Molecular Connections Molecular Connections Pvt. Ltd. 2/2, Kariappa Road, Basavanagudi, Bangalore -560 004 www.molecularconnections.com Brain-derived neurotrophic factor receptor TrkB exists as a preformed dimer in living cells. Brain-derived neurotrophic factor receptor TrkB exists as a preformed dimer in living cells. J Mol Signal 2012 imex curation Shen J., Maruyama I.N. ichi@oist.jp Only protein-protein interactions Molecular Connections Pvt. Ltd. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. split firefly luciferase complementation split firefly luciferase complementation predetermined predetermined participant predetermined Shen J., Maruyama I.N. ichi@oist.jp Accepted 2012-MAY-28 by PPORRAS J Mol Signal 2012 Only protein-protein interactions imex curation Molecular Connections Pvt. Ltd. Brain-derived neurotrophic factor receptor TrkB exists as a preformed dimer in living cells. Brain-derived neurotrophic factor receptor TrkB exists as a preformed dimer in living cells. J Mol Signal 2012 imex curation Shen J., Maruyama I.N. ichi@oist.jp Only protein-protein interactions Molecular Connections Pvt. Ltd. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. bifc bimolecular fluorescence complementation bifc predetermined predetermined participant predetermined Shen J., Maruyama I.N. ichi@oist.jp Accepted 2012-MAY-28 by PPORRAS J Mol Signal 2012 Only protein-protein interactions imex curation Molecular Connections Pvt. Ltd. ntrk2_human BDNF/NT-3 growth factors receptor Tropomyosin-related kinase B NTRK2 TRKB GP145-TrkB Neurotrophic tyrosine kinase receptor type 2 TrkB tyrosine kinase protein protein human Homo sapiens Human MSSWIRWHGPAMARLWGFCWLVVGFWRAAFACPTSCKCSASRIWCSDPSPGIVAFPRLEPNSVDPENITEIFIANQKRLEIINEDDVEAYVGLRNLTIVDSGLKFVAHKAFLKNSNLQHINFTRNKLTSLSRKHFRHLDLSELILVGNPFTCSCDIMWIKTLQEAKSSPDTQDLYCLNESSKNIPLANLQIPNCGLPSANLAAPNLTVEEGKSITLSCSVAGDPVPNMYWDVGNLVSKHMNETSHTQGSLRITNISSDDSGKQISCVAENLVGEDQDSVNLTVHFAPTITFLESPTSDHHWCIPFTVKGNPKPALQWFYNGAILNESKYICTKIHVTNHTEYHGCLQLDNPTHMNNGDYTLIAKNEYGKDEKQISAHFMGWPGIDDGANPNYPDVIYEDYGTAANDIGDTTNRSNEIPSTDVTDKTGREHLSVYAVVVIASVVGFCLLVMLFLLKLARHSKFGMKGPASVISNDDDSASPLHHISNGSNTPSSSEGGPDAVIIGMTKIPVIENPQYFGITNSQLKPDTFVQHIKRHNIVLKRELGEGAFGKVFLAECYNLCPEQDKILVAVKTLKDASDNARKDFHREAELLTNLQHEHIVKFYGVCVEGDPLIMVFEYMKHGDLNKFLRAHGPDAVLMAEGNPPTELTQSQMLHIAQQIAAGMVYLASQHFVHRDLATRNCLVGENLLVKIGDFGMSRDVYSTDYYRVGGHTMLPIRWMPPESIMYRKFTTESDVWSLGVVLWEIFTYGKQPWYQLSNNEVIECITQGRVLQRPRTCPQEVYELMLGCWQREPHMRKNIKGIHTLLQNLAKASPVYLDILG DIP protein Q16620 original sequence version: 83 DIP protein Q16620 original sequence version: 129 DIP protein Q16620 original sequence version: 151 2FEB9159948F0D13 ntrk2-ntrk2-1 2 3 unspecified role unspecified role neutral component neutral component intracellular domain required to bind necessary binding region required to bind mutation analysis mutation analysis range range range range c-term venus-c venus c-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range N-terminal fragment of the Venus fluorescent protein 3 unspecified role unspecified role neutral component neutral component c-term venus-c venus c-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range C-terminal fragment of the Venus fluorescent protein intracellular domain required to bind necessary binding region required to bind mutation analysis mutation analysis range range range range physical association physical association fig. 2a, 2b, 2c, 2d ntrk2-ntrk2-2 1 3 unspecified role unspecified role unspecified role unspecified role intracellular domain required to bind necessary binding region required to bind mutation analysis mutation analysis range range range range c-firefly tag cfire C-terminal firefly luciferase tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 3 unspecified role unspecified role unspecified role unspecified role intracellular domain required to bind necessary binding region required to bind mutation analysis mutation analysis range range range range n-firefly tag nfire N-terminal fragment of firefly luciferase c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association fig. 3a, b and c