IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Cancer Cell (1535-6108) 2012 imex curation Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. The authors confirmed that all constructs/proteins used were of human origin. ssen@mdanderson.org 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Accepted 2012-MAY-04 by JYOTI 20-Jun-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Cancer Cell (1535-6108) 2012 imex curation Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. The authors confirmed that all constructs/proteins used were of human origin. ssen@mdanderson.org 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Accepted 2012-MAY-04 by JYOTI 20-Jun-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Cancer Cell (1535-6108) 2012 imex curation Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. The authors confirmed that all constructs/proteins used were of human origin. ssen@mdanderson.org 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer The EMSA probe is stated to be a "p21 (CDKN1A) containing the p53 DNA-binding site", but it has not been possible to confirm this. email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Accepted 2012-MAY-04 by JYOTI 20-Jun-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Cancer Cell (1535-6108) 2012 imex curation Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. The authors confirmed that all constructs/proteins used were of human origin. ssen@mdanderson.org 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... GFP tagged proteins were detected by tag fluorescence. Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Accepted 2012-MAY-04 by JYOTI 20-Jun-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Cancer Cell (1535-6108) 2012 imex curation Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. The authors confirmed that all constructs/proteins used were of human origin. ssen@mdanderson.org 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Accepted 2012-MAY-04 by JYOTI 20-Jun-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Cancer Cell (1535-6108) 2012 imex curation Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. The authors confirmed that all constructs/proteins used were of human origin. ssen@mdanderson.org 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions human-panc1 Homo sapiens pancreatic carcinoma cell line PANC-1 panc1 Human pancreatic carcinoma cell line PANC-1 Established from a pancreatic carcinoma of ductal origin from a 56-year-old Caucasian male. Cells possess the type B phenotype for G6PD. fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... GFP tagged proteins were detected by tag fluorescence. Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Accepted 2012-MAY-04 by JYOTI 20-Jun-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Cancer Cell (1535-6108) 2012 imex curation Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. The authors confirmed that all constructs/proteins used were of human origin. ssen@mdanderson.org 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions human-mcf10a Human non-transformed breast epithelial cell line mcf10a non-transformed human breast epithelial cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Accepted 2012-MAY-04 by JYOTI 20-Jun-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Cancer Cell (1535-6108) 2012 imex curation Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. The authors confirmed that all constructs/proteins used were of human origin. ssen@mdanderson.org 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Accepted 2012-MAY-04 by JYOTI Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Cancer Cell (1535-6108) 2012 imex curation Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. The authors confirmed that all constructs/proteins used were of human origin. ssen@mdanderson.org 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... synchronized mitotic cells Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Accepted 2012-MAY-04 by JYOTI 20-Jun-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Cancer Cell (1535-6108) 2012 imex curation Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. The authors confirmed that all constructs/proteins used were of human origin. ssen@mdanderson.org 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... mortalin was detected using an anti-mortalin antibody. Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Accepted 2012-MAY-04 by JYOTI 20-Jun-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Cancer Cell (1535-6108) 2012 imex curation Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. The authors confirmed that all constructs/proteins used were of human origin. ssen@mdanderson.org 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Accepted 2012-MAY-04 by JYOTI 20-Jun-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Cancer Cell (1535-6108) 2012 imex curation Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. The authors confirmed that all constructs/proteins used were of human origin. ssen@mdanderson.org 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Accepted 2012-MAY-04 by JYOTI 20-Jun-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Cancer Cell (1535-6108) 2012 imex curation Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. The authors confirmed that all constructs/proteins used were of human origin. ssen@mdanderson.org 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... Mortalin was detected using an anti-Flag antibody. Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Accepted 2012-MAY-04 by JYOTI 20-Jun-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Cancer Cell (1535-6108) 2012 imex curation Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. The authors confirmed that all constructs/proteins used were of human origin. ssen@mdanderson.org 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. crosslink cross-linking study crosslink anti tag western anti tag western blot anti tag western Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... glutaraldehyde for 15 min Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Accepted 2012-MAY-04 by JYOTI 20-Jun-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Cancer Cell (1535-6108) 2012 imex curation Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. The authors confirmed that all constructs/proteins used were of human origin. ssen@mdanderson.org 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Accepted 2012-MAY-04 by JYOTI 20-Jun-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Cancer Cell (1535-6108) 2012 imex curation Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. The authors confirmed that all constructs/proteins used were of human origin. ssen@mdanderson.org 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel anti tag western anti tag western blot anti tag western Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Accepted 2012-MAY-04 by JYOTI 20-Jun-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Cancer Cell (1535-6108) 2012 imex curation Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. The authors confirmed that all constructs/proteins used were of human origin. ssen@mdanderson.org 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip weight by comassie molecular weight estimation by coomasie staining weight by comassie Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Accepted 2012-MAY-04 by JYOTI 20-Jun-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Cancer Cell (1535-6108) 2012 imex curation Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. The authors confirmed that all constructs/proteins used were of human origin. ssen@mdanderson.org 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions human-saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... GFP tagged proteins were detected by tag-fluorescence. Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Accepted 2012-MAY-04 by JYOTI 20-Jun-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Cancer Cell (1535-6108) 2012 imex curation Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. The authors confirmed that all constructs/proteins used were of human origin. ssen@mdanderson.org 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Accepted 2012-MAY-04 by JYOTI 20-Jun-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Cancer Cell (1535-6108) 2012 imex curation Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. The authors confirmed that all constructs/proteins used were of human origin. ssen@mdanderson.org 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions human-panc1 Homo sapiens pancreatic carcinoma cell line PANC-1 panc1 Human pancreatic carcinoma cell line PANC-1 Established from a pancreatic carcinoma of ductal origin from a 56-year-old Caucasian male. Cells possess the type B phenotype for G6PD. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Accepted 2012-MAY-04 by JYOTI 20-Jun-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Cancer Cell (1535-6108) 2012 imex curation Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. The authors confirmed that all constructs/proteins used were of human origin. ssen@mdanderson.org 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... GFP tagged proteins were detected by tag fluorescence. Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Accepted 2012-MAY-04 by JYOTI 20-Jun-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Cancer Cell (1535-6108) 2012 imex curation Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. The authors confirmed that all constructs/proteins used were of human origin. ssen@mdanderson.org 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... Mad2 was detected by Western Blotting. Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Accepted 2012-MAY-04 by JYOTI 20-Jun-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. Cancer Cell (1535-6108) 2012 imex curation Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. The authors confirmed that all constructs/proteins used were of human origin. ssen@mdanderson.org 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel western blot western blot Immuno blot Immuno blot Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer email sent 28/2 asking for cell line confirmation for figs S3B/C and possible ID for "dominant negative p73 protein ΔNp73" No Reply received - so would you think Figs S3B/C were in HeLa - as Fig S3A? and has the p73 unknown isoform ""dominant negative p73 protein ΔNp73 (Delta Np73)" been captured OK? -multiple isoforms with this designation exist... Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Accepted 2012-MAY-04 by JYOTI 20-Jun-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD DIP protein P04637 original sequence version: 121 DIP protein P04637 original sequence version: 115 DIP protein P04637-1 original sequence version: 224 DIP protein P04637 original sequence version: 117 DIP protein P04637-1 original sequence version: 226 DIP protein P04637 original sequence version: 226 DIP protein P04637 original sequence version: 212 DIP protein P04637 original sequence version: 220 DIP protein P04637-1 original sequence version: 220 DIP protein P04637-1 original sequence version: 145 DIP protein P04637 original sequence version: 146 DIP protein P04637 original sequence version: 145 DIP protein P04637 original sequence version: 166 DIP protein P04637-1 original sequence version: 166 DIP protein P04637 original sequence version: 169 DIP protein P04637 original sequence version: 177 DIP protein P04637 original sequence version: 175 DIP protein P04637-1 original sequence version: 175 DIP protein P04637-1 original sequence version: 172 DIP protein P04637-1 original sequence version: 169 DIP protein P04637 original sequence version: 170 DIP protein P04637 original sequence version: 172 DIP protein P04637-1 original sequence version: 194 DIP protein P04637 original sequence version: 196 DIP protein P04637 original sequence version: 185 DIP protein P04637-1 original sequence version: 191 DIP protein P04637 original sequence version: 191 DIP protein P04637 original sequence version: 180 DIP protein P04637-1 original sequence version: 197 DIP protein P04637 original sequence version: 183 DIP protein P04637-1 original sequence version: 183 DIP protein P04637-1 original sequence version: 185 DIP protein P04637 original sequence version: 199 DIP protein P04637 original sequence version: 201 DIP protein P04637 original sequence version: 189 DIP protein P04637 original sequence version: 235 DIP protein P04637 original sequence version: 230 DIP protein P04637 original sequence version: 234 DIP protein P04637-1 original sequence version: 234 DIP protein P04637-1 original sequence version: 235 DIP protein P04637 original sequence version: 239 DIP protein P04637-1 original sequence version: 212 DIP protein P04637-1 original sequence version: 213 DIP protein P04637 original sequence version: 213 DIP protein P04637 original sequence version: 206 DIP protein P04637-1 original sequence version: 188 DIP protein P04637-1 original sequence version: 228 DIP protein P04637 original sequence version: 208 DIP protein P04637 original sequence version: 240 DIP protein P04637-1 original sequence version: 240 DIP protein P04637 original sequence version: 204 DIP protein P04637-1 original sequence version: 204 DIP protein P04637-1 original sequence version: 209 DIP protein P04637-1 original sequence version: 203 DIP protein P04637 original sequence version: 221 DIP protein P04637-1 original sequence version: 152 DIP protein P04637 original sequence version: 217 DIP protein P04637-1 original sequence version: 140 DIP protein P04637 original sequence version: 215 DIP protein P04637 original sequence version: 214 DIP protein P04637-1 original sequence version: 182 DIP protein P04637 original sequence version: 182 DIP protein P04637 original sequence version: 188 DIP protein P04637 original sequence version: 192 DIP protein P04637-1 original sequence version: 192 DIP protein P04637 original sequence version: 218 DIP protein P04637-1 original sequence version: 218 AD5C149FD8106131 p73_chlae Tumor protein p73 TP73 P73 p53-like transcription factor p53-related protein protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey MAQSTTTSPDGGTTFEHLWSSLEPDSTYFDLPQSSRGNNEVVGGTDSSMDVFHLEGMTTSVMAQFNLLSSTMDQMSSRAASASPYTPEHAASVPTHSPYAQPSSTFDTMSPAPVIPSNTDYPGPHHFEVTFQQSSTAKSATWTYSPLLKKLYCQIAKTCPIQIKVSAPPPPGTAIRAMPVYKKAEHVTDIVKRCPNHELGRDFNEGQSAPASHLIRVEGNNLSQYVDDPVTGRQSVVVPYEPPQVGTEFTTILYNFMCNSSCVGGMNRRPILIIITLETRDGQVLGRRSFEGRICACPGRDRKADEDHYREQQALNESSAKNGAASKRAFKQSPPAVPALGPGVKKRRHGDEDTYYLQVRGRENFEILMKLKESLELMELVPQPLVDSYRQQQQLLQRPSHLQPPSYGPVLSPMNKVHGGVNKLPSVNQLVGQPPPHSSAATPNLGPVGSGMLNNHGHAVPANSEMTSSHGTQSMVSGSHCTPPPPYHADPSLVSFLTGLGCPNCIEYFTSQGLQSIYHLQNLTIEDLGALKIPEQYRMTIWRGLQDLKQGHDYGAAAQQLLRSSNAAAISIGGSGELQRQRVMEAVHFRVRHTITIPNRGGPGAGPDEWADFGFDLPDCKARKQPIKEEFTEAEIH 7CB200B919C9C70A aurka_chlae AURKA_CHLAE protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey aurka_human Aurora kinase A AIRK1 Serine/threonine-protein kinase 15 Aurora/IPL1-related kinase 1 Breast tumor-amplified kinase STK6 AIK ARK1 AURA BTAK STK15 AURKA AYK1 IAK1 Aurora 2 Serine/threonine-protein kinase 6 Serine/threonine-protein kinase aurora-A protein protein human Homo sapiens Human MDRSKENCISGPVKATAPVGGPKRVLVTQQFPCQNPLPVNSGQAQRVLCPSNSSQRVPLQAQKLVSSHKPVQNQKQKQLQATSVPHPVSRPLNNTQKSKQPLPSAPENNPEEELASKQKNEESKKRQWALEDFEIGRPLGKGKFGNVYLAREKQSKFILALKVLFKAQLEKAGVEHQLRREVEIQSHLRHPNILRLYGYFHDATRVYLILEYAPLGTVYRELQKLSKFDEQRTATYITELANALSYCHSKRVIHRDIKPENLLLGSAGELKIADFGWSVHAPSSRRTTLCGTLDYLPPEMIEGRMHDEKVDLWSLGVLCYEFLVGKPPFEANTYQETYKRISRVEFTFPDFVTEGARDLISRLLKHNPSQRPMLREVLEHPWITANSSKPSNCQNKESASKQS DIP protein O14965 original sequence version: 181 DIP protein O14965 original sequence version: 146 DIP protein O14965 original sequence version: 124 DIP protein O14965 original sequence version: 160 DIP protein O14965 original sequence version: 138 DIP protein O14965 original sequence version: 98 125F3594834CD157 o15350-8 Tumor protein p73 TP73 P73 p53-like transcription factor p53-related protein protein protein human Homo sapiens Human MLYVGDPARHLATAQFNLLSSTMDQMSSRAASASPYTPEHAASVPTHSPYAQPSSTFDTMSPAPVIPSNTDYPGPHHFEVTFQQSSTAKSATWTYSPLLKKLYCQIAKTCPIQIKVSTPPPPGTAIRAMPVYKKAEHVTDVVKRCPNHELGRDFNEGQSAPASHLIRVEGNNLSQYVDDPVTGRQSVVVPYEPPQVGTEFTTILYNFMCNSSCVGGMNRRPILIIITLEMRDGQVLGRRSFEGRICACPGRDRKADEDHYREQQALNESSAKNGAASKRAFKQSPPAVPALGAGVKKRRHGDEDTYYLQVRGRENFEILMKLKESLELMELVPQPLVDSYRQQQQLLQRPSHLQPPSYGPVLSPMNKVHGGMNKLPSVNQLVGQPPPHSSAATPNLGPVGPGMLNNHGHAVPANGEMSSSHSAQSMVSGSHCTPPPPYHADPSLVSFLTGLGCPNCIEYFTSQGLQSIYHLQNLTIEDLGALKIPEQYRMTIWRGLQDLKQGHDYSTAQQLLRSSNAATISIGGSGELQRQRVMEAVHFRVRHTITIPNRGGPGGGPDEWADFGFDLPDCKARKQPIKEEFTEAEIH Produced by alternative promoter usage. E8F1CAE88CED4EBC grp75_human Stress-70 protein, mitochondrial HSPA9 GRP75 HSPA9B 75 kDa glucose-regulated protein Heat shock 70 kDa protein 9 Peptide-binding protein 74 Mortalin mt-HSP70 protein protein human Homo sapiens Human MISASRAAAARLVGAAASRGPTAARHQDSWNGLSHEAFRLVSRRDYASEAIKGAVVGIDLGTTNSCVAVMEGKQAKVLENAEGARTTPSVVAFTADGERLVGMPAKRQAVTNPNNTFYATKRLIGRRYDDPEVQKDIKNVPFKIVRASNGDAWVEAHGKLYSPSQIGAFVLMKMKETAENYLGHTAKNAVITVPAYFNDSQRQATKDAGQISGLNVLRVINEPTAAALAYGLDKSEDKVIAVYDLGGGTFDISILEIQKGVFEVKSTNGDTFLGGEDFDQALLRHIVKEFKRETGVDLTKDNMALQRVREAAEKAKCELSSSVQTDINLPYLTMDSSGPKHLNMKLTRAQFEGIVTDLIRRTIAPCQKAMQDAEVSKSDIGEVILVGGMTRMPKVQQTVQDLFGRAPSKAVNPDEAVAIGAAIQGGVLAGDVTDVLLLDVTPLSLGIETLGGVFTKLINRNTTIPTKKSQVFSTAADGQTQVEIKVCQGEREMAGDNKLLGQFTLIGIPPAPRGVPQIEVTFDIDANGIVHVSAKDKGTGREQQIVIQSSGGLSKDDIENMVKNAEKYAEEDRRKKERVEAVNMAEGIIHDTETKMEEFKDQLPADECNKLKEEISKMRELLARKDSETGENIRQAASSLQQASLKLFEMAYKKMASEREGSGSSGTGEQKEDQKEEKQ DIP protein P38646 original sequence version: 188 90969A8D06757753 grp75_chlae HSPA9/Mortalin protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey cdc20_human Cell division cycle protein 20 homolog CDC20 p55CDC protein protein human Homo sapiens Human MAQFAFESDLHSLLQLDAPIPNAPPARWQRKAKEAAGPAPSPMRAANRSHSAGRTPGRTPGKSSSKVQTTPSKPGGDRYIPHRSAAQMEVASFLLSKENQPENSQTPTKKEHQKAWALNLNGFDVEEAKILRLSGKPQNAPEGYQNRLKVLYSQKATPGSSRKTCRYIPSLPDRILDAPEIRNDYYLNLVDWSSGNVLAVALDNSVYLWSASSGDILQLLQMEQPGEYISSVAWIKEGNYLAVGTSSAEVQLWDVQQQKRLRNMTSHSARVGSLSWNSYILSSGSRSGHIHHHDVRVAEHHVATLSGHSQEVCGLRWAPDGRHLASGGNDNLVNVWPSAPGEGGWVPLQTFTQHQGAVKAVAWCPWQSNVLATGGGTSDRHIRIWNVCSGACLSAVDAHSQVCSILWSPHYKELISGHGFAQNQLVIWKYPTMAKVAELKGHTSRVLSLTMSPDGATVASAAADETLRLWRCFELDPARRREREKASAAKSSLIHQGIR DIP protein Q12834 original sequence version: 132 DIP protein Q12834 original sequence version: 74 DIP protein Q12834 original sequence version: 100 DIP protein Q12834 original sequence version: 118 DIP protein Q12834 original sequence version: 125 DIP protein Q12834 original sequence version: 114 DIP protein Q12834 original sequence version: 147 DIP protein Q12834 original sequence version: 162 FD5C967AF84089E8 p73_human Tumor protein p73 TP73 P73 p53-like transcription factor p53-related protein protein protein human Homo sapiens Human MAQSTATSPDGGTTFEHLWSSLEPDSTYFDLPQSSRGNNEVVGGTDSSMDVFHLEGMTTSVMAQFNLLSSTMDQMSSRAASASPYTPEHAASVPTHSPYAQPSSTFDTMSPAPVIPSNTDYPGPHHFEVTFQQSSTAKSATWTYSPLLKKLYCQIAKTCPIQIKVSTPPPPGTAIRAMPVYKKAEHVTDVVKRCPNHELGRDFNEGQSAPASHLIRVEGNNLSQYVDDPVTGRQSVVVPYEPPQVGTEFTTILYNFMCNSSCVGGMNRRPILIIITLEMRDGQVLGRRSFEGRICACPGRDRKADEDHYREQQALNESSAKNGAASKRAFKQSPPAVPALGAGVKKRRHGDEDTYYLQVRGRENFEILMKLKESLELMELVPQPLVDSYRQQQQLLQRPSHLQPPSYGPVLSPMNKVHGGMNKLPSVNQLVGQPPPHSSAATPNLGPVGPGMLNNHGHAVPANGEMSSSHSAQSMVSGSHCTPPPPYHADPSLVSFLTGLGCPNCIEYFTSQGLQSIYHLQNLTIEDLGALKIPEQYRMTIWRGLQDLKQGHDYSTAQQLLRSSNAATISIGGSGELQRQRVMEAVHFRVRHTITIPNRGGPGGGPDEWADFGFDLPDCKARKQPIKEEFTEAEIH DIP protein O15350 original sequence version: 170 DIP protein O15350-1 original sequence version: 100 DIP protein O15350 original sequence version: 123 DIP protein O15350 original sequence version: 149 DIP protein O15350-1 original sequence version: 165 DIP protein O15350 original sequence version: 92 DIP protein O15350-1 original sequence version: 122 DIP protein O15350-1 original sequence version: 135 DIP protein O15350-1 original sequence version: 174 A467493C5D93EEE0 p53/p73_binding _consensus_site_probe probe containing the p53 DNA-binding site probe dna deoxyribonucleic acid DNA deoxyribonucleic acid dna DNA deoxyribonucleic acid chemical synthesis Chemical synthesis (Chemical synthesis) TACAGAACATGTCTAAGCATGCTGGGG md2l1_human Mitotic spindle assembly checkpoint protein MAD2A MAD2L1 MAD2 Mitotic arrest deficient 2-like protein 1 protein protein human Homo sapiens Human MALQLSREQGITLRGSAEIVAEFFSFGINSILYQRGIYPSETFTRVQKYGLTLLVTTDLELIKYLNNVVEQLKDWLYKCSVQKLVVVISNIESGEVLERWQFDIECDKTAKDDSAPREKSQKAIQDEIRSVIRQITATVTFLPLLEVSCSFDLLIYTDKDLVVPEKWEESGPQFITNSEEVRLRSFTTTIHKVNSMVAYKIPVND DIP protein Q13257 original sequence version: 133 DIP protein Q13257 original sequence version: 78 DIP protein Q13257 original sequence version: 105 DIP protein Q13257 original sequence version: 120 DIP protein Q13257 original sequence version: 127 DIP protein Q13257 original sequence version: 116 DIP protein Q13257 original sequence version: 150 DIP protein Q13257 original sequence version: 156 DIP protein Q13257 original sequence version: 134 B8DCBF0043836764 aurka-tp73 8 27 enzyme enzyme neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O14965:p.Lys162Arg mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain K R Wed May 02 18:28:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys162arg' Tue Jun 30 17:40:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys162Arg' 32 enzyme target enzyme target substrate substrate neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O15350:p.Ser235Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain S A Wed May 02 19:26:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser235ala' Tue Jun 30 19:15:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser235Ala' phosphorylation phosphorylation reaction phosphorylation Fig 1E, 1F, S1A p73 is phosphorylated by Aurora-A. The N terminally truncated isoform Delta Np73 showed minimal loss of phosphorylation in the SA mutant. aurka-tp73-1 11 27 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 32 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 1H aurka-tp73-2 9 25 unspecified role unspecified role bait bait 26 unspecified role unspecified role prey prey physical association physical association Fig 1I,1J,1K. The amount of Aurora-A bound to p73 progressively increased, peaking at mitosis. tp73-grp75-2 9 30 unspecified role unspecified role prey prey 25 unspecified role unspecified role bait bait physical association physical association Fig 4D p53-aurka 3 33 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 32 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O15350:p.Ser235Asp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain S D Wed May 02 15:21:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser235asp' Tue Jun 30 19:14:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser235Asp' O15350:p.Ser235Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S A Wed May 02 17:03:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser235ala' Tue Jun 30 20:13:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser235Ala' in vitro In vitro direct interaction direct interaction Fig 2A tp73-1 14 32 putative self putative self putative self putative self region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 3D aurka-tp73-3 4 25 unspecified role unspecified role neutral component neutral component 27 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig 3E, 3F. Cells overexpressing Aurora-A showed evenly diffused endogenous p73 staining in the cytoplasm and nucleus, which were reversed with Aurora-A inhibitor MLN8054. tp73-hspa9 17 32 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O15350:p.Ser235Asp mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain S D Wed May 02 14:26:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser235asp' Tue Jun 30 17:54:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser235Asp' 29 unspecified role unspecified role prey prey physical association physical association Fig 4A A protein band of approximately 80 kD MW was detected only in the immune complex of the S235D mutant but not the WT. tp73-hspa9-1 15 32 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O15350:p.Ser235Asp mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain S D Wed May 02 14:49:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser235asp' Tue Jun 30 19:26:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser235Asp' 29 unspecified role unspecified role neutral component neutral component physical association physical association Fig 4B The S235D mutant and mortalin-containing complexes were significantly more enriched at >2-megadalton-sized fractions than were the p73-WT and mortalin complexes. tp73-hspa9-2 1 29 unspecified role unspecified role bait bait p53_binding_domain required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 32 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O15350:p.Ser235Asp mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain S D Wed May 02 16:01:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser235asp' Tue Jun 30 18:49:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser235Asp' physical association physical association Fig 4C WT and mutant p73 did not interact with the mortalin deletion mutant, but full-length mortalin's interaction was enhanced with S235D. tp73-grp75-1 9 30 unspecified role unspecified role prey prey 25 unspecified role unspecified role bait bait physical association physical association Fig 4E tp73-aurka-9 10 27 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 25 unspecified role unspecified role bait bait 30 unspecified role unspecified role prey prey association association Fig 4E Less mortalin bound to p73 in Aurora-A inhibitor MLN8054 treated cells, than in untreated cells. MLN8054 tp73-aurka-1 5 29 unspecified role unspecified role prey prey 32 unspecified role unspecified role bait bait 27 unspecified role unspecified role prey prey association association Fig 4F Less mortalin bound to p73 in Aurora-A inhibitor MLN8054 treated cells, than in untreated cells. MLN8054 tp73-aurka-2 20 32 unspecified role unspecified role bait bait 29 unspecified role unspecified role prey prey 27 unspecified role unspecified role prey prey association association Fig 4F Less mortalin bound to p73 in Aurora-A inhibitor MLN8054 treated cells, than in untreated cells. MLN8054 aurka-tp73-4 4 29 unspecified role unspecified role neutral component neutral component p53_binding_domain required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 32 unspecified role unspecified role neutral component neutral component O15350:p.Ser235Asp mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain S D Wed May 02 16:11:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser235asp' Tue Jun 30 15:42:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser235Asp' region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Fig 5A/B In cells with mutant mortalin, the p73 S235D mutant translocated into the nucleus more than in the empty-vector-transfected cells hspa9-tp73-2 21 29 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined p53_binding_domain required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 32 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig 5F Nuclear staining was detected in 36% of mortalin mutant MCF-7 and Panc-1 cells (n = 100) versus 2% of empty vector cells. hspa9-tp73-3 6 32 unspecified role unspecified role neutral component neutral component 29 unspecified role unspecified role neutral component neutral component p53_binding_domain required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Fig 5F Nuclear staining was detected in 36% of mortalin mutant MCF-7 and Panc-1 cells (n = 100) versus 2% of empty vector cells. tp73-aurka-4 2 34 unspecified role unspecified role prey prey 27 unspecified role unspecified role prey prey 32 unspecified role unspecified role bait bait 31 unspecified role unspecified role prey prey association association Fig 7E tp73-aurka-5 2 32 unspecified role unspecified role prey prey 31 unspecified role unspecified role bait bait 27 unspecified role unspecified role prey prey 34 unspecified role unspecified role prey prey association association Fig 7E tp73-aurka-6 22 32 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O15350:p.Ser235Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S D Wed May 02 15:20:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser235asp' Tue Jun 30 16:54:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser235Asp' 34 unspecified role unspecified role prey prey 31 unspecified role unspecified role bait bait association association Fig 7F There was a marked reduction in the interaction of both S235D mutant and MAD2 with CDC20, compared with that in empty vector and S235A mutant cells. tp73-aurka-7 18 32 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 34 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig 7G tp73-tp73-3 12 28 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 32 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association S1B Isoform Delta Np73 appeared to bind the WT and the phosphor-mimetic (S235D) mutant of p73 with similar efficiency. aurka-tp73-7 11 27 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 32 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O15350:p.Ser235Asp mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain S D Wed May 02 15:21:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser235asp' Tue Jun 30 17:34:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser235Asp' physical association physical association Fig S1C aurka-tp73-8 7 32 unspecified role unspecified role prey prey 27 unspecified role unspecified role bait bait physical association physical association Fig S1D aurka-tp73-9 7 32 unspecified role unspecified role bait bait 27 unspecified role unspecified role prey prey physical association physical association Fig S1D aurka-tp73-10 19 27 unspecified role unspecified role prey prey 32 unspecified role unspecified role bait bait physical association physical association Fig S1E aurka-tp73-10 19 27 unspecified role unspecified role bait bait 32 unspecified role unspecified role prey prey physical association physical association Fig S1E tp73-tp73-1 16 32 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O15350:p.Ser235Asp mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain S D Wed May 02 16:13:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser235asp' Tue Jun 30 19:18:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser235Asp' 32 unspecified role unspecified role neutral component neutral component O15350:p.Ser235Asp mutation mutation certain certain sequence position certain certain certain sequence position certain S D Wed May 02 18:18:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser235asp' Tue Jun 30 16:52:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser235Asp' region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association S3A -LHS Enrichment of S235D mutant and mortalin in the higher molecular complex was also evident. hspa9-tp73 23 32 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 29 unspecified role unspecified role neutral component neutral component physical association physical association S3A -RHS Enrichment of S235D mutant and mortalin in the higher molecular complex was also evident. hspa9-tp73-1 23 32 unspecified role unspecified role neutral component neutral component O15350:p.Ser235Asp mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain S D Wed May 02 17:59:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser235asp' Tue Jun 30 17:15:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser235Asp' region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 29 unspecified role unspecified role neutral component neutral component physical association physical association S3A -RHS Enrichment of S235D mutant and mortalin in the higher molecular complex was also evident. tp73-1 16 32 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association S3A -LHS Enrichment of S235D mutant and mortalin in the higher molecular complex was also evident. tp73-tp73-2 16 32 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 32 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association S3A LHS tp73-3 16 32 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O15350:p.Ser235Asp mutation mutation certain certain sequence position certain certain certain sequence position certain S D Wed May 02 17:04:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser235asp' Tue Jun 30 18:45:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser235Asp' physical association physical association S3A -LHS mmm Enrichment of S235D mutant and mortalin in the higher molecular complex was also evident. hspa9-tp53-1 1 29 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined p53_binding_domain required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 24 unspecified role unspecified role prey prey P04637:p.Ser215Asp mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain S D Wed May 02 17:50:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser215asp' Tue Jun 30 18:23:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser215Asp' region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig S3B WT and mutant p53 did not interact with the mortalin deletion mutant, but full-length mortalin's interaction was enhanced with S215D. tp73-aurka-8 13 27 unspecified role unspecified role prey prey 29 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined p53_binding_domain required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 32 unspecified role unspecified role bait bait association association Fig S3C Less mortalin bound to p73 in Aurora-A inhibitor MLN8054 treated cells, than in untreated cells. MLN8054