UniProt UniProt http://www.uniprot.org Isolation and characterization of a novel coactivator protein, NCoA-62, involved in vitamin D-mediated transcription. Isolation and characterization of a novel coactivator protein, NCoA-62, involved in vitamin D-mediated transcription. J. Biol. Chem. (0021-9258) 1998 mimix curation Baudino T.A., Kraichely D.M., Jefcoat S.C., Winchester S.K., Partridge N.C., MacDonald P.N. Exp using RAR and RXR constructs omitted as the origin of these interactors cannot be established from the publication (fig 1, part. fig. 6). macdonal@slu.edu 18-Dec-2012: Contacted by IntAct-Help. in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting predetermined predetermined participant predetermined Baudino T.A., Kraichely D.M., Jefcoat S.C., Winchester S.K., Partridge N.C., MacDonald P.N. Exp using RAR and RXR constructs omitted as the origin of these interactors cannot be established from the publication (fig 1, part. fig. 6). macdonal@slu.edu Accepted 2012-NOV-19 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. J. Biol. Chem. (0021-9258) 1998 mimix curation Isolation and characterization of a novel coactivator protein, NCoA-62, involved in vitamin D-mediated transcription. Isolation and characterization of a novel coactivator protein, NCoA-62, involved in vitamin D-mediated transcription. J. Biol. Chem. (0021-9258) 1998 mimix curation Baudino T.A., Kraichely D.M., Jefcoat S.C., Winchester S.K., Partridge N.C., MacDonald P.N. Exp using RAR and RXR constructs omitted as the origin of these interactors cannot be established from the publication (fig 1, part. fig. 6). macdonal@slu.edu 18-Dec-2012: Contacted by IntAct-Help. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Baudino T.A., Kraichely D.M., Jefcoat S.C., Winchester S.K., Partridge N.C., MacDonald P.N. Exp using RAR and RXR constructs omitted as the origin of these interactors cannot be established from the publication (fig 1, part. fig. 6). macdonal@slu.edu Accepted 2012-NOV-19 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. HeLa cell cDNA J. Biol. Chem. (0021-9258) 1998 mimix curation Isolation and characterization of a novel coactivator protein, NCoA-62, involved in vitamin D-mediated transcription. Isolation and characterization of a novel coactivator protein, NCoA-62, involved in vitamin D-mediated transcription. J. Biol. Chem. (0021-9258) 1998 mimix curation Baudino T.A., Kraichely D.M., Jefcoat S.C., Winchester S.K., Partridge N.C., MacDonald P.N. Exp using RAR and RXR constructs omitted as the origin of these interactors cannot be established from the publication (fig 1, part. fig. 6). macdonal@slu.edu 18-Dec-2012: Contacted by IntAct-Help. in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Baudino T.A., Kraichely D.M., Jefcoat S.C., Winchester S.K., Partridge N.C., MacDonald P.N. Exp using RAR and RXR constructs omitted as the origin of these interactors cannot be established from the publication (fig 1, part. fig. 6). macdonal@slu.edu Accepted 2012-NOV-19 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. J. Biol. Chem. (0021-9258) 1998 mimix curation Isolation and characterization of a novel coactivator protein, NCoA-62, involved in vitamin D-mediated transcription. Isolation and characterization of a novel coactivator protein, NCoA-62, involved in vitamin D-mediated transcription. J. Biol. Chem. (0021-9258) 1998 mimix curation Baudino T.A., Kraichely D.M., Jefcoat S.C., Winchester S.K., Partridge N.C., MacDonald P.N. Exp using RAR and RXR constructs omitted as the origin of these interactors cannot be established from the publication (fig 1, part. fig. 6). macdonal@slu.edu 18-Dec-2012: Contacted by IntAct-Help. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Baudino T.A., Kraichely D.M., Jefcoat S.C., Winchester S.K., Partridge N.C., MacDonald P.N. Exp using RAR and RXR constructs omitted as the origin of these interactors cannot be established from the publication (fig 1, part. fig. 6). macdonal@slu.edu Accepted 2012-NOV-19 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. J. Biol. Chem. (0021-9258) 1998 mimix curation vdr_human Vitamin D3 receptor 1,25-dihydroxyvitamin D3 receptor Nuclear receptor subfamily 1 group I member 1 VDR NR1I1 protein protein human Homo sapiens Human MEAMAASTSLPDPGDFDRNVPRICGVCGDRATGFHFNAMTCEGCKGFFRRSMKRKALFTCPFNGDCRITKDNRRHCQACRLKRCVDIGMMKEFILTDEEVQRKREMILKRKEEEALKDSLRPKLSEEQQRIIAILLDAHHKTYDPTYSDFCQFRPPVRVNDGGGSHPSRPNSRHTPSFSGDSSSSCSDHCITSSDMMDSSSFSNLDLSEEDSDDPSVTLELSQLSMLPHLADLVSYSIQKVIGFAKMIPGFRDLTSEDQIVLLKSSAIEVIMLRSNESFTMDDMSWTCGNQDYKYRVSDVTKAGHSLELIEPLIKFQVGLKKLNLHEEEHVLLMAICIVSPDRPGVQDAALIEAIQDRLSNTLQTYIRCRHPPPGSHLLYAKMIQKLADLRSLNEEHSKQYRCLSFQPECSMKLTPLVLEVFGNEIS DIP protein P11473 original sequence version: 179 DIP protein P11473 original sequence version: 146 F95F300D042C4CB7 snw1_human SNW domain-containing protein 1 Nuclear protein SkiP Ski-interacting protein Nuclear receptor coactivator NCoA-62 SKIIP SKIP SNW1 protein protein human Homo sapiens Human MALTSFLPAPTQLSQDQLEAEEKARSQRSRQTSLVSSRREPPPYGYRKGWIPRLLEDFGDGGAFPEIHVAQYPLDMGRKKKMSNALAIQVDSEGKIKYDAIARQGQSKDKVIYSKYTDLVPKEVMNADDPDLQRPDEEAIKEITEKTRVALEKSVSQKVAAAMPVRAADKLAPAQYIRYTPSQQGVAFNSGAKQRVIRMVEMQKDPMEPPRFKINKKIPRGPPSPPAPVMHSPSRKMTVKEQQEWKIPPCISNWKNAKGYTIPLDKRLAADGRGLQTVHINENFAKLAEALYIADRKAREAVEMRAQVERKMAQKEKEKHEEKLREMAQKARERRAGIKTHVEKEDGEARERDEIRHDRRKERQHDRNLSRAAPDKRSKLQRNENRDISEVIALGVPNPRTSNEVQYDQRLFNQSKGMDSGFAGGEDEIYNVYDQAWRGGKDMAQSIYRPSKNLDKDMYGDDLEARIKTNRFVPDKEFSGSDRRQRGREGPVQFEEDPFGLDKFLEEAKQHGGSKRPSDSSRPKEHEHEGKKRRKE DIP protein Q13573 original sequence version: 156 0CC75E0D0B2CF842 vdr-snw1-7 2 5 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association no figure vdr-snw1-4 4 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association 1 and 5 vdr-snw1-3 3 5 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association 6B, 6D vdr-snw1-5 1 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association 6A