mbinfo MBInfo http://www.mechanobio.info/ A specific domain in alpha-catenin mediates binding to beta-catenin or plakoglobin. A specific domain in alpha-catenin mediates binding to beta-catenin or plakoglobin. J. Cell. Sci. (0021-9533) 1997 imex curation Huber O., Krohn M., Kemler R. Recombinant MBP-alpha-catenin and GST-beta-catenin were incubated in an in-vitro reconstitution assay and the reconstituted heterodimers were purified by by addition of glutathione-sepharose beads. The source of the proteins (alpha-catenin, beta-catenin and plakoglobin) not specified by the author. The source of alpha-catenin for MBP fusions and of beta-catenin for GST fusions inferred from references mentioned in the paper. Since sources for His-alpha-catenin fusions, His-beta-catenin fusions and GST-plakoglobin fusions could not be found, some experiments (Fig. 4, 5, 6A and 6C) could not be curated. kemler@immunbio.mpg.de Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Huber O., Krohn M., Kemler R. Recombinant MBP-alpha-catenin and GST-beta-catenin were incubated in an in-vitro reconstitution assay and the reconstituted heterodimers were purified by by addition of glutathione-sepharose beads. The source of the proteins (alpha-catenin, beta-catenin and plakoglobin) not specified by the author. The source of alpha-catenin for MBP fusions and of beta-catenin for GST fusions inferred from references mentioned in the paper. Since sources for His-alpha-catenin fusions, His-beta-catenin fusions and GST-plakoglobin fusions could not be found, some experiments (Fig. 4, 5, 6A and 6C) could not be curated. kemler@immunbio.mpg.de Accepted 2012-FEB-28 by ORCHARD J. Cell. Sci. (0021-9533) 1997 Only protein-protein interactions imex curation A specific domain in alpha-catenin mediates binding to beta-catenin or plakoglobin. A specific domain in alpha-catenin mediates binding to beta-catenin or plakoglobin. J. Cell. Sci. (0021-9533) 1997 imex curation Huber O., Krohn M., Kemler R. Recombinant MBP-alpha-catenin and GST-beta-catenin were incubated in an in-vitro reconstitution assay and the reconstituted heterodimers were purified by by addition of glutathione-sepharose beads. The source of the proteins (alpha-catenin, beta-catenin and plakoglobin) not specified by the author. The source of alpha-catenin for MBP fusions and of beta-catenin for GST fusions inferred from references mentioned in the paper. Since sources for His-alpha-catenin fusions, His-beta-catenin fusions and GST-plakoglobin fusions could not be found, some experiments (Fig. 4, 5, 6A and 6C) could not be curated. kemler@immunbio.mpg.de Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Huber O., Krohn M., Kemler R. Recombinant MBP-alpha-catenin and GST-beta-catenin were incubated in an in-vitro reconstitution assay and the reconstituted heterodimers were purified by by addition of glutathione-sepharose beads. The source of the proteins (alpha-catenin, beta-catenin and plakoglobin) not specified by the author. The source of alpha-catenin for MBP fusions and of beta-catenin for GST fusions inferred from references mentioned in the paper. Since sources for His-alpha-catenin fusions, His-beta-catenin fusions and GST-plakoglobin fusions could not be found, some experiments (Fig. 4, 5, 6A and 6C) could not be curated. kemler@immunbio.mpg.de Accepted 2012-FEB-28 by ORCHARD J. Cell. Sci. (0021-9533) 1997 Only protein-protein interactions imex curation A specific domain in alpha-catenin mediates binding to beta-catenin or plakoglobin. A specific domain in alpha-catenin mediates binding to beta-catenin or plakoglobin. J. Cell. Sci. (0021-9533) 1997 imex curation Huber O., Krohn M., Kemler R. Recombinant MBP-alpha-catenin and GST-beta-catenin were incubated in an in-vitro reconstitution assay and the reconstituted heterodimers were purified by by addition of glutathione-sepharose beads. The source of the proteins (alpha-catenin, beta-catenin and plakoglobin) not specified by the author. The source of alpha-catenin for MBP fusions and of beta-catenin for GST fusions inferred from references mentioned in the paper. Since sources for His-alpha-catenin fusions, His-beta-catenin fusions and GST-plakoglobin fusions could not be found, some experiments (Fig. 4, 5, 6A and 6C) could not be curated. kemler@immunbio.mpg.de Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Huber O., Krohn M., Kemler R. Recombinant MBP-alpha-catenin and GST-beta-catenin were incubated in an in-vitro reconstitution assay and the reconstituted heterodimers were purified by by addition of glutathione-sepharose beads. The source of the proteins (alpha-catenin, beta-catenin and plakoglobin) not specified by the author. The source of alpha-catenin for MBP fusions and of beta-catenin for GST fusions inferred from references mentioned in the paper. Since sources for His-alpha-catenin fusions, His-beta-catenin fusions and GST-plakoglobin fusions could not be found, some experiments (Fig. 4, 5, 6A and 6C) could not be curated. kemler@immunbio.mpg.de Accepted 2012-FEB-28 by ORCHARD J. Cell. Sci. (0021-9533) 1997 Only protein-protein interactions imex curation ctnb1_human Catenin beta-1 Beta-catenin OK/SW-cl.35 PRO2286 CTNNB1 CTNNB protein protein human Homo sapiens Human MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLDEGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDEDQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLSHHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQKMVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTTSRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGMEGLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDREDITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNLALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGALHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEGATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETADLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGLPDLGHAQDLMDGLPPGDSNQLAWFDTDL DIP protein P35222 original sequence version: 187 DIP protein P35222 original sequence version: 188 DIP protein P35222 original sequence version: 180 DIP protein P35222-1 original sequence version: 181 DIP protein P35222 original sequence version: 181 DIP protein P35222 original sequence version: 107 DIP protein P35222-1 original sequence version: 107 DIP protein P35222 original sequence version: 103 DIP protein P35222-1 original sequence version: 103 DIP protein P35222 original sequence version: 175 DIP protein P35222 original sequence version: 136 DIP protein P35222 original sequence version: 135 DIP protein P35222 original sequence version: 131 DIP protein P35222-1 original sequence version: 133 DIP protein P35222 original sequence version: 133 DIP protein P35222 original sequence version: 148 DIP protein P35222-1 original sequence version: 139 DIP protein P35222 original sequence version: 139 DIP protein P35222 original sequence version: 146 DIP protein P35222 original sequence version: 196 DIP protein P35222 original sequence version: 192 DIP protein P35222 original sequence version: 185 DIP protein P35222 original sequence version: 151 DIP protein P35222-1 original sequence version: 151 DIP protein P35222-1 original sequence version: 171 DIP protein P35222-1 original sequence version: 148 DIP protein P35222 original sequence version: 177 DIP protein P35222 original sequence version: 178 DIP protein P35222-1 original sequence version: 178 CB78F165A3EEF86E ctnb1_mouse Catenin beta-1 Beta-catenin Ctnnb1 Catnb protein protein mouse Mus musculus Mouse MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLDEGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDEDQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLSHHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQKMVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTTSRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGMEGLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDREDITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNLALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGALHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEGATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETADLGLDIGAQGEALGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGLPDLGHAQDLMDGLPPGDSNQLAWFDTDL DIP protein Q02248 original sequence version: 128 DIP protein Q02248 original sequence version: 135 DIP protein Q02248 original sequence version: 147 DIP protein Q02248 original sequence version: 187 DIP protein Q02248 original sequence version: 140 D708F170A3FBED6E ctna1_mouse Catenin alpha-1 102 kDa cadherin-associated protein CAP102 Alpha E-catenin Ctnna1 Catna1 protein protein mouse Mus musculus Mouse MTAVHAGNINFKWDPKSLEIRTLAVERLLEPLVTQVTTLVNTNSKGPSNKKRGRSKKAHVLAASVEQATENFLEKGDKIAKESQFLKEELVVAVEDVRKQGDLMKSAAGEFADDPCSSVKRGNMVRAARALLSAVTRLLILADMADVYKLLVQLKVVEDGILKLRNAGNEQDLGIQYKALKPEVDKLNIMAAKRQQELKDVGNRDQMAAARGILQKNVPILYTASQACLQHPDVAAYKANRDLIYKQLQQAVTGISNAAQATASDDAAQHQGGSGGELAYALNNFDKQIIVDPLSFSEERFRPSLEERLESIISGAALMADSSCTRDDRRERIVAECNAVRQALQDLLSEYMGNAGRKERSDALNSAIDKMTKKTRDLRRQLRKAVMDHVSDSFLETNVPLLVLIEAAKNGNEKEVKEYAQVFREHANKLIEVANLACSISNNEEGVKLVRMSASQLEALCPQVINAALALAAKPQSKLAQENMDLFKEQWEKQVRVLTDAVDDITSIDDFLAVSENHILEDVNKCVIALQEKDVDGLDRTAGAIRGRAARVIHVVTSEMDNYEPGVYTEKVLEATKLLSNTVMPRFTEQVEAAVEALSSDPAQPMDENEFIDASRLVYDGIRDIRKAVLMIRTPEELDDSDFETEDFDVRSRTSVQTEDDQLIAGQSARAIMAQLPQEQKAKIAEQVASFQEEKSKLDAEVSKWDDSGNDIIVLAKQMCMIMMEMTDFTRGKGPLKNTSDVISAAKKIAEAGSRMDKLGRTIADHCPDSACKQDLLAYLQRIALYCHQLNICSKVKAEVQNLGGELVVSGVDSAMSLIQAAKNLMNAVVQTVKASYVASTKYQKSQGMASLNLPAVSWKMKAPEKKPLVKREKQDETQTKIKRASQKKHVNPVQALSEFKAMDSI DIP protein P26231 original sequence version: 125 DIP protein P26231 original sequence version: 142 DIP protein P26231 original sequence version: 160 3ED1EC50925DBBB5 plak_human Junction plakoglobin JUP DP3 CTNNG Desmoplakin-3 Desmoplakin III Catenin gamma protein protein human Homo sapiens Human MEVMNLMEQPIKVTEWQQTYTYDSGIHSGANTCVPSVSSKGIMEEDEACGRQYTLKKTTTYTQGVPPSQGDLEYQMSTTARAKRVREAMCPGVSGEDSSLLLATQVEGQATNLQRLAEPSQLLKSAIVHLINYQDDAELATRALPELTKLLNDEDPVVVTKAAMIVNQLSKKEASRRALMGSPQLVAAVVRTMQNTSDLDTARCTTSILHNLSHHREGLLAIFKSGGIPALVRMLSSPVESVLFYAITTLHNLLLYQEGAKMAVRLADGLQKMVPLLNKNNPKFLAITTDCLQLLAYGNQESKLIILANGGPQALVQIMRNYSYEKLLWTTSRVLKVLSVCPSNKPAIVEAGGMQALGKHLTSNSPRLVQNCLWTLRNLSDVATKQEGLESVLKILVNQLSVDDVNVLTCATGTLSNLTCNNSKNKTLVTQNSGVEALIHAILRAGDKDDITEPAVCALRHLTSRHPEAEMAQNSVRLNYGIPAIVKLLNQPNQWPLVKATIGLIRNLALCPANHAPLQEAAVIPRLVQLLVKAHQDAQRHVAAGTQQPYTDGVRMEEIVEGCTGALHILARDPMNRMEIFRLNTIPLFVQLLYSSVENIQRVAAGVLCELAQDKEAADAIDAEGASAPLMELLHSRNEGTATYAAAVLFRISEDKNPDYRKRVSVELTNSLFKHDPAAWEAAQSMIPINEPYGDDMDATYRPMYSSDVPLDPLEMHMDMDGDYPIDTYSDGLRPPYPTADHMLA DIP protein P14923 original sequence version: 159 3519A0973748BCF4 ctnna1-ctnnb1-3 3 6 unspecified role unspecified role prey prey n-terminal region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Fig. 1 and 2. Mouse alpha-catenin has three isoforms. The protein included in this entry is alpha-catenin 1. MBP-alpha-catenin was expressed in E.coli BL21 and GST-beta-catenin was expressed in E.coli XL-1 cells (taken from reference mentioned by the author). ctnna1-ctnnb1-5 2 6 unspecified role unspecified role bait bait P26231:p.Ala142Ser mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain A S Wed May 02 17:35:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala142ser' Tue Jun 30 19:07:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala142Ser' region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P26231:p.Leu139Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L A Wed May 02 18:23:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu139ala' Tue Jun 30 15:51:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu139Ala' P26231:p.Thr136Ala mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain T A Wed May 02 16:38:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr136ala' Tue Jun 30 20:14:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr136Ala' P26231:p.Ser133Ala mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A Wed May 02 15:04:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser133ala' Tue Jun 30 16:42:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser133Ala' P26231:p.Leu132Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L A Wed May 02 18:35:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu132ala' Tue Jun 30 19:46:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu132Ala' n-terminal region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain P26231:p.Leu141Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L A Wed May 02 16:13:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu141ala' Tue Jun 30 18:13:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu141Ala' P26231:p.Leu138Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L A Wed May 02 17:18:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu138ala' Wed May 02 20:29:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu138ala' Tue Jun 30 18:15:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu138Ala' P26231:p.Arg137Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A Wed May 02 20:01:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg137ala' Tue Jun 30 17:58:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg137Ala' P26231:p.Val135Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain V A Wed May 02 19:15:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val135ala' Tue Jun 30 19:15:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val135Ala' P26231:p.Leu131Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L A Wed May 02 17:45:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu131ala' Tue Jun 30 15:51:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu131Ala' P26231:p.Arg129Ala mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A Wed May 02 16:30:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg129ala' Tue Jun 30 17:41:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg129Ala' P26231:p.Arg126Ala mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A Wed May 02 20:10:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg126ala' Tue Jun 30 15:59:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg126Ala' P26231:p.Val125Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain V A Wed May 02 19:43:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val125ala' Tue Jun 30 16:33:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val125Ala' ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-sw480 Human SW480 adenocarcinoma cell line sw480 SW480- human primary colorectal adenocarcinoma cell line There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution and a C -> T mutation in codon 309 resulting in a Pro -> Ser substitution. http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=CCL-228&Template=cellBiology physical association physical association The mouse alpha-catenin has three isoforms. The protein included in this entry is alpha-catenin 1. Fig. 3B and 7. ctnna1-jup-1 1 6 unspecified role unspecified role bait bait region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-sw480 Human SW480 adenocarcinoma cell line sw480 SW480- human primary colorectal adenocarcinoma cell line There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution and a C -> T mutation in codon 309 resulting in a Pro -> Ser substitution. http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=CCL-228&Template=cellBiology physical association physical association Fig. 6B. The mouse alpha-actinin has three isoforms. The protein included in this entry is alpha-actinin 1. ctnna1-ctnnb1-7 1 4 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-sw480 Human SW480 adenocarcinoma cell line sw480 SW480- human primary colorectal adenocarcinoma cell line There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution and a C -> T mutation in codon 309 resulting in a Pro -> Ser substitution. http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=CCL-228&Template=cellBiology 6 unspecified role unspecified role bait bait n-terminal region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Fig. 3B. The mouse alpha-catenin has three isoforms. The protein included in this entry is alpha-catenin 1.