MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Dimer interface rearrangement of the 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase rat liver isoenzyme by cAMP-dependent Ser-32 phosphorylation. Dimer interface rearrangement of the 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase rat liver isoenzyme by cAMP-dependent Ser-32 phosphorylation. FEBS Lett. (0014-5793) 2012 Langer S., Okar DA., Schultz J., Lenzen S., Baltrusch S. suggested by lperfetto chlae-cos Chlorocebus aethiops cos cos fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Langer S., Okar DA., Schultz J., Lenzen S., Baltrusch S. suggested by lperfetto FEBS Lett. (0014-5793) 2012 Dimer interface rearrangement of the 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase rat liver isoenzyme by cAMP-dependent Ser-32 phosphorylation. Dimer interface rearrangement of the 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase rat liver isoenzyme by cAMP-dependent Ser-32 phosphorylation. FEBS Lett. (0014-5793) 2012 Langer S., Okar DA., Schultz J., Lenzen S., Baltrusch S. suggested by lperfetto in vitro In vitro fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Langer S., Okar DA., Schultz J., Lenzen S., Baltrusch S. suggested by lperfetto FEBS Lett. (0014-5793) 2012 Dimer interface rearrangement of the 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase rat liver isoenzyme by cAMP-dependent Ser-32 phosphorylation. Dimer interface rearrangement of the 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase rat liver isoenzyme by cAMP-dependent Ser-32 phosphorylation. FEBS Lett. (0014-5793) 2012 Langer S., Okar DA., Schultz J., Lenzen S., Baltrusch S. suggested by lperfetto chlae-cos Chlorocebus aethiops cos cos 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Langer S., Okar DA., Schultz J., Lenzen S., Baltrusch S. suggested by lperfetto FEBS Lett. (0014-5793) 2012 f261_rat 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 6PF-2-K/Fru-2,6-P2ase liver isozyme Pfkfb1 protein protein rat Rattus norvegicus (Rat) Rat MSREMGELTQTRLQKIWIPHSSSSSVLQRRRGSSIPQFTNSPTMVIMVGLPARGKTYISTKLTRYLNWIGTPTKVFNLGQYRREAVSYRNYEFFRPDNTEAQLIRKQCALAALKDVHKYLSREEGHVAVFDATNTTRERRSLILQFAKEHGYKVFFIESICNDPEIIAENIKQVKLGSPDYIDCDQEKVLEDFLKRIECYEINYQPLDEELDSHLSYIKIFDVGTRYMVNRVQDHVQSRTAYYLMNIHVTPRSIYLCRHGESELNLRGRIGGDSGLSARGKQYAYALANFIRSQGISSLKVWTSHMKRTIQTAEALGVPYEQWKALNEIDAGVCEEMTYEEIQEHYPEEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKSSDELPYLKCPLHTVLKLTPVAYGCRVESIYLNVEAVNTHRDKPENVDITREAEEALDTVPAHY E307AF13945E0FCD pfkfb1-pfkfb1 3 PFK-2/FBPase-2 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P07953:p.Ser33Ala mutation increasing interaction mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A Tue Jul 12 10:11:08 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation increasing' Wed May 02 20:08:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser33ala' Tue Jun 30 18:28:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser33Ala' P07953:p.His259Ala mutation increasing interaction mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain H A Tue Jul 12 10:11:09 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation increasing' Wed May 02 15:29:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his259ala' Tue Jun 30 17:00:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His259Ala' PFK-2/FBPase-2 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P07953:p.Ser33Ala mutation increasing interaction mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A Tue Jul 12 10:56:36 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation increasing' Wed May 02 16:49:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser33ala' Tue Jun 30 18:08:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser33Ala' P07953:p.His259Ala mutation increasing interaction mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain H A Tue Jul 12 10:11:11 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation increasing' Wed May 02 15:28:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his259ala' Tue Jun 30 16:49:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His259Ala' physical association physical association f2 homomint mint pfkfb1-pfkfb1-1 1 PFK-2/FBPase-2 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed living cell living cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined PFK-2/FBPase-2 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined proximity proximity close-contact colocalization neighbourhood interaction homomint mint f4a f4b f4c Confirming our observation using the mammalian two-hybrid analysis, forskolin treatment for 2 h resulted only in case of the S32A/H258A mutant in a further significant increase in FRETN of almost 100 %. pfkfb1-pfkfb1-2 2 PFK-2/FBPase-2 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role fluorescence donor fluorescence donor partially purified partially purified fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined PFK-2/FBPase-2 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept partially purified partially purified fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f5 homomint mint To avoid the quenching by endogenous PFK-2/FBPase-2, Dendra2 fusion proteins of the liver wild-type enzyme and the S32A/H258A mutant were isolated from COS cells before FRET analysis.A significant FRET signal was detected between wild-type PFK-2/FBPase-2 proteins immediately used after isolation (253.4 ± 29.0 cps), but only a minor signal between S32A/H258A mutant proteins (113.4 ± 34.4 cps) (Fig. 5).