mbinfo MBInfo http://www.mechanobio.info/ Identification of the vinculin-binding site in the cytoskeletal protein alpha-actinin. Identification of the vinculin-binding site in the cytoskeletal protein alpha-actinin. Biochem. J. (0264-6021) 1994 imex curation McGregor A., Blanchard A.D., Rowe A.J., Critchley D.R. Interaction detection method: GST-pull down. The source of both alpha-actinin and vinculin specified by the author. Contact-email not provided in the publication. Only protein-protein interactions in vitro In vitro electron microscopy electron microscopy Electron crystallography Electron cryomicroscopy Electron crystallography Electron cryomicroscopy predetermined predetermined participant predetermined McGregor A., Blanchard A.D., Rowe A.J., Critchley D.R. Interaction detection method: GST-pull down. The source of both alpha-actinin and vinculin specified by the author. Contact-email not provided in the publication. Accepted 2012-MAR-29 by ORCHARD Biochem. J. (0264-6021) 1994 Only protein-protein interactions imex curation Identification of the vinculin-binding site in the cytoskeletal protein alpha-actinin. Identification of the vinculin-binding site in the cytoskeletal protein alpha-actinin. Biochem. J. (0264-6021) 1994 imex curation McGregor A., Blanchard A.D., Rowe A.J., Critchley D.R. Interaction detection method: GST-pull down. The source of both alpha-actinin and vinculin specified by the author. Contact-email not provided in the publication. Only protein-protein interactions in vitro In vitro pull down pull down autoradiography autoradiography McGregor A., Blanchard A.D., Rowe A.J., Critchley D.R. Interaction detection method: GST-pull down. The source of both alpha-actinin and vinculin specified by the author. Contact-email not provided in the publication. Accepted 2012-MAR-29 by ORCHARD Biochem. J. (0264-6021) 1994 Only protein-protein interactions imex curation Identification of the vinculin-binding site in the cytoskeletal protein alpha-actinin. Identification of the vinculin-binding site in the cytoskeletal protein alpha-actinin. Biochem. J. (0264-6021) 1994 imex curation McGregor A., Blanchard A.D., Rowe A.J., Critchley D.R. Interaction detection method: GST-pull down. The source of both alpha-actinin and vinculin specified by the author. Contact-email not provided in the publication. Only protein-protein interactions in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting autoradiography autoradiography McGregor A., Blanchard A.D., Rowe A.J., Critchley D.R. Interaction detection method: GST-pull down. The source of both alpha-actinin and vinculin specified by the author. Contact-email not provided in the publication. Accepted 2012-MAR-29 by ORCHARD Biochem. J. (0264-6021) 1994 Only protein-protein interactions imex curation Identification of the vinculin-binding site in the cytoskeletal protein alpha-actinin. Identification of the vinculin-binding site in the cytoskeletal protein alpha-actinin. Biochem. J. (0264-6021) 1994 imex curation McGregor A., Blanchard A.D., Rowe A.J., Critchley D.R. Interaction detection method: GST-pull down. The source of both alpha-actinin and vinculin specified by the author. Contact-email not provided in the publication. Only protein-protein interactions in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati weight identificat confirmation by molecular weight weight identificat McGregor A., Blanchard A.D., Rowe A.J., Critchley D.R. Interaction detection method: GST-pull down. The source of both alpha-actinin and vinculin specified by the author. Contact-email not provided in the publication. Accepted 2012-MAR-29 by ORCHARD Biochem. J. (0264-6021) 1994 Only protein-protein interactions imex curation vinc_chick Vinculin VCL VINC1 Metavinculin protein protein chick Gallus gallus (Chicken) Chicken MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVSAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVRAAQMLQADPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNTKSQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALALIDSKMNQAKGWLRDPNAPPGDAGEQAIRQILDEAGKAGELCAGKERREILGTCKTLGQMTDQLADLRARGQGATPMAMQKAQQVSQGLDLLTAKVENAARKLEAMTNSKQAIAKKIDAAQNWLADPNGGSEGEEHIRGIMSEARKVAELCEEPKERDDILRSLGEISALTAKLSDLRRHGKGDSPEARALAKQIATSLQNLQSKTNRAVANTRPVKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGRRLANVMMGPYRQDLLAKCDRVDQLAAQLADLAARGEGESPQARAIAAQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPSDTPNREEVFEERAANFENHAARLGATAEKAAAVGTANKTTVEGIQATVKSARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANMQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEHLHLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEAINQPMMMAARQLHDEARKWSSKPVTVINEAAEAGVDIDEEDDADVEFSLPSDIEDDYEPELLLMPTNQPVNQPILAAAQSLHREATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGNKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ DIP protein P12003 original sequence version: 104 DIP protein P12003-2 original sequence version: 135 DIP protein P12003 original sequence version: 146 28CC2699C9511058 p05094-2 Alpha-actinin-1 Smooth muscle ACTN1 Alpha-actinin cytoskeletal isoform F-actin cross-linking protein Non-muscle alpha-actinin-1 protein protein chick Gallus gallus (Chicken) Chicken MDHHYDPQQTNDYMQPEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRAPENTMQAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGKMVSDINNAWGGLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHESWTDGKEAMLQQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERQAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWEHVRQLVPRRDQALMEEHARQQQNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLNHLRQYEKSIVNYKPKIDQLEGDHQQIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRKKTGMMDCEDFRACLISMGYNMGEAEFARIMSIVDPNRMGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITVDELRRELPPDQAEYCIARMAPYNGRDAVPGALDYMSFSTALYGESDL 5F2485A047EA644C actn1-vcl-7 2 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined central region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role prey prey region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chick-gizzard Chicken gizzard gizzard gizzard physical association physical association Table 1. The chick alpha-actinin has multiple isoforms. The protein included in this entry is alpha-actinin 1. actn1-vcl-8 3 6 unspecified role unspecified role prey prey central region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain chick Gallus gallus (Chicken) Chicken 5 unspecified role unspecified role bait bait region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chick Gallus gallus (Chicken) Chicken physical association physical association Fig 3 (a-f) and 4A. The chick alpha-actinin has multiple isoforms. The protein included in this entry is alpha-actinin 1. actn1-vcl-9 3 6 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined central region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role bait bait region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chick Gallus gallus (Chicken) Chicken physical association physical association Fig 3(g-l), 4B, 5b, 5c, 6b, 6d and 6e. actn1-vcl-10 1 6 unspecified role unspecified role neutral component neutral component chick Gallus gallus (Chicken) Chicken 5 unspecified role unspecified role neutral component neutral component chick Gallus gallus (Chicken) Chicken physical association physical association Fig 2c. The chick alpha-actinin has multiple isoforms. The protein included in this entry is alpha-actinin 1. actn1-vcl-11 4 6 unspecified role unspecified role neutral component neutral component chick Gallus gallus (Chicken) Chicken 5 unspecified role unspecified role neutral component neutral component chick Gallus gallus (Chicken) Chicken physical association physical association Fig 1. The chick alpha-actinin has multiple isoforms. The protein included in this entry is alpha-actinin 1.