IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Oligomeric structure of Escherichia coli primary replicative helicase DnaB protein. Oligomeric structure of Escherichia coli primary replicative helicase DnaB protein. J. Biol. Chem. (0021-9258) 1994 imex curation Bujalowski W., Klonowska M.M., Jezewska M.J. wbujalow@utmb.edu 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions ecoli Escherichia coli (strain K12) solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Bujalowski W., Klonowska M.M., Jezewska M.J. wbujalow@utmb.edu Accepted 2012-DEC-05 by MGT 18-Dec-2012: Contacted by IntAct-Help. J. Biol. Chem. (0021-9258) 1994 Only protein-protein interactions imex curation Oligomeric structure of Escherichia coli primary replicative helicase DnaB protein. Oligomeric structure of Escherichia coli primary replicative helicase DnaB protein. J. Biol. Chem. (0021-9258) 1994 imex curation Bujalowski W., Klonowska M.M., Jezewska M.J. wbujalow@utmb.edu 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions ecoli Escherichia coli (strain K12) fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Bujalowski W., Klonowska M.M., Jezewska M.J. wbujalow@utmb.edu Accepted 2012-DEC-05 by MGT 18-Dec-2012: Contacted by IntAct-Help. J. Biol. Chem. (0021-9258) 1994 Only protein-protein interactions imex curation dnab_ecoli Replicative DNA helicase dnaB groP grpA b4052 JW4012 protein protein ecoli Escherichia coli (strain K12) MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLDNERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGGFAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDLAESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKTAGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASLSRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREHGGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVRLTFNGQWSRFDNYAGPQYDDE 5B5ED3625A7F7DE5 mant-adp 3'-O-(N-methylanthraniloyl)adenosine 5'-diphosphate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) dnab-hexamer 1 3 unspecified role unspecified role neutral component neutral component purified purified colocalization colocalization Fig.s 1, 3 homohexamer, unwinds double-stranded DNA at replication fork DnaB forms hexamers in the presence of MgCl2 dnab-mant-adp 2 4 unspecified role unspecified role neutral component neutral component 3 unspecified role unspecified role neutral component neutral component purified purified physical association physical association Fig. 2 homohexamer, unwinds double-stranded DNA at replication fork primary replicative helicase; DnaB protein dnab-trimer 1 3 unspecified role unspecified role neutral component neutral component purified purified colocalization colocalization Fig. 3 DnaB forms trimers in the absence of MgCl2