IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Structural evidence that colicin A protein binds to a novel binding site of TolA protein in Escherichia coli periplasm. Structural evidence that colicin A protein binds to a novel binding site of TolA protein in Escherichia coli periplasm. J. Biol. Chem. (0021-9258) 2012 imex curation Li C., Zhang Y., Vankemmelbeke M., Hecht O., Aleanizy F.S., Macdonald C., Moore G.R., James R., Penfold C.N. chris.penfold@nottingham.ac.uk 16-Apr-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Li C., Zhang Y., Vankemmelbeke M., Hecht O., Aleanizy F.S., Macdonald C., Moore G.R., James R., Penfold C.N. chris.penfold@nottingham.ac.uk Accepted 2013-MAR-26 by PPORRAS 16-Apr-2013: Contacted by IntAct-Help. J. Biol. Chem. (0021-9258) 2012 Only protein-protein interactions imex curation Structural evidence that colicin A protein binds to a novel binding site of TolA protein in Escherichia coli periplasm. Structural evidence that colicin A protein binds to a novel binding site of TolA protein in Escherichia coli periplasm. J. Biol. Chem. (0021-9258) 2012 imex curation Li C., Zhang Y., Vankemmelbeke M., Hecht O., Aleanizy F.S., Macdonald C., Moore G.R., James R., Penfold C.N. chris.penfold@nottingham.ac.uk 16-Apr-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Li C., Zhang Y., Vankemmelbeke M., Hecht O., Aleanizy F.S., Macdonald C., Moore G.R., James R., Penfold C.N. chris.penfold@nottingham.ac.uk Accepted 2013-MAR-26 by PPORRAS 16-Apr-2013: Contacted by IntAct-Help. J. Biol. Chem. (0021-9258) 2012 Only protein-protein interactions imex curation Structural evidence that colicin A protein binds to a novel binding site of TolA protein in Escherichia coli periplasm. Structural evidence that colicin A protein binds to a novel binding site of TolA protein in Escherichia coli periplasm. J. Biol. Chem. (0021-9258) 2012 imex curation Li C., Zhang Y., Vankemmelbeke M., Hecht O., Aleanizy F.S., Macdonald C., Moore G.R., James R., Penfold C.N. chris.penfold@nottingham.ac.uk 16-Apr-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Li C., Zhang Y., Vankemmelbeke M., Hecht O., Aleanizy F.S., Macdonald C., Moore G.R., James R., Penfold C.N. chris.penfold@nottingham.ac.uk Accepted 2013-MAR-26 by PPORRAS 16-Apr-2013: Contacted by IntAct-Help. J. Biol. Chem. (0021-9258) 2012 Only protein-protein interactions imex curation tola_ecoli Tol-Pal system protein TolA tolA cim excC lky b0739 JW0729 protein protein ecoli Escherichia coli (strain K12) MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGGGGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQERLKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAAKKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAAAAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQAVYEVFKNAPLDFKP DIP protein P19934 original sequence version: 89 8B2F52B4B97C655E cea_citfr Colicin-A caa protein protein citfr Citrobacter freundii MPGFNYGGKGDGTGWSSERGSGPEPGGGSHGNSGGHDRGDSSNVGNESVTVMKPGDSYNTPWGKVIINAAGQPTMNGTVMTADNSSMVPYGRGFTRVLNSLVNNPVSPAGQNGGKSPVQTAVENYLMVQSGNLPPGYWLSNGKVMTEVREERTSGGGGKNGNERTWTVKVPREVPQLTASYNEGMRIRQEAADRARAEANARALAEEEARAIASGKSKAEFDAGKRVEAAQAAINTAQLNVNNLSGAVSAANQVITQKQAEMTPLKNELAAANQRVQETLKFINDPIRSRIHFNMRSGLIRAQHNVDTKQNEINAAVANRDALNSQLSQANNILQNARNEKSAADAALSAATAQRLQAEAALRAAAEAAEKARQRQAEEAERQRQAMEVAEKAKDERELLEKTSELIAGMGDKIGEHLGDKYKAIAKDIADNIKNFQGKTIRSFDDAMASLNKITANPAMKINKADRDALVNAWKHVDAQDMANKLGNLSKAFKVADVVMKVEKVREKSIEGYETGNWGPLMLEVESWVLSGIASSVALGIFSATLGAYALSLGVPAIAVGIAGILLAAVVGALIDDKFADALNNEIIRPAH B80FA1F52A8CFC5D caa-tola-3 3 5 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role neutral component neutral component lys-351 melys methylated lysine MeLys certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 1, Table 1 2.65 A R factor: 24.4 R free: 27.3% caa-tola-4 1 4 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role neutral component neutral component P04480:p.Tyr58Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain Y A Wed May 02 19:10:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr58ala' Tue Jun 30 19:10:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr58Ala' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure S5 caa-tola-5 2 5 unspecified role unspecified role neutral component neutral component region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure S6