mpidb The Microbial Protein Interaction Database Substrate recognition mechanism and substrate-dependent conformational changes of an ROK family glucokinase from Streptomyces griseus. Substrate recognition mechanism and substrate-dependent conformational changes of an ROK family glucokinase from Streptomyces griseus. J. Bacteriol. (0021-9193) 2012 imex curation Miyazono K., Tabei N., Morita S., Ohnishi Y., Horinouchi S., Tanokura M. 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Miyazono K., Tabei N., Morita S., Ohnishi Y., Horinouchi S., Tanokura M. Accepted 2012-DEC-06 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. J. Bacteriol. (0021-9193) 2012 Only protein-protein interactions imex curation Substrate recognition mechanism and substrate-dependent conformational changes of an ROK family glucokinase from Streptomyces griseus. Substrate recognition mechanism and substrate-dependent conformational changes of an ROK family glucokinase from Streptomyces griseus. J. Bacteriol. (0021-9193) 2012 imex curation Miyazono K., Tabei N., Morita S., Ohnishi Y., Horinouchi S., Tanokura M. 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Miyazono K., Tabei N., Morita S., Ohnishi Y., Horinouchi S., Tanokura M. Accepted 2012-DEC-06 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. J. Bacteriol. (0021-9193) 2012 Only protein-protein interactions imex curation b1vzt1_strgg Glucokinase glkA Glucose kinase SGR_5377 protein protein strain jcm 4626 / nb Streptomyces griseus subsp. griseus MGLTIGVDIGGTKIAAGVVDEEGRILSTFKVATPPTAEGIVDAICAAVAGASEGHDVEAVGIGAAGYVDDKRATVLFAPNIDWRHEPLKDKVEQRVGLPVVVENDANAAAWGEYRFGAGQGHDDVICITLGTGLGGGIIIGNKLRRGRFGVAAEFGHIRVVPDGLLCGCGSQGCWEQYASGRALVRYAKQRANATPENAAVLLGLGDGSVDGIEGKHISEAARQGDPVAVDSFRELARWAGAGLADLASLFDPSAFIVGGGVSDEGELVLDPIRKSFRRWLIGGEWRPHAQVLAAQLGGKAGLVGAADLARQG A68A18695D637B25 glka-glka 1 3 unspecified role unspecified role unspecified role unspecified role physical association physical association figure 3 glka-glka-1 2 3 unspecified role unspecified role unspecified role unspecified role direct interaction direct interaction figure 3 3.25A Rworking = 0.21 Rfree = 0.26