MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Structure and stability of a thermostable carboxylesterase from the thermoacidophilic archaeon Sulfolobus tokodaii. Structure and stability of a thermostable carboxylesterase from the thermoacidophilic archaeon Sulfolobus tokodaii. FEBS J. (1742-464X) 2012 Angkawidjaja C., Koga Y., Takano K., Kanaya S. clement@bio.mls.eng.osaka-u.ac.jp in vitro In vitro comigration in gel comigration in gel electrophoresis comigration in gel predetermined predetermined participant predetermined Angkawidjaja C., Koga Y., Takano K., Kanaya S. clement@bio.mls.eng.osaka-u.ac.jp Urea-induced dimer dissociation was done by incubating the proteins in 10 mM Tris-HCl pH 8.0 containing 0, 1, 2, 3, 4, 5, 6, 7 or 8 M urea for 2.5 hours, followed by native PAGE containing the corresponding concentrations of urea. The gel was then stained with Coomassie Brilliant Blue and the intensities of the bands representing the dimeric, monomeric and denatured states of the samples were estimated using ImageJ FEBS J. (1742-464X) 2012 Structure and stability of a thermostable carboxylesterase from the thermoacidophilic archaeon Sulfolobus tokodaii. Structure and stability of a thermostable carboxylesterase from the thermoacidophilic archaeon Sulfolobus tokodaii. FEBS J. (1742-464X) 2012 Angkawidjaja C., Koga Y., Takano K., Kanaya S. clement@bio.mls.eng.osaka-u.ac.jp in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Angkawidjaja C., Koga Y., Takano K., Kanaya S. clement@bio.mls.eng.osaka-u.ac.jp FEBS J. (1742-464X) 2012 q976w8_sulto STK_00710 ST0071 protein protein sulto Sulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) MIDPKIKKLLESTIQLPIGKASVEEIRSLFKQFSSLTPREEVGKIEDITIPGSETNIKARVYYPKTQGPYGVLVYYHGGGFVLGDIESYDPLCRAITNSCQCVTISVDYRLAPENKFPAAVVDSFDALKWVYNNSEKFNGKYGIAVGGDSAGGNLAAVTAILSKKENIKLKYQVLIYPAVSFDLITKSLYDNGEGFFLTREHIDWFGQQYLRSFADLLDFRFSPILADLNDLPPALIITAEHDPLRDQGEAYANKLLQSGVQVTSVRFNNVIHGFVSFFPFIEQGRDAIGLIGYVLRKVFYGK mint FABA5ADB08C05714 q976w8 2 Sto-Es 3 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 4.0 direct interaction direct interaction mint t1 f2a f2c The atomic coordinates and structural factors have been deposited in the Protein Data Bank with accession numbers of 3AIK for non-inhibited Sto-Est, 3AIL for DEP-bound, 3AIM for R267E, 3AIN for R267G, and 3AIO for R267K. q976w8-1 1 Sto-Es 3 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified Q976W8:p.Arg267Gly mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R G Tue Jul 12 10:49:07 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 19:11:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg267gly' Tue Jun 30 19:43:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg267Gly' his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 2.0 direct interaction direct interaction f5a mint q976w8-1 1 Sto-Es 3 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified Q976W8:p.Arg267Glu mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R E Tue Jul 12 10:07:08 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 16:51:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg267glu' Tue Jun 30 16:12:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg267Glu' his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 2.0 direct interaction direct interaction f5a mint