matrixdb Institut de Biologie et de Chimie des Prot\u00c3\u00a9ines, 7, passage du Vercors 69 367 Lyon cedex 07 FRANCE matrixdb@ibcp.fr http://matrixdb.ibcp.fr A comprehensive model of hyaluronan turnover in the mouse. A comprehensive model of hyaluronan turnover in the mouse. Matrix Biol. (0945-053X) 2012 imex curation Jadin L., Bookbinder L.H., Frost G.I. ljadin@halozyme.com 19-Sept-2012: Contacted by IntAct-Help. in vitro In vitro cleavage cleavage assay cleavage tag fluorescence tag visualisation by fluorescence tag fluorescence Jadin L., Bookbinder L.H., Frost G.I. ljadin@halozyme.com Accepted 2012-AUG-02 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. Matrix Biol. (0945-053X) 2012 imex curation A comprehensive model of hyaluronan turnover in the mouse. A comprehensive model of hyaluronan turnover in the mouse. Matrix Biol. (0945-053X) 2012 imex curation Jadin L., Bookbinder L.H., Frost G.I. ljadin@halozyme.com 19-Sept-2012: Contacted by IntAct-Help. in vitro In vitro cleavage cleavage assay cleavage weight by staining molecular weight estimation by staining weight by staining Jadin L., Bookbinder L.H., Frost G.I. ljadin@halozyme.com Accepted 2012-AUG-02 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. Matrix Biol. (0945-053X) 2012 imex curation A comprehensive model of hyaluronan turnover in the mouse. A comprehensive model of hyaluronan turnover in the mouse. Matrix Biol. (0945-053X) 2012 imex curation Jadin L., Bookbinder L.H., Frost G.I. ljadin@halozyme.com 19-Sept-2012: Contacted by IntAct-Help. in vitro In vitro cleavage cleavage assay cleavage weight identificat confirmation by molecular weight weight identificat Jadin L., Bookbinder L.H., Frost G.I. ljadin@halozyme.com Accepted 2012-AUG-02 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. Matrix Biol. (0945-053X) 2012 imex curation A comprehensive model of hyaluronan turnover in the mouse. A comprehensive model of hyaluronan turnover in the mouse. Matrix Biol. (0945-053X) 2012 imex curation Jadin L., Bookbinder L.H., Frost G.I. ljadin@halozyme.com 19-Sept-2012: Contacted by IntAct-Help. in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa tag perox activity tag visualisation by peroxidase activity tag perox activity Jadin L., Bookbinder L.H., Frost G.I. ljadin@halozyme.com Accepted 2012-AUG-02 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. Matrix Biol. (0945-053X) 2012 imex curation hyalp_human Hyaluronidase PH-20 SPAM1 HYAL3 PH20 Hyaluronoglucosaminidase PH-20 Sperm adhesion molecule 1 Sperm surface protein PH-20 protein protein human Homo sapiens Human MGVLKFKHIFFRSFVKSSGVSQIVFTFLLIPCCLTLNFRAPPVIPNVPFLWAWNAPSEFCLGKFDEPLDMSLFSFIGSPRINATGQGVTIFYVDRLGYYPYIDSITGVTVNGGIPQKISLQDHLDKAKKDITFYMPVDNLGMAVIDWEEWRPTWARNWKPKDVYKNRSIELVQQQNVQLSLTEATEKAKQEFEKAGKDFLVETIKLGKLLRPNHLWGYYLFPDCYNHHYKKPGYNGSCFNVEIKRNDDLSWLWNESTALYPSIYLNTQQSPVAATLYVRNRVREAIRVSKIPDAKSPLPVFAYTRIVFTDQVLKFLSQDELVYTFGETVALGASGIVIWGTLSIMRSMKSCLLLDNYMETILNPYIINVTLAAKMCSQVLCQEQGVCIRKNWNSSDYLHLNPDNFAIQLEKGGKFTVRGKPTLEDLEQFSEKFYCSCYSTLSCKEKADVKDTDAVDVCIADGVCIDAFLKPPMETEEPQIFYNASPSTLSATMFIVSILFLIISSVASL 5ADB4739747E32E8 hyaluronan Hyaluronan small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) habp2_bovin Hyaluronan-binding protein 2 HABP2 protein protein bovin Bos taurus (Bovine) Bovine MFARMSDLHVLLLMVLAGKTAFGLSLLSFLTEPDPDWTPDQYEYSQEYNNQEENASSTTAYSDNPDWYYEEDDPCLSNPCTHGGDCLVSGATFTCRCPDPFSGNRCQNVQNKCKNNPCGRGDCLITQSPPYHRCACKHPYRGSDCSRVVPVCRPNPCQNGGTCSRQRRRSKFTCACPDQFKGKLCEIGPDDCYVDDGYSYRGRVSKTIHQHTCLYWNSHLLLQEKYNMFMEDAEAHGIGEHNFCRNPDGDKKPWCFIKVNNVKVKWEYCDVPACSALDVANPEGRPTEPLTKFPEFGSCGRTEIAEKKVKRIFGGFKSTAGKHPWQASLQTSLRLTVSTPQGHYCGGALIHPCWVLTAAHCTEIKTKYLKVVLGDQDLTKTEFHEQSFGVQKIFKYSHYIEIDDIPYNDIALLKLKPVDGHCALESKYVKTVCLPDGPFLSGTECYISGWGVTETGEGSRHLLDAKVKLISNTICNSRQLYDHSIDDNMICAGNLQKPGQDSCQGDSGGPLTCEKDGTSYIYGIVSWGLECGKRPGVYTQVTKFLTWIKATMEKEASF EB9C40217B9C94A0 hyaluronan-spam1 1 6 enzyme target enzyme target substrate substrate neutral component neutral component tag fluorescein fluorescein label fluorescein feature detection feature detection method feature detection undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9bact Bacteria 5 enzyme enzyme neutral component neutral component crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. cleavage cleavage reaction cleavage 1A, suppl Fig2 hyaluronan-spam1-2 2 5 enzyme enzyme neutral component neutral component crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 6 enzyme target enzyme target substrate substrate neutral component neutral component tag fluorescein fluorescein label fluorescein predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9bact Bacteria cleavage cleavage reaction cleavage 1B, suppl Fig2 hyaluronan-spam1-3 3 6 enzyme target enzyme target substrate substrate neutral component neutral component tag fluorescein fluorescein label fluorescein predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9bact Bacteria 5 enzyme enzyme neutral component neutral component crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. cleavage cleavage reaction cleavage 1C hyaluronan-spam1-6 2 5 enzyme enzyme neutral component neutral component 9bact Bacteria 6 enzyme target enzyme target substrate substrate neutral component neutral component crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. cleavage cleavage reaction cleavage Suppl Fig2B habp2-hyaluronan 4 7 unspecified role unspecified role bait bait bovin-cartilage Bos taurus cartilage Cartilage 6 unspecified role unspecified role prey prey biotin biotin tag biotin tag feature detection feature detection method feature detection undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9bact Bacteria 6 competitor competitor competitor neutral component neutral component 9bact Bacteria direct interaction direct interaction Suppl Fig 1 habp2-hyaluronan-2 4 7 unspecified role unspecified role bait bait bovin-cartilage Bos taurus cartilage Cartilage 6 unspecified role unspecified role prey prey biotin biotin tag biotin tag feature detection feature detection method feature detection undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9bact Bacteria 6 competitor competitor competitor neutral component neutral component tag fluorescein fluorescein label fluorescein predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9bact Bacteria direct interaction direct interaction Suppl Fig 1