MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Functional expression, purification and reconstitution of the recombinant phosphate transporter Pho89 of Saccharomyces cerevisiae. Functional expression, purification and reconstitution of the recombinant phosphate transporter Pho89 of Saccharomyces cerevisiae. FEBS J. (1742-464X) 2013 Sengottaiyan P., Ruiz-Pavón L., Persson BL. suggested by lperfetto in vitro In vitro comigration in gel comigration in gel electrophoresis comigration in gel anti tag western anti tag western blot anti tag western Sengottaiyan P., Ruiz-Pavón L., Persson BL. suggested by lperfetto FEBS J. (1742-464X) 2013 Functional expression, purification and reconstitution of the recombinant phosphate transporter Pho89 of Saccharomyces cerevisiae. Functional expression, purification and reconstitution of the recombinant phosphate transporter Pho89 of Saccharomyces cerevisiae. FEBS J. (1742-464X) 2013 Sengottaiyan P., Ruiz-Pavón L., Persson BL. suggested by lperfetto in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column anti tag western anti tag western blot anti tag western Sengottaiyan P., Ruiz-Pavón L., Persson BL. suggested by lperfetto FEBS J. (1742-464X) 2013 pho89_yeast Phosphate permease PHO89 PHO89 ITN1 YBR296C YBR2113 Na(+)/Pi cotransporter PHO89 protein protein yeast Saccharomyces cerevisiae Baker's yeast MALHQFDYIFAIAMLFAFLDAFNIGANDVANSFASSISSRSLKYWQAMVLAGLCEFLGAVLAGARVSGTIKNNIIDSSIFTNDPAVLMLTMTSALIGSSCWLTFATAIGMPVSTTHSIVGGTIGAGIAAGGANGVVWGWSGVSQIIASWFIAPILAGAIAAIVFSISRFSVLEVKSLERSIKNALLLVGVLVFATFSILTMLIVWKGSPNLHLDDLSETETAVSIVLTGAIASIVYFIFFYPFYRRKVLDQDWTLKLIDIFRGPSFYFKSTDDIPPMPEGHQLTIDYYEGRRNLGTTVSVEDEENKAASNSNDSVKNKEDIQEVDLVRTETEPETKLSTKQYWWSLLKQGPKKWPLLFWLVISHGWTQDVIHAQVNDRDMLSGDLKGMYERSKFYDNRVEYIYSVLQAITAATMSFAHGANDVANATGPLSAVYVIWKTNTIGAKSEVPVWVLAYGGVALVIGCWTYGYNIIKNLGNKMILQSPSRGFSIELAVAITTVMATQLGIPTSTTQIAVGGIVAVGLCNKDLKSVNWRMVAWCYSGWFLTLPIAGLIAGIINGIILNAPRFGVEYQMT 9794C1D9D75014D5 9794C1D9D75014D5 pho89-pho89 2 Pho89 3 anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified tandem tag tandem tag tandem tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Pho89 3 anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified tandem tag tandem tag tandem tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction homomint mint f5 sf2c The size exclusion profile showed that the Pho89 was eluted as a single peak from the gel-filtration column as shown in Fig. 5A. Relative to protein standards, our results clearly indicate that the purified Pho89 is in a 520 kDa oligomeric form. Analysis of the oligomer subjected to SDS-PAGE (Fig. 5B and Fig. S2B), followed by Western blot analysis revealed that the signal correspond to the size of the 63 kDa monomer, the 140 kDa dimer and the 520 kDa oligomeric species of the Pho89 (Fig. 5C and Fig. S2C) pho89-1 2 Pho89 3 anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified tandem tag tandem tag tandem tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 2.0 direct interaction direct interaction homomint mint f5c sf2c pho89-1 1 Pho89 3 anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified tandem tag tandem tag tandem tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 2.0 direct interaction direct interaction sf3a homomint mint treatment of purified Pho89 with DTT (100 mM) does not affect their dimer/oligomer formation (Fig. S3A, B). pho89-pho89-1 1 Pho89 3 anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified tandem tag tandem tag tandem tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Pho89 3 anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified tandem tag tandem tag tandem tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction sf3a homomint mint treatment of purified Pho89 with DTT (100 mM) does not affect their dimer/oligomer formation (Fig. S3A, B).