IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ A "Twist box" Code of p53 Inactivation: Twist box:p53 Interaction Promotes p53 Degradation. A "Twist box" Code of p53 Inactivation: Twist box:p53 Interaction Promotes p53 Degradation. Cancer Cell (1535-6108) 2012 imex curation Piccinin S., Tonin E., Sessa S., Demontis S., Rossi S., Pecciarini L., Zanatta L., Pivetta F., Grizzo A., Sonego M., Rosano C., Dei Tos A.P., Doglioni C., Maestro R. Cancer - Interactions investigated in the context of cancer Dear Checker: this paper was "a pain" esp. for species/deletion/mutants - let me know if you wish to see the authors email (a very prompt reply....) -it might clarify things (??) The authors confirm that the interaction between Human p53 (and mutants) and full length Twist1 were confirmed for Human, Rat and Mouse Twist1. However Twist1 mutants are of Murine origin and P300 and Twist2 are of Human origin. maestro@cro.it, spiccinin@cro.it 15-Nov-2012: Contacted by pporras. Only protein-protein interactions in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Piccinin S., Tonin E., Sessa S., Demontis S., Rossi S., Pecciarini L., Zanatta L., Pivetta F., Grizzo A., Sonego M., Rosano C., Dei Tos A.P., Doglioni C., Maestro R. Cancer - Interactions investigated in the context of cancer Dear Checker: this paper was "a pain" esp. for species/deletion/mutants - let me know if you wish to see the authors email (a very prompt reply....) -it might clarify things (??) The authors confirm that the interaction between Human p53 (and mutants) and full length Twist1 were confirmed for Human, Rat and Mouse Twist1. However Twist1 mutants are of Murine origin and P300 and Twist2 are of Human origin. The authors confirm that the interaction between Human p53 (and mutants) and full length Twist1 were confirmed for Human, Rat and Mouse Twist1. However Twist1 mutants are of Murine origin and P300 and Twist2 are of Human origin. maestro@cro.it, spiccinin@cro.it Accepted 2012-OCT-10 by PPORRAS 15-Nov-2012: Contacted by pporras. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation A "Twist box" Code of p53 Inactivation: Twist box:p53 Interaction Promotes p53 Degradation. A "Twist box" Code of p53 Inactivation: Twist box:p53 Interaction Promotes p53 Degradation. Cancer Cell (1535-6108) 2012 imex curation Piccinin S., Tonin E., Sessa S., Demontis S., Rossi S., Pecciarini L., Zanatta L., Pivetta F., Grizzo A., Sonego M., Rosano C., Dei Tos A.P., Doglioni C., Maestro R. Cancer - Interactions investigated in the context of cancer Dear Checker: this paper was "a pain" esp. for species/deletion/mutants - let me know if you wish to see the authors email (a very prompt reply....) -it might clarify things (??) The authors confirm that the interaction between Human p53 (and mutants) and full length Twist1 were confirmed for Human, Rat and Mouse Twist1. However Twist1 mutants are of Murine origin and P300 and Twist2 are of Human origin. maestro@cro.it, spiccinin@cro.it 15-Nov-2012: Contacted by pporras. Only protein-protein interactions human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Piccinin S., Tonin E., Sessa S., Demontis S., Rossi S., Pecciarini L., Zanatta L., Pivetta F., Grizzo A., Sonego M., Rosano C., Dei Tos A.P., Doglioni C., Maestro R. Cancer - Interactions investigated in the context of cancer Dear Checker: this paper was "a pain" esp. for species/deletion/mutants - let me know if you wish to see the authors email (a very prompt reply....) -it might clarify things (??) Adenovirus-E1A/Ras transformed MEFS. The authors confirm that the interaction between Human p53 (and mutants) and full length Twist1 were confirmed for Human, Rat and Mouse Twist1. However Twist1 mutants are of Murine origin and P300 and Twist2 are of Human origin. maestro@cro.it, spiccinin@cro.it Accepted 2012-OCT-10 by PPORRAS 15-Nov-2012: Contacted by pporras. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation A "Twist box" Code of p53 Inactivation: Twist box:p53 Interaction Promotes p53 Degradation. A "Twist box" Code of p53 Inactivation: Twist box:p53 Interaction Promotes p53 Degradation. Cancer Cell (1535-6108) 2012 imex curation Piccinin S., Tonin E., Sessa S., Demontis S., Rossi S., Pecciarini L., Zanatta L., Pivetta F., Grizzo A., Sonego M., Rosano C., Dei Tos A.P., Doglioni C., Maestro R. Cancer - Interactions investigated in the context of cancer Dear Checker: this paper was "a pain" esp. for species/deletion/mutants - let me know if you wish to see the authors email (a very prompt reply....) -it might clarify things (??) The authors confirm that the interaction between Human p53 (and mutants) and full length Twist1 were confirmed for Human, Rat and Mouse Twist1. However Twist1 mutants are of Murine origin and P300 and Twist2 are of Human origin. maestro@cro.it, spiccinin@cro.it 15-Nov-2012: Contacted by pporras. Only protein-protein interactions mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Piccinin S., Tonin E., Sessa S., Demontis S., Rossi S., Pecciarini L., Zanatta L., Pivetta F., Grizzo A., Sonego M., Rosano C., Dei Tos A.P., Doglioni C., Maestro R. Cancer - Interactions investigated in the context of cancer Dear Checker: this paper was "a pain" esp. for species/deletion/mutants - let me know if you wish to see the authors email (a very prompt reply....) -it might clarify things (??) Adenovirus-E1A/Ras transformed MEFS. The authors confirm that the interaction between Human p53 (and mutants) and full length Twist1 were confirmed for Human, Rat and Mouse Twist1. However Twist1 mutants are of Murine origin and P300 and Twist2 are of Human origin. maestro@cro.it, spiccinin@cro.it Accepted 2012-OCT-10 by PPORRAS 15-Nov-2012: Contacted by pporras. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation A "Twist box" Code of p53 Inactivation: Twist box:p53 Interaction Promotes p53 Degradation. A "Twist box" Code of p53 Inactivation: Twist box:p53 Interaction Promotes p53 Degradation. Cancer Cell (1535-6108) 2012 imex curation Piccinin S., Tonin E., Sessa S., Demontis S., Rossi S., Pecciarini L., Zanatta L., Pivetta F., Grizzo A., Sonego M., Rosano C., Dei Tos A.P., Doglioni C., Maestro R. Cancer - Interactions investigated in the context of cancer Dear Checker: this paper was "a pain" esp. for species/deletion/mutants - let me know if you wish to see the authors email (a very prompt reply....) -it might clarify things (??) The authors confirm that the interaction between Human p53 (and mutants) and full length Twist1 were confirmed for Human, Rat and Mouse Twist1. However Twist1 mutants are of Murine origin and P300 and Twist2 are of Human origin. maestro@cro.it, spiccinin@cro.it 15-Nov-2012: Contacted by pporras. Only protein-protein interactions human-sk-ut-1 SK-UT-1_Human uterus leiomyosarcoma (GIII) cell line sk-ut-1 SK-UT-1 Human uterus leiomyosarcoma (GIII) cell line https://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=HTB-114&Template=cellBiology Has nonsense mutation in p53 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Piccinin S., Tonin E., Sessa S., Demontis S., Rossi S., Pecciarini L., Zanatta L., Pivetta F., Grizzo A., Sonego M., Rosano C., Dei Tos A.P., Doglioni C., Maestro R. Cancer - Interactions investigated in the context of cancer Dear Checker: this paper was "a pain" esp. for species/deletion/mutants - let me know if you wish to see the authors email (a very prompt reply....) -it might clarify things (??) The authors confirm that the interaction between Human p53 (and mutants) and full length Twist1 were confirmed for Human, Rat and Mouse Twist1. However Twist1 mutants are of Murine origin and P300 and Twist2 are of Human origin. maestro@cro.it, spiccinin@cro.it Accepted 2012-OCT-10 by PPORRAS 15-Nov-2012: Contacted by pporras. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation A "Twist box" Code of p53 Inactivation: Twist box:p53 Interaction Promotes p53 Degradation. A "Twist box" Code of p53 Inactivation: Twist box:p53 Interaction Promotes p53 Degradation. Cancer Cell (1535-6108) 2012 imex curation Piccinin S., Tonin E., Sessa S., Demontis S., Rossi S., Pecciarini L., Zanatta L., Pivetta F., Grizzo A., Sonego M., Rosano C., Dei Tos A.P., Doglioni C., Maestro R. Cancer - Interactions investigated in the context of cancer Dear Checker: this paper was "a pain" esp. for species/deletion/mutants - let me know if you wish to see the authors email (a very prompt reply....) -it might clarify things (??) The authors confirm that the interaction between Human p53 (and mutants) and full length Twist1 were confirmed for Human, Rat and Mouse Twist1. However Twist1 mutants are of Murine origin and P300 and Twist2 are of Human origin. maestro@cro.it, spiccinin@cro.it 15-Nov-2012: Contacted by pporras. Only protein-protein interactions human-ht1080 Homo sapiens HT1080 fribrosarcoma cell line ht1080 confirmed as human with IEF of AST, LDH, NP established from the biopsy from a fibrosarcoma of a 35-year-old Caucasian man in 1972 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Piccinin S., Tonin E., Sessa S., Demontis S., Rossi S., Pecciarini L., Zanatta L., Pivetta F., Grizzo A., Sonego M., Rosano C., Dei Tos A.P., Doglioni C., Maestro R. Cancer - Interactions investigated in the context of cancer Dear Checker: this paper was "a pain" esp. for species/deletion/mutants - let me know if you wish to see the authors email (a very prompt reply....) -it might clarify things (??) The authors confirm that the interaction between Human p53 (and mutants) and full length Twist1 were confirmed for Human, Rat and Mouse Twist1. However Twist1 mutants are of Murine origin and P300 and Twist2 are of Human origin. maestro@cro.it, spiccinin@cro.it Accepted 2012-OCT-10 by PPORRAS 15-Nov-2012: Contacted by pporras. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation A "Twist box" Code of p53 Inactivation: Twist box:p53 Interaction Promotes p53 Degradation. A "Twist box" Code of p53 Inactivation: Twist box:p53 Interaction Promotes p53 Degradation. Cancer Cell (1535-6108) 2012 imex curation Piccinin S., Tonin E., Sessa S., Demontis S., Rossi S., Pecciarini L., Zanatta L., Pivetta F., Grizzo A., Sonego M., Rosano C., Dei Tos A.P., Doglioni C., Maestro R. Cancer - Interactions investigated in the context of cancer Dear Checker: this paper was "a pain" esp. for species/deletion/mutants - let me know if you wish to see the authors email (a very prompt reply....) -it might clarify things (??) The authors confirm that the interaction between Human p53 (and mutants) and full length Twist1 were confirmed for Human, Rat and Mouse Twist1. However Twist1 mutants are of Murine origin and P300 and Twist2 are of Human origin. maestro@cro.it, spiccinin@cro.it 15-Nov-2012: Contacted by pporras. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Piccinin S., Tonin E., Sessa S., Demontis S., Rossi S., Pecciarini L., Zanatta L., Pivetta F., Grizzo A., Sonego M., Rosano C., Dei Tos A.P., Doglioni C., Maestro R. Cancer - Interactions investigated in the context of cancer Dear Checker: this paper was "a pain" esp. for species/deletion/mutants - let me know if you wish to see the authors email (a very prompt reply....) -it might clarify things (??) The authors confirm that the interaction between Human p53 (and mutants) and full length Twist1 were confirmed for Human, Rat and Mouse Twist1. However Twist1 mutants are of Murine origin and P300 and Twist2 are of Human origin. maestro@cro.it, spiccinin@cro.it Accepted 2012-OCT-10 by PPORRAS 15-Nov-2012: Contacted by pporras. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation A "Twist box" Code of p53 Inactivation: Twist box:p53 Interaction Promotes p53 Degradation. A "Twist box" Code of p53 Inactivation: Twist box:p53 Interaction Promotes p53 Degradation. Cancer Cell (1535-6108) 2012 imex curation Piccinin S., Tonin E., Sessa S., Demontis S., Rossi S., Pecciarini L., Zanatta L., Pivetta F., Grizzo A., Sonego M., Rosano C., Dei Tos A.P., Doglioni C., Maestro R. Cancer - Interactions investigated in the context of cancer Dear Checker: this paper was "a pain" esp. for species/deletion/mutants - let me know if you wish to see the authors email (a very prompt reply....) -it might clarify things (??) The authors confirm that the interaction between Human p53 (and mutants) and full length Twist1 were confirmed for Human, Rat and Mouse Twist1. However Twist1 mutants are of Murine origin and P300 and Twist2 are of Human origin. maestro@cro.it, spiccinin@cro.it 15-Nov-2012: Contacted by pporras. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay western blot western blot Immuno blot Immuno blot Piccinin S., Tonin E., Sessa S., Demontis S., Rossi S., Pecciarini L., Zanatta L., Pivetta F., Grizzo A., Sonego M., Rosano C., Dei Tos A.P., Doglioni C., Maestro R. Cancer - Interactions investigated in the context of cancer Dear Checker: this paper was "a pain" esp. for species/deletion/mutants - let me know if you wish to see the authors email (a very prompt reply....) -it might clarify things (??) The authors confirm that the interaction between Human p53 (and mutants) and full length Twist1 were confirmed for Human, Rat and Mouse Twist1. However Twist1 mutants are of Murine origin and P300 and Twist2 are of Human origin. maestro@cro.it, spiccinin@cro.it Accepted 2012-OCT-10 by PPORRAS 15-Nov-2012: Contacted by pporras. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation A "Twist box" Code of p53 Inactivation: Twist box:p53 Interaction Promotes p53 Degradation. A "Twist box" Code of p53 Inactivation: Twist box:p53 Interaction Promotes p53 Degradation. Cancer Cell (1535-6108) 2012 imex curation Piccinin S., Tonin E., Sessa S., Demontis S., Rossi S., Pecciarini L., Zanatta L., Pivetta F., Grizzo A., Sonego M., Rosano C., Dei Tos A.P., Doglioni C., Maestro R. Cancer - Interactions investigated in the context of cancer Dear Checker: this paper was "a pain" esp. for species/deletion/mutants - let me know if you wish to see the authors email (a very prompt reply....) -it might clarify things (??) The authors confirm that the interaction between Human p53 (and mutants) and full length Twist1 were confirmed for Human, Rat and Mouse Twist1. However Twist1 mutants are of Murine origin and P300 and Twist2 are of Human origin. maestro@cro.it, spiccinin@cro.it 15-Nov-2012: Contacted by pporras. Only protein-protein interactions human-saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Piccinin S., Tonin E., Sessa S., Demontis S., Rossi S., Pecciarini L., Zanatta L., Pivetta F., Grizzo A., Sonego M., Rosano C., Dei Tos A.P., Doglioni C., Maestro R. Cancer - Interactions investigated in the context of cancer Dear Checker: this paper was "a pain" esp. for species/deletion/mutants - let me know if you wish to see the authors email (a very prompt reply....) -it might clarify things (??) The authors confirm that the interaction between Human p53 (and mutants) and full length Twist1 were confirmed for Human, Rat and Mouse Twist1. However Twist1 mutants are of Murine origin and P300 and Twist2 are of Human origin. maestro@cro.it, spiccinin@cro.it Accepted 2012-OCT-10 by PPORRAS 15-Nov-2012: Contacted by pporras. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation mdm2_human E3 ubiquitin-protein ligase Mdm2 MDM2 p53-binding protein Mdm2 Oncoprotein Mdm2 Double minute 2 protein RING-type E3 ubiquitin transferase Mdm2 protein protein human Homo sapiens Human MCNTNMSVPTDGAVTTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVLFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQQESSDSGTSVSENRCHLEGGSDQKDLVQELQEEKPSSSHLVSRPSTSSRRRAISETEENSDELSGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYQVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPSHCNRCWALRENWLPEDKGKDKGEISEKAKLENSTQAEEGFDVPDCKKTIVNDSRESCVEENDDKITQASQSQESEDYSQPSTSSSIIYSSQEDVKEFEREETQDKEESVESSLPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQPIQMIVLTYFP DIP protein Q00987 original sequence version: 93 DIP protein Q00987-1 original sequence version: 192 DIP protein Q00987 original sequence version: 88 DIP protein Q00987 original sequence version: 180 DIP protein Q00987 original sequence version: 188 DIP protein Q00987 original sequence version: 189 DIP protein Q00987 original sequence version: 115 DIP protein Q00987-1 original sequence version: null DIP protein Q00987 original sequence version: 149 DIP protein Q00987 original sequence version: 146 DIP protein Q00987 original sequence version: 140 DIP protein Q00987 original sequence version: 183 DIP protein Q00987 original sequence version: 145 DIP protein Q00987 original sequence version: 182 DIP protein Q00987 original sequence version: 151 DIP protein Q00987 original sequence version: 156 DIP protein Q00987-1 original sequence version: 160 DIP protein Q00987 original sequence version: 160 DIP protein Q00987 original sequence version: 168 DIP protein Q00987 original sequence version: 186 DIP protein Q00987 original sequence version: 206 F37CE163876BC983 cite1_human Cbp/p300-interacting transactivator 1 CITED1 MSG1 Melanocyte-specific protein 1 protein protein human Homo sapiens Human MPTTSRPALDVKGGTSPAKEDANQEMSSVAYSNLAVKDRKAVAILHYPGVASNGTKASGAPTSSSGSPIGSPTTTPPTKPPSFNLHPAPHLLASMHLQKLNSQYQGMAAATPGQPGEAGPLQNWDFGAQAGGAESLSPSAGAQSPAIIDSDPVDEEVLMSLVVELGLDRANELPELWLGQNEFDFTADFPSSC 9B0CF50CA639ECCB twst2_human Twist-related protein 2 Dermis-expressed protein 1 TWIST2 DERMO1 BHLHA39 Class A basic helix-loop-helix protein 39 protein protein human Homo sapiens Human MEEGSSSPVSPVDSLGTSEEELERQPKRFGRKRRYSKKSSEDGSPTPGKRGKKGSPSAQSFEELQSQRILANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLKLAARYIDFLYQVLQSDEMDNKMTSCSYVAHERLSYAFSVWRMEGAWSMSASH 8F44750916940C0A twst1_human Twist-related protein 1 H-twist TWIST1 TWIST BHLHA38 Class A basic helix-loop-helix protein 38 protein protein human Homo sapiens Human MMQDVSSSPVSPADDSLSNSEEEPDRQQPPSGKRGGRKRRSSRRSAGGGAGPGGAAGGGVGGGDEPGSPAQGKRGKKSAGCGGGGGAGGGGGSSSGGGSPQSYEELQTQRVMANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLKLAARYIDFLYQVLQSDELDSKMASCSYVAHERLSYAFSVWRMEGAWSMSASH DIP protein Q15672 original sequence version: 156 DIP protein Q15672 original sequence version: 159 9394E4351BA1D081 p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD DIP protein P04637 original sequence version: 121 DIP protein P04637 original sequence version: 115 DIP protein P04637-1 original sequence version: 224 DIP protein P04637 original sequence version: 117 DIP protein P04637-1 original sequence version: 226 DIP protein P04637 original sequence version: 226 DIP protein P04637 original sequence version: 212 DIP protein P04637 original sequence version: 220 DIP protein P04637-1 original sequence version: 220 DIP protein P04637-1 original sequence version: 145 DIP protein P04637 original sequence version: 146 DIP protein P04637 original sequence version: 145 DIP protein P04637 original sequence version: 166 DIP protein P04637-1 original sequence version: 166 DIP protein P04637 original sequence version: 169 DIP protein P04637 original sequence version: 177 DIP protein P04637 original sequence version: 175 DIP protein P04637-1 original sequence version: 175 DIP protein P04637-1 original sequence version: 172 DIP protein P04637-1 original sequence version: 169 DIP protein P04637 original sequence version: 170 DIP protein P04637 original sequence version: 172 DIP protein P04637-1 original sequence version: 194 DIP protein P04637 original sequence version: 196 DIP protein P04637 original sequence version: 185 DIP protein P04637-1 original sequence version: 191 DIP protein P04637 original sequence version: 191 DIP protein P04637 original sequence version: 180 DIP protein P04637-1 original sequence version: 197 DIP protein P04637 original sequence version: 183 DIP protein P04637-1 original sequence version: 183 DIP protein P04637-1 original sequence version: 185 DIP protein P04637 original sequence version: 199 DIP protein P04637 original sequence version: 201 DIP protein P04637 original sequence version: 189 DIP protein P04637 original sequence version: 235 DIP protein P04637 original sequence version: 230 DIP protein P04637 original sequence version: 234 DIP protein P04637-1 original sequence version: 234 DIP protein P04637-1 original sequence version: 235 DIP protein P04637 original sequence version: 239 DIP protein P04637-1 original sequence version: 212 DIP protein P04637-1 original sequence version: 213 DIP protein P04637 original sequence version: 213 DIP protein P04637 original sequence version: 206 DIP protein P04637-1 original sequence version: 188 DIP protein P04637-1 original sequence version: 228 DIP protein P04637 original sequence version: 208 DIP protein P04637 original sequence version: 240 DIP protein P04637-1 original sequence version: 240 DIP protein P04637 original sequence version: 204 DIP protein P04637-1 original sequence version: 204 DIP protein P04637-1 original sequence version: 209 DIP protein P04637-1 original sequence version: 203 DIP protein P04637 original sequence version: 221 DIP protein P04637-1 original sequence version: 152 DIP protein P04637 original sequence version: 217 DIP protein P04637-1 original sequence version: 140 DIP protein P04637 original sequence version: 215 DIP protein P04637 original sequence version: 214 DIP protein P04637-1 original sequence version: 182 DIP protein P04637 original sequence version: 182 DIP protein P04637 original sequence version: 188 DIP protein P04637 original sequence version: 192 DIP protein P04637-1 original sequence version: 192 DIP protein P04637 original sequence version: 218 DIP protein P04637-1 original sequence version: 218 AD5C149FD8106131 p53_mouse Cellular tumor antigen p53 Tp53 P53 Trp53 Tumor suppressor p53 protein protein mouse Mus musculus Mouse MTAMEESQSDISLELPLSQETFSGLWKLLPPEDILPSPHCMDDLLLPQDVEEFFEGPSEALRVSGAPAAQDPVTETPGPVAPAPATPWPLSSFVPSQKTYQGNYGFHLGFLQSGTAKSVMCTYSPPLNKLFCQLAKTCPVQLWVSATPPAGSRVRAMAIYKKSQHMTEVVRRCPHHERCSDGDGLAPPQHLIRVEGNLYPEYLEDRQTFRHSVVVPYEPPEAGSEYTTIHYKYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRDSFEVRVCACPGRDRRTEEENFRKKEVLCPELPPGSAKRALPTCTSASPPQKKKPLDGEYFTLKIRGRKRFEMFRELNEALELKDAHATEESGDSRAHSSYLKTKKGQSTSRHKKTMVKKVGPDSD DIP protein P02340 original sequence version: 117 DIP protein P02340 original sequence version: 165 DIP protein P02340 original sequence version: 187 DIP protein P02340 original sequence version: 201 DIP protein P02340 original sequence version: 111 DIP protein P02340 original sequence version: 141 DIP protein P02340 original sequence version: 180 DIP protein P02340 original sequence version: 163 DIP protein P02340 original sequence version: 152 ED5A607378D921D7 twst1_mouse Twist-related protein 1 Twist1 Twist M-twist protein protein mouse Mus musculus Mouse MMQDVSSSPVSPADDSLSNSEEEPDRQQPASGKRGARKRRSSRRSAGGSAGPGGATGGGIGGGDEPGSPAQGKRGKKSAGGGGGGGAGGGGGGGGGSSSGGGSPQSYEELQTQRVMANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLKLAARYIDFLYQVLQSDELDSKMASCSYVAHERLSYAFSVWRMEGAWSMSASH DIP protein P26687 original sequence version: 131 618AD8E9BE87C555 csk21_human Casein kinase II subunit alpha CSNK2A1 CK2A1 protein protein human Homo sapiens Human MSGPVPSRARVYTDVNTHRPREYWDYESHVVEWGNQDDYQLVRKLGRGKYSEVFEAINITNNEKVVVKILKPVKKKKIKREIKILENLRGGPNIITLADIVKDPVSRTPALVFEHVNNTDFKQLYQTLTDYDIRFYMYEILKALDYCHSMGIMHRDVKPHNVMIDHEHRKLRLIDWGLAEFYHPGQEYNVRVASRYFKGPELLVDYQMYDYSLDMWSLGCMLASMIFRKEPFFHGHDNYDQLVRIAKVLGTEDLYDYIDKYNIELDPRFNDILGRHSRKRWERFVHSENQHLVSPEALDFLDKLLRYDHQSRLTAREAMEHPYFYTVVKDQARMGSSSMPGGSTPVSSANMMSGISSVPTPSPLGPLAGSPVIAAANPLGMPVPAAAGAQQ DIP protein P68400 original sequence version: 88 DIP protein P68400 original sequence version: 78 DIP protein P68400 original sequence version: 100 DIP protein P68400 original sequence version: 110 DIP protein P68400 original sequence version: 125 DIP protein P68400 original sequence version: 136 D3B6F5D13FF7422D twist1-tp53-1 5 12 unspecified role unspecified role bait bait 13 unspecified role unspecified role prey prey physical association physical association 4A twist1-tp53-2 4 13 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait physical association physical association 4A twist1-tp53-3 3 14 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait physical association physical association 4A twist1-tp53-4 1 15 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined twist_box required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 13 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c_terminal_region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain in vitro In vitro physical association physical association 4B/E, S4D tp53-twist1-9 6 13 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction 4B tp53-mdm2 8 13 unspecified role unspecified role bait bait P04637:p.Ser392Glu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain S E Wed May 02 19:02:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser392glu' Tue Jun 30 16:56:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser392Glu' P04637:p.Ser392Ala mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain S A Wed May 02 17:58:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser392ala' Tue Jun 30 18:49:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser392Ala' 9 unspecified role unspecified role prey prey physical association physical association 6D tp53-mdm2-5 5 9 unspecified role unspecified role prey prey 13 unspecified role unspecified role bait bait physical association physical association 6E, S5I. The increase in the overall levels of p53 detected after Twist1 knockdown correlated with a slight but consistent decrease (∼30%) in the amount of p53 complexed to MDM2. twist1-cited1 1 10 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 15 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association S3I tp53-twist1-6 2 15 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 13 unspecified role unspecified role bait bait physical association physical association S4B tp53-twist2 1 13 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association S4C tp53-twist1-7 1 15 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P26687:p.Arg186Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain R A Wed May 02 19:24:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg186ala' Tue Jun 30 17:21:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg186Ala' P26687:p.His184Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain H A Wed May 02 16:15:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his184ala' Tue Jun 30 19:28:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His184Ala' P26687:p.Ser188Leu mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain S L Wed May 02 16:30:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser188leu' Tue Jun 30 18:03:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser188Leu' twist_box required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain in vitro In vitro 13 unspecified role unspecified role bait bait c_terminal_region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association 5C, S4A tp53-twist1-8 1 15 unspecified role unspecified role prey prey twist_box required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 13 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association 4C csnk2a1-tp53-1 7 15 inhibitor inhibitor neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 13 enzyme target enzyme target substrate substrate neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 16 enzyme enzyme neutral component neutral component ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation S5C Phosphorylation at Ser392 was attenuated in the presence of recombinant Twist1.