MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Structure and mechanism of an inverting alkylsulfatase from Pseudomonas sp. DSM6611 specific for secondary alkyl sulfates. Structure and mechanism of an inverting alkylsulfatase from Pseudomonas sp. DSM6611 specific for secondary alkyl sulfates. FEBS J. (1742-464X) 2012 Knaus T., Schober M., Kepplinger B., Faccinelli M., Pitzer J., Faber K., Macheroux P., Wagner U. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction experimental particp experimental participant identification experimental particp Knaus T., Schober M., Kepplinger B., Faccinelli M., Pitzer J., Faber K., Macheroux P., Wagner U. FEBS J. (1742-464X) 2012 f8kay7_9psed pisa1 protein protein pseudomonas sp. dsm 6611 Pseudomonas sp. DSM 6611 MSRFIRASQRRTLLATLIAATLAQPLLAAESLDSKPASAITAAKNAEVLKNLPFADREEFEAAKRGLIAPFSGQIKNAEGQVVWDMGAYQFLNDKDAADTVNPSLWHQAQLNNIAGLFEVMPKLYQVRGLDPANMTIIEGDSGLVLIDTLTTAETARAALDLYFQHRPKKPIVAVVYSHSHIDHFGGARGIIDEADVKAGKVKVFAPSGFMEHAVSENILAGTAMARRGQYQSGVMVPRGAQAQVDSGLFKTTATNATNTLVAPNVLIEKPYERHTVDGVELEFQLTLGSEAPSDMNIYLPQFKVLNTADNAPPAMHNLLTPRGAEVRDAKAWAGYIDASLEKYGDRTDVLIQQHNWPVWGGDKVRTYLADQRDMYAFLNNRALNLMNKGLTLHEIAAEVSKLPGELDRKWYLRSYYGALSTNLRAVYQRYLGFYDGNPANLDPFPPVEAGKRYVEAMGGADAVLKQMRAAIDKGDYRWAVQLGNHLVFADPANKDARALQADAMEQLGYQTENALWRNMYMTGAMELRHGVPTYDSRGKSEMGRALTPDMFFDLLAIRLDTDKAVGHDMTLNWVFEDLKQDIALTLRNGVLTQRVGSLNPKADVTVKLTKPTLDQIAARKLDLPTAIKQGTVKLDGDGKKLGEFFGLLDSFSPKFNIVEPLE mint 682966688172CBA1 pisa1 1 Pisa1 2 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 direct interaction direct interaction mint Pisa1 and Ser233Tyr/Phe250Gly double variant crystals were grown at 20 °C by sitting-drop vapor-diffusion in silica hydrogel with equal volume (5 μl) of protein (10 mg/ml) and reservoir solution (acetate buffer pH 4.6, and either 30% PEG 2000 or PEG 4000). Fragile leaflet shaped orthorhombic crystals with six molecules in the asymmetric unit were obtained using PEG 2000 (wt form-I) whereas PEG 4000 produced rodlike trigonal crystals hosting a dimer in the asymmetric unit (wt form-II). These crystals diffracted to 3.0 Å (double variant Ser233Tyr/Phe250Gly) and 2.7 Å (wt form-I and II) resolution, respectively, and were used for structure determination. Atomic coordinates and structure factors were deposited in the PDBe under accession codes 2yhe (wt form-I), 4av7 (double mutant) and 4axh (wt form-II). t1 f2 f3