IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ NKX2-1/TITF1/TTF-1-Induced ROR1 Is Required to Sustain EGFR Survival Signaling in Lung Adenocarcinoma. NKX2-1/TITF1/TTF-1-Induced ROR1 Is Required to Sustain EGFR Survival Signaling in Lung Adenocarcinoma. Cancer Cell (1535-6108) 2012 imex curation Yamaguchi T., Yanagisawa K., Sugiyama R., Hosono Y., Shimada Y., Arima C., Kato S., Tomida S., Suzuki M., Osada H., Takahashi T. Cancer - Interactions investigated in the context of cancer ODDity:- No Expt number =6 was ever created for some reason - it was NOT ever deleted! (also there were two identical Experiment-3s - one deleted) Fig 4G not included - Kinase Assay - as from suppliers info the kinase substrate can be phosp-ed by several kinases. tak@med.nagoya-u.ac.jp 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Yamaguchi T., Yanagisawa K., Sugiyama R., Hosono Y., Shimada Y., Arima C., Kato S., Tomida S., Suzuki M., Osada H., Takahashi T. Cancer - Interactions investigated in the context of cancer ODDity:- No Expt number =6 was ever created for some reason - it was NOT ever deleted! (also there were two identical Experiment-3s - one deleted) tak@med.nagoya-u.ac.jp Accepted 2012-APR-20 by ORCHARD 20-Jun-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation NKX2-1/TITF1/TTF-1-Induced ROR1 Is Required to Sustain EGFR Survival Signaling in Lung Adenocarcinoma. NKX2-1/TITF1/TTF-1-Induced ROR1 Is Required to Sustain EGFR Survival Signaling in Lung Adenocarcinoma. Cancer Cell (1535-6108) 2012 imex curation Yamaguchi T., Yanagisawa K., Sugiyama R., Hosono Y., Shimada Y., Arima C., Kato S., Tomida S., Suzuki M., Osada H., Takahashi T. Cancer - Interactions investigated in the context of cancer ODDity:- No Expt number =6 was ever created for some reason - it was NOT ever deleted! (also there were two identical Experiment-3s - one deleted) Fig 4G not included - Kinase Assay - as from suppliers info the kinase substrate can be phosp-ed by several kinases. tak@med.nagoya-u.ac.jp 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions human-sk-lc-5 SK-LC-5 Human lung adenocarcinoma cell line sk-lc-5 SK-LC-5 Human lung adenocarcinoma cell line ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Yamaguchi T., Yanagisawa K., Sugiyama R., Hosono Y., Shimada Y., Arima C., Kato S., Tomida S., Suzuki M., Osada H., Takahashi T. Cancer - Interactions investigated in the context of cancer ODDity:- No Expt number =6 was ever created for some reason - it was NOT ever deleted! (also there were two identical Experiment-3s - one deleted) tak@med.nagoya-u.ac.jp Accepted 2012-APR-20 by ORCHARD 20-Jun-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation NKX2-1/TITF1/TTF-1-Induced ROR1 Is Required to Sustain EGFR Survival Signaling in Lung Adenocarcinoma. NKX2-1/TITF1/TTF-1-Induced ROR1 Is Required to Sustain EGFR Survival Signaling in Lung Adenocarcinoma. Cancer Cell (1535-6108) 2012 imex curation Yamaguchi T., Yanagisawa K., Sugiyama R., Hosono Y., Shimada Y., Arima C., Kato S., Tomida S., Suzuki M., Osada H., Takahashi T. Cancer - Interactions investigated in the context of cancer ODDity:- No Expt number =6 was ever created for some reason - it was NOT ever deleted! (also there were two identical Experiment-3s - one deleted) Fig 4G not included - Kinase Assay - as from suppliers info the kinase substrate can be phosp-ed by several kinases. tak@med.nagoya-u.ac.jp 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yamaguchi T., Yanagisawa K., Sugiyama R., Hosono Y., Shimada Y., Arima C., Kato S., Tomida S., Suzuki M., Osada H., Takahashi T. Cancer - Interactions investigated in the context of cancer ODDity:- No Expt number =6 was ever created for some reason - it was NOT ever deleted! (also there were two identical Experiment-3s - one deleted) tak@med.nagoya-u.ac.jp Accepted 2012-APR-20 by ORCHARD 20-Jun-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation NKX2-1/TITF1/TTF-1-Induced ROR1 Is Required to Sustain EGFR Survival Signaling in Lung Adenocarcinoma. NKX2-1/TITF1/TTF-1-Induced ROR1 Is Required to Sustain EGFR Survival Signaling in Lung Adenocarcinoma. Cancer Cell (1535-6108) 2012 imex curation Yamaguchi T., Yanagisawa K., Sugiyama R., Hosono Y., Shimada Y., Arima C., Kato S., Tomida S., Suzuki M., Osada H., Takahashi T. Cancer - Interactions investigated in the context of cancer ODDity:- No Expt number =6 was ever created for some reason - it was NOT ever deleted! (also there were two identical Experiment-3s - one deleted) Fig 4G not included - Kinase Assay - as from suppliers info the kinase substrate can be phosp-ed by several kinases. tak@med.nagoya-u.ac.jp 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Yamaguchi T., Yanagisawa K., Sugiyama R., Hosono Y., Shimada Y., Arima C., Kato S., Tomida S., Suzuki M., Osada H., Takahashi T. Cancer - Interactions investigated in the context of cancer ODDity:- No Expt number =6 was ever created for some reason - it was NOT ever deleted! (also there were two identical Experiment-3s - one deleted) tak@med.nagoya-u.ac.jp Accepted 2012-APR-20 by ORCHARD 20-Jun-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation NKX2-1/TITF1/TTF-1-Induced ROR1 Is Required to Sustain EGFR Survival Signaling in Lung Adenocarcinoma. NKX2-1/TITF1/TTF-1-Induced ROR1 Is Required to Sustain EGFR Survival Signaling in Lung Adenocarcinoma. Cancer Cell (1535-6108) 2012 imex curation Yamaguchi T., Yanagisawa K., Sugiyama R., Hosono Y., Shimada Y., Arima C., Kato S., Tomida S., Suzuki M., Osada H., Takahashi T. Cancer - Interactions investigated in the context of cancer ODDity:- No Expt number =6 was ever created for some reason - it was NOT ever deleted! (also there were two identical Experiment-3s - one deleted) Fig 4G not included - Kinase Assay - as from suppliers info the kinase substrate can be phosp-ed by several kinases. tak@med.nagoya-u.ac.jp 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Yamaguchi T., Yanagisawa K., Sugiyama R., Hosono Y., Shimada Y., Arima C., Kato S., Tomida S., Suzuki M., Osada H., Takahashi T. Cancer - Interactions investigated in the context of cancer ODDity:- No Expt number =6 was ever created for some reason - it was NOT ever deleted! (also there were two identical Experiment-3s - one deleted) tak@med.nagoya-u.ac.jp Accepted 2012-APR-20 by ORCHARD 20-Jun-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation NKX2-1/TITF1/TTF-1-Induced ROR1 Is Required to Sustain EGFR Survival Signaling in Lung Adenocarcinoma. NKX2-1/TITF1/TTF-1-Induced ROR1 Is Required to Sustain EGFR Survival Signaling in Lung Adenocarcinoma. Cancer Cell (1535-6108) 2012 imex curation Yamaguchi T., Yanagisawa K., Sugiyama R., Hosono Y., Shimada Y., Arima C., Kato S., Tomida S., Suzuki M., Osada H., Takahashi T. Cancer - Interactions investigated in the context of cancer ODDity:- No Expt number =6 was ever created for some reason - it was NOT ever deleted! (also there were two identical Experiment-3s - one deleted) Fig 4G not included - Kinase Assay - as from suppliers info the kinase substrate can be phosp-ed by several kinases. tak@med.nagoya-u.ac.jp 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay western blot western blot Immuno blot Immuno blot Yamaguchi T., Yanagisawa K., Sugiyama R., Hosono Y., Shimada Y., Arima C., Kato S., Tomida S., Suzuki M., Osada H., Takahashi T. Cancer - Interactions investigated in the context of cancer ODDity:- No Expt number =6 was ever created for some reason - it was NOT ever deleted! (also there were two identical Experiment-3s - one deleted) c-Src was immunoprecipitated from ROR1-negative NCI-H23 or 293T cells. KD-c-Src was immunoprecipitated from NIH-3T3 cells, and all c-Srcs were used as substrates for in vitro ROR1 kinase assays. For c-SRC (mouse) kinase dead mutant the residue numbering was derived from Broome et al.,-1996 (stated to be K297M); numbering entered here is given in relation to current UniProt entry, allowing for the fact "the neuronal insert of 6 amino acids was removed by deletion" tak@med.nagoya-u.ac.jp Accepted 2012-APR-20 by ORCHARD 20-Jun-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation NKX2-1/TITF1/TTF-1-Induced ROR1 Is Required to Sustain EGFR Survival Signaling in Lung Adenocarcinoma. NKX2-1/TITF1/TTF-1-Induced ROR1 Is Required to Sustain EGFR Survival Signaling in Lung Adenocarcinoma. Cancer Cell (1535-6108) 2012 imex curation Yamaguchi T., Yanagisawa K., Sugiyama R., Hosono Y., Shimada Y., Arima C., Kato S., Tomida S., Suzuki M., Osada H., Takahashi T. Cancer - Interactions investigated in the context of cancer ODDity:- No Expt number =6 was ever created for some reason - it was NOT ever deleted! (also there were two identical Experiment-3s - one deleted) Fig 4G not included - Kinase Assay - as from suppliers info the kinase substrate can be phosp-ed by several kinases. tak@med.nagoya-u.ac.jp 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions human-nci_h1975 Homo sapiens NCI-H1975 Adenocarcinoma nci_h1975 NCI-H1975 NSCLC - Adenocarcinoma cell line - carries double EGFR mutations L858R and T790M, the latter of which is a well-recognized mutation that confers resistance to EGFR TKI. http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=CRL-5908&Template=cellBiology anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yamaguchi T., Yanagisawa K., Sugiyama R., Hosono Y., Shimada Y., Arima C., Kato S., Tomida S., Suzuki M., Osada H., Takahashi T. Cancer - Interactions investigated in the context of cancer ODDity:- No Expt number =6 was ever created for some reason - it was NOT ever deleted! (also there were two identical Experiment-3s - one deleted) Figure 4F:- The authors of this paper believe that the molecule "p85 (PI3K)" is p85A - (UniProt ID = P27986 Gene:PIK3R1) as detected by the polyclonal antibody from Millipore cat. No = 06-497. tak@med.nagoya-u.ac.jp Accepted 2012-APR-20 by ORCHARD Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation p05480-2 Neuronal proto-oncogene tyrosine-protein kinase Src Proto-oncogene c-Src pp60c-src Src protein protein mouse Mus musculus Mouse MGSNKSKPKDASQRRRSLEPSENVHGAGGAFPASQTPSKPASADGHRGPSAAFVPPAAEPKLFGGFNSSDTVTSPQRAGPLAGGVTTFVALYDYESRTETDLSFKKGERLQIVNNTEGDWWLAHSLSTGQTGYIPSNYVAPSDSIQAEEWYFGKITRRESERLLLNAENPRGTFLVRESETTKGAYCLSVSDFDNAKGLNVKHYKIRKLDSGGFYITSRTQFNSLQQLVAYYSKHADGLCHRLTTVCPTSKPQTQGLAKDAWEIPRESLRLEVKLGQGCFGEVWMGTWNGTTRVAIKTLKPGTMSPEAFLQEAQVMKKLRHEKLVQLYAVVSEEPIYIVTEYMNKGSLLDFLKGETGKYLRLPQLVDMSAQIASGMAYVERMNYVHRDLRAANILVGENLVCKVADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELTTKGRVPYPGMVNREVLDQVERGYRMPCPPECPESLHDLMCQCWRKEPEERPTFEYLQAFLEDYFTSTEPQYQPGENL 22CB5C6BE7A061C0 src_mouse Neuronal proto-oncogene tyrosine-protein kinase Src Src pp60c-src Proto-oncogene c-Src protein protein mouse Mus musculus Mouse MGSNKSKPKDASQRRRSLEPSENVHGAGGAFPASQTPSKPASADGHRGPSAAFVPPAAEPKLFGGFNSSDTVTSPQRAGPLAGGVTTFVALYDYESRTETDLSFKKGERLQIVNNTEGDWWLAHSLSTGQTGYIPSNYVAPSDSIQAEEWYFGKITRRESERLLLNAENPRGTFLVRESETTKGAYCLSVSDFDNAKGLNVKHYKIRKLDSGGFYITSRTQFNSLQQLVAYYSKHADGLCHRLTTVCPTSKPQTQGLAKDAWEIPRESLRLEVKLGQGCFGEVWMGTWNGTTRVAIKTLKPGTMSPEAFLQEAQVMKKLRHEKLVQLYAVVSEEPIYIVTEYMNKGSLLDFLKGETGKYLRLPQLVDMSAQIASGMAYVERMNYVHRDLRAANILVGENLVCKVADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELTTKGRVPYPGMVNREVLDQVERGYRMPCPPECPESLHDLMCQCWRKEPEERPTFEYLQAFLEDYFTSTEPQYQPGENL DIP protein P05480 original sequence version: 163 DIP protein P05480 original sequence version: 99 DIP protein P05480 original sequence version: 128 DIP protein P05480 original sequence version: 136 DIP protein P05480 original sequence version: 176 22CB5C6BE7A061C0 egfr_human Epidermal growth factor receptor ERBB1 EGFR Receptor tyrosine-protein kinase erbB-1 Proto-oncogene c-ErbB-1 ERBB HER1 protein protein human Homo sapiens Human MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGRCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTPSGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA DIP protein P00533 original sequence version: 213 DIP protein P00533 original sequence version: 214 DIP protein P00533 original sequence version: 119 DIP protein P00533 original sequence version: 207 DIP protein P00533 original sequence version: 132 DIP protein P00533-1 original sequence version: 197 DIP protein P00533 original sequence version: 163 DIP protein P00533-1 original sequence version: 159 DIP protein P00533 original sequence version: 184 DIP protein P00533-1 original sequence version: 174 DIP protein P00533-1 original sequence version: 191 DIP protein P00533 original sequence version: 218 DIP protein P00533 original sequence version: 221 DIP protein P00533-1 original sequence version: 221 DIP protein P00533 original sequence version: 226 DIP protein P00533 original sequence version: 166 DIP protein P00533-1 original sequence version: 166 DIP protein P00533 original sequence version: 194 DIP protein P00533-1 original sequence version: 194 DIP protein P00533-1 original sequence version: 224 DIP protein P00533 original sequence version: 189 DIP protein P00533-1 original sequence version: 207 DIP protein P00533 original sequence version: 177 DIP protein P00533 original sequence version: 168 D8A2A50B4EFB6ED2 nkx21_human Homeobox protein Nkx-2.1 NKX2-1 TITF1 NKX2A TTF1 Homeobox protein NK-2 homolog A Thyroid transcription factor 1 Thyroid nuclear factor 1 Thyroid-specific enhancer-binding protein protein protein human Homo sapiens Human MSMSPKHTTPFSVSDILSPLEESYKKVGMEGGGLGAPLAAYRQGQAAPPTAAMQQHAVGHHGAVTAAYHMTAAGVPQLSHSAVGGYCNGNLGNMSELPPYQDTMRNSASGPGWYGANPDPRFPAISRFMGPASGMNMSGMGGLGSLGDVSKNMAPLPSAPRRKRRVLFSQAQVYELERRFKQQKYLSAPEREHLASMIHLTPTQVKIWFQNHRYKMKRQAKDKAAQQQLQQDSGGGGGGGGTGCPQQQQAQQQSPRRVAVPVLVKDGKPCQAGAPAPGAASLQGHAQQQAQHQAQAAQAAAAAISVGSGGAGLGAHPGHQPGSAGQSPDLAHHAASPAALQGQVSSLSHLNSSGSDYGTMSCSTLLYGRTW 5F1E3B40A1BBD862 src_human Proto-oncogene tyrosine-protein kinase Src pp60c-src SRC SRC1 Proto-oncogene c-Src protein protein human Homo sapiens Human MGSNKSKPKDASQRRRSLEPAENVHGAGGGAFPASQTPSKPASADGHRGPSAAFAPAAAEPKLFGGFNSSDTVTSPQRAGPLAGGVTTFVALYDYESRTETDLSFKKGERLQIVNNTEGDWWLAHSLSTGQTGYIPSNYVAPSDSIQAEEWYFGKITRRESERLLLNAENPRGTFLVRESETTKGAYCLSVSDFDNAKGLNVKHYKIRKLDSGGFYITSRTQFNSLQQLVAYYSKHADGLCHRLTTVCPTSKPQTQGLAKDAWEIPRESLRLEVKLGQGCFGEVWMGTWNGTTRVAIKTLKPGTMSPEAFLQEAQVMKKLRHEKLVQLYAVVSEEPIYIVTEYMSKGSLLDFLKGETGKYLRLPQLVDMAAQIASGMAYVERMNYVHRDLRAANILVGENLVCKVADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELTTKGRVPYPGMVNREVLDQVERGYRMPCPPECPESLHDLMCQCWRKEPEERPTFEYLQAFLEDYFTSTEPQYQPGENL DIP protein P12931 original sequence version: 188 DIP protein P12931 original sequence version: 189 DIP protein P12931 original sequence version: 190 DIP protein P12931 original sequence version: 191 DIP protein P12931 original sequence version: 115 DIP protein P12931 original sequence version: 116 DIP protein P12931 original sequence version: 164 DIP protein P12931 original sequence version: 142 DIP protein P12931 original sequence version: 143 DIP protein P12931 original sequence version: 150 DIP protein P12931 original sequence version: 160 DIP protein P12931 original sequence version: 184 DIP protein P12931 original sequence version: 155 DIP protein P12931-1 original sequence version: 155 DIP protein P12931 original sequence version: 202 C1908084683E5DE8 ror1_human Inactive tyrosine-protein kinase transmembrane receptor ROR1 ROR1 NTRKR1 Neurotrophic tyrosine kinase, receptor-related 1 protein protein human Homo sapiens Human MHRPRRRGTRPPLLALLAALLLAARGAAAQETELSVSAELVPTSSWNISSELNKDSYLTLDEPMNNITTSLGQTAELHCKVSGNPPPTIRWFKNDAPVVQEPRRLSFRSTIYGSRLRIRNLDTTDTGYFQCVATNGKEVVSSTGVLFVKFGPPPTASPGYSDEYEEDGFCQPYRGIACARFIGNRTVYMESLHMQGEIENQITAAFTMIGTSSHLSDKCSQFAIPSLCHYAFPYCDETSSVPKPRDLCRDECEILENVLCQTEYIFARSNPMILMRLKLPNCEDLPQPESPEAANCIRIGIPMADPINKNHKCYNSTGVDYRGTVSVTKSGRQCQPWNSQYPHTHTFTALRFPELNGGHSYCRNPGNQKEAPWCFTLDENFKSDLCDIPACDSKDSKEKNKMEILYILVPSVAIPLAIALLFFFICVCRNNQKSSSAPVQRQPKHVRGQNVEMSMLNAYKPKSKAKELPLSAVRFMEELGECAFGKIYKGHLYLPGMDHAQLVAIKTLKDYNNPQQWTEFQQEASLMAELHHPNIVCLLGAVTQEQPVCMLFEYINQGDLHEFLIMRSPHSDVGCSSDEDGTVKSSLDHGDFLHIAIQIAAGMEYLSSHFFVHKDLAARNILIGEQLHVKISDLGLSREIYSADYYRVQSKSLLPIRWMPPEAIMYGKFSSDSDIWSFGVVLWEIFSFGLQPYYGFSNQEVIEMVRKRQLLPCSEDCPPRMYSLMTECWNEIPSRRPRFKDIHVRLRSWEGLSSHTSSTTPSGGNATTQTTSLSASPVSNLSNPRYPNYMFPSQGITPQGQIAGFIGPPIPQNQRFIPINGYPIPPGYAAFPAAHYQPTGPPRVIQHCPPPKSRSPSSASGSTSTGHVTSLPSSGSNQEANIPLLPHMSIPNHPGGMGITVFGNKSQKPYKIDSKQASLLGDANIHGHTESMISAEL 0EC0E4762A90C6C7 p85a_human Phosphatidylinositol 3-kinase regulatory subunit alpha PIK3R1 GRB1 Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha protein protein human Homo sapiens Human MSAEGYQYRALYDYKKEREEDIDLHLGDILTVNKGSLVALGFSDGQEARPEEIGWLNGYNETTGERGDFPGTYVEYIGRKKISPPTPKPRPPRPLPVAPGSSKTEADVEQQALTLPDLAEQFAPPDIAPPLLIKLVEAIEKKGLECSTLYRTQSSSNLAELRQLLDCDTPSVDLEMIDVHVLADAFKRYLLDLPNPVIPAAVYSEMISLAPEVQSSEEYIQLLKKLIRSPSIPHQYWLTLQYLLKHFFKLSQTSSKNLLNARVLSEIFSPMLFRFSAASSDNTENLIKVIEILISTEWNERQPAPALPPKPPKPTTVANNGMNNNMSLQDAEWYWGDISREEVNEKLRDTADGTFLVRDASTKMHGDYTLTLRKGGNNKLIKIFHRDGKYGFSDPLTFSSVVELINHYRNESLAQYNPKLDVKLLYPVSKYQQDQVVKEDNIEAVGKKLHEYNTQFQEKSREYDRLYEEYTRTSQEIQMKRTAIEAFNETIKIFEEQCQTQERYSKEYIEKFKREGNEKEIQRIMHNYDKLKSRISEIIDSRRRLEEDLKKQAAEYREIDKRMNSIKPDLIQLRKTRDQYLMWLTQKGVRQKKLNEWLGNENTEDQYSLVEDDEDLPHHDEKTWNVGSSNRNKAENLLRGKRDGTFLVRESSKQGCYACSVVVDGEVKHCVINKTATGYGFAEPYNLYSSLKELVLHYQHTSLVQHNDSLNVTLAYPVYAQQRR DIP protein P27986 original sequence version: 181 DIP protein P27986 original sequence version: 184 DIP protein P27986-1 original sequence version: 135 DIP protein P27986 original sequence version: 135 DIP protein P27986-1 original sequence version: 145 DIP protein P27986-1 original sequence version: 147 DIP protein P27986 original sequence version: 191 B9DAD8416C33140F erbb3_human Receptor tyrosine-protein kinase erbB-3 Tyrosine kinase-type cell surface receptor HER3 ERBB3 HER3 Proto-oncogene-like protein c-ErbB-3 protein protein human Homo sapiens Human MRANDALQVLGLLFSLARGSEVGNSQAVCPGTLNGLSVTGDAENQYQTLYKLYERCEVVMGNLEIVLTGHNADLSFLQWIREVTGYVLVAMNEFSTLPLPNLRVVRGTQVYDGKFAIFVMLNYNTNSSHALRQLRLTQLTEILSGGVYIEKNDKLCHMDTIDWRDIVRDRDAEIVVKDNGRSCPPCHEVCKGRCWGPGSEDCQTLTKTICAPQCNGHCFGPNPNQCCHDECAGGCSGPQDTDCFACRHFNDSGACVPRCPQPLVYNKLTFQLEPNPHTKYQYGGVCVASCPHNFVVDQTSCVRACPPDKMEVDKNGLKMCEPCGGLCPKACEGTGSGSRFQTVDSSNIDGFVNCTKILGNLDFLITGLNGDPWHKIPALDPEKLNVFRTVREITGYLNIQSWPPHMHNFSVFSNLTTIGGRSLYNRGFSLLIMKNLNVTSLGFRSLKEISAGRIYISANRQLCYHHSLNWTKVLRGPTEERLDIKHNRPRRDCVAEGKVCDPLCSSGGCWGPGPGQCLSCRNYSRGGVCVTHCNFLNGEPREFAHEAECFSCHPECQPMEGTATCNGSGSDTCAQCAHFRDGPHCVSSCPHGVLGAKGPIYKYPDVQNECRPCHENCTQGCKGPELQDCLGQTLVLIGKTHLTMALTVIAGLVVIFMMLGGTFLYWRGRRIQNKRAMRRYLERGESIEPLDPSEKANKVLARIFKETELRKLKVLGSGVFGTVHKGVWIPEGESIKIPVCIKVIEDKSGRQSFQAVTDHMLAIGSLDHAHIVRLLGLCPGSSLQLVTQYLPLGSLLDHVRQHRGALGPQLLLNWGVQIAKGMYYLEEHGMVHRNLAARNVLLKSPSQVQVADFGVADLLPPDDKQLLYSEAKTPIKWMALESIHFGKYTHQSDVWSYGVTVWELMTFGAEPYAGLRLAEVPDLLEKGERLAQPQICTIDVYMVMVKCWMIDENIRPTFKELANEFTRMARDPPRYLVIKRESGPGIAPGPEPHGLTNKKLEEVELEPELDLDLDLEAEEDNLATTTLGSALSLPVGTLNRPRGSQSLLSPSSGYMPMNQGNLGESCQESAVSGSSERCPRPVSLHPMPRGCLASESSEGHVTGSEAELQEKVSMCRSRSRSRSPRPRGDSAYHSQRHSLLTPVTPLSPPGLEEEDVNGYVMPDTHLKGTPSSREGTLSSVGLSSVLGTEEEDEDEEYEYMNRRRRHSPPHPPRPSSLEELGYEYMDVGSDLSASLGSTQSCPLHPVPIMPTAGTTPDEDYEYMNRQRDGGGPGGDYAAMGACPASEQGYEEMRAFQGPGHQAPHVHYARLKTLRSLEATDSAFDNPDYWHSRLFPKANAQRT DIP protein P21860 original sequence version: 175 DIP protein P21860 original sequence version: 125 DIP protein P21860 original sequence version: 172 DIP protein P21860 original sequence version: 130 DIP protein P21860 original sequence version: 157 DIP protein P21860 original sequence version: 137 7201E7F66CA374BD ror1_human_gene Human ROR1 gene gene gene human Homo sapiens Human egfr-erbb3 7 15 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait 13 unspecified role unspecified role prey prey association association Fig 4 C/D EGF treatment induced this association. ROR1 knockdown significantly reduced co-immunoprecipitation of EGFR with ERBB3. egfr-erbb3-1 3 13 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait physical association physical association Fig S4A EGF treatment induced this association. ror1-egfr 1 13 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined cysteine_rich_domain-(crd/fz) required to bind necessary binding region required to bind deletion analysis deletion analysis range range range range 10 unspecified role unspecified role prey prey physical association physical association Fig 4E, S4B. erbb3-p85 7 13 unspecified role unspecified role ancillary ancillary 15 unspecified role unspecified role bait bait 14 unspecified role unspecified role bait bait association association Fig 4 F ROR1 knockdown significantly reduced co-immunoprecipitation of p85 with ERBB3. ror1-src 1 13 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey sh3_domain required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig 5C/5F ror1-src-1 3 9 unspecified role unspecified role prey prey 13 unspecified role unspecified role bait bait proline_rich_region required to bind necessary binding region required to bind deletion analysis deletion analysis range range range range physical association physical association Fig 5G src-ror1 7 12 unspecified role unspecified role bait bait 13 unspecified role unspecified role prey prey physical association physical association Fig 5D ror1-src-2 7 12 unspecified role unspecified role prey prey 13 unspecified role unspecified role bait bait physical association physical association Fig 5D, 6C -RHS. ror1-src-4 6 9 enzyme target enzyme target substrate substrate neutral component neutral component P05480:p.Lys297Met mutation mutation certain certain sequence position certain certain certain sequence position certain K M Wed May 02 15:58:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys303met' Tue Jun 30 18:54:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys303Met' Mon Mar 01 10:46:44 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'P05480:p.Lys303Met' mouse-3t3 Mus musculus embryo cells 3t3 Mouse embryo fibroblast cells 13 enzyme enzyme neutral component neutral component Q01973:p.Lys506Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain K A Wed May 02 15:35:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys506ala' Tue Jun 30 15:54:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys506Ala' region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined phosphorylation phosphorylation reaction phosphorylation Fig 5E c-Src phosphorylation. ror1-egfr-1 3 13 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range range range certain certain sequence position certain Q01973:p.Lys506Ala mutation mutation certain certain sequence position certain certain certain sequence position certain K A Wed May 02 19:57:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys506ala' Tue Jun 30 16:19:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys506Ala' 10 unspecified role unspecified role prey prey physical association physical association Fig 6A The interaction between ROR1 and EGFR does not require either ROR1 kinase activity or the c-Src-interacting proline-rich domain of ROR1. EGF egfr-erbb3-2 3 13 unspecified role unspecified role prey prey cysteine_rich_domain-(crd/fz) required to bind necessary binding region required to bind deletion analysis deletion analysis range range range range 10 unspecified role unspecified role bait bait 15 unspecified role unspecified role prey prey association association Fig 6B EGF ror1-egfr-2 7 12 unspecified role unspecified role prey prey 13 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey association association Fig 6C -RHS EGF c-Src knockdown did not cause any appreciable changes in the interaction between ROR1 and EGFR or between EGFR and ERBB3. egfr-erbb3-3 7 13 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait 15 unspecified role unspecified role prey prey association association Fig 6C - LHS EGF c-Src knockdown did not cause any appreciable changes in the interaction between ROR1 and EGFR or between EGFR and ERBB3. nkx2_1-ror1 2 11 unspecified role unspecified role bait bait 16 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig S1B ror1-src-6 5 12 unspecified role unspecified role prey prey 13 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig S5B ror1-src-7 4 12 unspecified role unspecified role prey prey human-nci_h1975 Homo sapiens NCI-H1975 Adenocarcinoma nci_h1975 NCI-H1975 NSCLC - Adenocarcinoma cell line - carries double EGFR mutations L858R and T790M, the latter of which is a well-recognized mutation that confers resistance to EGFR TKI. http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=CRL-5908&Template=cellBiology 13 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined intracellular_domain sufficient to bind sufficient binding region sufficient to bind range range range range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection physical association physical association Fig S5C ror1-src-8 4 12 unspecified role unspecified role prey prey c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 13 unspecified role unspecified role bait bait intracellular_domain sufficient to bind sufficient binding region sufficient to bind range range range range region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction Fig S5D (Human) His-src was expressed in Baculovirus. ror1-src-10 6 13 enzyme enzyme neutral component neutral component intracellular_domain sufficient to bind sufficient binding region sufficient to bind range range range range region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 12 enzyme target enzyme target substrate substrate neutral component neutral component human-nci_h23 Human NCI-H23 adenocarcinoma NSCLC cell line nci_h23 Human NCI-H23 adenocarcinoma NSCLC cell line http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx? ATCCNum=CRL-5800&Template=cellBiology phosphorylation phosphorylation reaction phosphorylation Fig S5E ror1-src-11 6 13 enzyme enzyme neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined intracellular_domain sufficient to bind sufficient binding region sufficient to bind range range range range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 12 enzyme target enzyme target substrate substrate neutral component neutral component human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. phosphorylation phosphorylation reaction phosphorylation Fig S5F src-ror1-1 4 12 unspecified role unspecified role bait bait sh3_domain sufficient to bind sufficient binding region sufficient to bind range range range range n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 13 unspecified role unspecified role prey prey human-nci_h1975 Homo sapiens NCI-H1975 Adenocarcinoma nci_h1975 NCI-H1975 NSCLC - Adenocarcinoma cell line - carries double EGFR mutations L858R and T790M, the latter of which is a well-recognized mutation that confers resistance to EGFR TKI. http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=CRL-5908&Template=cellBiology physical association physical association Fig S5G ror1-egfr-3 7 10 unspecified role unspecified role prey prey 13 unspecified role unspecified role bait bait physical association physical association Fig 6C -RHS EGF c-Src knockdown did not cause any appreciable changes in the interaction between ROR1 and EGFR or between EGFR and ERBB3.