IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Tumor type-dependent function of the par3 polarity protein in skin tumorigenesis. Tumor type-dependent function of the par3 polarity protein in skin tumorigenesis. Cancer Cell (1535-6108) 2012 imex curation Iden S., van Riel W.E., Schafer R., Song J.Y., Hirose T., Ohno S., Collard J.G. Cancer - Interactions investigated in the context of cancer This paper shows lots of co-localisations of (H?) RAS-PAR3 but no other evidence they are actually interacting - so not entered. The authors confirm that the molecule "aPKC" as used in Figure 3 is "Protein kinase C zeta type" - UniProt ID= Q02956. sandra.iden@uk-koeln.de, j.collard@nki.nl 11-Oct-2012: Contacted by IntAct-Help. Only protein-protein interactions mouse-keratinocyte Mouse Keratinocyte keratinocyte Keratinocyte anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Iden S., van Riel W.E., Schafer R., Song J.Y., Hirose T., Ohno S., Collard J.G. Cancer - Interactions investigated in the context of cancer This paper shows lots of co-localisations of (H?) RAS-PAR3 but no other evidence they are actually interacting - so not entered. The authors confirm that the molecule "aPKC" as used in Figure 3 is "Protein kinase C zeta type" - UniProt ID= Q02956. sandra.iden@uk-koeln.de, j.collard@nki.nl Accepted 2012-SEP-20 by ORCHARD 11-Oct-2012: Contacted by IntAct-Help. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation Tumor type-dependent function of the par3 polarity protein in skin tumorigenesis. Tumor type-dependent function of the par3 polarity protein in skin tumorigenesis. Cancer Cell (1535-6108) 2012 imex curation Iden S., van Riel W.E., Schafer R., Song J.Y., Hirose T., Ohno S., Collard J.G. Cancer - Interactions investigated in the context of cancer This paper shows lots of co-localisations of (H?) RAS-PAR3 but no other evidence they are actually interacting - so not entered. The authors confirm that the molecule "aPKC" as used in Figure 3 is "Protein kinase C zeta type" - UniProt ID= Q02956. sandra.iden@uk-koeln.de, j.collard@nki.nl 11-Oct-2012: Contacted by IntAct-Help. Only protein-protein interactions mouse-keratinocyte Mouse Keratinocyte keratinocyte Keratinocyte fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Iden S., van Riel W.E., Schafer R., Song J.Y., Hirose T., Ohno S., Collard J.G. Cancer - Interactions investigated in the context of cancer This paper shows lots of co-localisations of (H?) RAS-PAR3 but no other evidence they are actually interacting - so not entered. The authors confirm that the molecule "aPKC" as used in Figure 3 is "Protein kinase C zeta type" - UniProt ID= Q02956. sandra.iden@uk-koeln.de, j.collard@nki.nl Accepted 2012-SEP-20 by ORCHARD 11-Oct-2012: Contacted by IntAct-Help. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation kpcz_mouse Protein kinase C zeta type nPKC-zeta Prkcz Pkcz protein protein mouse Mus musculus Mouse MPSRTDPKMDRSGGRVRLKAHYGGDILITSVDAMTTFKDLCEEVRDMCGLHQQHPLTLKWVDSEGDPCTVSSQMELEEAFRLVCQGRDEVLIIHVFPSIPEQPGMPCPGEDKSIYRRGARRWRKLYRANGHLFQAKRFNRGAYCGQCSERIWGLSRQGYRCINCKLLVHKRCHVLVPLTCRRHMDSVMPSQEPPVDDKNDGVDLPSEETDGIAYISSSRKHDNIKDDSEDLKPVIDGVDGIKISQGLGLQDFDLIRVIGRGSYAKVLLVRLKKNDQIYAMKVVKKELVHDDEDIDWVQTEKHVFEQASSNPFLVGLHSCFQTTSRLFLVIEYVNGGDLMFHMQRQRKLPEEHARFYAAEICIALNFLHERGIIYRDLKLDNVLLDADGHIKLTDYGMCKEGLGPGDTTSTFCGTPNYIAPEILRGEEYGFSVDWWALGVLMFEMMAGRSPFDIITDNPDMNTEDYLFQVILEKPIRIPRFLSVKASHVLKGFLNKDPKERLGCRPQTGFSDIKSHAFFRSIDWDLLEKKQTLPPFQPQITDDYGLDNFDTQFTSEPVQLTPDDEDVIKRIDQSEFEGFEYINPLLLSAEESV 690AD891C25BC311 pard3_mouse Partitioning defective 3 homolog Pard3 Par3 Atypical PKC isotype-specific-interacting protein Ephrin-interacting protein protein protein mouse Mus musculus Mouse MKVTVCFGRTRVVVPCGDGRMKVFSLIQQAVTRYRKAVAKDPNYWIQVHRLEHGDGGILDLDDILCDVADDKDRLVAVFDEQDPHHGGDGTSASFTGTQSPEIFGSELGTNNVSAFQPYQATSEIEVTPSVLRANMPLHVRRSSDPALTGLSTSVSDNNFSSEEPSRKNPTRWSTTAGFLKQNTAGSPKTCDRKKDENYRSLPRDPSSWSNQFQRDNARSSLSASHPMVDRWLEKQEQDEEGTEEDSSRVEPVGHADTGLENMPNFSLDDMVKLVQVPNDGGPLGIHVVPFSARGGRTLGLLVKRLEKGGKAEQENLFHENDCIVRINDGDLRNRRFEQAQHMFRQAMRARVIWFHVVPAANKEQYEQLSQREKNNYSPGRFSPDSHCVANRSVANNAPQALPRAPRLSQPPEQLDAHPRLPHSAHASTKPPAAPALAPPSVLSTNVGSVYNTKKVGKRLNIQLKKGTEGLGFSITSRDVTIGGSAPIYVKNILPRGAAIQDGRLKAGDRLIEVNGVDLAGKSQEEVVSLLRSTKMEGTVSLLVFRQEEAFHPREMNAEPSQMQTPKETKAEDEDVVLTPDGTREFLTFEVPLNDSGSAGLGVSVKGNRSKENHADLGIFVKSIINGGAASKDGRLRVNDQLIAVNGESLLGKANQEAMETLRRSMSTEGNKRGMIQLIVARRISRCNELRSPGSPAAPELPIETELDDRERRISHSLYSGIEGLDESPTRNAALSRIMGKCQLSPTVNMPHDDTVMIEDDRLPVLPPHLSDQSSSSSHDDVGFIMTEAGTWAKATISDSADCSLSPDVDPVLAFQREGFGRQSMSEKRTKQFSDASQLDFVKTRKSKSMDLVADETKLNTVDDQRAGSPSRDVGPSLGLKKSSSLESLQTAVAEVTLNGNIPFHRPRPRIIRGRGCNESFRAAIDKSYDKPMVDDDDEGMETLEEDTEESSRSGRESVSTSSDQPSYSLERQMNGDPEKRDKTERKKDKAGKDKKKDREKEKDKLKAKKGMLKGLGDMFRFGKHRKDDKMEKMGRIKIQDSFTSEEDRVRMKEEQERIQAKTREFRERQARERDYAEIQDFHRTFGCDDELLYGGMSSYEGCLALNARPQSPREGHLMDTLYAQVKKPRSSKPGDSNRSTPSNHDRIQRLRQEFQQAKQDEDVEDRRRTYSFEQSWSSSRPASQSGRHSVSVEVQVQRQRQEERESFQQAQRQYSSLPRQSRKNASSISQDSWEQNYAPGEGFQSAKENPRYSSYQGSRNGYLGGHGFNARVMLETQELLRQEQRRKEQQLKKQPPADGVRGPFRQDVPPSPSQVARLNRLQTPEKGRPFYS 1DD680EE824C3837 pard3-prkcz 2 4 unspecified role unspecified role neutral component neutral component 3 unspecified role unspecified role neutral component neutral component colocalization colocalization 3B aPKC was strikingly mislocalized to the cytoplasm in Par3-deficient epidermis and tumors. pard3-prkcz-1 1 3 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association 3C