MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it THAP11, a novel binding protein of PCBP1, negatively regulates CD44 alternative splicing and cell invasion in a human hepatoma cell line. THAP11, a novel binding protein of PCBP1, negatively regulates CD44 alternative splicing and cell invasion in a human hepatoma cell line. FEBS Lett. (0014-5793) 2012 Lian WX., Yin RH., Kong XZ., Zhang T., Huang XH., Zheng WW., Yang Y., Zhan YQ., Xu WX., Yu M., Ge CH., Guo JT., Li CY., Yang XM. suggested by lperfetto human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lian WX., Yin RH., Kong XZ., Zhang T., Huang XH., Zheng WW., Yang Y., Zhan YQ., Xu WX., Yu M., Ge CH., Guo JT., Li CY., Yang XM. suggested by lperfetto FEBS Lett. (0014-5793) 2012 THAP11, a novel binding protein of PCBP1, negatively regulates CD44 alternative splicing and cell invasion in a human hepatoma cell line. THAP11, a novel binding protein of PCBP1, negatively regulates CD44 alternative splicing and cell invasion in a human hepatoma cell line. FEBS Lett. (0014-5793) 2012 Lian WX., Yin RH., Kong XZ., Zhang T., Huang XH., Zheng WW., Yang Y., Zhan YQ., Xu WX., Yu M., Ge CH., Guo JT., Li CY., Yang XM. suggested by lperfetto human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Lian WX., Yin RH., Kong XZ., Zhang T., Huang XH., Zheng WW., Yang Y., Zhan YQ., Xu WX., Yu M., Ge CH., Guo JT., Li CY., Yang XM. suggested by lperfetto FEBS Lett. (0014-5793) 2012 THAP11, a novel binding protein of PCBP1, negatively regulates CD44 alternative splicing and cell invasion in a human hepatoma cell line. THAP11, a novel binding protein of PCBP1, negatively regulates CD44 alternative splicing and cell invasion in a human hepatoma cell line. FEBS Lett. (0014-5793) 2012 Lian WX., Yin RH., Kong XZ., Zhang T., Huang XH., Zheng WW., Yang Y., Zhan YQ., Xu WX., Yu M., Ge CH., Guo JT., Li CY., Yang XM. suggested by lperfetto human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Lian WX., Yin RH., Kong XZ., Zhang T., Huang XH., Zheng WW., Yang Y., Zhan YQ., Xu WX., Yu M., Ge CH., Guo JT., Li CY., Yang XM. suggested by lperfetto FEBS Lett. (0014-5793) 2012 tha11_human THAP domain-containing protein 11 THAP11 HRIHFB2206 protein protein human Homo sapiens Human MPGFTCCVPGCYNNSHRDKALHFYTFPKDAELRRLWLKNVSRAGVSGCFSTFQPTTGHRLCSVHFQGGRKTYTVRVPTIFPLRGVNERKVARRPAGAAAARRRQQQQQQQQQQQQQQQQQQQQQQQQQQQQQSSPSASTAQTAQLQPNLVSASAAVLLTLQATVDSSQAPGSVQPAPITPTGEDVKPIDLTVQVEFAAAEGAAAAAAASELQAATAGLEAAECPMGPQLVVVGEEGFPDTGSDHSYSLSSGTTEEELLRKLNEQRDILALMEVKMKEMKGSIRHLRLTEAKLREELREKDRLLAMAVIRKKHGM 25235D20CB54D8C2 25235D20CB54D8C2 pcbp1_human Poly(rC)-binding protein 1 PCBP1 Alpha-CP1 Nucleic acid-binding protein SUB2.3 Heterogeneous nuclear ribonucleoprotein E1 protein protein human Homo sapiens Human MDAGVTESGLNVTLTIRLLMHGKEVGSIIGKKGESVKRIREESGARINISEGNCPERIITLTGPTNAIFKAFAMIIDKLEEDINSSMTNSTAASRPPVTLRLVVPATQCGSLIGKGGCKIKEIRESTGAQVQVAGDMLPNSTERAITIAGVPQSVTECVKQICLVMLETLSQSPQGRVMTIPYQPMPASSPVICAGGQDRCSDAAGYPHATHDLEGPPLDAYSIQGQHTISPLDLAKLNQVARQQSHFAMMHGGTGFAGIDSSSPEVKGYWASLDASTQTTHELTIPNNLIGCIIGRQGANINEIRQMSGAQIKIANPVEGSSGRQVTITGSAASISLAQYLINARLSSEKGMGCS DIP protein Q15365 original sequence version: 141 DIP protein Q15365 original sequence version: 116 6D1A261276CA206D thap11-pcbp1 3 THAP11 4 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined PCBP1 5 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f1a homomint mint pcbp1-thap11 3 THAP11 4 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-7907469]C-terminal positions must always be equal to the length of the protein sequence. Actual positions : 131-131, sequence length 314 [EBI-7907469]131-313 PCBP1 5 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain KH domain physical association physical association f2 homomint mint THAP11 can be co-immunoprecipated by PCBP1, N83, C273, while no signal was detected in the immunoprecipitates from cells transfected with C184, suggesting that the first two KH domains (KH1 and KH2) are required for THAP11 binding. To determine the binding domain of THAP11 with PCBP1, HEK293 cells were transfected with the vectors as Fig. 2B indicated. Immunoprecipitations was performed using anti-FLAG antibody. As Fig. 2B shows, the C-terminal 182aa (131-313) of THAP11 was sufficient to interact with PCBP1. thap11-pcbp1-1 1 THAP11 4 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level PCBP1 5 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association f1b homomint mint pcbp1-thap11-1 2 THAP11 4 tag fluorescence tag visualisation by fluorescence tag fluorescence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined PCBP1 5 tag fluorescence tag visualisation by fluorescence tag fluorescence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell rfp tag rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization f1c homomint mint The localizations of THAP11 (green) and PCBP1 (red) were observed through confocal imaging. As shown PCBP1 mainly localizes in cytoplasm with a smaller nuclear diffuse fraction in normal conditions, while THAP11 is a nuclear diffuse protein.