6A. Src coimmunoprecipitated exogenously expressed FAK as well as endogenous FAK (though to a lesser extent). ptk2-src-1 1 3 unspecified role unspecified role bait bait orthophosphate 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined FAK was labelled with 32p-orthophosphate. 4 unspecified role unspecified role prey prey orthophosphate 32p radiolabel 32p radiolabel P32 32P 32P P32 autoradiography autoradiography undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 9A, 9B. CNBr cleavage of the coimmunoprecipitated 60 KDa Src band yielded two fragments of 30 and 8-9 KDa. The 4 KDa fragment containing the Tyr527 wasmissing indicating a change in Src conformation upon binding to FAK.