MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it SIRPα interacts with nephrin at the podocyte slit diaphragm. SIRPα interacts with nephrin at the podocyte slit diaphragm. FEBS J. (1742-464X) 2012 Kajiho Y., Harita Y., Kurihara H., Horita S., Matsunaga A., Tsurumi H., Kanda S., Sugawara N., Miura K., Sekine T., Hattori S., Hattori M., Igarashi T. rat-kidney Rat kidney kidney kidney [Renal] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kajiho Y., Harita Y., Kurihara H., Horita S., Matsunaga A., Tsurumi H., Kanda S., Sugawara N., Miura K., Sekine T., Hattori S., Hattori M., Igarashi T. FEBS J. (1742-464X) 2012 SIRPα interacts with nephrin at the podocyte slit diaphragm. SIRPα interacts with nephrin at the podocyte slit diaphragm. FEBS J. (1742-464X) 2012 Kajiho Y., Harita Y., Kurihara H., Horita S., Matsunaga A., Tsurumi H., Kanda S., Sugawara N., Miura K., Sekine T., Hattori S., Hattori M., Igarashi T. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Kajiho Y., Harita Y., Kurihara H., Horita S., Matsunaga A., Tsurumi H., Kanda S., Sugawara N., Miura K., Sekine T., Hattori S., Hattori M., Igarashi T. FEBS J. (1742-464X) 2012 nphn_rat Nephrin Nphs1 Nphn Renal glomerulus-specific cell adhesion receptor protein protein rat Rattus norvegicus (Rat) Rat MGAKRVTVRGARTSPIHRMSSLTPLLLMGMLTSGLAESPVPTSAPRGFWALSENLTAVEGTTVKLWCGVRAPGSVVQWAKDGLLLGPNPKMPGFPRYSLEGDRAKGEFHLLIEACDLSDDAEYECQVGRSELGPELVSPKVILSILVSPKVLLLTPEAGSTVTWVAGQEYVVTCVSGDAKPAPDITFIQSGRTILDVSSNVNEGSEEKLCITEAEARVIPQSSDNGQLLVCEGSNPALDTPIKASFTMNILFPPGPPVIDWPGLNEGHVRAGENLELPCTARGGNPPATLQWLKNGKPVSTAWGTEHAQAVAHSVLVMTVRPEDHGARLSCQSYNSVSAGTQERSITLQVTFPPSAITILGSVSQSENKNVTLCCLTKSSRPRVLLRWWLGGRQLLPTDETVMDGLHGGHISMSNLTFLVRREDNGLPLTCEAFSDAFSKETFKKSLTLNVKYPAQKLWIEGPPEGQYIRTGTRVRLVCLAIGGNPDPSLIWFKDSRPVSEPRQPQEPRRVQLGSVEKSGSTFSRELVLIIGPPDNRAKFSCKAGQLSASTQLVVQFPPTNLTILANSSALRPGDALNLTCVSISSNPPVNLSWDKEGERLEDVAAKPQSAPFKGSAASRSVFLRVSSRDHGQRVTCRAHSEALRETVSSFYRFNVLYPPEFLGEQVRAVTVVEQGQVLLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSGGALQLWNVTRADDGFYQLHCQNSEGTAEALLKLDVHYAPTIRALRDPTEVNVGGSVDIVCTVDANPILPEMFSWERLGEEEEDLNLDDMEKVSKGSTGRLRIRQAKLSQAGAYQCIVDNGVAPAARGLVRLVVRFAPQVDQPTPLTKVAAAGDSTSSATLHCRARGVPNIDFTWTKNGVPLDLQDPRYTEHRYHQGVVHSSLLTIANVSAAQDYALFKCTATNALGSDHTNIQLVSISRPDPPLGLKVVSISPHSVGLEWKPGFDGGLPQRFQIRYEALETPGFLHVDVLPTQATTFTLTGLKPSTRYRIWLLASNALGDSGLTDKGIQVSVTTPGPDQAPEDTDHQLPTELPPGPPRLPLLPVLFAVGGLLLLSNASCVGGLLWRRRLRRLAEEISEKTEAGSEDRIRNEYEESQWTGDRDTRSSTVSTAEVDPNYYSMRDFSPQLPPTLEEVLYHQGAEGEDMAFPGHLHDEVERAYGPPGAWGPLYDEVRMDPYDLRWPEVQCEDPRGIYDQVAADMDAVEASSLPFELRGHLV mint homomint 6A3550AB907883EC shps1_rat Tyrosine-protein phosphatase non-receptor type substrate 1 Sirpa Bit Brain Ig-like molecule with tyrosine-based activation motifs CD172 antigen-like family member A Inhibitory receptor SHPS-1 Mfr Macrophage fusion receptor Macrophage membrane protein MFP150 Ptpns1 Shps1 Signal-regulatory protein alpha-1 Sirp protein protein rat Rattus norvegicus (Rat) Rat MEPAGPAPGRLGPLLFCLLLSASCFCAGASGKELKVTQADKSVSVAAGDSATLNCTVSSLTPVGPIKWFKGEGQNRSPIYSFIGGEHFPRITNVSDATKRNNMDFSICISNVTPEDAGTYYCVKFQKGIVEPDTEIKSGGGTTLYVLAKPSSPEVSGPDSRGSPGQTVNFTCKSYGFSPRNITLKWLKDGKELSHLETTISSKSNVSYNISSTVSVKLSPEDIHSRVICEVAHVTLEGRPLNGTANFSNIIRVSPTLKITQQPLTPASQVNLTCQVQKFYPKALQLNWLENGNLSRTDKPEHFTDNRDGTYNYTSLFLVNSSAHREDVVFTCQVEHDSQPAITENHTVRAFAHSSSGGSMETIPDNNAYYNWNVFIGVGVACALLVVLLMAALYLLRIKQKKAKGSTSSTRLHEPEKNAREITQIQDTNDINDITYADLNLPKEKKPAPRVPEPNNHTEYASIETGKLPRPEDTLTYADLDMVHLNRAQPTPKPEPSFSEYASVQVQRK mint homomint 5BE1FE0A4DD429F4 sirpa-nphs1 2 Sirp-alpha 4 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Nephrin 3 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell physical association physical association homomint mint f3a f3b f3c Because the SIRPα 4YF mutant, in which four cytoplasmic tyrosine residues are substituted for phenylalanines, was also co-immunoprecipitated with nephrin, SIRPα interacts with nephrin independent of its cytoplasmic phosphorylation status (Fig. 3B). This interaction was not altered by co-transfection with Fyn, or by mutation of nephrin Y1204F which disrupts its interaction with Nck or PLC-γ [14] (Fig. 3C). sirpa-nphs1-1 1 Sirp-alpha 4 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role bait bait subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level Nephrin 3 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association f3d homomint mint Glomerular endogenous nephrin was co-immunoprecipitated with endogenous SIRPα, suggesting that SIRPα forms a complex with nephrin in vivo (Fig. 3D).