mbinfo MBInfo http://www.mechanobio.info/ Filamin binds to the cytoplasmic domain of the beta1-integrin. Identification of amino acids responsible for this interaction. Filamin binds to the cytoplasmic domain of the beta1-integrin. Identification of amino acids responsible for this interaction. J. Biol. Chem. (0021-9258) 1998 imex curation Loo D.T., Kanner S.B., Aruffo A. The source of filamin inferred from the cDNA library used. The source of integrin beta-1 inferred from the protein sequence length. lood@bms.com Only protein-protein interactions in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa tag perox activity tag visualisation by peroxidase activity tag perox activity Loo D.T., Kanner S.B., Aruffo A. Interaction between integrin beta-1 and alpha-actinin also shown by ELISA, but could not be curated because of information missing on the source (organism) of alpha-actinin used. The source of filamin inferred from the cDNA library used. The source of integrin beta-1 inferred from the protein sequence length. lood@bms.com Accepted 2012-MAR-07 by ORCHARD J. Biol. Chem. (0021-9258) 1998 Only protein-protein interactions imex curation Filamin binds to the cytoplasmic domain of the beta1-integrin. Identification of amino acids responsible for this interaction. Filamin binds to the cytoplasmic domain of the beta1-integrin. Identification of amino acids responsible for this interaction. J. Biol. Chem. (0021-9258) 1998 imex curation Loo D.T., Kanner S.B., Aruffo A. The source of filamin inferred from the cDNA library used. The source of integrin beta-1 inferred from the protein sequence length. lood@bms.com Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Loo D.T., Kanner S.B., Aruffo A. The source of filamin inferred from the cDNA library used. The source of integrin beta-1 inferred from the protein sequence length. lood@bms.com Accepted 2012-MAR-07 by ORCHARD J. Biol. Chem. (0021-9258) 1998 Only protein-protein interactions imex curation Filamin binds to the cytoplasmic domain of the beta1-integrin. Identification of amino acids responsible for this interaction. Filamin binds to the cytoplasmic domain of the beta1-integrin. Identification of amino acids responsible for this interaction. J. Biol. Chem. (0021-9258) 1998 imex curation Loo D.T., Kanner S.B., Aruffo A. The source of filamin inferred from the cDNA library used. The source of integrin beta-1 inferred from the protein sequence length. lood@bms.com Only protein-protein interactions human-jurkat Homo sapiens jurkat Human T cell leukemia anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Loo D.T., Kanner S.B., Aruffo A. The source of filamin inferred from the cDNA library used. The source of integrin beta-1 inferred from the protein sequence length. lood@bms.com Accepted 2012-MAR-07 by ORCHARD J. Biol. Chem. (0021-9258) 1998 Only protein-protein interactions imex curation itb1_human Integrin beta-1 Fibronectin receptor subunit beta VLA-4 subunit beta ITGB1 FNRB MDF2 MSK12 Glycoprotein IIa protein protein human Homo sapiens Human MNLQPIFWIGLISSVCCVFAQTDENRCLKANAKSCGECIQAGPNCGWCTNSTFLQEGMPTSARCDDLEALKKKGCPPDDIENPRGSKDIKKNKNVTNRSKGTAEKLKPEDITQIQPQQLVLRLRSGEPQTFTLKFKRAEDYPIDLYYLMDLSYSMKDDLENVKSLGTDLMNEMRRITSDFRIGFGSFVEKTVMPYISTTPAKLRNPCTSEQNCTSPFSYKNVLSLTNKGEVFNELVGKQRISGNLDSPEGGFDAIMQVAVCGSLIGWRNVTRLLVFSTDAGFHFAGDGKLGGIVLPNDGQCHLENNMYTMSHYYDYPSIAHLVQKLSENNIQTIFAVTEEFQPVYKELKNLIPKSAVGTLSANSSNVIQLIIDAYNSLSSEVILENGKLSEGVTISYKSYCKNGVNGTGENGRKCSNISIGDEVQFEISITSNKCPKKDSDSFKIRPLGFTEEVEVILQYICECECQSEGIPESPKCHEGNGTFECGACRCNEGRVGRHCECSTDEVNSEDMDAYCRKENSSEICSNNGECVCGQCVCRKRDNTNEIYSGKFCECDNFNCDRSNGLICGGNGVCKCRVCECNPNYTGSACDCSLDTSTCEASNGQICNGRGICECGVCKCTDPKFQGQTCEMCQTCLGVCAEHKECVQCRAFNKGEKKDTCTQECSYFNITKVESRDKLPQPVQPDPVSHCKEKDVDDCWFYFTYSVNGNNEVMVHVVENPECPTGPDIIPIVAGVVAGIVLIGLALLLIWKLLMIIHDRREFAKFEKEKMNAKWDTGENPIYKSAVTTVVNPKYEGK DIP protein P05556 original sequence version: 139 DIP protein P05556-1 original sequence version: 143 DIP protein P05556 original sequence version: 154 DIP protein P05556 original sequence version: 167 DE35979C1625578C itb1_chick Integrin beta-1 ITGB1 CSAT antigen JG22 antigen RGD-receptor protein protein chick Gallus gallus (Chicken) Chicken MAETNLTLLTWAGILCCLIWSGSAQQGGSDCIKANAKSCGECIQAGPNCGWCKKTDFLQEGEPTSARCDDLAALKSKGCPEQDIENPRGSKRVLEDREVTNRKIGAAEKLKPEAITQIQPQKLVLQLRVGEPQTFSLKFKRAEDYPIDLYYLMDLSYSMKDDLENVKSLGTALMREMEKITSDFRIGFGSFVEKTVMPYISTTPAKLRNPCTGDQNCTSPFSYKNVLSLTSEGNKFNELVGKQHISGNLDSPEGGFDAIMQVAVCGDQIGWRNVTRLLVFSTDAGFHFAGDGKLGGIVLPNDGKCHLENNMYTMSHYYDYPSIAHLVQKLSENNIQTIFAVTEEFQAVYKELKNLIPKSAVGTLSSNSSNVIQLIIDAYNSLSSEVILENSKLPKEVTISYKSYCKNGVNDTQEDGRKCSNISIGDEVRFEINVTANECPKKGQNETIKIKPLGFTEEVEIHLQFICDCLCQSEGEPNSPACHDGNGTFECGACRCNEGRIGRLCECSTDEVNSEDMDAYCRRENSTEICSNNGECICGQCVCKKRENTNEVYSGKYCECDNFNCDRSNGLICGGNGICKCRVCECFPNFTGSACDCSLDTTPCMAGNGQICNGRGTCECGTCNCTDPKFQGPTCEMCQTCLGVCAEHKDCVQCRAFEKGEKKETCSQECMHFNMTRVESRGKLPQPVHPDPLSHCKEKDVGDCWFYFTYSVNSNGEASVHVVETPECPSGPDIIPIVAGVVAGIVLIGLALLLIWKLLMIIHDRREFAKFEKEKMNAKWDTGENPIYKSAVTTVVNPKYEGK 2F6FEFCDF2C80457 flna_human Filamin-A FLNA FLN FLN1 Alpha-filamin Filamin-1 Endothelial actin-binding protein Actin-binding protein 280 Non-muscle filamin protein protein human Homo sapiens Human MSSSHSRAGQSAAGAAPGGGVDTRDAEMPATEKDLAEDAPWKKIQQNTFTRWCNEHLKCVSKRIANLQTDLSDGLRLIALLEVLSQKKMHRKHNQRPTFRQMQLENVSVALEFLDRESIKLVSIDSKAIVDGNLKLILGLIWTLILHYSISMPMWDEEEDEEAKKQTPKQRLLGWIQNKLPQLPITNFSRDWQSGRALGALVDSCAPGLCPDWDSWDASKPVTNAREAMQQADDWLGIPQVITPEEIVDPNVDEHSVMTYLSQFPKAKLKPGAPLRPKLNPKKARAYGPGIEPTGNMVKKRAEFTVETRSAGQGEVLVYVEDPAGHQEEAKVTANNDKNRTFSVWYVPEVTGTHKVTVLFAGQHIAKSPFEVYVDKSQGDASKVTAQGPGLEPSGNIANKTTYFEIFTAGAGTGEVEVVIQDPMGQKGTVEPQLEARGDSTYRCSYQPTMEGVHTVHVTFAGVPIPRSPYTVTVGQACNPSACRAVGRGLQPKGVRVKETADFKVYTKGAGSGELKVTVKGPKGEERVKQKDLGDGVYGFEYYPMVPGTYIVTITWGGQNIGRSPFEVKVGTECGNQKVRAWGPGLEGGVVGKSADFVVEAIGDDVGTLGFSVEGPSQAKIECDDKGDGSCDVRYWPQEAGEYAVHVLCNSEDIRLSPFMADIRDAPQDFHPDRVKARGPGLEKTGVAVNKPAEFTVDAKHGGKAPLRVQVQDNEGCPVEALVKDNGNGTYSCSYVPRKPVKHTAMVSWGGVSIPNSPFRVNVGAGSHPNKVKVYGPGVAKTGLKAHEPTYFTVDCAEAGQGDVSIGIKCAPGVVGPAEADIDFDIIRNDNDTFTVKYTPRGAGSYTIMVLFADQATPTSPIRVKVEPSHDASKVKAEGPGLSRTGVELGKPTHFTVNAKAAGKGKLDVQFSGLTKGDAVRDVDIIDHHDNTYTVKYTPVQQGPVGVNVTYGGDPIPKSPFSVAVSPSLDLSKIKVSGLGEKVDVGKDQEFTVKSKGAGGQGKVASKIVGPSGAAVPCKVEPGLGADNSVVRFLPREEGPYEVEVTYDGVPVPGSPFPLEAVAPTKPSKVKAFGPGLQGGSAGSPARFTIDTKGAGTGGLGLTVEGPCEAQLECLDNGDGTCSVSYVPTEPGDYNINILFADTHIPGSPFKAHVVPCFDASKVKCSGPGLERATAGEVGQFQVDCSSAGSAELTIEICSEAGLPAEVYIQDHGDGTHTITYIPLCPGAYTVTIKYGGQPVPNFPSKLQVEPAVDTSGVQCYGPGIEGQGVFREATTEFSVDARALTQTGGPHVKARVANPSGNLTETYVQDRGDGMYKVEYTPYEEGLHSVDVTYDGSPVPSSPFQVPVTEGCDPSRVRVHGPGIQSGTTNKPNKFTVETRGAGTGGLGLAVEGPSEAKMSCMDNKDGSCSVEYIPYEAGTYSLNVTYGGHQVPGSPFKVPVHDVTDASKVKCSGPGLSPGMVRANLPQSFQVDTSKAGVAPLQVKVQGPKGLVEPVDVVDNADGTQTVNYVPSREGPYSISVLYGDEEVPRSPFKVKVLPTHDASKVKASGPGLNTTGVPASLPVEFTIDAKDAGEGLLAVQITDPEGKPKKTHIQDNHDGTYTVAYVPDVTGRYTILIKYGGDEIPFSPYRVRAVPTGDASKCTVTVSIGGHGLGAGIGPTIQIGEETVITVDTKAAGKGKVTCTVCTPDGSEVDVDVVENEDGTFDIFYTAPQPGKYVICVRFGGEHVPNSPFQVTALAGDQPSVQPPLRSQQLAPQYTYAQGGQQTWAPERPLVGVNGLDVTSLRPFDLVIPFTIKKGEITGEVRMPSGKVAQPTITDNKDGTVTVRYAPSEAGLHEMDIRYDNMHIPGSPLQFYVDYVNCGHVTAYGPGLTHGVVNKPATFTVNTKDAGEGGLSLAIEGPSKAEISCTDNQDGTCSVSYLPVLPGDYSILVKYNEQHVPGSPFTARVTGDDSMRMSHLKVGSAADIPINISETDLSLLTATVVPPSGREEPCLLKRLRNGHVGISFVPKETGEHLVHVKKNGQHVASSPIPVVISQSEIGDASRVRVSGQGLHEGHTFEPAEFIIDTRDAGYGGLSLSIEGPSKVDINTEDLEDGTCRVTYCPTEPGNYIINIKFADQHVPGSPFSVKVTGEGRVKESITRRRRAPSVANVGSHCDLSLKIPEISIQDMTAQVTSPSGKTHEAEIVEGENHTYCIRFVPAEMGTHTVSVKYKGQHVPGSPFQFTVGPLGEGGAHKVRAGGPGLERAEAGVPAEFSIWTREAGAGGLAIAVEGPSKAEISFEDRKDGSCGVAYVVQEPGDYEVSVKFNEEHIPDSPFVVPVASPSGDARRLTVSSLQESGLKVNQPASFAVSLNGAKGAIDAKVHSPSGALEECYVTEIDQDKYAVRFIPRENGVYLIDVKFNGTHIPGSPFKIRVGEPGHGGDPGLVSAYGAGLEGGVTGNPAEFVVNTSNAGAGALSVTIDGPSKVKMDCQECPEGYRVTYTPMAPGSYLISIKYGGPYHIGGSPFKAKVTGPRLVSNHSLHETSSVFVDSLTKATCAPQHGAPGPGPADASKVVAKGLGLSKAYVGQKSSFTVDCSKAGNNMLLVGVHGPRTPCEEILVKHVGSRLYSVSYLLKDKGEYTLVVKWGDEHIPGSPYRVVVP DIP protein P21333 original sequence version: 185 DIP protein P21333-1 original sequence version: 187 DIP protein P21333 original sequence version: 115 DIP protein P21333 original sequence version: 144 DIP protein P21333-1 original sequence version: 156 DIP protein P21333 original sequence version: 201 DIP protein P21333 original sequence version: 197 DIP protein P21333 original sequence version: 170 6C1A07041DF50142 flna-itgb1-1 2 5 unspecified role unspecified role bait bait c-terminal region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain P07228:p.Asp764Val mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D V Wed May 02 20:09:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp764val' Tue Jun 30 20:22:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp764Val' P07228:p.Tyr788Glu mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y E Wed May 02 15:02:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr788glu' Tue Jun 30 17:18:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr788Glu' P07228:p.Tyr788Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y A Wed May 02 16:27:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr788ala' Tue Jun 30 18:25:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr788Ala' P07228:p.Tyr788Phe mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 15:07:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr788phe' Tue Jun 30 18:40:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr788Phe' P07228:p.Asn797Asp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain N D Wed May 02 19:38:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn797asp' Tue Jun 30 17:58:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn797Asp' P07228:p.Asn785Asp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain N D Wed May 02 18:56:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn785asp' Tue Jun 30 19:10:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn785Asp' P07228:p.Pro786Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain P A Wed May 02 19:45:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro786ala' Tue Jun 30 18:26:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro786Ala' P07228:p.Asn797Ile mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain N I Wed May 02 14:25:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn797ile' Tue Jun 30 18:40:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn797Ile' 6 unspecified role unspecified role prey prey c-terminal region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain The author is unsure if the region [2171-2647] is required for binding since a shorter region [2171-2647] shows a weak interaction, possibly explained by an insensitive two-hybrid detection system. This has led to the use of 'sufficient to bind' feature type. 19 9 physical association physical association Fig. 3,4,5,6,7 and 8. The human filamin consists of three isoforms. The protein included in this entry is Filamin A. flna-itgb1-3 3 4 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association Fig 2. The human filamin protein consists of three isoforms. The protein included in this entry is filamin A. flna-itgb1-7 1 6 unspecified role unspecified role neutral component neutral component purified purified c-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role neutral component neutral component c-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 28 25 direct interaction direct interaction Fig. 1. The human filamin consists of three isoforms. The protein included in this entry is filamin A.