mpidb The Microbial Protein Interaction Database Enterococcus faecalis PrgJ, a VirB4-like ATPase, mediates pCF10 conjugative transfer through substrate binding. Enterococcus faecalis PrgJ, a VirB4-like ATPase, mediates pCF10 conjugative transfer through substrate binding. J. Bacteriol. (0021-9193) 2012 imex curation Li F., Alvarez-Martinez C., Chen Y., Choi K.J., Yeo H.J., Christie P.J. 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions ecoli Escherichia coli (strain K12) molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Li F., Alvarez-Martinez C., Chen Y., Choi K.J., Yeo H.J., Christie P.J. Accepted 2012-DEC-05 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. J. Bacteriol. (0021-9193) 2012 Only protein-protein interactions imex curation Enterococcus faecalis PrgJ, a VirB4-like ATPase, mediates pCF10 conjugative transfer through substrate binding. Enterococcus faecalis PrgJ, a VirB4-like ATPase, mediates pCF10 conjugative transfer through substrate binding. J. Bacteriol. (0021-9193) 2012 imex curation Li F., Alvarez-Martinez C., Chen Y., Choi K.J., Yeo H.J., Christie P.J. 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Li F., Alvarez-Martinez C., Chen Y., Choi K.J., Yeo H.J., Christie P.J. Accepted 2012-DEC-05 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. J. Bacteriol. (0021-9193) 2012 Only protein-protein interactions imex curation q5g3n9_entfl pcfC protein protein entfl Enterococcus faecalis Streptococcus faecalis MQTYKKELKPYLYLGVFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFSVGVLGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKLQKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTFNVFEYMHSELDVDRVLEAVTEATKEVEKTTGDDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADMLRHLKRKDKNVPSPVEEWFEELNEAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIRTDTMDIDSWNEEKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRVLEEGKELLHVRFLIDEFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYRNGWQNILNGCACLLYLGGDEEETTKYLSARAGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRADELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSELKEETHV 64BB35098E684741 q7x5p3_entfl pcfG protein protein entfl Enterococcus faecalis Streptococcus faecalis MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPYEEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKIHEIGYNTVMELTGGKYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKRVFEQISDKIAAKEGAKIIEKSPKQTHTKYTKWQTESIYKQKIKSRLDFLLEQSSSINDFLEKAEALNLSADFSNKWTTYRLLDEPQIKNTRSRSLSKSDPTRYNYEKIIERLKENKNVLTVEECVKRYEEKNEQEKNNFDYQFTIEPWQLSHKTERGYYINIDYGYGNSGKLFVGGYKVDPLDNGNYNIYIKRNEYFYFMSDKDATKSKYLTGASLIKQLRLYNGQTPLKREPVMQTIDELVSAINFLAANEIEDTRQLKLLEEKLEVAFLEAEKTLETLDEKMLELHQLSNLLLENELQGDPELIQKKLKTLLPDATLAEFSYEDVRGEIEAIKTSQTLLESKLERTRNEINQLHEIQAVQEKEPEKQTDIKPKL 1FF37CCFEC4E608A q5g3p5_entfl prgJ EU507_13880 protein protein entfl Enterococcus faecalis Streptococcus faecalis MHIVEDEFSKCYKLGEVDYEIAVEEDQLNTVMGYAEGLNTLDKNSRYQLLVVNKREEESLMEDVLLAYQGDHLDNYRQEINEIITKQYEQDERNFKIEKYAIFTTKSSSMKQANKELEGIMQNFANRFEANQIKLEVTPMTGLERLSLFSYFLRHGQYFGTSYQDIAVSGLTSKAFIVPSKIKFPNNKAYFRLGENYAAVLTIQQYPKYLEDKLIKELCATGRELAISIHARPYDMMEARKAIQGTKTLNDVAIQKQQKQNFRSGVSEDAISGEAKEIQETTNALMDEIKNNGQKLFNGLFSVFLIAETEKELLEAVESVKNVGATWQVVFDTVDNYKEEALNTILPIGKPYLDVEMSYMRDLTTTNIATQVPFTNVELQSPTGQFYGRNQLTKNMITIDRKKDLITPSGLIFGTSGSGKGMATKWEILSTRLKFPKDRFLIVDPESEYLPIGKELDAEILDISTGTKNHLNILGMVDKQLLDEEDQELDLVKEKSNLLSTMFESLLKSYTDVDAGLVDRVTLLTYERFENKETEPTLVDWFRILKEQTDEGAKELANKVETYCVGSQDIFAHQTNIDLTAPFIVFNTKKLDDRLKKFAMKVILDQLWKQVVSGQGKVTSRLYFDELQVNFTTEEEAEWFLNLWARIRKYGAVTTGITQNPTTLLDSKAGQKMIANSEFLILLRQKPIDSQRLVEILSLTPALKKYINDRAPQGTGLISAGGVVVPFENPIPKETHLFEIMNTDA 71FFE061BE55701A q5g3n6_entfl pcfF DAI13_16755 protein protein entfl Enterococcus faecalis Streptococcus faecalis MENKQKLKRPIQRIVRLSEEENNLIKRKIEESFFPNFQNFALHLLIQGEIRHVDYSELNRLTTEIHKIGININQMARLANQFHEISSEDIKDLTDKVQSLNALVQSELNKLIKRKDQS 24629587CD69EB17 prgj-pcff 2 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 3 prgj-prgj-1 1 5 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association figure 2A pcff-prgj 2 5 unspecified role unspecified role prey prey region Q5G3P5-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction Fig. 3 pcfg-prgj 2 5 unspecified role unspecified role prey prey q5g3p5-- Q5G3P5-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig 3 pcfc-prgj 2 5 unspecified role unspecified role prey prey q5g3p5-- Q5G3P5-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 3 unspecified role unspecified role bait bait q5g3n9-- Q5G3N9-- gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 3 prgj-pcfg-1 2 4 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role bait bait q5g3p5-- Q5G3P5-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Fig. 3 prgj-pcfc-1 2 5 unspecified role unspecified role bait bait q5g3p5-- Q5G3P5-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association table 3