mbinfo MBInfo http://www.mechanobio.info/ WAVE, a novel WASP-family protein involved in actin reorganization induced by Rac. WAVE, a novel WASP-family protein involved in actin reorganization induced by Rac. EMBO J. (0261-4189) 1998 imex curation Miki H., Suetsugu S., Takenawa T. Interaction detection: GST pull down. Interactions involving Rac1 and CDC42 could not be curated as the species of origin of the constructs could not be traced. takenawa@ims.u-tokyo.ac.jp 11-Oct-2012: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Miki H., Suetsugu S., Takenawa T. Interaction detection: GST pull down. Interactions involving Rac1 and CDC42 could not be curated as the species of origin of the constructs could not be traced. takenawa@ims.u-tokyo.ac.jp Accepted 2012-SEP-06 by ORCHARD 11-Oct-2012: Contacted by IntAct-Help. EMBO J. (0261-4189) 1998 Only protein-protein interactions imex curation WAVE, a novel WASP-family protein involved in actin reorganization induced by Rac. WAVE, a novel WASP-family protein involved in actin reorganization induced by Rac. EMBO J. (0261-4189) 1998 imex curation Miki H., Suetsugu S., Takenawa T. Interaction detection: GST pull down. Interactions involving Rac1 and CDC42 could not be curated as the species of origin of the constructs could not be traced. takenawa@ims.u-tokyo.ac.jp 11-Oct-2012: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting western blot western blot Immuno blot Immuno blot Miki H., Suetsugu S., Takenawa T. Interaction detection: GST pull down. Interactions involving Rac1 and CDC42 could not be curated as the species of origin of the constructs could not be traced. takenawa@ims.u-tokyo.ac.jp Accepted 2012-SEP-06 by ORCHARD 11-Oct-2012: Contacted by IntAct-Help. EMBO J. (0261-4189) 1998 Only protein-protein interactions imex curation WAVE, a novel WASP-family protein involved in actin reorganization induced by Rac. WAVE, a novel WASP-family protein involved in actin reorganization induced by Rac. EMBO J. (0261-4189) 1998 imex curation Miki H., Suetsugu S., Takenawa T. Interaction detection: GST pull down. Interactions involving Rac1 and CDC42 could not be curated as the species of origin of the constructs could not be traced. takenawa@ims.u-tokyo.ac.jp 11-Oct-2012: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Miki H., Suetsugu S., Takenawa T. Interaction detection: GST pull down. Interactions involving Rac1 and CDC42 could not be curated as the species of origin of the constructs could not be traced. takenawa@ims.u-tokyo.ac.jp Accepted 2012-SEP-06 by ORCHARD 11-Oct-2012: Contacted by IntAct-Help. EMBO J. (0261-4189) 1998 Only protein-protein interactions imex curation wasf1_human Wiskott-Aldrich syndrome protein family member 1 WASF1 KIAA0269 SCAR1 WAVE1 Protein WAVE-1 Verprolin homology domain-containing protein 1 protein protein human Homo sapiens Human MPLVKRNIDPRHLCHTALPRGIKNELECVTNISLANIIRQLSSLSKYAEDIFGELFNEAHSFSFRVNSLQERVDRLSVSVTQLDPKEEELSLQDITMRKAFRSSTIQDQQLFDRKTLPIPLQETYDVCEQPPPLNILTPYRDDGKEGLKFYTNPSYFFDLWKEKMLQDTEDKRKEKRKQKQKNLDRPHEPEKVPRAPHDRRREWQKLAQGPELAEDDANLLHKHIEVANGPASHFETRPQTYVDHMDGSYSLSALPFSQMSELLTRAEERVLVRPHEPPPPPPMHGAGDAKPIPTCISSATGLIENRPQSPATGRTPVFVSPTPPPPPPPLPSALSTSSLRASMTSTPPPPVPPPPPPPATALQAPAVPPPPAPLQIAPGVLHPAPPPIAPPLVQPSPPVARAAPVCETVPVHPLPQGEVQGLPPPPPPPPLPPPGIRPSSPVTVTALAHPPSGLHPTPSTAPGPHVPLMPPSPPSQVIPASEPKRHPSTLPVISDARSVLLEAIRKGIQLRKVEEQREQEAKHERIENDVATILSRRIAVEYSDSEDDSEFDEVDWLE DIP protein Q92558 original sequence version: 78 DIP protein Q92558 original sequence version: 82 DIP protein Q92558 original sequence version: 124 DIP protein Q92558 original sequence version: 87 44B4527BDB77BC6E prof1_human Profilin-1 Profilin I PFN1 Epididymis tissue protein Li 184a protein protein human Homo sapiens Human MAGWNAYIDNLMADGTCQDAAIVGYKDSPSVWAAVPGKTFVNITPAEVGVLVGKDRSSFYVNGLTLGGQKCSVIRDSLLQDGEFSMDLRTKSTGGAPTFNVTVTKTDKTLVLLMGKEGVHGGLINKKCYEMASHLRRSQY DIP protein P07737 original sequence version: 73 DIP protein P07737 original sequence version: 160 DIP protein P07737 original sequence version: 97 DIP protein P07737 original sequence version: 98 DIP protein P07737 original sequence version: 168 DIP protein P07737 original sequence version: 140 F725119E55A289EB prof2_human Profilin-2 PFN2 Profilin II protein protein human Homo sapiens Human MAGWQSYVDNLMCDGCCQEAAIVGYCDAKYVWAATAGGVFQSITPIEIDMIVGKDREGFFTNGLTLGAKKCSVIRDSLYVDGDCTMDIRTKSQGGEPTYNVAVGRAGRVLVFVMGKEGVHGGGLNKKAYSMAKYLRDSGF F43884E1427C9A99 acts_chick Actin, alpha skeletal muscle ACTA1 ACTA Alpha-actin-1 protein protein chick Gallus gallus (Chicken) Chicken MCDEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKCF DIP protein P68139 original sequence version: 86 DF2A3A046346A179 pfn1-wasf1-2 1 5 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role prey prey proline-rich region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain vph region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 physical association physical association Fig 3A (lane 3), 3D, 6D. pfn2-wasf1-1 1 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 physical association physical association Fig 3A (lane 4). pfn1-wasf1-4 2 5 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role prey prey chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 physical association physical association Fig 3B. wasf1-acta1-3 3 7 unspecified role unspecified role prey prey chick Gallus gallus (Chicken) Chicken 4 unspecified role unspecified role bait bait vph region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain c-terminal region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Fig 6A, 6C.