IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Solution structure analysis of the cytoplasmic domain of podocyte protein NEPH1 using SWAXS (Small/Wide Angle X-ray Scattering). Solution structure analysis of the cytoplasmic domain of podocyte protein NEPH1 using SWAXS (Small/Wide Angle X-ray Scattering). J. Biol. Chem. (0021-9258) 2012 imex curation Mallik L., Arif E., Sharma P., Rathore Y.S., Wong H.N., Holzman L.B., Ashish A., Nihalani D. deepakn@mail.med.upenn.edu,ashgang@imtech.res.in 03-Dec-2015: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Mallik L., Arif E., Sharma P., Rathore Y.S., Wong H.N., Holzman L.B., Ashish A., Nihalani D. deepakn@mail.med.upenn.edu,ashgang@imtech.res.in deepakn@mail.med.upenn.edudeepakn@mail.med.upenn.edu Accepted 2015-NOV-20 AT 11:36 GMT AT 11:36 GMT by ORCHARD J. Biol. Chem. (0021-9258) 2012 imex curation Solution structure analysis of the cytoplasmic domain of podocyte protein NEPH1 using SWAXS (Small/Wide Angle X-ray Scattering). Solution structure analysis of the cytoplasmic domain of podocyte protein NEPH1 using SWAXS (Small/Wide Angle X-ray Scattering). J. Biol. Chem. (0021-9258) 2012 imex curation Mallik L., Arif E., Sharma P., Rathore Y.S., Wong H.N., Holzman L.B., Ashish A., Nihalani D. deepakn@mail.med.upenn.edu,ashgang@imtech.res.in 03-Dec-2015: Contacted by IntAct-Help. Only protein-protein interactions chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Mallik L., Arif E., Sharma P., Rathore Y.S., Wong H.N., Holzman L.B., Ashish A., Nihalani D. deepakn@mail.med.upenn.edu,ashgang@imtech.res.in deepakn@mail.med.upenn.edudeepakn@mail.med.upenn.edu Accepted 2015-NOV-20 AT 11:36 GMT AT 11:36 GMT by ORCHARD J. Biol. Chem. (0021-9258) 2012 imex curation Solution structure analysis of the cytoplasmic domain of podocyte protein NEPH1 using SWAXS (Small/Wide Angle X-ray Scattering). Solution structure analysis of the cytoplasmic domain of podocyte protein NEPH1 using SWAXS (Small/Wide Angle X-ray Scattering). J. Biol. Chem. (0021-9258) 2012 imex curation Mallik L., Arif E., Sharma P., Rathore Y.S., Wong H.N., Holzman L.B., Ashish A., Nihalani D. deepakn@mail.med.upenn.edu,ashgang@imtech.res.in 03-Dec-2015: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro saxs x ray scattering saxs predetermined predetermined participant predetermined Mallik L., Arif E., Sharma P., Rathore Y.S., Wong H.N., Holzman L.B., Ashish A., Nihalani D. deepakn@mail.med.upenn.edudeepakn@mail.med.upenn.edu deepakn@mail.med.upenn.edu,ashgang@imtech.res.in Accepted 2015-NOV-20 AT 11:36 GMT AT 11:36 GMT by ORCHARD J. Biol. Chem. (0021-9258) 2012 imex curation Solution structure analysis of the cytoplasmic domain of podocyte protein NEPH1 using SWAXS (Small/Wide Angle X-ray Scattering). Solution structure analysis of the cytoplasmic domain of podocyte protein NEPH1 using SWAXS (Small/Wide Angle X-ray Scattering). J. Biol. Chem. (0021-9258) 2012 imex curation Mallik L., Arif E., Sharma P., Rathore Y.S., Wong H.N., Holzman L.B., Ashish A., Nihalani D. deepakn@mail.med.upenn.edu,ashgang@imtech.res.in 03-Dec-2015: Contacted by IntAct-Help. Only protein-protein interactions chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Mallik L., Arif E., Sharma P., Rathore Y.S., Wong H.N., Holzman L.B., Ashish A., Nihalani D. deepakn@mail.med.upenn.edu,ashgang@imtech.res.in deepakn@mail.med.upenn.edudeepakn@mail.med.upenn.edu Accepted 2015-NOV-20 AT 11:36 GMT AT 11:36 GMT by ORCHARD J. Biol. Chem. (0021-9258) 2012 imex curation Solution structure analysis of the cytoplasmic domain of podocyte protein NEPH1 using SWAXS (Small/Wide Angle X-ray Scattering). Solution structure analysis of the cytoplasmic domain of podocyte protein NEPH1 using SWAXS (Small/Wide Angle X-ray Scattering). J. Biol. Chem. (0021-9258) 2012 imex curation Mallik L., Arif E., Sharma P., Rathore Y.S., Wong H.N., Holzman L.B., Ashish A., Nihalani D. deepakn@mail.med.upenn.edu,ashgang@imtech.res.in 03-Dec-2015: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Mallik L., Arif E., Sharma P., Rathore Y.S., Wong H.N., Holzman L.B., Ashish A., Nihalani D. deepakn@mail.med.upenn.edu,ashgang@imtech.res.in deepakn@mail.med.upenn.edudeepakn@mail.med.upenn.edu Accepted 2015-NOV-20 AT 11:36 GMT AT 11:36 GMT by ORCHARD J. Biol. Chem. (0021-9258) 2012 imex curation kirr1_human Kin of IRRE-like protein 1 KIRREL1 KIRREL Kin of irregular chiasm-like protein 1 NEPH1 Nephrin-like protein 1 protein protein human Homo sapiens Human MLSLLVWILTLSDTFSQGTQTRFSQEPADQTVVAGQRAVLPCVLLNYSGIVQWTKDGLALGMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWFRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIELDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPEILGYRWAKGGFLIEDAHESRYETNVDYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDIGSDVTLTCVWVGNPPLTLTWTKKDSNMVLSNSNQLLLKSVTQADAGTYTCRAIVPRIGVAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLERYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVLPVGIIAGATIGASILLIFFFIALVFFLYRRRKGSRKDVTLRKLDIKVETVNREPLTMHSDREDDTASVSTATRVMKAIYSSFKDDVDLKQDLRCDTIDTREEYEMKDPTNGYYNVRAHEDRPSSRAVLYADYRAPGPARFDGRPSSRLSHSSGYAQLNTYSRGPASDYGPEPTPPGPAAPAGTDTTSQLSYENYEKFNSHPFPGAAGYPTYRLGYPQAPPSGLERTPYEAYDPIGKYATATRFSYTSQHSDYGQRFQQRMQTHV B937421970447FC2 zo1_human Tight junction protein ZO-1 TJP1 ZO1 Zonula occludens protein 1 Zona occludens protein 1 Tight junction protein 1 protein protein human Homo sapiens Human MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF 189E31617084C0CC kirrel-tjp1-1 3 7 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range pdz region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role neutral component neutral component c-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 11 13 direct interaction direct interaction Table 2, Table 3 tjp1-kirrel-1 2 7 unspecified role unspecified role bait bait n-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 6 unspecified role unspecified role prey prey Q96J84:p.Lys729Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K A Wed May 02 18:55:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys729ala' Tue Jun 30 15:49:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys729Ala' n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q96J84:p.Tyr730Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Y A Wed May 02 18:14:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr730ala' Tue Jun 30 18:30:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr730Ala' physical association physical association Fig. 6B kirrel-tjp1-2 4 7 unspecified role unspecified role prey prey n-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role bait bait c-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q96J84:p.Lys729Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K A Wed May 02 19:31:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys729ala' Tue Jun 30 18:18:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys729Ala' n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q96J84:p.Tyr730Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Y A Wed May 02 14:59:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr730ala' Tue Jun 30 16:53:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr730Ala' 25 27 physical association physical association Fig. 6B kirrel-tjp1-3 1 7 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range pdz region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role neutral component neutral component c-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 36 34 direct interaction direct interaction Supp Figs. 3, 4 kirrel-tjp1-4 5 7 unspecified role unspecified role prey prey n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role bait bait c-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 41 43 direct interaction direct interaction Supp. Fig. 7 kirrel-tjp1-5 5 7 unspecified role unspecified role prey prey n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role bait bait n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 48 51 direct interaction direct interaction Supp. Fig. 7 kirrel-tjp1-6 5 7 unspecified role unspecified role prey prey n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role bait bait c-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 57 55 direct interaction direct interaction Supp. Fig. 7