MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it NDRG2 and PRA1 interact and synergistically inhibit T-cell factor/β-catenin signaling. NDRG2 and PRA1 interact and synergistically inhibit T-cell factor/β-catenin signaling. FEBS Lett. (0014-5793) 2012 Kim JT., Kim JW., Kang YH., Kim KD., Lee SJ., Choi SC., Kim KS., Chae SK., Kim JW., Lim JS., Lee HG. in vitro In vitro fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Kim JT., Kim JW., Kang YH., Kim KD., Lee SJ., Choi SC., Kim KS., Chae SK., Kim JW., Lim JS., Lee HG. FEBS Lett. (0014-5793) 2012 NDRG2 and PRA1 interact and synergistically inhibit T-cell factor/β-catenin signaling. NDRG2 and PRA1 interact and synergistically inhibit T-cell factor/β-catenin signaling. FEBS Lett. (0014-5793) 2012 Kim JT., Kim JW., Kang YH., Kim KD., Lee SJ., Choi SC., Kim KS., Chae SK., Kim JW., Lim JS., Lee HG. in vitro In vitro pull down pull down predetermined predetermined participant predetermined Kim JT., Kim JW., Kang YH., Kim KD., Lee SJ., Choi SC., Kim KS., Chae SK., Kim JW., Lim JS., Lee HG. FEBS Lett. (0014-5793) 2012 NDRG2 and PRA1 interact and synergistically inhibit T-cell factor/β-catenin signaling. NDRG2 and PRA1 interact and synergistically inhibit T-cell factor/β-catenin signaling. FEBS Lett. (0014-5793) 2012 Kim JT., Kim JW., Kang YH., Kim KD., Lee SJ., Choi SC., Kim KS., Chae SK., Kim JW., Lim JS., Lee HG. yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kim JT., Kim JW., Kang YH., Kim KD., Lee SJ., Choi SC., Kim KS., Chae SK., Kim JW., Lim JS., Lee HG. FEBS Lett. (0014-5793) 2012 NDRG2 and PRA1 interact and synergistically inhibit T-cell factor/β-catenin signaling. NDRG2 and PRA1 interact and synergistically inhibit T-cell factor/β-catenin signaling. FEBS Lett. (0014-5793) 2012 Kim JT., Kim JW., Kang YH., Kim KD., Lee SJ., Choi SC., Kim KS., Chae SK., Kim JW., Lim JS., Lee HG. human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Kim JT., Kim JW., Kang YH., Kim KD., Lee SJ., Choi SC., Kim KS., Chae SK., Kim JW., Lim JS., Lee HG. FEBS Lett. (0014-5793) 2012 NDRG2 and PRA1 interact and synergistically inhibit T-cell factor/β-catenin signaling. NDRG2 and PRA1 interact and synergistically inhibit T-cell factor/β-catenin signaling. FEBS Lett. (0014-5793) 2012 Kim JT., Kim JW., Kang YH., Kim KD., Lee SJ., Choi SC., Kim KS., Chae SK., Kim JW., Lim JS., Lee HG. in vitro In vitro cosedimentation cosedimentation western blot western blot Immuno blot Immuno blot Kim JT., Kim JW., Kang YH., Kim KD., Lee SJ., Choi SC., Kim KS., Chae SK., Kim JW., Lim JS., Lee HG. FEBS Lett. (0014-5793) 2012 ctnb1_human Catenin beta-1 Beta-catenin OK/SW-cl.35 PRO2286 CTNNB1 CTNNB protein protein human Homo sapiens Human MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLDEGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDEDQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLSHHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQKMVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTTSRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGMEGLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDREDITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNLALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGALHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEGATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETADLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGLPDLGHAQDLMDGLPPGDSNQLAWFDTDL DIP protein P35222 original sequence version: 187 DIP protein P35222 original sequence version: 188 DIP protein P35222 original sequence version: 180 DIP protein P35222-1 original sequence version: 181 DIP protein P35222 original sequence version: 181 DIP protein P35222 original sequence version: 107 DIP protein P35222-1 original sequence version: 107 DIP protein P35222 original sequence version: 103 DIP protein P35222-1 original sequence version: 103 DIP protein P35222 original sequence version: 175 DIP protein P35222 original sequence version: 136 DIP protein P35222 original sequence version: 135 DIP protein P35222 original sequence version: 131 DIP protein P35222-1 original sequence version: 133 DIP protein P35222 original sequence version: 133 DIP protein P35222 original sequence version: 148 DIP protein P35222-1 original sequence version: 139 DIP protein P35222 original sequence version: 139 DIP protein P35222 original sequence version: 146 DIP protein P35222 original sequence version: 196 DIP protein P35222 original sequence version: 192 DIP protein P35222 original sequence version: 185 DIP protein P35222 original sequence version: 151 DIP protein P35222-1 original sequence version: 151 DIP protein P35222-1 original sequence version: 171 DIP protein P35222-1 original sequence version: 148 DIP protein P35222 original sequence version: 177 DIP protein P35222 original sequence version: 178 DIP protein P35222-1 original sequence version: 178 CB78F165A3EEF86E praf1_human Prenylated Rab acceptor protein 1 PRA1 family protein 1 RABAC1 PRA1 PRAF1 protein protein human Homo sapiens Human MAAQKDQQKDAEAEGLSGTTLLPKLIPSGAGREWLERRRATIRPWSTFVDQQRFSRPRNLGELCQRLVRNVEYYQSNYVFVFLGLILYCVVTSPMLLVALAVFFGACYILYLRTLESKLVLFGREVSPAHQYALAGGISFPFFWLAGAGSAVFWVLGATLVVIGSHAAFHQIEAVDGEELQMEPV A85BD5BDCF6C23E4 q9un36-1 Protein NDRG2 NDRG2 KIAA1248 N-myc downstream-regulated gene 2 protein Protein Syld709613 SYLD NDRG2ins protein protein human Homo sapiens Human MAELQEVQITEEKPLLPGQTPEAAKEAELAARILLDQGQTHSVETPYGSVTFTVYGTPKPKRPAILTYHDVGLNYKSCFQPLFQFEDMQEIIQNFVRVHVDAPGMEEGAPVFPLGYQYPSLDQLADMIPCVLQYLNFSTIIGVGVGAGAYILARYALNHPDTVEGLVLINIDPNAKGWMDWAAHKLTGLTSSIPEMILGHLFSQEELSGNSELIQKYRNIITHAPNLDNIELYWNSYNNRRDLNFERGGDITLRCPVMLVVGDQAPHEDAVVECNSKLDPTQTSFLKMADSGGQPQLTQPGKLTEAFKYFLQGMGYMASSCMTRLSRSRTASLTSAASVDGNRSRSRTLSQSSESGTLSSGPPGHTMEVSC mint homomint 7B49B0B12BD3F595 ndrg2-rabac1 3 NDRG2a 8 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined PRA1 7 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association homomint mint nf ndrg2-rabac1-1 3 NDRG2a 8 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell binding site binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined PRA1 7 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association f1 homomint mint As shown in Fig. 1B, NDRG2a exhibited strong binding to PRA1, whereas NDRG2H and NDRG2C weakly bound, and NDRG2N was not interactive. The transmembrane-containing C-terminal region of PRA1 was necessary for binding to NDRG2a, and mutation in C-terminal Val185 of PRA1 was not affected to the interaction of NDRG2-PRA1. It is known that the Cterminal Val185 residue of PRA1 is essential for dimerization and endoplasmic reticular (ER) export [17]. On the other hand, N-terminal transmembrane-deleted (PRA1N1, -N2) and selected deletion mutants (PRA1TM1, -TM2, -N3) of PRA1 showed weak or no interaction with NDRG2a, suggesting that PRA1 protein structure is important for binding. The interaction of LexA-PRA1 or B42-PRA1 resulted in strong β-galactosidas activity, as did LexA-NDRG2a or B42-NDRG2b. ndrg2-rabac1-2 2 NDRG2a 8 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined PRA1 7 weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra predetermined predetermined participant predetermined unspecified role unspecified role prey prey in vitro translated in vitro translated protein in vitro translated direct interaction direct interaction f3a homomint mint rabac1-ndrg2 4 NDRG2a 8 monoclonal western monoclonal antibody western blot monoclonal western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell PRA1 7 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f3b homomint mint ndrg2-rabac1-3 1 NDRG2a 8 immunostaining immunostaining predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell PRA1 7 tag fluorescence tag visualisation by fluorescence tag fluorescence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain colocalization colocalization homomint mint f3f f3g Confocal microscopy in HCT116 cells shows a colocalized distribution of NDRG2 and PRA1 proteins in the cytoplasm near the outer surface of the nucleus (Fig. 3C-c, -d, and -f). In cells cotransfected with GFP-NDRG2a and PRA1-ΔVal (Fig. 3C-e), colocalization is observed; however, this is not the case with transmembrane-deleted PRA1ΔTM (Fig. 3C-g), suggesting that NDRG2 may colocalize in the ER and Golgi complex with PRA1. ndrg2-rabac1-4 1 NDRG2a 8 tag fluorescence tag visualisation by fluorescence tag fluorescence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined PRA1 7 immunostaining immunostaining predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization homomint mint Confocal microscopy in HCT116 cells shows a colocalized distribution of NDRG2 and PRA1 proteins in the cytoplasm near the outer surface of the nucleus (Fig. 3C-c, -d, and -f). In cells cotransfected with GFP-NDRG2a and PRA1-ΔVal (Fig. 3C-e), colocalization is observed; however, this is not the case with transmembrane-deleted PRA1ΔTM (Fig. 3C-g), suggesting that NDRG2 may colocalize in the ER and Golgi complex with PRA1. f3c f3d f3e ndrg2-rabac1-5 1 NDRG2a 8 anti tag immunost anti tag immunostaining anti tag immunost predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined PRA1 7 tag fluorescence tag visualisation by fluorescence tag fluorescence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization f5a homomint mint ctnnb1-ndrg2 5 NDRG2a 8 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component subcellular prep subcellular preparation subcellular prep over-expressed over expressed level over-expressed PRA1 7 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component subcellular prep subcellular preparation subcellular prep over-expressed over expressed level over-expressed Catenin beta 6 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level colocalization colocalization f4e homomint mint