IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The permeability of reconstituted nuclear pores provides direct evidence for the selective phase model. The permeability of reconstituted nuclear pores provides direct evidence for the selective phase model. Cell (0092-8674) 2012 imex curation Hulsmann B.B., Labokha A.A., Gorlich D. goerlich@mpibpc.mpg.de 15-Nov-2012: Contacted by pporras. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Hulsmann B.B., Labokha A.A., Gorlich D. goerlich@mpibpc.mpg.de Accepted 2012-NOV-05 by MGT 15-Nov-2012: Contacted by pporras. Cell (0092-8674) 2012 Only protein-protein interactions imex curation The permeability of reconstituted nuclear pores provides direct evidence for the selective phase model. The permeability of reconstituted nuclear pores provides direct evidence for the selective phase model. Cell (0092-8674) 2012 imex curation Hulsmann B.B., Labokha A.A., Gorlich D. goerlich@mpibpc.mpg.de 15-Nov-2012: Contacted by pporras. Only protein-protein interactions xenla-oocyte Xenopus laevis oocyte Oocyte anti bait coip anti bait coimmunoprecipitation anti bait coip ms participant Identification by mass spectrometry ms participant Hulsmann B.B., Labokha A.A., Gorlich D. goerlich@mpibpc.mpg.de Accepted 2012-NOV-05 by MGT 15-Nov-2012: Contacted by pporras. Cell (0092-8674) 2012 Only protein-protein interactions imex curation q6dcp4_xenla Nup98 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MFNKTFGSPFGTGNGAFGATSTFGQTTGFGTTPAAAFGSAGFGTNTTTGGLFGNTQTKPGSLFGANTFNQPATSSASSGFGFGASTGTTNSLFGSTNTGSSLFSTPSNAFAQTKPASFGNFGTSTSTGGLFGNTNTANPFSGTSASLFGASTFSSAPTGTTIKFNPQSGTDTMAKGGVTTNISTKHQCITAMKEYESKSLEELRLEDYQANRKGPQNPVGAPAGTGLFGTSAATSSAPTGIFGSTAANSFSFAGNKTTFGAAGTGAFAANTGGLFGQPTNPPAASLFNKPFGNAATTQSTGFSFGNTSTLGQAQTSTMGLFGANQATQAGGLFGATTNTNAAGAFGAGSSLFGQPNPAPFGGGSTLFGNKPAGFGTTTTTAPAFGTTTGGLFGNKATLTLGTNTNTSNFGFGANAAGTSLFGNKPATGTIGPSLGTSFGTALNPGQTSLFGGNQPKLTGTLGTGAFGGTGFNSTSTGLGFGAPQPAMATLTDPNASAAQQMFLQQQYNALRYSPFPDSPLFRNPISDPKKKEELLKPTNPAAQKAVLTPTHYKLTPRPAARVRPKALQNSGAAKSQLFDALDDDEPVLSSGVFMPKKSIKKLDLKYLKNSSSLFAQESKDVNEVVPPTPETQDRSVENHHADEKKKK BD50CEE0732297BC q642r6_xenla nup205.S MGC83295 nup205 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MAAQLALNSEASLWGPYREIWQTVLSALIKRQPEAVHSLDIVLKKYKPDFISLFKNPPKSAQQHERVQKASTEGIPIKGTQRTRILEEQLIKEAFILSDLYNIGEIAAVELLLIGEQQQPTFHGLTRGLVAILLYWDGKSCMAESLLHLIQARKGKTFTLDHSPEVVSMVTRFTDDLMEQGLTNKILTLISQIDVNNEFDKLKKERGLGNKKHRKEVSDLIKECQQSLAHSLYSWSCQTPLNREDTLLLIGYLEKVTVEGDGSLDKVNLTLLMSLLYCLDVGFLEQGTDDREELMKQASMFMDRQYIAAIHNRLQNTQPWKSPGMQATVRLAWALALRGISQFSEVLEFSEADEPMAEIAIGGNVFLFLTEAVVGSESFCTDEFFIRRIHKLVTDFPTLMPMKVKQLRNRAEEDARLIQMSMQMGNEPPASLRRDLEHLLLLIGELYRKDPFHLELALEYWCPTEPLQSTSLMGSFLGVAHQRPPQRQVLLSKFVRQMSDLLPATLYLPYLKMLRGLASGPQCAHYCFSLLKANGGSSAENLQAAGGSPVSWDHFFHSLMLYHEHLRRDLPNTDNIHQRHPPLRGITQRELDGLIACLQLTCTIIDWSESARLALCEHAQWMPVVVILGLLQCSIPPLLKAELLKTLAAFGKSPEIAASLWQSLEYTQILQTVRATGLRQGVGIEVELNEIESRCEEYPLTRAFCQLISTLVESSFPTNLGAGLRAPGFEPYLQFLRDTVFLRYRTRAYRRAAEKWEVAEAVLDVFYKLLKDYEPQPEDFVDQYVELQGEERVAFKPPGFSLMHHLLNESPMLELCLSLMEEGVTQLDTYAPFPGKKHLEKAVAYCFMLLNLTLQKENRFMDLLRESHLSMIVTPLEQLLQGINPRSKKADNVVNIARYLCHGNSNAELAFESAKILCSISCNSKIQEKIVGDFTQDQNVSQKLMVGFVSCLDSEEAEELLDSEKEAEDQVKQTNIRYMTKIHILNLLITSLEMKAPNLAMFLLGYELKKPVSTTNLQDSGVLGCPRTCLHSILDILRKGTDVRAGPVAVWDTPHLAELCYQVIYQLCACADTSGPTMRYLRTSQDFLFSQLQHLPFSVEESEISAMNQMSWLMKTATIELRITSLNRQRSHTQRLLHLLLDDMPTRPYSADGEGGMEDESRSLSGFLHFDTTSKVRRKILRILDSIQFSNEIPEPLQLDFFDRSQIEQVIANCEHKNRRGQTVCNVKLLHRVLVAEVNALQGMAAIGQRPLLMEEINTILQYVVERNKLLQCLHAKRHALESWRQLVEIILTACPQDLIPTEHRQLIIRDLLQDLHVKILDDDAAQELMPIVAGAVFTLTAHLSQSVRTELKQPMTASGLGQSQYVQMLDGSFAAPPGTENISAGFASIGDSSLHMILRNLLEFILKTGGGFQRVRAHLYGSLLYYLQIAQRPDEPDTLESAHKSMWERLTAPEDVFSKLQRDNLSIFESYGTALMEVVCRDACDGHDIGRMLALALLDRIVSVDRQQQWLLYLSNSGYLKVLVDSLAEDDVVLRNLLTPQPPLLKALYIYESKMAFLTRVAKSSQGAIELLRSGVIVRLAQCQVYDMRPETDPHGVFGMRETPVFIPAPVERYRQILLPALQICQLILTSSTAQHLQAAGQVLQFLVAHSDTIQAILRSQEGSLGSLQELALLTGIISKAALPGVLNELDIGLNDGSMMELQGHIGRFQRQCLALLNRFGGSDRLRQLSLQDDSSRLDGVSKKDDMELAMQQICSNVMEYCQALMIQNSPSFQQTVCLFTPSLKESASRDGTRQDSQVSILPSWRLPSLGVVIHLLKQSANNFFTYYDIHRQSVGKLQNVEQLPPDEIKELCQSEMPVGADKISTTQKYGLARRRLVKLINSRAKLLSLCSYIIETCLYILWRHLEYYLLHCTTSDSQDPVFSNMTFGNRRFQDTFNTDPNMDPRNLRQNKVSQQDVDTLLREGANSFGESLQKRLLDIESLYCKVRSRHSFIQALVRRIRGLLRVSRV B66A9348A1D04EB5 q6pay1_xenla nup54.S MGC68541 nup54 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MAFNFGATTGTPANQGGFGGFGATTTASTGPAFSFTTPANTTSGLFGATQNKGFGFGTGFGSTTTSTGLGTGLGTGLGFTGFNTSQQQQQQSVLGAGLFNQSFQSTPQSNQLINTASALSAPTLLGDERDAILAKWNQLQAFWGTGKGFFMNNTPPVEFTQENPFCRFKAVGFSYIPNNKDEDGMISLIFNKKESDIRGQQQQLVESLHKVLGGHQTLTVNVEGVKTKADNQTEVIIYVVERSPNGTSRRVGASALFSYFEQAHIKANMQQLGVTGAMAQTELSPVQIKQLIQNPLSGVDPIIWEQAKVDNPDPERLIPVPMIGFKELLRRLEVQDQMTKQHQSRLDIISEDIGELQKNQTTTMAKIGQYKRKLMELSHRVLQVLIKQEIQRKSGFAIQAEEEQLRVQLDTIQSELNAPTQFKGRLNELMSQIRMQNHFGAVRSEEKYYVDADLLREIKQHLKQQQEGVSHLISIIKDNHEDIKLIEQGLNDNLHMRTGFLS 0BF7E979DD23E635 hsp70_xenla Heat shock 70 kDa protein protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MATKGVAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPQNTVFDAKRLIGRKFNDPVVQCDLKHWPFQVVSDEGKPKVKVEYKGEEKSFFPEEISSMVLTKMKETAEAYLGHPVTNAVITVPAYFNDSQRQATKDAGVLAGLNILRIINEPTAAAIAYGLDKGARGEQNVLIFDLGGGTFDVSILTIDDGIFEVKATAGDTHLGGEDFDNRMVNHFVEEFKRKHKKDIGQNKRALRRLRTACDRAKRTLSSSSQASIEIDSLFEGIDFYTAITRARFEELCSDLFRGTLEPVEKALRDAKLDKSQIHEIVLVGGSTRIPKVQKLLQDFFNGRELNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTVLIKRNTTIPTKQTQSFTTYSDNQPGVLIQVFEGERAMTKDNNLLGKFELSGIPPAPRGVPQIEVTFDIDANGILNVSAVEKSSGKQNKITITNDKGRLSKEDIEKMVQEAEKYKADDDAQRERVDAKNALESYAFNLKSMVEDENVKGKISDEDKRTISEKCTQVISWLENNQLAEKEEYAFQQKDLEKVCQPIITKLYQGGVPGGVPGGMPGSSCGAQARQGGNSGPTIEEVD 33AFA9EBBFA2FE23 nup93_xenla Nuclear pore complex protein Nup93 nup93 93 kDa nucleoporin An4a Nucleoporin Nup93 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MDGEGFGELLQQAEQLAAETEGVTELPHVERNLQEIQQAGERLRSKTMTRTSQESANVKASVLLGSRGLDISHISQRLESLSAATTFEPLEPVKDTDIQGFLKNEKDNALLSAIEESRKRTFVMAEEYHRESMLVEWEQVKQRVLHTLLASGEDALDFTQESETSYISESGAPGRSSLDNVEMAYARQMYMYNEKVVSGHLQPSLVDLCTEAAERLDDKNVSDLWVMVKQMTDVPLIPASDTLKSRCSGQMQMAFVRQALNYLEQSYKNYTLISVFANLQQAQLGGVPGTYNLVRSFLNIRLPTPIPGLQDGEIEGYPVWALIYYCMRCGDLMAAQQVVNRAQHQLGDFKNCFQEYIHNKDRRLSPTTENKLRLHYRRAVRASTDPYKRAVYCIIGRCDVSDNHSEVADKTEDYLWLKLSQVCFEDEANSSPQDRLTLPQFQKQLFEDYGESHFAVNQQPYLYFQVLFLTAQFEAAIAFLFRLERTRCHAVHVALALFELKLLLKSTGQSAQLLSQEPGEPQGVRRLNFIRLLMLYTRKFEPTDPREALQYFYFLRNEKDNQGESMFLRCVSELVIESREFDMLLGKLEKDGSRKPGAIDKFTRDTKTIINKVASVAENKGLFEEAAKLYDLAKNPDKVLELTNKLLSPVVSQISAPQSNRERLKNMALAIAERYKSQGVSAEKSINSTFYLLLDLITFFDEYHAGHIDLSFDVIERLKLVPLSQDSVEERVAAFRNFSDEIRHNLSEILLATMNILFTQYKRLKGSGPTTLGRPQRVQEDKDSVLRSQARALITFAGMIPYRMSGDTNARLVQMEVLMN DIP protein Q7ZX96 original sequence version: 53 12EDE67C51157D4A nup1_yeast Nucleoporin NUP1 NUP1 YOR098C YOR3182C Nuclear pore protein NUP1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSNTSSVMSSPRVEKRSFSSTLKSFFTNPNKKRPSSKKVFSSNLSYANHLEESDVEDTLHVNKRKRVSGTSQHSDSLTQNNNNAPIIIYGTENTERPPLLPILPIQRLRLLREKQRVRNMRELGLIQSTEFPSITSSVILGSQSKSDEGGSYLCTSSTPSPIKNGSCTRQLAGKSGEDTNVGLPILKSLKNRSNRKRFHSQSKGTVWSANFEYDLSEYDAIQKKDNKDKEGNAGGDQKTSENRNNIKSSISNGNLATGPNLTSEIEDLRADINSNRLSNPQKNLLLKGPASTVAKTAPIQESFVPNSERSGTPTLKKNIEPKKDKESIVLPTVGFDFIKDNETPSKKTSPKATSSAGAVFKSSVEMGKTDKSTKTAEAPTLSFNFSQKANKTKAVDNTVPSTTLFNFGGKSDTVTSASQPFKFGKTSEKSENHTESDAPPKSTAPIFSFGKQEENGDEGDDENEPKRKRRLPVSEDTNTKPLFDFGKTGDQKETKKGESEKDASGKPSFVFGASDKQAEGTPLFTFGKKADVTSNIDSSAQFTFGKAATAKETHTKPSETPATIVKKPTFTFGQSTSENKISEGSAKPTFSFSKSEEERKSSPISNEAAKPSFSFPGKPVDVQAPTDDKTLKPTFSFTEPAQKDSSVVSEPKKPSFTFASSKTSQPKPLFSFGKSDAAKEPPGSNTSFSFTKPPANETDKRPTPPSFTFGGSTTNNTTTTSTKPSFSFGAPESMKSTASTAAANTEKLSNGFSFTKFNHNKEKSNSPTSFFDGSASSTPIPVLGKPTDATGNTTSKSAFSFGTANTNGTNASANSTSFSFNAPATGNGTTTTSNTSGTNIAGTFNVGKPDQSIASGNTNGAGSAFGFSSSGTAATGAASNQSSFNFGNNGAGGLNPFTSATSSTNANAGLFNKPPSTNAQNVNVPSAFNFTGNNSTPGGGSVFNMNGNTNANTVFAGSNNQPHQSQTPSFNTNSSFTPSTVPNINFSGLNGGITNTATNALRPSDIFGANAASGSNSNVTNPSSIFGGAGGVPTTSFGQPQSAPNQMGMGTNNGMSMGGGVMANRKIARMRHSKR 4AC23567D2FB53CC 4AC23567D2FB53CC a4fu91_xenla nup58.L nup58 nupl1 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MASGFAFAAAAGTATLNPAAGAVAPFSFGGTPAASNTGTTGGLAFGAFGAAATPATTTAATGLGGGLFGAKPATGFTLGGANTATATTTSASTGFSIGFNKPAGSATPFSLPVTSTSSGGLSLASALTSTPATGPSPFTLNLGSTPATTTAASIGLSLGGTLTGLGGSLFQNINPSTGLGQITLGQNTLGLGLNLGAVAPVSQATTHEGLGGLDFSNSSDKKSQKAGARPEDSKALKDENLPQLLCQDVENFQKFVKQQKQVQEEISRMSSKAMLKVQEDIKALKQLLSVASSGLQRNALAIDKLKIETAQELKNAEIALRTQKTPPGLQHENTAPAYYFHTLVQQFEVQLQQYRQQIEELENHLATQSNSLHLSPQDLSMAMQKLYQTFVALAAQLQAVNENFKMLKEQYLGYRKTFLGDSTDVFEARRAEAKKWQSAPRVTTGPTPFSNIPNAAAVAMAATLTQQQQPTTGPRPSLGVSFGTPFGSAMNAGLQPSGLGSSGLGGFGSSSAFGGNTSGSSSFGFGTANKPSGSLSAGFGSTSTSGFNFSNPGINASAGLTFGVSNPSSTGFGTAGQLLQLKKPPTGNKRGKR A62D2F77D5CF1E0C imb_xenla Importin subunit beta p97 Importin-90 Karyopherin subunit beta kpnb1 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MELVTILEKTVSPDRNELEAAQKFLEQAAVENLPTFVVELSKVLANPANSQVARVAAGLQIKNPLTSRDPDVKAQYQQRWLAIDASARGEIKTYVLRTLGTESYRPSSASQCVAGIACAEITVNQWPQLIPQLVANVTDPNSTERMKESTLEAIGYICQDIDPEQLQHKSNEILTAIIQGMRKEEPSNNVRLAATNALLNSLEFTKANFDKESERHYIMQVVCEATQCPDTRVRVAALQNLVKIMSLYYQYMETYMGPALFAITVEAMKNEIDEVALQGIEFWSNVCDEEMDLAIEASEAAEQGRPPEHTSKFYAKGALQYLVPILTQTLTKQDENDDDDDWNPCKAAGVCLMLLATCCEDDIVPHVLPFIKEHIKNPDWRYRDAAVMAFGCILEGPESCQLKPLVIQAMPTLIELMKDPSVVVRDTTAWTVGRICELLPEAAINDVYLAPLLQCLIEGLGAEPRVASNVCWAFSSLAEAAYEAADVADDQEEPSSYCLSSSFEVIVQKLLETTDRPDGHQNNLRSAAYEALMEIVKNSAKDCYPAVQKTTLVIMERLQQVLQVESHIQSTSDRIQFNDLQSLLCATLQNVLRKVQHQDALQISDVVMASLLRMFQSTAGSGGVQEDALMAVSTLVEVLGAEFLKYMEAFKPFLTIGLKNYAEYQVCLAAVGLVGDLCRALQSNILPFCDEMMQFLLENLGNENVHRSVKPQILSVFGDVALAIGGEFKKYLDVVLNTLQQASQAQVDKSDYDMVDYLNELREGCIEAYTGIIQGLKGDQENVHPDVMLVQPRVEFILSFIDHIAGDEDHTDSVVACGAGLIGDLCTAFGKDVLKLVEARPMIHELLTEGRRSKTNKTKTLATWATKELRKLKNQA 2F8FAACECFFA3AD9 q91349_xenla nup62.L IL4I1 nup62 XELAEV_18038559mg protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MSGFNFGAASAGGFSFGNPKSTTTTAPTGFSFGAATAAPSGGFSFGTATPTPASTTGQTSGLFSFSNPAPSLAPTSGFSFGAQVTSTPAPSSGGLAFGANTSKLNSGVGNQPAGGTTQTSQPMGGFSFGAATTQTQPSATSVGGFSFAGGVGSTSTNVFAQPAASTGITLQSAVSTAAAPTATTSQPTSTFSFGTQPQAAPALNFGLLSSSSVLSTASTPAAAQPVAPTTGLSLNFGKPADTSAAVTSTGSTTTNTPSLSSLLGTSGPSLFSSVATSTVPSVVSTVASGLSLTSTATSTGFGMKTLASSAVPTGTLATSTASLGVKAPLAGTIVQANAVGSAAATGISTATAMTYAQLENLINKWSLELEDQEKHFLQQATQVNAWDRTLMQNGERITTLHREMEKVKLDQKRLDQELDFILSQQKELEDLLTPLEESVKEQSGTIYLQHADEEREKTYKLAENIDAQLKRMAQDLKEVIEHLNTSAGPGDASNPLQQICKILNAHMDSLQWIDQNSALLQRKVEQVTKECESRRKEQERGFSIAFD DIP protein Q91349 original sequence version: 64 BC703F1F7AEFA320 q9pvz2_xenla nup214.L CAN nup214 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MEDDTDLPPERETKDFQFRQLKKVRLFDYPADLPKQRSNLLVISNKYGLLFVGGFMGLKVFHTKDILVTVKPKENANKTVVGPQGIHVPMNSPIHHLALSSDNLTLSVCMTSAEQGSSVSFYDVRTLLNESKQNKMPFASCKLLRDPSSSVTDLQWNPTLPSMVAVCLSDGSISVLQVTDTVSVFANLPATLGVTSVCWSPKGKQLAVGKQNGTVVQYLPSLQEKKVIPCPSFYDSDNPVKVLDVLWLSTYVFTVVYAAADGSLEASPQLVIVTLPKKEDKRAERFLNFTETCYSICSERQHHFFLNYIEDWEILLAASAASVDVGVIARPPDQVGWEQWLLEDSSRAEMPMTENNDDTLPMGVALDYTCQLEVFISESQILPPVPVLLLLSTDGVLCPFHVVNLNQGVKPLTTSPEQLSLDGEREMKVVGGTAVSTPPAPLTSVSAPAPPASAAPRSAAPPPYPFGLSTASSGAPTPVLNPPASLAPAATPTKTTSQPAAAATSIFQPAGPAAGSLQPPSLPAFSFSSANNAANASAPSSFPFGAAMVSSNTAKVSAPPAMSFQPAMGTRPFSLATPVTVQAATAPGFTPTPSTVKVNLKDKFNASDTPPPATISSAAALSFTPTSKPNATVPVKSQPTVIPSQASVQPNRPFAVEAPQAPSSVSIASVQKTVRVNPPATKITPQPQRSVALENQAKVTKESDSILNGIREEIAHFQKELDDLKARTSRACFQVGSEEEKRQLRTESDGLHSFFLEIKETTESLRGEFSAMKIKNLEGFASIEDVQQRNKLKQDPKYLQLLYKKPLDPKSETQMQEIRRLNQYVKNAVQDVNDVLDLEWDQYLEEKQKKKGIIIPERETLFNSLANHQEIINQQRPKLEQLVENLQKLRLYNQISQWNVPDSSTKSFDVELENMQKTLSQTAIDTQTKPQAKLPAKISPVKQSQLRNFLSKRKTPPVRSLAPANLSRSAFLAPSFFEDLDDVSSTSSLSDMADNDNRNPPPKEIERQETPPPESTPVRVPKHAPVARTTSVQPGLGTASLPFQSGLHPATSTPVAPSQSIRVIPQGADSTMLATKTVKHGAPNITAAQKAAVAAMRRQTASQIPAASLTESTLQTVPQVVNVKELKNNGPGPTIPTVIGPTVPQSAAQVIHQVLATVGSVSARQAAPAAPLKNPPASASSIAPQTWQGSAPNKPAAQAIPKSDPSASQAPAPSVSQVNKPVSFSPAAGGFSFSNVTSAPVTSALGSSSAGCAATARDSNQASSYMFGGTGKSLGSEGSFSFASLKPASSSSSSSVVEPTMSKPSVVTAASTTATVTSTTAASSKPGEGLFQGFSGGETLGSFSGLRVGQADEASKVEVAKTPTAAQPVKLPSNPVLFSFAGAPQPAKVGEAPSTTSSTSASLFGNVQLASAGSTASAFTQSGSKPAFTFGIPQSTSTTAGASSAIPASFQSLLVSAAPATTTPSAPINSGLDVKQPIKPLSEPADSSSSQQQTLTTQSAAEQVPTVTPAATTATALPPPVPTIPSTAEAKIEGAAAPAIPASVISSQTVPFTSTVLASQTPLASTPAGGPTSQVPVLVTTAPPVTTESAQTVSLTGQPVAGSSAFAQSTVTAASTPVFGQALASGAAPSPFAQPTSSSVSTSANSSTGFGTSAFGATGGNGGFGQPSFGQAPLWKGPATSQSTLPFSQPTFGTQPAFGQPAASTATSSAGSLFGCTSSASSFSFGQASNTSGTSTSGVLFGQSSAPVFGQSAAFPQAAPAFGSASVSTTTTASFGFGQPAGFASGTSGSLFNPSQSGSTSVFGQQPASSSGGLFGAGSGGASTVGLFSGLGAKPSQEAANKNPFGSPGSSGFGSAGASNSSNLFGNSGAKAFGFGGTSFGDKPSATFSAGGSVASQGFSFNSPTKTGGFGAAPVFGSPPTFGGSPGFGGSPAFGTAAAFSNTLGSTGGKVFGEGTSAATTGGFGFGSNSSTAAFGSLATQNTPTFGSISQQSPGFGGQSSGFSGFGAGPGAAAGNTGGFGFGVSNPTSPGFGCWRS 2250EA49A0435635 nup62-kpnb1 2 10 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey association association Supp. Fig. 1B Importin-alpha and Nup88 also identified but could not be ascribed to a single gene product. Bait antibody mouse monoclonal nup62-hsp70 2 10 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey association association Supp. Fig. 1C Importin-alpha and Nup88 also identified but could not be ascribed to a single gene product. Bait antibody rabbit polyclonal nup54-nup93 2 10 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait association association Supp. Fig. 1C Importin-alpha was also identified but could not be ascribed to a single gene product. Bait antibody mouse monoclonal nup98-kpnb1 1 3 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role prey prey direct interaction direct interaction Fig. 6B Mutation of all hydrophobic residues (Phe, Leu, and Ile) of the Nup98 FG domain to serines inhibited this interaction. nup1-kpnb1 1 10 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range direct interaction direct interaction Fig. 6B Low levels of yeast NUP1 (2-175) also bound importin-beta.